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Protein

B-cell lymphoma 3 protein homolog

Gene

Bcl3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit (By similarity). In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation.By similarity1 Publication

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. sequence-specific DNA binding transcription factor activity Source: MGI
  3. transcription factor binding Source: MGI

GO - Biological processi

  1. antimicrobial humoral response Source: MGI
  2. cellular response to DNA damage stimulus Source: MGI
  3. defense response to bacterium Source: MGI
  4. defense response to protozoan Source: MGI
  5. DNA damage response, signal transduction by p53 class mediator Source: MGI
  6. extracellular matrix organization Source: MGI
  7. follicular dendritic cell differentiation Source: MGI
  8. germinal center formation Source: MGI
  9. humoral immune response mediated by circulating immunoglobulin Source: MGI
  10. I-kappaB kinase/NF-kappaB signaling Source: MGI
  11. intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: MGI
  12. marginal zone B cell differentiation Source: MGI
  13. negative regulation of apoptotic process Source: MGI
  14. negative regulation of interleukin-8 biosynthetic process Source: MGI
  15. negative regulation of transcription, DNA-templated Source: MGI
  16. negative regulation of tumor necrosis factor biosynthetic process Source: MGI
  17. positive regulation of interferon-gamma production Source: MGI
  18. positive regulation of interleukin-10 biosynthetic process Source: MGI
  19. positive regulation of transcription, DNA-templated Source: MGI
  20. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  21. positive regulation of translation Source: MGI
  22. protein import into nucleus, translocation Source: MGI
  23. regulation of apoptotic process Source: MGI
  24. regulation of DNA binding Source: Ensembl
  25. regulation of NF-kappaB import into nucleus Source: Ensembl
  26. response to UV-C Source: MGI
  27. response to virus Source: MGI
  28. spleen development Source: MGI
  29. T-helper 1 type immune response Source: MGI
  30. T-helper 2 cell differentiation Source: MGI
  31. transcription, DNA-templated Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
B-cell lymphoma 3 protein homolog
Short name:
BCL-3
Gene namesi
Name:Bcl3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:88140. Bcl3.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication. Cytoplasmperinuclear region 1 Publication
Note: Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation.

GO - Cellular componenti

  1. Bcl3/NF-kappaB2 complex Source: MGI
  2. Bcl3-Bcl10 complex Source: MGI
  3. cytoplasm Source: MGI
  4. intercellular bridge Source: MGI
  5. nucleoplasm Source: MGI
  6. nucleus Source: MGI
  7. perinuclear region of cytoplasm Source: UniProtKB-SubCell
  8. protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 448448B-cell lymphoma 3 protein homologPRO_0000066977Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei396 – 3961Phosphoserine; by GSK3By similarity
Modified residuei400 – 4001Phosphoserine; by GSK3By similarity

Post-translational modificationi

Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation.
Activated by phosphorylation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ9Z2F6.

PTM databases

PhosphoSiteiQ9Z2F6.

Expressioni

Gene expression databases

BgeeiQ9Z2F6.
CleanExiMM_BCL3.
ExpressionAtlasiQ9Z2F6. baseline and differential.
GenevestigatoriQ9Z2F6.

Interactioni

Subunit structurei

Component of a complex consisting of the NF-kappa-B p52-p52 homodimer and BCL3. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Interacts with N4BP2, COPS5 and PIR (By similarity). Interacts with CYLD.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CyldQ80TQ25EBI-943884,EBI-943859

Protein-protein interaction databases

BioGridi198326. 3 interactions.
IntActiQ9Z2F6. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2F6.
SMRiQ9Z2F6. Positions 128-412.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati129 – 16133ANK 1Add
BLAST
Repeati166 – 19530ANK 2Add
BLAST
Repeati199 – 22830ANK 3Add
BLAST
Repeati236 – 26530ANK 4Add
BLAST
Repeati270 – 29930ANK 5Add
BLAST
Repeati303 – 33230ANK 6Add
BLAST
Repeati333 – 36230ANK 7Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi358 – 44588Pro/Ser-richAdd
BLAST

Sequence similaritiesi

Contains 7 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00550000074527.
HOGENOMiHOG000095189.
HOVERGENiHBG108320.
InParanoidiQ9Z2F6.
KOiK09258.
OMAiPLYPMMC.
OrthoDBiEOG7W154S.
PhylomeDBiQ9Z2F6.
TreeFamiTF320166.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z2F6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRCPAGAMD EGPVDLRTRP KGTPGAALPL RKRPLRPASP EPATTRSPAG
60 70 80 90 100
PLDALRSGCD VPVVPGPPHC VARPEALYYQ GPLMPIYSTP TMAPHFPLLN
110 120 130 140 150
LPTHPYSMIC PMEHPLSADI AMATRVDEDG DTPLHIAVVQ NNIAAVYRIL
160 170 180 190 200
SLFKLGSREV DVHNNLRQTP LHLAVITTLP DMVRLLVTAG ASPMALDRHG
210 220 230 240 250
QTAIHLACEH RSPSCLQALL DSATSGSVDL EVRNYEGLTA LHVAVNTGCQ
260 270 280 290 300
EAVLLLLERG ADIDAVDIKS GRSPLIHAVE NNSLNMVQLL LLHGANVNAQ
310 320 330 340 350
MYSGSSALHS ASGRGLLPLV RTLVRSGADS GLKNCHNDTP LMVARSRRVI
360 370 380 390 400
DILRGKASRA ASGSQPEPSP DQSATNSPES SSRLSSNGLQ SSPSSSPSLS
410 420 430 440
PPKDAPGFPA TPQNFFLPTT STPAFLPFPG VLRGPGRPVP PSPAPGSS
Length:448
Mass (Da):47,216
Last modified:April 19, 2010 - v2
Checksum:i7665D28BBC3EE194
GO

Sequence cautioni

The sequence AAC79694.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC149085 Genomic DNA. No translation available.
AC149282 Genomic DNA. No translation available.
AF067774 mRNA. Translation: AAC79694.1. Different initiation.
CCDSiCCDS20914.2.
RefSeqiNP_291079.2. NM_033601.3.
UniGeneiMm.439658.

Genome annotation databases

EnsembliENSMUST00000120537; ENSMUSP00000113851; ENSMUSG00000053175.
GeneIDi12051.
KEGGimmu:12051.
UCSCiuc009fnk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC149085 Genomic DNA. No translation available.
AC149282 Genomic DNA. No translation available.
AF067774 mRNA. Translation: AAC79694.1. Different initiation.
CCDSiCCDS20914.2.
RefSeqiNP_291079.2. NM_033601.3.
UniGeneiMm.439658.

3D structure databases

ProteinModelPortaliQ9Z2F6.
SMRiQ9Z2F6. Positions 128-412.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198326. 3 interactions.
IntActiQ9Z2F6. 1 interaction.

PTM databases

PhosphoSiteiQ9Z2F6.

Proteomic databases

PRIDEiQ9Z2F6.

Protocols and materials databases

DNASUi12051.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000120537; ENSMUSP00000113851; ENSMUSG00000053175.
GeneIDi12051.
KEGGimmu:12051.
UCSCiuc009fnk.2. mouse.

Organism-specific databases

CTDi602.
MGIiMGI:88140. Bcl3.

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00550000074527.
HOGENOMiHOG000095189.
HOVERGENiHBG108320.
InParanoidiQ9Z2F6.
KOiK09258.
OMAiPLYPMMC.
OrthoDBiEOG7W154S.
PhylomeDBiQ9Z2F6.
TreeFamiTF320166.

Miscellaneous databases

NextBioi280347.
PROiQ9Z2F6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z2F6.
CleanExiMM_BCL3.
ExpressionAtlasiQ9Z2F6. baseline and differential.
GenevestigatoriQ9Z2F6.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "Interleukin-9 regulates NF-kappaB activity through BCL3 gene induction."
    Richard M., Louahed J., Demoulin J.-B., Renauld J.-C.
    Blood 93:4318-4327(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  3. "Cyld inhibits tumor cell proliferation by blocking Bcl-3-dependent NF-kappaB signaling."
    Massoumi R., Chmielarska K., Hennecke K., Pfeifer A., Fassler R.
    Cell 125:665-677(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CYLD, SUBCELLULAR LOCATION.
  4. "CYLD negatively regulates cell-cycle progression by inactivating HDAC6 and increasing the levels of acetylated tubulin."
    Wickstrom S.A., Masoumi K.C., Khochbin S., Fassler R., Massoumi R.
    EMBO J. 29:131-144(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CYLD.

Entry informationi

Entry nameiBCL3_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2F6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2004
Last sequence update: April 19, 2010
Last modified: March 3, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-9 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.