Q9Z2F6 (BCL3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
B-cell lymphoma 3 protein homolog
Short name=BCL-3

Gene names

Name:

Bcl3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit By similarity. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation. Ref.3
Subunit structure	Component of a complex consisting of the NF-kappa-B p52-p52 homodimer and BCL3. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Interacts with N4BP2, COPS5 and PIR By similarity. Interacts with CYLD. Ref.3 Ref.4
Subcellular location	Nucleus. Cytoplasm. Cytoplasm › perinuclear region. Note: Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Ref.3
Post-translational modification	Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation. Activated by phosphorylation By similarity.
Sequence similarities	Contains 7 ANK repeats.
Caution	It is uncertain whether Met-1 or Met-9 is the initiator.
Sequence caution	The sequence AAC79694.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	ANK repeat Repeat
Molecular function	Activator
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	T-helper 1 type immune response Inferred from mutant phenotype. Source: MGI T-helper 2 cell differentiation Inferred from mutant phenotype. Source: MGI antimicrobial humoral response Inferred from mutant phenotype. Source: MGI defense response to bacterium Inferred from mutant phenotype. Source: MGI defense response to protozoan Inferred from mutant phenotype. Source: MGI extracellular matrix organization Inferred from mutant phenotype. Source: MGI follicular dendritic cell differentiation Inferred from mutant phenotype. Source: MGI germinal center formation Inferred from mutant phenotype. Source: MGI humoral immune response mediated by circulating immunoglobulin Inferred from mutant phenotype. Source: MGI marginal zone B cell differentiation Inferred from mutant phenotype. Source: MGI negative regulation of apoptotic process Inferred from mutant phenotype. Source: MGI negative regulation of tumor necrosis factor biosynthetic process Inferred from mutant phenotype. Source: MGI positive regulation of interferon-gamma production Inferred from mutant phenotype. Source: MGI positive regulation of interleukin-10 biosynthetic process Inferred from mutant phenotype. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI spleen development Inferred from mutant phenotype. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from direct assay. Source: MGI sequence-specific DNA binding transcription factor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Cyld	Q80TQ2	4	EBI-943884,EBI-943859

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	B-cell lymphoma 3 protein homolog		PRO_0000066977
Regions
	Repeat	129 – 161	33	ANK 1
	Repeat	166 – 195	30	ANK 2
	Repeat	199 – 228	30	ANK 3
	Repeat	236 – 265	30	ANK 4
	Repeat	270 – 299	30	ANK 5
	Repeat	303 – 332	30	ANK 6
	Repeat	333 – 362	30	ANK 7
	Compositional bias	358 – 445	88	Pro/Ser-rich
Amino acid modifications
	Modified residue	396	1	Phosphoserine; by GSK3 By similarity
	Modified residue	400	1	Phosphoserine; by GSK3 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z2F6 [UniParc]. Last modified April 20, 2010. Version 2. Checksum: 7665D28BBC3EE194	FASTA	448	47,216
	10 20 30 40 50 60 MPRCPAGAMD EGPVDLRTRP KGTPGAALPL RKRPLRPASP EPATTRSPAG PLDALRSGCD 70 80 90 100 110 120 VPVVPGPPHC VARPEALYYQ GPLMPIYSTP TMAPHFPLLN LPTHPYSMIC PMEHPLSADI 130 140 150 160 170 180 AMATRVDEDG DTPLHIAVVQ NNIAAVYRIL SLFKLGSREV DVHNNLRQTP LHLAVITTLP 190 200 210 220 230 240 DMVRLLVTAG ASPMALDRHG QTAIHLACEH RSPSCLQALL DSATSGSVDL EVRNYEGLTA 250 260 270 280 290 300 LHVAVNTGCQ EAVLLLLERG ADIDAVDIKS GRSPLIHAVE NNSLNMVQLL LLHGANVNAQ 310 320 330 340 350 360 MYSGSSALHS ASGRGLLPLV RTLVRSGADS GLKNCHNDTP LMVARSRRVI DILRGKASRA 370 380 390 400 410 420 ASGSQPEPSP DQSATNSPES SSRLSSNGLQ SSPSSSPSLS PPKDAPGFPA TPQNFFLPTT 430 440 STPAFLPFPG VLRGPGRPVP PSPAPGSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[2]	"Interleukin-9 regulates NF-kappaB activity through BCL3 gene induction." Richard M., Louahed J., Demoulin J.-B., Renauld J.-C. Blood 93:4318-4327(1999) [PubMed: 10361130] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
[3]	"Cyld inhibits tumor cell proliferation by blocking Bcl-3-dependent NF-kappaB signaling." Massoumi R., Chmielarska K., Hennecke K., Pfeifer A., Fassler R. Cell 125:665-677(2006) [PubMed: 16713561] [Abstract] Cited for: FUNCTION, INTERACTION WITH CYLD, SUBCELLULAR LOCATION.
[4]	"CYLD negatively regulates cell-cycle progression by inactivating HDAC6 and increasing the levels of acetylated tubulin." Wickstrom S.A., Masoumi K.C., Khochbin S., Fassler R., Massoumi R. EMBO J. 29:131-144(2010) [PubMed: 19893491] [Abstract] Cited for: INTERACTION WITH CYLD.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC149085 Genomic DNA. No translation available. AC149282 Genomic DNA. No translation available. AF067774 mRNA. Translation: AAC79694.1. Different initiation.
IPI	IPI00131124.
RefSeq	NP_291079.2. NM_033601.3.
UniGene	Mm.439658.
3D structure databases
ProteinModelPortal	Q9Z2F6.
SMR	Q9Z2F6. Positions 128-355.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Z2F6. 1 interaction.
STRING	Q9Z2F6.
PTM databases
PhosphoSite	Q9Z2F6.
Proteomic databases
PRIDE	Q9Z2F6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000120537; ENSMUSP00000113851; ENSMUSG00000053175.
GeneID	12051.
KEGG	mmu:12051.
UCSC	uc009fnk.2. mouse.
Organism-specific databases
CTD	602.
MGI	MGI:88140. Bcl3.
Phylogenomic databases
GeneTree	ENSGT00550000074527.
HOGENOM	HBG506010.
HOVERGEN	HBG108320.
InParanoid	Q9Z2F6.
OrthoDB	EOG4NCMD1.
Gene expression databases
ArrayExpress	Q9Z2F6.
Bgee	Q9Z2F6.
CleanEx	MM_BCL3.
Genevestigator	Q9Z2F6.
GermOnline	ENSMUSG00000053175. Mus musculus.
Family and domain databases
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
KO	K09258.
Pfam	PF00023. Ank. 1 hit. PF12796. Ank_2. 1 hit. [Graphical view]
PRINTS	PR01415. ANKYRIN.
SMART	SM00248. ANK. 6 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 5 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	280347.
SOURCE	Search...

Entry information

Entry name

BCL3_MOUSE

Accession

Primary (citable) accession number: Q9Z2F6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	April 20, 2010
Last modified:	December 14, 2011
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program