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Protein

Seipin

Gene

Bscl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Is a regulator of lipid catabolism essential for adipocyte differentiation. Necessary for correct lipid storage and lipid droplets maintenance; may play a tissue-autonomous role in controlling lipid storage in adipocytes and in preventing ectopic lipid droplet formation in non-adipose tissues. May also be involved in the central regulation of energy homeostasis.4 Publications

GO - Biological processi

  • fat cell differentiation Source: UniProtKB
  • lipid catabolic process Source: UniProtKB-KW
  • lipid particle organization Source: MGI
  • lipid storage Source: MGI
  • negative regulation of lipid catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Seipin
Alternative name(s):
Bernardinelli-Seip congenital lipodystrophy type 2 protein homolog
Gene namesi
Name:Bscl2Imported
Synonyms:Gng3lgImported
ORF Names:MNCb-2630
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1298392. Bscl2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2727CytoplasmicSequence analysisAdd
BLAST
Transmembranei28 – 4821HelicalSequence analysisAdd
BLAST
Topological domaini49 – 242194LumenalSequence analysisAdd
BLAST
Transmembranei243 – 26321HelicalSequence analysisAdd
BLAST
Topological domaini264 – 383120CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice display increased early postnatal mortality rate, reduction of total body fat mass, nearly total absence of gonadal fat and great reduction in subcutaneous fat. Mice have enlarged visceral organs, markedly steatotic liver, decreased adipocyte size and number, and display decreased levels of plasma adiponectin and leptin, glucose intolerance and insulin resistance but not hypertriglyceridemia.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 383383SeipinPRO_0000191680Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi88 – 881N-linked (GlcNAc...)By similarity
Glycosylationi242 – 2421N-linked (GlcNAc...)Sequence analysis
Modified residuei289 – 2891PhosphoserineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9Z2E9.
MaxQBiQ9Z2E9.
PaxDbiQ9Z2E9.
PRIDEiQ9Z2E9.

PTM databases

iPTMnetiQ9Z2E9.
PhosphoSiteiQ9Z2E9.

Expressioni

Tissue specificityi

Expressed in the paraventricular nucleus of the hypothalamus (PVN) and brainstem dorsal vagal complex (DVC) in oxytocin and catecholaminergic neurons (at protein level). Highest expression detected in subcutaneous and epididymal white adipose tissue, brown adipose tissue and testis. Also expressed in brain, skeletal muscle and adrenal gland, with lower levels detected in liver, heart, kidney, spleen, lung and small intestine. In brain, detected in piriform cortex, olfactory tubercle, islands of Calleja, lateral septal nucleus, medial septal nucleus, nucleus of the vertical limb of the diagonal band, nucleus of the horizontal limb of the diagonal band, preoptic area, paraventricular thalamic nucleus, lateral globus pallidus, supraoptic nucleus, suprachiasmatic nucleus, subfornical organ, paraventricular nucleus of the hypothalamus, zona incerta, dorsomedial nucleus of the hypothalamus, ventromedial nucleus of the hypothalamus, arcuate nucleus of the hypothalamus, basomedial amygdaloid nucleus, medial amygdaloid nucleus, medial habenular, pyramidal cell layer of the hippocampus, granular layer of the dentate gyrus, posterior hypothalamus, supramammilliary nucleus, premammillary nucleus, nucleus of Darkschewitsch, Edinger-Westphal nucleus, ventral tegmental area, dorsal raphe nucleus, periaqueductal gray, median raphe nucleus, lateral parabrachial nucleus, dorsal tegmental nucleus, laterodorsal tegmental nucleus, locus coeruleus, Barrington's nucleus, medial vestibular nucleus, ambiguous nucleus, dorsal vagal complex and hypoglossal nucleus.4 Publications

Inductioni

Up-regulated during in vitro adipocyte differentiation.2 Publications

Gene expression databases

BgeeiQ9Z2E9.
ExpressionAtlasiQ9Z2E9. baseline and differential.
GenevisibleiQ9Z2E9. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000127685.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2E9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the seipin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4200. Eukaryota.
ENOG4111GRP. LUCA.
GeneTreeiENSGT00390000011639.
HOGENOMiHOG000220875.
HOVERGENiHBG050736.
InParanoidiQ9Z2E9.
KOiK19365.
OrthoDBiEOG7CCBS1.
TreeFamiTF314000.

Family and domain databases

InterProiIPR009617. Seipin.
[Graphical view]
PfamiPF06775. Seipin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z2E9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNDPPVPAL LWAQEVGHVL AGRARRLMLQ FGVLFCTILL LLWVSVFLYG
60 70 80 90 100
SFYYSYMPTV SHLSPVHFHY RTDCDSSTAS LCSFPVANVS LAKSGRDRVL
110 120 130 140 150
MYGQPYRVTL ELELPESPVN QDLGMFLVTV SCYTRGGRII STSSRSVMLH
160 170 180 190 200
YRSQLLQVLD TLLFSSLLLF GFAEQKQLLE VELYSDYREN SYVPTTGAII
210 220 230 240 250
EIHSKRIQMY GAYLRIHAHF TGLRYLLYNF PMTCAFVGVA SNFTFLSVIV
260 270 280 290 300
LFSYMQWVWG AVWPRHRFSL QVNIRQRDNS HHGAPRRISR HQPGQESTQQ
310 320 330 340 350
SDVTEDGESP EDPSGTEGQL SEEEKPEKRP LNGEEEQEPE ASDGSWEDAA
360 370 380
LLTEANPPTS ASASALAPET LGSLRQRPTC SSS
Length:383
Mass (Da):43,105
Last modified:October 1, 2000 - v2
Checksum:iAB185C29BC67634E
GO

Sequence cautioni

The sequence AAH61689.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAA95071.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE34722.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti341 – 3411A → V in BAA92759 (PubMed:10679242).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069954 mRNA. Translation: AAC77923.2.
AB030196 mRNA. Translation: BAA92759.1.
AB041588 mRNA. Translation: BAA95071.1. Different initiation.
AK151606 mRNA. Translation: BAE30545.1.
AK155753 mRNA. Translation: BAE33418.1.
AK158901 mRNA. Translation: BAE34722.1. Different initiation.
AK170183 mRNA. Translation: BAE41621.1.
BC043023 mRNA. Translation: AAH43023.2.
BC061689 mRNA. Translation: AAH61689.1. Different initiation.
CCDSiCCDS70931.1.
RefSeqiNP_001129536.1. NM_001136064.3.
NP_001277752.1. NM_001290823.1.
NP_032170.3. NM_008144.5.
XP_006526754.1. XM_006526691.1.
UniGeneiMm.345134.

Genome annotation databases

EnsembliENSMUST00000086058; ENSMUSP00000083224; ENSMUSG00000071657.
ENSMUST00000159634; ENSMUSP00000125422; ENSMUSG00000071657.
ENSMUST00000160556; ENSMUSP00000123976; ENSMUSG00000071657.
GeneIDi14705.
KEGGimmu:14705.
UCSCiuc008gni.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069954 mRNA. Translation: AAC77923.2.
AB030196 mRNA. Translation: BAA92759.1.
AB041588 mRNA. Translation: BAA95071.1. Different initiation.
AK151606 mRNA. Translation: BAE30545.1.
AK155753 mRNA. Translation: BAE33418.1.
AK158901 mRNA. Translation: BAE34722.1. Different initiation.
AK170183 mRNA. Translation: BAE41621.1.
BC043023 mRNA. Translation: AAH43023.2.
BC061689 mRNA. Translation: AAH61689.1. Different initiation.
CCDSiCCDS70931.1.
RefSeqiNP_001129536.1. NM_001136064.3.
NP_001277752.1. NM_001290823.1.
NP_032170.3. NM_008144.5.
XP_006526754.1. XM_006526691.1.
UniGeneiMm.345134.

3D structure databases

ProteinModelPortaliQ9Z2E9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000127685.

PTM databases

iPTMnetiQ9Z2E9.
PhosphoSiteiQ9Z2E9.

Proteomic databases

EPDiQ9Z2E9.
MaxQBiQ9Z2E9.
PaxDbiQ9Z2E9.
PRIDEiQ9Z2E9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000086058; ENSMUSP00000083224; ENSMUSG00000071657.
ENSMUST00000159634; ENSMUSP00000125422; ENSMUSG00000071657.
ENSMUST00000160556; ENSMUSP00000123976; ENSMUSG00000071657.
GeneIDi14705.
KEGGimmu:14705.
UCSCiuc008gni.2. mouse.

Organism-specific databases

CTDi26580.
MGIiMGI:1298392. Bscl2.

Phylogenomic databases

eggNOGiKOG4200. Eukaryota.
ENOG4111GRP. LUCA.
GeneTreeiENSGT00390000011639.
HOGENOMiHOG000220875.
HOVERGENiHBG050736.
InParanoidiQ9Z2E9.
KOiK19365.
OrthoDBiEOG7CCBS1.
TreeFamiTF314000.

Miscellaneous databases

PROiQ9Z2E9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z2E9.
ExpressionAtlasiQ9Z2E9. baseline and differential.
GenevisibleiQ9Z2E9. MM.

Family and domain databases

InterProiIPR009617. Seipin.
[Graphical view]
PfamiPF06775. Seipin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and mapping of the G protein gamma3 subunit gene and a divergently transcribed novel gene, Gng3lg."
    Downes G.B., Copeland N.G., Jenkins N.A., Gautam N.
    Genomics 53:220-230(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: BALB/cJImported.
    Tissue: BrainImported.
  2. Downes G.B., Gautam N.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO N-TERMINUS.
  3. "Growth suppression of Escherichia coli by induction of expression of mammalian genes with transmembrane or ATPase domains."
    Inoue S., Sano H., Ohta M.
    Biochem. Biophys. Res. Commun. 268:553-561(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: BrainImported.
  4. "Isolation of full-length cDNA clones from mouse brain cDNA library made by oligo-capping method."
    Osada N., Kusuda J., Tanuma R., Ito A., Hirata M., Sugano S., Hashimoto K.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6JImported.
    Tissue: BrainImported.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow macrophage, Dendritic cell and Visual cortex.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain and Olfactory epitheliumImported.
  7. "The human lipodystrophy gene BSCL2/seipin may be essential for normal adipocyte differentiation."
    Payne V.A., Grimsey N., Tuthill A., Virtue S., Gray S.L., Dalla Nora E., Semple R.K., O'Rahilly S., Rochford J.J.
    Diabetes 57:2055-2060(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
  8. "The human lipodystrophy gene product Berardinelli-Seip congenital lipodystrophy 2/seipin plays a key role in adipocyte differentiation."
    Chen W., Yechoor V.K., Chang B.H., Li M.V., March K.L., Chan L.
    Endocrinology 150:4552-4561(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Lung, Pancreas, Spleen and Testis.
  10. "Seipin ablation in mice results in severe generalized lipodystrophy."
    Cui X., Wang Y., Tang Y., Liu Y., Zhao L., Deng J., Xu G., Peng X., Ju S., Liu G., Yang H.
    Hum. Mol. Genet. 20:3022-3030(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  11. "Berardinelli-seip congenital lipodystrophy 2/seipin is a cell-autonomous regulator of lipolysis essential for adipocyte differentiation."
    Chen W., Chang B., Saha P., Hartig S.M., Li L., Reddy V.T., Yang Y., Yechoor V., Mancini M.A., Chan L.
    Mol. Cell. Biol. 32:1099-1111(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  12. "Neuroanatomical characterisation of the expression of the lipodystrophy and motor-neuropathy gene Bscl2 in adult mouse brain."
    Garfield A.S., Chan W.S., Dennis R.J., Ito D., Heisler L.K., Rochford J.J.
    PLoS ONE 7:E45790-E45790(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiBSCL2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2E9
Secondary accession number(s): Q3TY41
, Q3U1S5, Q810B0, Q9JJC2, Q9JMF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.