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Protein

Serine/threonine-protein kinase/endoribonuclease IRE2

Gene

Ern2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Role in expression of the DDIT3 transcription factor, required for the unfolded-protein response, growth arrest and apoptosis. Has no effect on 28S ribosomal RNA cleavage, unlike the corresponding human protein.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Enzyme regulationi

The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei536 – 5361ATPPROSITE-ProRule annotationBy similarity
Active sitei625 – 6251Proton acceptorPROSITE-ProRule annotationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi514 – 5229ATPPROSITE-ProRule annotationBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. endonuclease activity Source: MGI
  3. magnesium ion binding Source: UniProtKB
  4. protein serine/threonine kinase activity Source: UniProtKB
  5. ribonuclease activity Source: InterPro

GO - Biological processi

  1. activation of signaling protein activity involved in unfolded protein response Source: UniProtKB
  2. apoptotic chromosome condensation Source: MGI
  3. cell cycle arrest Source: UniProtKB
  4. intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: MGI
  5. mRNA processing Source: InterPro
  6. negative regulation of transcription, DNA-templated Source: MGI
  7. nucleic acid phosphodiester bond hydrolysis Source: MGI
  8. protein phosphorylation Source: UniProtKB
  9. regulation of transcription, DNA-templated Source: UniProtKB
  10. response to endoplasmic reticulum stress Source: MGI
  11. rRNA catabolic process Source: MGI
  12. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, Transcription, Transcription regulation, Unfolded protein response

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase/endoribonuclease IRE2
Alternative name(s):
Endoplasmic reticulum-to-nucleus signaling 2
Inositol-requiring protein 2
Ire1-beta
Short name:
IRE1b
Short name:
mIre1
Including the following 2 domains:
Gene namesi
Name:Ern2Imported
Synonyms:Ire2By similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1349436. Ern2.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Single-pass type I membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini35 – 426392LumenalSequence AnalysisAdd
BLAST
Transmembranei427 – 44721HelicalSequence AnalysisAdd
BLAST
Topological domaini448 – 911464CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. IRE1-TRAF2-ASK1 complex Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence AnalysisAdd
BLAST
Chaini34 – 911878Serine/threonine-protein kinase/endoribonuclease IRE2PRO_0000024330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9Z2E3.
PRIDEiQ9Z2E3.

PTM databases

PhosphoSiteiQ9Z2E3.

Expressioni

Gene expression databases

BgeeiQ9Z2E3.
ExpressionAtlasiQ9Z2E3. differential.
GenevestigatoriQ9Z2E3.

Interactioni

Protein-protein interaction databases

BioGridi205062. 3 interactions.
STRINGi10090.ENSMUSP00000033153.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2E3.
SMRiQ9Z2E3. Positions 36-310, 472-897.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini508 – 768261Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini771 – 899129KENPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 KEN domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00390000015684.
HOGENOMiHOG000012929.
HOVERGENiHBG051506.
InParanoidiQ9Z2E3.
KOiK11715.
OMAiMLRVHPT.
OrthoDBiEOG7C2R0H.
TreeFamiTF313986.

Family and domain databases

Gene3Di2.140.10.10. 1 hit.
InterProiIPR010513. KEN_dom.
IPR011009. Kinase-like_dom.
IPR018391. PQQ_beta_propeller_repeat.
IPR002372. PQQ_repeat.
IPR000719. Prot_kinase_dom.
IPR006567. PUG-dom.
IPR027295. Quinonprotein_ADH-like_fam.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF01011. PQQ. 1 hit.
PF06479. Ribonuc_2-5A. 1 hit.
[Graphical view]
SMARTiSM00564. PQQ. 4 hits.
SM00580. PUG. 1 hit.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51392. KEN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z2E3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARPVQRFQL WSPLGFLLQL VTLLGKLGPQ VQSVRPESLL FVSTLDGSLH
60 70 80 90 100
ALNKQTGDLK WTVKDDPIIQ GPMYVTEMAF LSDPADGSLY VLGTQKQQGL
110 120 130 140 150
MKLPFTIPEL VHASPCRSSD GVFYTGRKQD AWFVVDPESG ETQMTLTTEG
160 170 180 190 200
LSTPQLFIGR TQYTVSMHDL RTPALRWNTT YRRYSAPLLN GSPGKYMSHL
210 220 230 240 250
TSCGMGLLLT VDPGSGIVLW TQDLGVPVTG IYTWHQDGLH QLPHLTLARD
260 270 280 290 300
TLHFLVLRWG HIRLPASSYQ DTATQFSSLD TQLLMTLYVG KEEAGFYVSK
310 320 330 340 350
ALVHAGVALV PRGLTLAPMD GPTTDEVTLQ VSGEREGSPS TAVRYPSGSV
360 370 380 390 400
ALPSQWLLIG YHEPPPVLHT TMLRVHPIPG KVSAETRASE DLHAPPVFFE
410 420 430 440 450
LLNLRREDPE LHPEEKASDS YPGLGSQDLL AATFTAILLG AWVLYLMRQQ
460 470 480 490 500
QQSPSAPAGP PDLSQDAQGQ LSRDILQDQR RFQSPSEPAQ PPHDPEAGQP
510 520 530 540 550
TVVGKISFNP KDVLGRGAGG TFVFRGQFEG RAVAVKRLLR ECFGLVRREV
560 570 580 590 600
QLLQESDRHP NVLRYFCTEH GPQFHYIALE LCQASLQEYV ESPDLDRWGL
610 620 630 640 650
EPTTVLQQMM SGLAHLHSLH IVHRDLKPAN ILMAGPDSQG QGRVVISDFG
660 670 680 690 700
LCKKLPVGRC SFSLHSGIPG TEGWMAPELL QLPPDSPTSA VDIFSAGCVF
710 720 730 740 750
YYVLSGGSHP FGESLYRQAN ILSGDPCLAQ LQEETHDKVV ALDLVRAMLS
760 770 780 790 800
LLPQDRPSAG WVLAHPLFWS RAKELQFFQD VSDWLEKEPD QGPLVSALEA
810 820 830 840 850
GSYKVVREDW HKHISAPLQA DLKRFRSYKG TSVRDLLRAM RNKKHHYREL
860 870 880 890 900
PAEVRQTLGQ LPAGFIQYFT QRFPRLLLHT HRAMRTCASE SLFLPYYPPA
910
LEARRPDATK S
Length:911
Mass (Da):101,262
Last modified:July 27, 2011 - v2
Checksum:i77E210F1D8D15737
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451L → W in AAC64400 (PubMed:9755171).Curated
Sequence conflicti95 – 951Q → H in AAC64400 (PubMed:9755171).Curated
Sequence conflicti97 – 971Q → L in AAC64400 (PubMed:9755171).Curated
Sequence conflicti689 – 6891S → N in AAC64400 (PubMed:9755171).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071777 mRNA. Translation: AAC64400.1.
AK153659 mRNA. Translation: BAE32136.1.
CH466531 Genomic DNA. Translation: EDL17275.1.
CCDSiCCDS21813.1.
RefSeqiNP_036146.2. NM_012016.2.
UniGeneiMm.20452.

Genome annotation databases

EnsembliENSMUST00000033153; ENSMUSP00000033153; ENSMUSG00000030866.
GeneIDi26918.
KEGGimmu:26918.
UCSCiuc009joq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071777 mRNA. Translation: AAC64400.1.
AK153659 mRNA. Translation: BAE32136.1.
CH466531 Genomic DNA. Translation: EDL17275.1.
CCDSiCCDS21813.1.
RefSeqiNP_036146.2. NM_012016.2.
UniGeneiMm.20452.

3D structure databases

ProteinModelPortaliQ9Z2E3.
SMRiQ9Z2E3. Positions 36-310, 472-897.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205062. 3 interactions.
STRINGi10090.ENSMUSP00000033153.

PTM databases

PhosphoSiteiQ9Z2E3.

Proteomic databases

MaxQBiQ9Z2E3.
PRIDEiQ9Z2E3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033153; ENSMUSP00000033153; ENSMUSG00000030866.
GeneIDi26918.
KEGGimmu:26918.
UCSCiuc009joq.1. mouse.

Organism-specific databases

CTDi10595.
MGIiMGI:1349436. Ern2.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00390000015684.
HOGENOMiHOG000012929.
HOVERGENiHBG051506.
InParanoidiQ9Z2E3.
KOiK11715.
OMAiMLRVHPT.
OrthoDBiEOG7C2R0H.
TreeFamiTF313986.

Miscellaneous databases

NextBioi304797.
PROiQ9Z2E3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z2E3.
ExpressionAtlasiQ9Z2E3. differential.
GenevestigatoriQ9Z2E3.

Family and domain databases

Gene3Di2.140.10.10. 1 hit.
InterProiIPR010513. KEN_dom.
IPR011009. Kinase-like_dom.
IPR018391. PQQ_beta_propeller_repeat.
IPR002372. PQQ_repeat.
IPR000719. Prot_kinase_dom.
IPR006567. PUG-dom.
IPR027295. Quinonprotein_ADH-like_fam.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF01011. PQQ. 1 hit.
PF06479. Ribonuc_2-5A. 1 hit.
[Graphical view]
SMARTiSM00564. PQQ. 4 hits.
SM00580. PUG. 1 hit.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51392. KEN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of mammalian Ire1 reveals a diversity in the ER stress responses."
    Wang X.-Z., Harding H.P., Zhang Y., Jolicoeur E.M., Kuroda M., Ron D.
    EMBO J. 17:5708-5717(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Thymus.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiERN2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2E3
Secondary accession number(s): Q3U5E3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 27, 2011
Last modified: April 1, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.