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Q9Z2E3

- ERN2_MOUSE

UniProt

Q9Z2E3 - ERN2_MOUSE

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Protein

Serine/threonine-protein kinase/endoribonuclease IRE2

Gene
Ern2, Ire2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Role in expression of the DDIT3 transcription factor, required for the unfolded-protein response, growth arrest and apoptosis. Has no effect on 28S ribosomal RNA cleavage, unlike the corresponding human protein.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Magnesium By similarity.By similarity

Enzyme regulationi

The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei536 – 5361ATP By similarityBy similarity
Active sitei625 – 6251Proton acceptor By similarityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi514 – 5229ATP By similarityBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. endonuclease activity Source: Ensembl
  3. magnesium ion binding Source: UniProtKB
  4. protein serine/threonine kinase activity Source: UniProtKB
  5. ribonuclease activity Source: InterPro

GO - Biological processi

  1. activation of signaling protein activity involved in unfolded protein response Source: UniProtKB
  2. apoptotic chromosome condensation Source: Ensembl
  3. cell cycle arrest Source: UniProtKB
  4. intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: MGI
  5. mRNA processing Source: InterPro
  6. negative regulation of transcription, DNA-templated Source: Ensembl
  7. protein phosphorylation Source: UniProtKB
  8. regulation of transcription, DNA-templated Source: UniProtKB
  9. response to endoplasmic reticulum stress Source: MGI
  10. rRNA catabolic process Source: Ensembl
  11. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, Transcription, Transcription regulation, Unfolded protein response

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase/endoribonuclease IRE2
Alternative name(s):
Endoplasmic reticulum-to-nucleus signaling 2
Inositol-requiring protein 2
Ire1-beta
Short name:
IRE1b
Short name:
mIre1
Including the following 2 domains:
Gene namesi
Name:Ern2
Synonyms:Ire2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1349436. Ern2.

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass type I membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini35 – 426392Lumenal Reviewed predictionAdd
BLAST
Transmembranei427 – 44721Helical; Reviewed predictionAdd
BLAST
Topological domaini448 – 911464Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333 Reviewed predictionAdd
BLAST
Chaini34 – 911878Serine/threonine-protein kinase/endoribonuclease IRE2PRO_0000024330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi178 – 1781N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Autophosphorylated By similarity.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9Z2E3.
PRIDEiQ9Z2E3.

PTM databases

PhosphoSiteiQ9Z2E3.

Expressioni

Gene expression databases

ArrayExpressiQ9Z2E3.
BgeeiQ9Z2E3.
GenevestigatoriQ9Z2E3.

Interactioni

Protein-protein interaction databases

BioGridi205062. 3 interactions.
STRINGi10090.ENSMUSP00000033153.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2E3.
SMRiQ9Z2E3. Positions 36-310, 472-897.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini508 – 768261Protein kinaseAdd
BLAST
Domaini771 – 899129KENAdd
BLAST

Sequence similaritiesi

Contains 1 KEN domain.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00390000015684.
HOGENOMiHOG000012929.
HOVERGENiHBG051506.
InParanoidiQ3U5E3.
KOiK11715.
OMAiMLRVHPT.
OrthoDBiEOG7C2R0H.
TreeFamiTF313986.

Family and domain databases

Gene3Di2.140.10.10. 1 hit.
InterProiIPR010513. KEN_dom.
IPR011009. Kinase-like_dom.
IPR018391. PQQ_beta_propeller_repeat.
IPR002372. PQQ_repeat.
IPR000719. Prot_kinase_dom.
IPR006567. PUG-dom.
IPR027295. Quinonprotein_ADH-like_fam.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF01011. PQQ. 1 hit.
PF06479. Ribonuc_2-5A. 1 hit.
[Graphical view]
SMARTiSM00564. PQQ. 4 hits.
SM00580. PUG. 1 hit.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51392. KEN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z2E3-1 [UniParc]FASTAAdd to Basket

« Hide

MARPVQRFQL WSPLGFLLQL VTLLGKLGPQ VQSVRPESLL FVSTLDGSLH    50
ALNKQTGDLK WTVKDDPIIQ GPMYVTEMAF LSDPADGSLY VLGTQKQQGL 100
MKLPFTIPEL VHASPCRSSD GVFYTGRKQD AWFVVDPESG ETQMTLTTEG 150
LSTPQLFIGR TQYTVSMHDL RTPALRWNTT YRRYSAPLLN GSPGKYMSHL 200
TSCGMGLLLT VDPGSGIVLW TQDLGVPVTG IYTWHQDGLH QLPHLTLARD 250
TLHFLVLRWG HIRLPASSYQ DTATQFSSLD TQLLMTLYVG KEEAGFYVSK 300
ALVHAGVALV PRGLTLAPMD GPTTDEVTLQ VSGEREGSPS TAVRYPSGSV 350
ALPSQWLLIG YHEPPPVLHT TMLRVHPIPG KVSAETRASE DLHAPPVFFE 400
LLNLRREDPE LHPEEKASDS YPGLGSQDLL AATFTAILLG AWVLYLMRQQ 450
QQSPSAPAGP PDLSQDAQGQ LSRDILQDQR RFQSPSEPAQ PPHDPEAGQP 500
TVVGKISFNP KDVLGRGAGG TFVFRGQFEG RAVAVKRLLR ECFGLVRREV 550
QLLQESDRHP NVLRYFCTEH GPQFHYIALE LCQASLQEYV ESPDLDRWGL 600
EPTTVLQQMM SGLAHLHSLH IVHRDLKPAN ILMAGPDSQG QGRVVISDFG 650
LCKKLPVGRC SFSLHSGIPG TEGWMAPELL QLPPDSPTSA VDIFSAGCVF 700
YYVLSGGSHP FGESLYRQAN ILSGDPCLAQ LQEETHDKVV ALDLVRAMLS 750
LLPQDRPSAG WVLAHPLFWS RAKELQFFQD VSDWLEKEPD QGPLVSALEA 800
GSYKVVREDW HKHISAPLQA DLKRFRSYKG TSVRDLLRAM RNKKHHYREL 850
PAEVRQTLGQ LPAGFIQYFT QRFPRLLLHT HRAMRTCASE SLFLPYYPPA 900
LEARRPDATK S 911
Length:911
Mass (Da):101,262
Last modified:July 27, 2011 - v2
Checksum:i77E210F1D8D15737
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451L → W in AAC64400. 1 Publication
Sequence conflicti95 – 951Q → H in AAC64400. 1 Publication
Sequence conflicti97 – 971Q → L in AAC64400. 1 Publication
Sequence conflicti689 – 6891S → N in AAC64400. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF071777 mRNA. Translation: AAC64400.1.
AK153659 mRNA. Translation: BAE32136.1.
CH466531 Genomic DNA. Translation: EDL17275.1.
CCDSiCCDS21813.1.
RefSeqiNP_036146.2. NM_012016.2.
UniGeneiMm.20452.

Genome annotation databases

EnsembliENSMUST00000033153; ENSMUSP00000033153; ENSMUSG00000030866.
GeneIDi26918.
KEGGimmu:26918.
UCSCiuc009joq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF071777 mRNA. Translation: AAC64400.1 .
AK153659 mRNA. Translation: BAE32136.1 .
CH466531 Genomic DNA. Translation: EDL17275.1 .
CCDSi CCDS21813.1.
RefSeqi NP_036146.2. NM_012016.2.
UniGenei Mm.20452.

3D structure databases

ProteinModelPortali Q9Z2E3.
SMRi Q9Z2E3. Positions 36-310, 472-897.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 205062. 3 interactions.
STRINGi 10090.ENSMUSP00000033153.

PTM databases

PhosphoSitei Q9Z2E3.

Proteomic databases

MaxQBi Q9Z2E3.
PRIDEi Q9Z2E3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033153 ; ENSMUSP00000033153 ; ENSMUSG00000030866 .
GeneIDi 26918.
KEGGi mmu:26918.
UCSCi uc009joq.1. mouse.

Organism-specific databases

CTDi 10595.
MGIi MGI:1349436. Ern2.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00390000015684.
HOGENOMi HOG000012929.
HOVERGENi HBG051506.
InParanoidi Q3U5E3.
KOi K11715.
OMAi MLRVHPT.
OrthoDBi EOG7C2R0H.
TreeFami TF313986.

Miscellaneous databases

NextBioi 304797.
PROi Q9Z2E3.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9Z2E3.
Bgeei Q9Z2E3.
Genevestigatori Q9Z2E3.

Family and domain databases

Gene3Di 2.140.10.10. 1 hit.
InterProi IPR010513. KEN_dom.
IPR011009. Kinase-like_dom.
IPR018391. PQQ_beta_propeller_repeat.
IPR002372. PQQ_repeat.
IPR000719. Prot_kinase_dom.
IPR006567. PUG-dom.
IPR027295. Quinonprotein_ADH-like_fam.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
PF01011. PQQ. 1 hit.
PF06479. Ribonuc_2-5A. 1 hit.
[Graphical view ]
SMARTi SM00564. PQQ. 4 hits.
SM00580. PUG. 1 hit.
[Graphical view ]
SUPFAMi SSF50998. SSF50998. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51392. KEN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of mammalian Ire1 reveals a diversity in the ER stress responses."
    Wang X.-Z., Harding H.P., Zhang Y., Jolicoeur E.M., Kuroda M., Ron D.
    EMBO J. 17:5708-5717(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Thymus.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiERN2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2E3
Secondary accession number(s): Q3U5E3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi