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Protein

Serine/threonine-protein kinase/endoribonuclease IRE2

Gene

Ern2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Role in expression of the DDIT3 transcription factor, required for the unfolded-protein response, growth arrest and apoptosis. Has no effect on 28S ribosomal RNA cleavage, unlike the corresponding human protein.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Enzyme regulationi

The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei536ATPPROSITE-ProRule annotationBy similarity1
Active sitei625Proton acceptorPROSITE-ProRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi514 – 522ATPPROSITE-ProRule annotationBy similarity9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, Transcription, Transcription regulation, Unfolded protein response

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase/endoribonuclease IRE2
Alternative name(s):
Endoplasmic reticulum-to-nucleus signaling 2
Inositol-requiring protein 2
Ire1-beta
Short name:
IRE1b
Short name:
mIre1
Including the following 2 domains:
Gene namesi
Name:Ern2Imported
Synonyms:Ire2By similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1349436. Ern2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini35 – 426LumenalSequence analysisAdd BLAST392
Transmembranei427 – 447HelicalSequence analysisAdd BLAST21
Topological domaini448 – 911CytoplasmicSequence analysisAdd BLAST464

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • IRE1-TRAF2-ASK1 complex Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000002433034 – 911Serine/threonine-protein kinase/endoribonuclease IRE2Add BLAST878

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi178N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9Z2E3.
PaxDbiQ9Z2E3.
PeptideAtlasiQ9Z2E3.
PRIDEiQ9Z2E3.

PTM databases

PhosphoSitePlusiQ9Z2E3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030866.
ExpressionAtlasiQ9Z2E3. baseline and differential.
GenevisibleiQ9Z2E3. MM.

Interactioni

Protein-protein interaction databases

BioGridi205062. 3 interactors.
STRINGi10090.ENSMUSP00000033153.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2E3.
SMRiQ9Z2E3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini508 – 768Protein kinasePROSITE-ProRule annotationAdd BLAST261
Domaini771 – 899KENPROSITE-ProRule annotationAdd BLAST129

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 KEN domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1027. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00390000015684.
HOGENOMiHOG000012929.
HOVERGENiHBG051506.
InParanoidiQ9Z2E3.
KOiK11715.
OMAiETGFYVS.
OrthoDBiEOG091G049V.
TreeFamiTF313986.

Family and domain databases

Gene3Di2.140.10.10. 1 hit.
InterProiIPR033523. IRE2.
IPR010513. KEN_dom.
IPR011009. Kinase-like_dom.
IPR018391. PQQ_beta_propeller_repeat.
IPR000719. Prot_kinase_dom.
IPR018997. PUB_domain.
IPR027295. Quinoprotein_ADH-like_fam.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR13954:SF15. PTHR13954:SF15. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
PF06479. Ribonuc_2-5A. 1 hit.
[Graphical view]
SMARTiSM00564. PQQ. 4 hits.
SM00580. PUG. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51392. KEN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z2E3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARPVQRFQL WSPLGFLLQL VTLLGKLGPQ VQSVRPESLL FVSTLDGSLH
60 70 80 90 100
ALNKQTGDLK WTVKDDPIIQ GPMYVTEMAF LSDPADGSLY VLGTQKQQGL
110 120 130 140 150
MKLPFTIPEL VHASPCRSSD GVFYTGRKQD AWFVVDPESG ETQMTLTTEG
160 170 180 190 200
LSTPQLFIGR TQYTVSMHDL RTPALRWNTT YRRYSAPLLN GSPGKYMSHL
210 220 230 240 250
TSCGMGLLLT VDPGSGIVLW TQDLGVPVTG IYTWHQDGLH QLPHLTLARD
260 270 280 290 300
TLHFLVLRWG HIRLPASSYQ DTATQFSSLD TQLLMTLYVG KEEAGFYVSK
310 320 330 340 350
ALVHAGVALV PRGLTLAPMD GPTTDEVTLQ VSGEREGSPS TAVRYPSGSV
360 370 380 390 400
ALPSQWLLIG YHEPPPVLHT TMLRVHPIPG KVSAETRASE DLHAPPVFFE
410 420 430 440 450
LLNLRREDPE LHPEEKASDS YPGLGSQDLL AATFTAILLG AWVLYLMRQQ
460 470 480 490 500
QQSPSAPAGP PDLSQDAQGQ LSRDILQDQR RFQSPSEPAQ PPHDPEAGQP
510 520 530 540 550
TVVGKISFNP KDVLGRGAGG TFVFRGQFEG RAVAVKRLLR ECFGLVRREV
560 570 580 590 600
QLLQESDRHP NVLRYFCTEH GPQFHYIALE LCQASLQEYV ESPDLDRWGL
610 620 630 640 650
EPTTVLQQMM SGLAHLHSLH IVHRDLKPAN ILMAGPDSQG QGRVVISDFG
660 670 680 690 700
LCKKLPVGRC SFSLHSGIPG TEGWMAPELL QLPPDSPTSA VDIFSAGCVF
710 720 730 740 750
YYVLSGGSHP FGESLYRQAN ILSGDPCLAQ LQEETHDKVV ALDLVRAMLS
760 770 780 790 800
LLPQDRPSAG WVLAHPLFWS RAKELQFFQD VSDWLEKEPD QGPLVSALEA
810 820 830 840 850
GSYKVVREDW HKHISAPLQA DLKRFRSYKG TSVRDLLRAM RNKKHHYREL
860 870 880 890 900
PAEVRQTLGQ LPAGFIQYFT QRFPRLLLHT HRAMRTCASE SLFLPYYPPA
910
LEARRPDATK S
Length:911
Mass (Da):101,262
Last modified:July 27, 2011 - v2
Checksum:i77E210F1D8D15737
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45L → W in AAC64400 (PubMed:9755171).Curated1
Sequence conflicti95Q → H in AAC64400 (PubMed:9755171).Curated1
Sequence conflicti97Q → L in AAC64400 (PubMed:9755171).Curated1
Sequence conflicti689S → N in AAC64400 (PubMed:9755171).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071777 mRNA. Translation: AAC64400.1.
AK153659 mRNA. Translation: BAE32136.1.
CH466531 Genomic DNA. Translation: EDL17275.1.
CCDSiCCDS21813.1.
RefSeqiNP_036146.2. NM_012016.3.
UniGeneiMm.20452.

Genome annotation databases

EnsembliENSMUST00000033153; ENSMUSP00000033153; ENSMUSG00000030866.
GeneIDi26918.
KEGGimmu:26918.
UCSCiuc009joq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071777 mRNA. Translation: AAC64400.1.
AK153659 mRNA. Translation: BAE32136.1.
CH466531 Genomic DNA. Translation: EDL17275.1.
CCDSiCCDS21813.1.
RefSeqiNP_036146.2. NM_012016.3.
UniGeneiMm.20452.

3D structure databases

ProteinModelPortaliQ9Z2E3.
SMRiQ9Z2E3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205062. 3 interactors.
STRINGi10090.ENSMUSP00000033153.

PTM databases

PhosphoSitePlusiQ9Z2E3.

Proteomic databases

MaxQBiQ9Z2E3.
PaxDbiQ9Z2E3.
PeptideAtlasiQ9Z2E3.
PRIDEiQ9Z2E3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033153; ENSMUSP00000033153; ENSMUSG00000030866.
GeneIDi26918.
KEGGimmu:26918.
UCSCiuc009joq.1. mouse.

Organism-specific databases

CTDi10595.
MGIiMGI:1349436. Ern2.

Phylogenomic databases

eggNOGiKOG1027. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00390000015684.
HOGENOMiHOG000012929.
HOVERGENiHBG051506.
InParanoidiQ9Z2E3.
KOiK11715.
OMAiETGFYVS.
OrthoDBiEOG091G049V.
TreeFamiTF313986.

Miscellaneous databases

PROiQ9Z2E3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030866.
ExpressionAtlasiQ9Z2E3. baseline and differential.
GenevisibleiQ9Z2E3. MM.

Family and domain databases

Gene3Di2.140.10.10. 1 hit.
InterProiIPR033523. IRE2.
IPR010513. KEN_dom.
IPR011009. Kinase-like_dom.
IPR018391. PQQ_beta_propeller_repeat.
IPR000719. Prot_kinase_dom.
IPR018997. PUB_domain.
IPR027295. Quinoprotein_ADH-like_fam.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR13954:SF15. PTHR13954:SF15. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
PF06479. Ribonuc_2-5A. 1 hit.
[Graphical view]
SMARTiSM00564. PQQ. 4 hits.
SM00580. PUG. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51392. KEN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERN2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2E3
Secondary accession number(s): Q3U5E3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.