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Q9Z2E1

- MBD2_MOUSE

UniProt

Q9Z2E1 - MBD2_MOUSE

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Protein

Methyl-CpG-binding domain protein 2

Gene

Mbd2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression (By similarity). May enhance the activation of some unmethylated cAMP-responsive promoters (By similarity). Selectively represses transcription activity of methylated rRNA promoters.By similarity2 Publications

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. DNA binding Source: MGI
  3. methyl-CpG binding Source: MGI
  4. mRNA binding Source: MGI
  5. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: Ensembl
  6. RNA polymerase II distal enhancer sequence-specific DNA binding Source: Ensembl
  7. siRNA binding Source: MGI

GO - Biological processi

  1. ATP-dependent chromatin remodeling Source: Ensembl
  2. cellular protein complex assembly Source: MGI
  3. maternal behavior Source: MGI
  4. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  5. positive regulation of Wnt signaling pathway Source: MGI
  6. regulation of cell proliferation Source: MGI
  7. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_214440. NoRC negatively regulates rRNA expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Methyl-CpG-binding domain protein 2
Alternative name(s):
Methyl-CpG-binding protein MBD2
Gene namesi
Name:Mbd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:1333813. Mbd2.

Subcellular locationi

Nucleus By similarity
Note: Nuclear, in discrete foci. Detected at replication foci in late S phase (By similarity).By similarity

GO - Cellular componenti

  1. chromatin Source: MGI
  2. cytoplasm Source: MGI
  3. heterochromatin Source: MGI
  4. histone deacetylase complex Source: MGI
  5. nuclear chromatin Source: Ensembl
  6. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 414414Methyl-CpG-binding domain protein 2PRO_0000096261Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei184 – 1841PhosphoserineBy similarity
Modified residuei410 – 4101PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z2E1.
PaxDbiQ9Z2E1.
PRIDEiQ9Z2E1.

PTM databases

PhosphoSiteiQ9Z2E1.

Expressioni

Tissue specificityi

Highly expressed in brain, heart, kidney, lung, skeletal muscle, spleen and testis. Detected at lower levels in embryonic stem cells.1 Publication

Gene expression databases

BgeeiQ9Z2E1.
CleanExiMM_MBD2.
ExpressionAtlasiQ9Z2E1. baseline and differential.
GenevestigatoriQ9Z2E1.

Interactioni

Subunit structurei

Heterodimer with MBD3. Component of the MeCP1 complex that contains HDAC1 and HDAC2. Binds DNMT1, MIZF, GPN1, SIN3A, GATAD2A/p66-alpha and GATAD2B/p66-beta (By similarity). Interacts with DHX9 (By similarity).By similarity

Protein-protein interaction databases

BioGridi201331. 6 interactions.
IntActiQ9Z2E1. 4 interactions.
MINTiMINT-4101332.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2E1.
SMRiQ9Z2E1. Positions 152-216, 363-396.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini148 – 21669MBDPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi6 – 143138Gly-richAdd
BLAST
Compositional biasi50 – 9647Arg-richAdd
BLAST

Sequence similaritiesi

Contains 1 MBD (methyl-CpG-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG316386.
GeneTreeiENSGT00410000025376.
HOGENOMiHOG000013073.
HOVERGENiHBG052417.
InParanoidiQ9Z2E1.
KOiK11590.
OMAiNCISICC.
OrthoDBiEOG7QNVMV.
TreeFamiTF325032.

Family and domain databases

Gene3Di3.30.890.10. 2 hits.
InterProiIPR016177. DNA-bd_dom.
IPR025884. MeCpG-bd_2/3_C_dom.
IPR001739. Methyl_CpG_DNA-bd.
[Graphical view]
PfamiPF01429. MBD. 1 hit.
PF14048. MBD_C. 1 hit.
[Graphical view]
SMARTiSM00391. MBD. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS50982. MBD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9Z2E1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRAHPGGGRC CPEQEEGESA AGGSGAGGDS AIEQGGQGSA LAPSPVSGVR
60 70 80 90 100
REGARGGGRG RGRWKQAARG GGVCGRGRGR GRGRGRGRGR GRGRGRPQSG
110 120 130 140 150
GSGLGGDGGG GAGGCGGGSG GGVAPRRDPV PFPSGSSGPG PRGPRATESG
160 170 180 190 200
KRMDCPALPP GWKKEEVIRK SGLSAGKSDV YYFSPSGKKF RSKPQLARYL
210 220 230 240 250
GNAVDLSSFD FRTGKMMPSK LQKNKQRLRN DPLNQNKGKP DLNTTLPIRQ
260 270 280 290 300
TASIFKQPVT KFTNHPSNKV KSDPQRMNEQ PRQLFWEKRL QGLSASDVTE
310 320 330 340 350
QIIKTMELPK GLQGVGPGSN DETLLSAVAS ALHTSSAPIT GQVSAAVEKN
360 370 380 390 400
PAVWLNTSQP LCKAFIVTDE DIRKQEERVQ QVRKKLEEAL MADILSRAAD
410
TEEVDIDMDS GDEA
Length:414
Mass (Da):43,501
Last modified:July 27, 2011 - v2
Checksum:i1C658D4A6066602A
GO
Isoform 2 (identifier: Q9Z2E1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     238-249: GKPDLNTTLPIR → FRLIKKQTLIGL
     250-414: Missing.

Show »
Length:249
Mass (Da):25,451
Checksum:i530D600B81F98FF0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti117 – 1171G → V in AAC68872. (PubMed:9774669)Curated
Sequence conflicti117 – 1171G → V in AAC68874. (PubMed:9774669)Curated
Sequence conflicti117 – 1171G → V in AAD50372. (PubMed:10441743)Curated
Sequence conflicti117 – 1171G → V in AAD50373. (PubMed:10441743)Curated
Sequence conflicti117 – 1171G → V in AAH46607. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei238 – 24912GKPDL…TLPIR → FRLIKKQTLIGL in isoform 2. 1 PublicationVSP_011079Add
BLAST
Alternative sequencei250 – 414165Missing in isoform 2. 1 PublicationVSP_011080Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF072243 mRNA. Translation: AAC68872.1.
AF072245 mRNA. Translation: AAC68874.1.
AF120986
, AF120983, AF120984, AF120985 Genomic DNA. Translation: AAD50372.1.
AF120983 Genomic DNA. Translation: AAD50373.1.
AC134831 Genomic DNA. No translation available.
AC166815 Genomic DNA. No translation available.
AC170591 Genomic DNA. No translation available.
BC046607 mRNA. Translation: AAH46607.2.
CCDSiCCDS29335.1. [Q9Z2E1-1]
RefSeqiNP_034903.2. NM_010773.2. [Q9Z2E1-1]
XP_006525775.1. XM_006525712.1. [Q9Z2E1-2]
UniGeneiMm.322.

Genome annotation databases

EnsembliENSMUST00000074058; ENSMUSP00000073701; ENSMUSG00000024513. [Q9Z2E1-1]
ENSMUST00000114946; ENSMUSP00000110596; ENSMUSG00000024513. [Q9Z2E1-2]
GeneIDi17191.
KEGGimmu:17191.
UCSCiuc008fom.2. mouse. [Q9Z2E1-2]
uc008fon.2. mouse. [Q9Z2E1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF072243 mRNA. Translation: AAC68872.1 .
AF072245 mRNA. Translation: AAC68874.1 .
AF120986
, AF120983 , AF120984 , AF120985 Genomic DNA. Translation: AAD50372.1 .
AF120983 Genomic DNA. Translation: AAD50373.1 .
AC134831 Genomic DNA. No translation available.
AC166815 Genomic DNA. No translation available.
AC170591 Genomic DNA. No translation available.
BC046607 mRNA. Translation: AAH46607.2 .
CCDSi CCDS29335.1. [Q9Z2E1-1 ]
RefSeqi NP_034903.2. NM_010773.2. [Q9Z2E1-1 ]
XP_006525775.1. XM_006525712.1. [Q9Z2E1-2 ]
UniGenei Mm.322.

3D structure databases

ProteinModelPortali Q9Z2E1.
SMRi Q9Z2E1. Positions 152-216, 363-396.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201331. 6 interactions.
IntActi Q9Z2E1. 4 interactions.
MINTi MINT-4101332.

PTM databases

PhosphoSitei Q9Z2E1.

Proteomic databases

MaxQBi Q9Z2E1.
PaxDbi Q9Z2E1.
PRIDEi Q9Z2E1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000074058 ; ENSMUSP00000073701 ; ENSMUSG00000024513 . [Q9Z2E1-1 ]
ENSMUST00000114946 ; ENSMUSP00000110596 ; ENSMUSG00000024513 . [Q9Z2E1-2 ]
GeneIDi 17191.
KEGGi mmu:17191.
UCSCi uc008fom.2. mouse. [Q9Z2E1-2 ]
uc008fon.2. mouse. [Q9Z2E1-1 ]

Organism-specific databases

CTDi 8932.
MGIi MGI:1333813. Mbd2.

Phylogenomic databases

eggNOGi NOG316386.
GeneTreei ENSGT00410000025376.
HOGENOMi HOG000013073.
HOVERGENi HBG052417.
InParanoidi Q9Z2E1.
KOi K11590.
OMAi NCISICC.
OrthoDBi EOG7QNVMV.
TreeFami TF325032.

Enzyme and pathway databases

Reactomei REACT_214440. NoRC negatively regulates rRNA expression.

Miscellaneous databases

ChiTaRSi MBD2. mouse.
NextBioi 291534.
PROi Q9Z2E1.
SOURCEi Search...

Gene expression databases

Bgeei Q9Z2E1.
CleanExi MM_MBD2.
ExpressionAtlasi Q9Z2E1. baseline and differential.
Genevestigatori Q9Z2E1.

Family and domain databases

Gene3Di 3.30.890.10. 2 hits.
InterProi IPR016177. DNA-bd_dom.
IPR025884. MeCpG-bd_2/3_C_dom.
IPR001739. Methyl_CpG_DNA-bd.
[Graphical view ]
Pfami PF01429. MBD. 1 hit.
PF14048. MBD_C. 1 hit.
[Graphical view ]
SMARTi SM00391. MBD. 1 hit.
[Graphical view ]
SUPFAMi SSF54171. SSF54171. 1 hit.
PROSITEi PS50982. MBD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a family of mammalian methyl-CpG binding proteins."
    Hendrich B., Bird A.
    Mol. Cell. Biol. 18:6538-6547(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: C57BL/6.
  2. "Genomic structure and chromosomal mapping of the murine and human mbd1, mbd2, mbd3, and mbd4 genes."
    Hendrich B., Abbott C., McQueen H., Chambers D., Cross S.H., Bird A.
    Mamm. Genome 10:906-912(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Colon.
  5. "Role of human ribosomal RNA (rRNA) promoter methylation and of methyl-CpG-binding protein MBD2 in the suppression of rRNA gene expression."
    Ghoshal K., Majumder S., Datta J., Motiwala T., Bai S., Sharma S.M., Frankel W., Jacob S.T.
    J. Biol. Chem. 279:6783-6793(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMBD2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2E1
Secondary accession number(s): E9QMV9, Q811D9, Q9Z2D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3