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Q9Z2E1

- MBD2_MOUSE

UniProt

Q9Z2E1 - MBD2_MOUSE

Protein

Methyl-CpG-binding domain protein 2

Gene

Mbd2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression By similarity. May enhance the activation of some unmethylated cAMP-responsive promoters By similarity. Selectively represses transcription activity of methylated rRNA promoters.By similarity2 Publications

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: MGI
    3. methyl-CpG binding Source: MGI
    4. mRNA binding Source: MGI
    5. siRNA binding Source: MGI

    GO - Biological processi

    1. cellular protein complex assembly Source: MGI
    2. maternal behavior Source: MGI
    3. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    4. positive regulation of Wnt signaling pathway Source: MGI
    5. regulation of cell proliferation Source: MGI
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_214440. NoRC negatively regulates rRNA expression.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methyl-CpG-binding domain protein 2
    Alternative name(s):
    Methyl-CpG-binding protein MBD2
    Gene namesi
    Name:Mbd2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:1333813. Mbd2.

    Subcellular locationi

    Nucleus By similarity
    Note: Nuclear, in discrete foci. Detected at replication foci in late S phase By similarity.By similarity

    GO - Cellular componenti

    1. chromatin Source: MGI
    2. cytoplasm Source: MGI
    3. heterochromatin Source: MGI
    4. histone deacetylase complex Source: MGI
    5. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 414414Methyl-CpG-binding domain protein 2PRO_0000096261Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei184 – 1841PhosphoserineBy similarity
    Modified residuei410 – 4101PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9Z2E1.
    PaxDbiQ9Z2E1.
    PRIDEiQ9Z2E1.

    PTM databases

    PhosphoSiteiQ9Z2E1.

    Expressioni

    Tissue specificityi

    Highly expressed in brain, heart, kidney, lung, skeletal muscle, spleen and testis. Detected at lower levels in embryonic stem cells.1 Publication

    Gene expression databases

    ArrayExpressiQ9Z2E1.
    BgeeiQ9Z2E1.
    CleanExiMM_MBD2.
    GenevestigatoriQ9Z2E1.

    Interactioni

    Subunit structurei

    Heterodimer with MBD3. Component of the MeCP1 complex that contains HDAC1 and HDAC2. Binds DNMT1, MIZF, GPN1, SIN3A, GATAD2A/p66-alpha and GATAD2B/p66-beta By similarity. Interacts with DHX9 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi201331. 6 interactions.
    IntActiQ9Z2E1. 4 interactions.
    MINTiMINT-4101332.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z2E1.
    SMRiQ9Z2E1. Positions 152-216, 363-396.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini148 – 21669MBDPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi6 – 143138Gly-richAdd
    BLAST
    Compositional biasi50 – 9647Arg-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 MBD (methyl-CpG-binding) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG316386.
    GeneTreeiENSGT00410000025376.
    HOGENOMiHOG000013073.
    HOVERGENiHBG052417.
    InParanoidiQ9Z2E1.
    KOiK11590.
    OMAiNCISICC.
    OrthoDBiEOG7QNVMV.
    TreeFamiTF325032.

    Family and domain databases

    Gene3Di3.30.890.10. 2 hits.
    InterProiIPR016177. DNA-bd_dom.
    IPR025884. MeCpG-bd_2/3_C_dom.
    IPR001739. Methyl_CpG_DNA-bd.
    [Graphical view]
    PfamiPF01429. MBD. 1 hit.
    PF14048. MBD_C. 1 hit.
    [Graphical view]
    SMARTiSM00391. MBD. 1 hit.
    [Graphical view]
    SUPFAMiSSF54171. SSF54171. 1 hit.
    PROSITEiPS50982. MBD. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9Z2E1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRAHPGGGRC CPEQEEGESA AGGSGAGGDS AIEQGGQGSA LAPSPVSGVR    50
    REGARGGGRG RGRWKQAARG GGVCGRGRGR GRGRGRGRGR GRGRGRPQSG 100
    GSGLGGDGGG GAGGCGGGSG GGVAPRRDPV PFPSGSSGPG PRGPRATESG 150
    KRMDCPALPP GWKKEEVIRK SGLSAGKSDV YYFSPSGKKF RSKPQLARYL 200
    GNAVDLSSFD FRTGKMMPSK LQKNKQRLRN DPLNQNKGKP DLNTTLPIRQ 250
    TASIFKQPVT KFTNHPSNKV KSDPQRMNEQ PRQLFWEKRL QGLSASDVTE 300
    QIIKTMELPK GLQGVGPGSN DETLLSAVAS ALHTSSAPIT GQVSAAVEKN 350
    PAVWLNTSQP LCKAFIVTDE DIRKQEERVQ QVRKKLEEAL MADILSRAAD 400
    TEEVDIDMDS GDEA 414
    Length:414
    Mass (Da):43,501
    Last modified:July 27, 2011 - v2
    Checksum:i1C658D4A6066602A
    GO
    Isoform 2 (identifier: Q9Z2E1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         238-249: GKPDLNTTLPIR → FRLIKKQTLIGL
         250-414: Missing.

    Show »
    Length:249
    Mass (Da):25,451
    Checksum:i530D600B81F98FF0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti117 – 1171G → V in AAC68872. (PubMed:9774669)Curated
    Sequence conflicti117 – 1171G → V in AAC68874. (PubMed:9774669)Curated
    Sequence conflicti117 – 1171G → V in AAD50372. (PubMed:10441743)Curated
    Sequence conflicti117 – 1171G → V in AAD50373. (PubMed:10441743)Curated
    Sequence conflicti117 – 1171G → V in AAH46607. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei238 – 24912GKPDL…TLPIR → FRLIKKQTLIGL in isoform 2. 1 PublicationVSP_011079Add
    BLAST
    Alternative sequencei250 – 414165Missing in isoform 2. 1 PublicationVSP_011080Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF072243 mRNA. Translation: AAC68872.1.
    AF072245 mRNA. Translation: AAC68874.1.
    AF120986
    , AF120983, AF120984, AF120985 Genomic DNA. Translation: AAD50372.1.
    AF120983 Genomic DNA. Translation: AAD50373.1.
    AC134831 Genomic DNA. No translation available.
    AC166815 Genomic DNA. No translation available.
    AC170591 Genomic DNA. No translation available.
    BC046607 mRNA. Translation: AAH46607.2.
    CCDSiCCDS29335.1. [Q9Z2E1-1]
    RefSeqiNP_034903.2. NM_010773.2. [Q9Z2E1-1]
    XP_006525775.1. XM_006525712.1. [Q9Z2E1-2]
    UniGeneiMm.322.

    Genome annotation databases

    EnsembliENSMUST00000074058; ENSMUSP00000073701; ENSMUSG00000024513. [Q9Z2E1-1]
    ENSMUST00000114946; ENSMUSP00000110596; ENSMUSG00000024513. [Q9Z2E1-2]
    GeneIDi17191.
    KEGGimmu:17191.
    UCSCiuc008fom.2. mouse. [Q9Z2E1-2]
    uc008fon.2. mouse. [Q9Z2E1-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF072243 mRNA. Translation: AAC68872.1 .
    AF072245 mRNA. Translation: AAC68874.1 .
    AF120986
    , AF120983 , AF120984 , AF120985 Genomic DNA. Translation: AAD50372.1 .
    AF120983 Genomic DNA. Translation: AAD50373.1 .
    AC134831 Genomic DNA. No translation available.
    AC166815 Genomic DNA. No translation available.
    AC170591 Genomic DNA. No translation available.
    BC046607 mRNA. Translation: AAH46607.2 .
    CCDSi CCDS29335.1. [Q9Z2E1-1 ]
    RefSeqi NP_034903.2. NM_010773.2. [Q9Z2E1-1 ]
    XP_006525775.1. XM_006525712.1. [Q9Z2E1-2 ]
    UniGenei Mm.322.

    3D structure databases

    ProteinModelPortali Q9Z2E1.
    SMRi Q9Z2E1. Positions 152-216, 363-396.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201331. 6 interactions.
    IntActi Q9Z2E1. 4 interactions.
    MINTi MINT-4101332.

    PTM databases

    PhosphoSitei Q9Z2E1.

    Proteomic databases

    MaxQBi Q9Z2E1.
    PaxDbi Q9Z2E1.
    PRIDEi Q9Z2E1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000074058 ; ENSMUSP00000073701 ; ENSMUSG00000024513 . [Q9Z2E1-1 ]
    ENSMUST00000114946 ; ENSMUSP00000110596 ; ENSMUSG00000024513 . [Q9Z2E1-2 ]
    GeneIDi 17191.
    KEGGi mmu:17191.
    UCSCi uc008fom.2. mouse. [Q9Z2E1-2 ]
    uc008fon.2. mouse. [Q9Z2E1-1 ]

    Organism-specific databases

    CTDi 8932.
    MGIi MGI:1333813. Mbd2.

    Phylogenomic databases

    eggNOGi NOG316386.
    GeneTreei ENSGT00410000025376.
    HOGENOMi HOG000013073.
    HOVERGENi HBG052417.
    InParanoidi Q9Z2E1.
    KOi K11590.
    OMAi NCISICC.
    OrthoDBi EOG7QNVMV.
    TreeFami TF325032.

    Enzyme and pathway databases

    Reactomei REACT_214440. NoRC negatively regulates rRNA expression.

    Miscellaneous databases

    ChiTaRSi MBD2. mouse.
    NextBioi 291534.
    PROi Q9Z2E1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Z2E1.
    Bgeei Q9Z2E1.
    CleanExi MM_MBD2.
    Genevestigatori Q9Z2E1.

    Family and domain databases

    Gene3Di 3.30.890.10. 2 hits.
    InterProi IPR016177. DNA-bd_dom.
    IPR025884. MeCpG-bd_2/3_C_dom.
    IPR001739. Methyl_CpG_DNA-bd.
    [Graphical view ]
    Pfami PF01429. MBD. 1 hit.
    PF14048. MBD_C. 1 hit.
    [Graphical view ]
    SMARTi SM00391. MBD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54171. SSF54171. 1 hit.
    PROSITEi PS50982. MBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of a family of mammalian methyl-CpG binding proteins."
      Hendrich B., Bird A.
      Mol. Cell. Biol. 18:6538-6547(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: C57BL/6.
    2. "Genomic structure and chromosomal mapping of the murine and human mbd1, mbd2, mbd3, and mbd4 genes."
      Hendrich B., Abbott C., McQueen H., Chambers D., Cross S.H., Bird A.
      Mamm. Genome 10:906-912(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Colon.
    5. "Role of human ribosomal RNA (rRNA) promoter methylation and of methyl-CpG-binding protein MBD2 in the suppression of rRNA gene expression."
      Ghoshal K., Majumder S., Datta J., Motiwala T., Bai S., Sharma S.M., Frankel W., Jacob S.T.
      J. Biol. Chem. 279:6783-6793(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiMBD2_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z2E1
    Secondary accession number(s): E9QMV9, Q811D9, Q9Z2D9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3