Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9Z2E1 (MBD2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methyl-CpG-binding domain protein 2
Alternative name(s):
Methyl-CpG-binding protein MBD2
Gene names
Name:Mbd2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length414 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression By similarity. May enhance the activation of some unmethylated cAMP-responsive promoters By similarity. Selectively represses transcription activity of methylated rRNA promoters. Ref.1 Ref.5

Subunit structure

Heterodimer with MBD3. Component of the MeCP1 complex that contains HDAC1 and HDAC2. Binds DNMT1, MIZF, GPN1, SIN3A, GATAD2A/p66-alpha and GATAD2B/p66-beta By similarity. Interacts with DHX9 By similarity.

Subcellular location

Nucleus By similarity. Note: Nuclear, in discrete foci. Detected at replication foci in late S phase By similarity. Ref.1 Ref.5

Tissue specificity

Highly expressed in brain, heart, kidney, lung, skeletal muscle, spleen and testis. Detected at lower levels in embryonic stem cells. Ref.1

Sequence similarities

Contains 1 MBD (methyl-CpG-binding) domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular protein complex assembly

Inferred from mutant phenotype PubMed 11274056. Source: MGI

maternal behavior

Inferred from mutant phenotype PubMed 11274056. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 11242117PubMed 11274056PubMed 18644872. Source: MGI

positive regulation of Wnt signaling pathway

Inferred from genetic interaction PubMed 18644872. Source: MGI

regulation of cell proliferation

Inferred from genetic interaction PubMed 18644872. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentchromatin

Inferred from direct assay Ref.1. Source: MGI

cytoplasm

Inferred from direct assay PubMed 17546630. Source: MGI

heterochromatin

Inferred from direct assay PubMed 14519686PubMed 14643676. Source: MGI

histone deacetylase complex

Inferred from direct assay PubMed 11274056. Source: MGI

nucleus

Inferred from direct assay PubMed 11274056PubMed 17546630Ref.1. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay Ref.1. Source: MGI

chromatin binding

Inferred from direct assay PubMed 16608912PubMed 18644872. Source: MGI

mRNA binding

Inferred from direct assay PubMed 15342650. Source: MGI

methyl-CpG binding

Inferred from direct assay PubMed 15342650. Source: MGI

siRNA binding

Inferred from direct assay PubMed 15342650. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Z2E1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Z2E1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     238-249: GKPDLNTTLPIR → FRLIKKQTLIGL
     250-414: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 414414Methyl-CpG-binding domain protein 2
PRO_0000096261

Regions

Domain148 – 21669MBD
Compositional bias6 – 143138Gly-rich
Compositional bias50 – 9647Arg-rich

Amino acid modifications

Modified residue1841Phosphoserine By similarity
Modified residue4101Phosphoserine By similarity

Natural variations

Alternative sequence238 – 24912GKPDL…TLPIR → FRLIKKQTLIGL in isoform 2.
VSP_011079
Alternative sequence250 – 414165Missing in isoform 2.
VSP_011080

Experimental info

Sequence conflict1171G → V in AAC68872. Ref.1
Sequence conflict1171G → V in AAC68874. Ref.1
Sequence conflict1171G → V in AAD50372. Ref.2
Sequence conflict1171G → V in AAD50373. Ref.2
Sequence conflict1171G → V in AAH46607. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 1C658D4A6066602A

FASTA41443,501
        10         20         30         40         50         60 
MRAHPGGGRC CPEQEEGESA AGGSGAGGDS AIEQGGQGSA LAPSPVSGVR REGARGGGRG 

        70         80         90        100        110        120 
RGRWKQAARG GGVCGRGRGR GRGRGRGRGR GRGRGRPQSG GSGLGGDGGG GAGGCGGGSG 

       130        140        150        160        170        180 
GGVAPRRDPV PFPSGSSGPG PRGPRATESG KRMDCPALPP GWKKEEVIRK SGLSAGKSDV 

       190        200        210        220        230        240 
YYFSPSGKKF RSKPQLARYL GNAVDLSSFD FRTGKMMPSK LQKNKQRLRN DPLNQNKGKP 

       250        260        270        280        290        300 
DLNTTLPIRQ TASIFKQPVT KFTNHPSNKV KSDPQRMNEQ PRQLFWEKRL QGLSASDVTE 

       310        320        330        340        350        360 
QIIKTMELPK GLQGVGPGSN DETLLSAVAS ALHTSSAPIT GQVSAAVEKN PAVWLNTSQP 

       370        380        390        400        410 
LCKAFIVTDE DIRKQEERVQ QVRKKLEEAL MADILSRAAD TEEVDIDMDS GDEA 

« Hide

Isoform 2 [UniParc].

Checksum: 530D600B81F98FF0
Show »

FASTA24925,451

References

« Hide 'large scale' references
[1]"Identification and characterization of a family of mammalian methyl-CpG binding proteins."
Hendrich B., Bird A.
Mol. Cell. Biol. 18:6538-6547(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
Strain: C57BL/6.
[2]"Genomic structure and chromosomal mapping of the murine and human mbd1, mbd2, mbd3, and mbd4 genes."
Hendrich B., Abbott C., McQueen H., Chambers D., Cross S.H., Bird A.
Mamm. Genome 10:906-912(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Colon.
[5]"Role of human ribosomal RNA (rRNA) promoter methylation and of methyl-CpG-binding protein MBD2 in the suppression of rRNA gene expression."
Ghoshal K., Majumder S., Datta J., Motiwala T., Bai S., Sharma S.M., Frankel W., Jacob S.T.
J. Biol. Chem. 279:6783-6793(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF072243 mRNA. Translation: AAC68872.1.
AF072245 mRNA. Translation: AAC68874.1.
AF120986 expand/collapse EMBL AC list , AF120983, AF120984, AF120985 Genomic DNA. Translation: AAD50372.1.
AF120983 Genomic DNA. Translation: AAD50373.1.
AC134831 Genomic DNA. No translation available.
AC166815 Genomic DNA. No translation available.
AC170591 Genomic DNA. No translation available.
BC046607 mRNA. Translation: AAH46607.2.
CCDSCCDS29335.1. [Q9Z2E1-1]
RefSeqNP_034903.2. NM_010773.2. [Q9Z2E1-1]
XP_006525775.1. XM_006525712.1. [Q9Z2E1-2]
UniGeneMm.322.

3D structure databases

ProteinModelPortalQ9Z2E1.
SMRQ9Z2E1. Positions 152-216, 363-396.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201331. 6 interactions.
IntActQ9Z2E1. 4 interactions.
MINTMINT-4101332.

PTM databases

PhosphoSiteQ9Z2E1.

Proteomic databases

MaxQBQ9Z2E1.
PaxDbQ9Z2E1.
PRIDEQ9Z2E1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000074058; ENSMUSP00000073701; ENSMUSG00000024513. [Q9Z2E1-1]
ENSMUST00000114946; ENSMUSP00000110596; ENSMUSG00000024513. [Q9Z2E1-2]
GeneID17191.
KEGGmmu:17191.
UCSCuc008fom.2. mouse. [Q9Z2E1-2]
uc008fon.2. mouse. [Q9Z2E1-1]

Organism-specific databases

CTD8932.
MGIMGI:1333813. Mbd2.

Phylogenomic databases

eggNOGNOG316386.
GeneTreeENSGT00410000025376.
HOGENOMHOG000013073.
HOVERGENHBG052417.
InParanoidQ9Z2E1.
KOK11590.
OMANCISICC.
OrthoDBEOG7QNVMV.
TreeFamTF325032.

Gene expression databases

ArrayExpressQ9Z2E1.
BgeeQ9Z2E1.
CleanExMM_MBD2.
GenevestigatorQ9Z2E1.

Family and domain databases

Gene3D3.30.890.10. 2 hits.
InterProIPR016177. DNA-bd_dom.
IPR025884. MeCpG-bd_2/3_C_dom.
IPR001739. Methyl_CpG_DNA-bd.
[Graphical view]
PfamPF01429. MBD. 1 hit.
PF14048. MBD_C. 1 hit.
[Graphical view]
SMARTSM00391. MBD. 1 hit.
[Graphical view]
SUPFAMSSF54171. SSF54171. 1 hit.
PROSITEPS50982. MBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMBD2. mouse.
NextBio291534.
PROQ9Z2E1.
SOURCESearch...

Entry information

Entry nameMBD2_MOUSE
AccessionPrimary (citable) accession number: Q9Z2E1
Secondary accession number(s): E9QMV9, Q811D9, Q9Z2D9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot