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Protein

Methyl-CpG-binding domain protein 3

Gene

Mbd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as transcriptional repressor and plays a role in gene silencing. Does not bind DNA by itself. Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (By similarity). Recruits histone deacetylases and DNA methyltransferases.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • aging Source: Ensembl
  • ATP-dependent chromatin remodeling Source: MGI
  • brain development Source: Ensembl
  • embryonic organ development Source: Ensembl
  • heart development Source: Ensembl
  • histone acetylation Source: MGI
  • in utero embryonic development Source: MGI
  • methylation-dependent chromatin silencing Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of DNA methylation Source: Ensembl
  • response to estradiol Source: Ensembl
  • response to nutrient levels Source: Ensembl
  • tissue development Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-6804758. Regulation of TP53 Activity through Acetylation.
R-MMU-73762. RNA Polymerase I Transcription Initiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Methyl-CpG-binding domain protein 3
Alternative name(s):
Methyl-CpG-binding protein MBD3
Gene namesi
Name:Mbd3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1333812. Mbd3.

Subcellular locationi

GO - Cellular componenti

  • chromatin Source: MGI
  • cytoplasm Source: MGI
  • heterochromatin Source: MGI
  • nuclear chromatin Source: MGI
  • nucleoplasm Source: Reactome
  • nucleus Source: MGI
  • NuRD complex Source: MGI
  • protein complex Source: MGI

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000962631 – 285Methyl-CpG-binding domain protein 3Add BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei56PhosphoserineCombined sources1
Cross-linki73Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei85PhosphoserineBy similarity1
Cross-linki90Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki92Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei144PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9Z2D8.
PeptideAtlasiQ9Z2D8.
PRIDEiQ9Z2D8.

PTM databases

iPTMnetiQ9Z2D8.
PhosphoSitePlusiQ9Z2D8.

Expressioni

Tissue specificityi

Highly expressed in brain, heart, kidney, liver, lung, skeletal muscle, spleen and testis. Detected at lower levels in embryonic stem cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000035478.
CleanExiMM_MBD3.
ExpressionAtlasiQ9Z2D8. baseline and differential.
GenevisibleiQ9Z2D8. MM.

Interactioni

Subunit structurei

Heterodimer with MBD2. Part of the NuRD and the MeCP1 complex. Interacts with BCL6, HDAC1, MTA2, DNMT1, p66-alpha and p66-beta (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
MecomP144045EBI-1994598,EBI-1994523

Protein-protein interaction databases

BioGridi201332. 15 interactors.
DIPiDIP-46518N.
IntActiQ9Z2D8. 11 interactors.
MINTiMINT-4101368.
STRINGi10090.ENSMUSP00000089948.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2D8.
SMRiQ9Z2D8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 69MBDPROSITE-ProRule annotationAdd BLAST69

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili221 – 279Sequence analysisAdd BLAST59

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi229 – 283Glu-richAdd BLAST55

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4161. Eukaryota.
ENOG4111HPQ. LUCA.
GeneTreeiENSGT00410000025376.
HOGENOMiHOG000013073.
HOVERGENiHBG052417.
InParanoidiQ9Z2D8.
KOiK11591.
OMAiLMHAVII.
OrthoDBiEOG091G0I05.
PhylomeDBiQ9Z2D8.
TreeFamiTF325032.

Family and domain databases

InterProiView protein in InterPro
IPR016177. DNA-bd_dom.
IPR032343. MBD2/MBD3_p55-bd.
IPR025884. MeCpG-bd_2/3_C_dom.
IPR001739. Methyl_CpG_DNA-bd.
PfamiView protein in Pfam
PF01429. MBD. 1 hit.
PF14048. MBD_C. 1 hit.
PF16564. MBDa. 1 hit.
SMARTiView protein in SMART
SM00391. MBD. 1 hit.
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiView protein in PROSITE
PS50982. MBD. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z2D8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERKRWECPA LPQGWEREEV PRRSGLSAGH RDVFYYSPSG KKFRSKPQLA
60 70 80 90 100
RYLGGSMDLS TFDFRTGKML MNKMNKSRQR VRYDSSNQVK GKPDLNTALP
110 120 130 140 150
VRQTASIFKQ PVTKITNHPS NKVKSDPQKA VDQPRQLFWE KKLSGLSAFD
160 170 180 190 200
IAEELVRTMD LPKGLQGVGP GCTDETLLSA IASALHTSTL PITGQLSAAV
210 220 230 240 250
EKNPGVWLNT AQPLCKAFMV TDDDIRKQEE LVQQVRKRLE EALMADMLAH
260 270 280
VEELARDGEA PLDKACAEEE EEEEEEEEEP EPERV
Length:285
Mass (Da):32,168
Last modified:May 1, 1999 - v1
Checksum:iE4E57BD48463643F
GO
Isoform 2 (identifier: Q9Z2D8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     5-36: Missing.

Note: No experimental confirmation available.
Show »
Length:253
Mass (Da):28,338
Checksum:iAD85860066812B03
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0110825 – 36Missing in isoform 2. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF072248 mRNA. Translation: AAC68877.1.
AF120995 Genomic DNA. Translation: AAD48909.1.
BC038264 mRNA. Translation: AAH38264.1.
CCDSiCCDS24020.1. [Q9Z2D8-1]
CCDS78860.1. [Q9Z2D8-2]
RefSeqiNP_001293072.1. NM_001306143.1. [Q9Z2D8-2]
NP_038623.1. NM_013595.3. [Q9Z2D8-1]
UniGeneiMm.7142.

Genome annotation databases

EnsembliENSMUST00000092295; ENSMUSP00000089948; ENSMUSG00000035478. [Q9Z2D8-1]
ENSMUST00000105349; ENSMUSP00000100986; ENSMUSG00000035478. [Q9Z2D8-2]
GeneIDi17192.
KEGGimmu:17192.
UCSCiuc007gda.1. mouse. [Q9Z2D8-2]
uc007gdb.1. mouse. [Q9Z2D8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMBD3_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2D8
Secondary accession number(s): Q792D3, Q8CFJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 1, 1999
Last modified: August 30, 2017
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot