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Q9Z2D7 (MBD4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methyl-CpG-binding domain protein 4

EC=3.2.2.-
Alternative name(s):
Methyl-CpG-binding protein MBD4
Mismatch-specific DNA N-glycosylase
Gene names
Name:Mbd4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. Ref.1

Subunit structure

Interacts with MLH1 By similarity.

Subcellular location

Nucleus. Note: Nuclear, in discrete foci. Ref.1

Sequence similarities

Contains 1 MBD (methyl-CpG-binding) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 554554Methyl-CpG-binding domain protein 4
PRO_0000096265

Regions

Domain63 – 13573MBD

Sites

Active site5341 By similarity

Experimental info

Sequence conflict1291N → D in AAH24812. Ref.3

Secondary structure

.................................. 554
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9Z2D7 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 792D37CB180291F5

FASTA55462,578
        10         20         30         40         50         60 
MESPNLGDNR VRGESLVPDP PWDRCKEDIA VGLGGVGEDG KDLVISSERS SLLQEPTAST 

        70         80         90        100        110        120 
LSSTTATEGH KPVPCGWERV VKQRLSGKTA GKFDVYFISP QGLKFRSKRS LANYLLKNGE 

       130        140        150        160        170        180 
TFLKPEDFNF TVLPKGSINP GYKHQSLAAL TSLQPNETDV SKQNLKTRSK WKTDVLPLPS 

       190        200        210        220        230        240 
GTSESPESSG LSNSNSACLL LREHRDIQDV DSEKRRKSKR KVTVLKGTAS QKTKQKCRKS 

       250        260        270        280        290        300 
LLESTQRNRK RASVVQKVGA DRELVPQESQ LNRTLCPADA CARETVGLAG EEKSPSPGLD 

       310        320        330        340        350        360 
LCFIQVTSGT TNKFHSTEAA GEANREQTFL ESEEIRSKGD RKGEAHLHTG VLQDGSEMPS 

       370        380        390        400        410        420 
CSQAKKHFTS ETFQEDSIPR TQVEKRKTSL YFSSKYNKEA LSPPRRKSFK KWTPPRSPFN 

       430        440        450        460        470        480 
LVQEILFHDP WKLLIATIFL NRTSGKMAIP VLWEFLEKYP SAEVARAADW RDVSELLKPL 

       490        500        510        520        530        540 
GLYDLRAKTI IKFSDEYLTK QWRYPIELHG IGKYGNDSYR IFCVNEWKQV HPEDHKLNKY 

       550 
HDWLWENHEK LSLS 

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of a family of mammalian methyl-CpG binding proteins."
Hendrich B., Bird A.
Mol. Cell. Biol. 18:6538-6547(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, FUNCTION, SUBCELLULAR LOCATION.
[2]"Genomic structure and chromosomal mapping of the murine and human mbd1, mbd2, mbd3, and mbd4 genes."
Hendrich B., Abbott C., McQueen H., Chambers D., Cross S.H., Bird A.
Mamm. Genome 10:906-912(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 129.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Mismatch repair in methylated DNA. Structure and activity of the mismatch-specific thymine glycosylase domain of methyl-CpG-binding protein MBD4."
Wu P., Qiu C., Sohail A., Zhang X., Bhagwat A.S., Cheng X.
J. Biol. Chem. 278:5285-5291(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 411-554.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF072249 mRNA. Translation: AAC68878.1.
AF120996 Genomic DNA. Translation: AAD56595.1.
BC024812 mRNA. Translation: AAH24812.1.
CCDSCCDS39603.1.
RefSeqNP_034904.2. NM_010774.2.
UniGeneMm.259308.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1NGNX-ray2.10A400-554[»]
3VXVX-ray2.00A69-136[»]
3VXXX-ray2.20A69-136[»]
3VYBX-ray2.40A69-136[»]
3VYQX-ray2.52A/D63-136[»]
4EVVX-ray2.39A411-554[»]
4EW0X-ray2.39A411-554[»]
4EW4X-ray2.79A411-554[»]
ProteinModelPortalQ9Z2D7.
SMRQ9Z2D7. Positions 71-135, 411-554.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9Z2D7.

Proteomic databases

PaxDbQ9Z2D7.
PRIDEQ9Z2D7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032469; ENSMUSP00000032469; ENSMUSG00000030322.
GeneID17193.
KEGGmmu:17193.
UCSCuc009dje.2. mouse.

Organism-specific databases

CTD8930.
MGIMGI:1333850. Mbd4.

Phylogenomic databases

eggNOGNOG264445.
GeneTreeENSGT00530000063687.
HOGENOMHOG000113489.
HOVERGENHBG052418.
InParanoidQ9Z2D7.
KOK10801.
OrthoDBEOG74N5J9.
PhylomeDBQ9Z2D7.
TreeFamTF329176.

Gene expression databases

ArrayExpressQ9Z2D7.
BgeeQ9Z2D7.
CleanExMM_MBD4.
GenevestigatorQ9Z2D7.

Family and domain databases

Gene3D1.10.340.30. 1 hit.
3.30.890.10. 1 hit.
InterProIPR016177. DNA-bd_dom.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR017352. Me_CpG-bd_MBD4.
IPR001739. Methyl_CpG_DNA-bd.
[Graphical view]
PfamPF00730. HhH-GPD. 1 hit.
PF01429. MBD. 1 hit.
[Graphical view]
PIRSFPIRSF038005. Methyl_CpG_bd_MBD4. 1 hit.
SMARTSM00391. MBD. 1 hit.
[Graphical view]
SUPFAMSSF48150. SSF48150. 1 hit.
SSF54171. SSF54171. 1 hit.
PROSITEPS50982. MBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9Z2D7.
NextBio291542.
PROQ9Z2D7.
SOURCESearch...

Entry information

Entry nameMBD4_MOUSE
AccessionPrimary (citable) accession number: Q9Z2D7
Secondary accession number(s): Q792D2, Q8R3R3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot