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Protein

Myotubularin-related protein 2

Gene

Mtmr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatase that acts on lipids with a phosphoinositol headgroup. Has phosphatase activity towards phosphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate. Binds phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate, phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate.1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O = 1-phosphatidyl-1D-myo-inositol + phosphate.1 Publication
1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate.1 Publication

Enzyme regulationi

Interaction with SBF1 increases phosphatase activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei417Phosphocysteine intermediate1
Binding sitei463SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • dendritic spine maintenance Source: Ensembl
  • dephosphorylation Source: MGI
  • inositol phosphate dephosphorylation Source: MGI
  • myelin assembly Source: MGI
  • negative regulation of excitatory postsynaptic potential Source: Ensembl
  • negative regulation of myelination Source: MGI
  • negative regulation of receptor catabolic process Source: Ensembl
  • negative regulation of receptor internalization Source: Ensembl
  • neuron development Source: MGI
  • phosphatidylinositol dephosphorylation Source: MGI
  • phosphatidylinositol metabolic process Source: MGI
  • positive regulation of early endosome to late endosome transport Source: Ensembl
  • protein tetramerization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.3.95. 3474.
ReactomeiR-MMU-1660516. Synthesis of PIPs at the early endosome membrane.
R-MMU-1660517. Synthesis of PIPs at the late endosome membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Myotubularin-related protein 2
Alternative name(s):
Phosphatidylinositol-3,5-bisphosphate 3-phosphatase (EC:3.1.3.951 Publication)
Phosphatidylinositol-3-phosphate phosphatase (EC:3.1.3.641 Publication)
Gene namesi
Name:Mtmr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1924366. Mtmr2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Early endosome membrane By similarity; Peripheral membrane protein By similarity

  • Note: Partly associated with membranes.By similarity

GO - Cellular componenti

  • axon Source: Ensembl
  • cytoplasm Source: MGI
  • cytosol Source: UniProtKB
  • dendritic spine Source: Ensembl
  • early endosome membrane Source: UniProtKB-SubCell
  • extracellular exosome Source: MGI
  • nucleus Source: MGI
  • synaptic membrane Source: Ensembl
  • synaptic vesicle Source: Ensembl
  • vacuolar membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi103G → E: Severely impaired interaction with membranes and strongly reduced catalytic activity. 2 Publications1
Mutagenesisi417C → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000949351 – 643Myotubularin-related protein 2Add BLAST643

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphoserineCombined sources1
Modified residuei9PhosphoserineCombined sources1
Modified residuei58PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-58 decreases MTMR2 localization to endocytic vesicular structures.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Z2D1.
MaxQBiQ9Z2D1.
PaxDbiQ9Z2D1.
PeptideAtlasiQ9Z2D1.
PRIDEiQ9Z2D1.

PTM databases

iPTMnetiQ9Z2D1.
PhosphoSitePlusiQ9Z2D1.

Expressioni

Tissue specificityi

Detected in fetal forebrain, dorsal root ganglia, trigeminal ganglia, kidney, adrenal gland and lung. Detected in adult dorsal root ganglia, neurons of the central nervous system, motor neurons, cell soma and neurites of sensory neurons, olfactory bulb, cerebellum and hippocampus.1 Publication

Gene expression databases

BgeeiENSMUSG00000031918.
CleanExiMM_MTMR2.
ExpressionAtlasiQ9Z2D1. baseline and differential.
GenevisibleiQ9Z2D1. MM.

Interactioni

Subunit structurei

Homooligomer and heterooligomer. Interacts with SBF1 and SBF2 (By similarity).By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

BioGridi218525. 7 interactors.
IntActiQ9Z2D1. 5 interactors.
STRINGi10090.ENSMUSP00000034396.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2D1.
SMRiQ9Z2D1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 139GRAMAdd BLAST72
Domaini205 – 580Myotubularin phosphatasePROSITE-ProRule annotationAdd BLAST376

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni330 – 333Substrate bindingBy similarity4
Regioni355 – 356Substrate bindingBy similarity2
Regioni417 – 423Substrate bindingBy similarity7

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili593 – 627By similarityAdd BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi4 – 53Ser-richAdd BLAST50

Sequence similaritiesi

Contains 1 GRAM domain.Curated
Contains 1 myotubularin phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1089. Eukaryota.
ENOG410XPTU. LUCA.
GeneTreeiENSGT00760000118832.
HOGENOMiHOG000210598.
HOVERGENiHBG000220.
InParanoidiQ9Z2D1.
KOiK18081.
OMAiNDMWRAT.
OrthoDBiEOG091G04DS.
TreeFamiTF315197.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR004182. GRAM.
IPR010569. Myotubularin-like_Pase_dom.
IPR030564. Myotubularin_fam.
IPR011993. PH_dom-like.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PANTHERiPTHR10807. PTHR10807. 1 hit.
PfamiPF02893. GRAM. 1 hit.
PF06602. Myotub-related. 1 hit.
[Graphical view]
SMARTiSM00568. GRAM. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF52799. SSF52799. 1 hit.
PROSITEiPS51339. PPASE_MYOTUBULARIN. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z2D1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKSSSCESL GAQLPAARLP SEDSLSSAST SHSENSVHTK SASAISSDSI
60 70 80 90 100
STSADNFSPD LRVLREANKL AEMEEPALLP GENIKDMAKD VTYICPFTGA
110 120 130 140 150
VRGTLTVTSY RLYFKSMERD PPFVLDASLG VISRVEKIGG ASSRGENSYG
160 170 180 190 200
LETVCKDIRN LRFAHKPEGR TRRSIFENLM KYAFPVSNGL PLFAFEYKEV
210 220 230 240 250
FPENGWKLYD PLLEYRRQGI PNESWRITKI NERYELCDTY PALLVVPANI
260 270 280 290 300
PDEELKRVAS FRSRGRIPVL SWIHPESQAT VTRCSQPMVG VSGKRSKEDE
310 320 330 340 350
KYLQAIMDSN AQSHKIFIFD ARPSVNAVAN KAKGGGYESE DAYQNAELVF
360 370 380 390 400
LDIHNIHVMR ESLRKLKEIV YPTIEETHWL SNLESTHWLE HIKLILAGAL
410 420 430 440 450
RIADKVESGK TSVVVHCSDG WDRTAQLTSL AMLMLDGYYR TIRGFEVLVE
460 470 480 490 500
KEWLSFGHRF QLRVGHGDKN HADADRSPVF LQFIDCVWQM TRQFPTAFEF
510 520 530 540 550
NEYFLITILD HLYSCLFGTF LCNSEQQRGK ENLPKKTVSL WSYINSQLED
560 570 580 590 600
FTNPLYGSYS NHVLYPVASM RHLELWVGYY IRWNPRMKPQ EPIHSRYKEL
610 620 630 640
LAKRAELQRK VEELQREISN RSTSSSERAS SPAQCVTPVQ TVV
Length:643
Mass (Da):73,232
Last modified:July 27, 2011 - v3
Checksum:iFD4E43867CBB9864
GO

Sequence cautioni

The sequence AAC80002 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY055832 mRNA. Translation: AAL14198.1.
CT010488 Genomic DNA. Translation: CAX15666.1.
CH466522 Genomic DNA. Translation: EDL24975.1.
AF073880 mRNA. Translation: AAC80002.1. Different initiation.
CCDSiCCDS22818.3.
RefSeqiNP_076347.3. NM_023858.3.
UniGeneiMm.210405.

Genome annotation databases

EnsembliENSMUST00000034396; ENSMUSP00000034396; ENSMUSG00000031918.
GeneIDi77116.
KEGGimmu:77116.
UCSCiuc009odz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY055832 mRNA. Translation: AAL14198.1.
CT010488 Genomic DNA. Translation: CAX15666.1.
CH466522 Genomic DNA. Translation: EDL24975.1.
AF073880 mRNA. Translation: AAC80002.1. Different initiation.
CCDSiCCDS22818.3.
RefSeqiNP_076347.3. NM_023858.3.
UniGeneiMm.210405.

3D structure databases

ProteinModelPortaliQ9Z2D1.
SMRiQ9Z2D1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218525. 7 interactors.
IntActiQ9Z2D1. 5 interactors.
STRINGi10090.ENSMUSP00000034396.

PTM databases

iPTMnetiQ9Z2D1.
PhosphoSitePlusiQ9Z2D1.

Proteomic databases

EPDiQ9Z2D1.
MaxQBiQ9Z2D1.
PaxDbiQ9Z2D1.
PeptideAtlasiQ9Z2D1.
PRIDEiQ9Z2D1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034396; ENSMUSP00000034396; ENSMUSG00000031918.
GeneIDi77116.
KEGGimmu:77116.
UCSCiuc009odz.2. mouse.

Organism-specific databases

CTDi8898.
MGIiMGI:1924366. Mtmr2.

Phylogenomic databases

eggNOGiKOG1089. Eukaryota.
ENOG410XPTU. LUCA.
GeneTreeiENSGT00760000118832.
HOGENOMiHOG000210598.
HOVERGENiHBG000220.
InParanoidiQ9Z2D1.
KOiK18081.
OMAiNDMWRAT.
OrthoDBiEOG091G04DS.
TreeFamiTF315197.

Enzyme and pathway databases

BRENDAi3.1.3.95. 3474.
ReactomeiR-MMU-1660516. Synthesis of PIPs at the early endosome membrane.
R-MMU-1660517. Synthesis of PIPs at the late endosome membrane.

Miscellaneous databases

PROiQ9Z2D1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031918.
CleanExiMM_MTMR2.
ExpressionAtlasiQ9Z2D1. baseline and differential.
GenevisibleiQ9Z2D1. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR004182. GRAM.
IPR010569. Myotubularin-like_Pase_dom.
IPR030564. Myotubularin_fam.
IPR011993. PH_dom-like.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PANTHERiPTHR10807. PTHR10807. 1 hit.
PfamiPF02893. GRAM. 1 hit.
PF06602. Myotub-related. 1 hit.
[Graphical view]
SMARTiSM00568. GRAM. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF52799. SSF52799. 1 hit.
PROSITEiPS51339. PPASE_MYOTUBULARIN. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTMR2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2D1
Secondary accession number(s): B8JJF4, Q8VHA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.