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Protein

Gamma-glutamyltransferase 5

Gene

Ggt5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cleaves the gamma-glutamyl peptide bond of glutathione conjugates, but maybe not glutathione itself. Converts leukotriene C4 (LTC4) to leukotriene D4 (LTD4).

Catalytic activityi

A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid.
Glutathione + H2O = L-cysteinylglycine + L-glutamate.
Leukotriene C4 + H2O = leukotriene D4 + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101GlutamateBy similarity
Active sitei389 – 3891NucleophileBy similarity
Binding sitei407 – 4071GlutamateBy similarity
Binding sitei428 – 4281GlutamateBy similarity

GO - Molecular functioni

  1. gamma-glutamyltransferase activity Source: MGI
  2. glutathione hydrolase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutathione biosynthetic process Source: UniProtKB-KW
  2. inflammatory response Source: MGI
  3. leukotriene biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Hydrolase, Protease, Transferase

Keywords - Biological processi

Glutathione biosynthesis, Leukotriene biosynthesis

Enzyme and pathway databases

ReactomeiREACT_215561. Synthesis of Leukotrienes (LT) and Eoxins (EX).
SABIO-RKQ9Z2A9.
UniPathwayiUPA00204.
UPA00880.

Protein family/group databases

MEROPSiT03.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-glutamyltransferase 5 (EC:2.3.2.2)
Short name:
GGT 5
Alternative name(s):
Gamma-glutamyl leukotrienase
Short name:
GGL
Gamma-glutamyltransferase-like activity 1
Gamma-glutamyltranspeptidase 5
Glutathione hydrolase 5 (EC:3.4.19.13)
Leukotriene-C4 hydrolase (EC:3.4.19.14)
Cleaved into the following 2 chains:
Gene namesi
Name:Ggt5
Synonyms:Ggtla1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:1346063. Ggt5.

Subcellular locationi

Membrane By similarity; Single-pass type II membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence Analysis
Transmembranei9 – 2921Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini30 – 573544ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Gamma-glutamyltransferase 5 heavy chainBy similarityPRO_0000011074Add
BLAST
Chaini389 – 573185Gamma-glutamyltransferase 5 light chainBy similarityPRO_0000011075Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
Glycosylationi185 – 1851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence Analysis
Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi347 – 3471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Cleaved by autocatalysis into a large and a small subunit.By similarity

Keywords - PTMi

Glycoprotein, Zymogen

Proteomic databases

MaxQBiQ9Z2A9.
PaxDbiQ9Z2A9.
PRIDEiQ9Z2A9.

PTM databases

PhosphoSiteiQ9Z2A9.

Expressioni

Tissue specificityi

Very low level of expression. Detected in spleen lymphocytes, medullary and paracortical thymic lymphocytes, lung interstitial cells, bronchial epithelium, proximal tubules in kidney, crypt cells in small intestine, neurons in brain stem and cerebral cortex and in Purkinje cells.

Gene expression databases

CleanExiMM_GGT5.
ExpressionAtlasiQ9Z2A9. baseline and differential.
GenevestigatoriQ9Z2A9.

Interactioni

Subunit structurei

Heterodimer composed of the light and heavy chains. The active site is located in the light chain (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000072074.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2A9.
SMRiQ9Z2A9. Positions 41-377, 389-573.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni454 – 4552Glutamate bindingBy similarity

Sequence similaritiesi

Belongs to the gamma-glutamyltransferase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0405.
GeneTreeiENSGT00550000074591.
HOGENOMiHOG000175620.
HOVERGENiHBG005835.
InParanoidiQ9Z2A9.
KOiK18592.
OMAiICTSVMN.
OrthoDBiEOG7V7665.
TreeFamiTF313608.

Family and domain databases

InterProiIPR000101. GGT_peptidase.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
PANTHERiPTHR11686. PTHR11686. 1 hit.
PfamiPF01019. G_glu_transpept. 1 hit.
[Graphical view]
PRINTSiPR01210. GGTRANSPTASE.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00462. G_GLU_TRANSPEPTIDASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9Z2A9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAWGHRATVC LVLLGVGLGL VIVVLAAVLS PRQASCGPGA FTRAAVAADS
60 70 80 90 100
KICSDIGRAI LQQRGSPVDA AIAALVCTGV VNPQSMGLGG GVVFTIYNAS
110 120 130 140 150
TGKVEIINAR ETVPASYDQG LLNQCKNVLP LGTGAQWIGV PGELRGYAEA
160 170 180 190 200
HRRHGRLPWA QLFQPTIALL REGFRVPFIL SQFLNNSILR PHLSASTLRQ
210 220 230 240 250
LFFNGTETLR SQDPFPWPAL ANTLETVAKE GAEVLYTGRL GRMLVEDIAK
260 270 280 290 300
QGSLLTVQDL AAFQPEVVEP LEMPLGNYTL YSPPPPAGGA ILSFILNVLK
310 320 330 340 350
GFNFSAETVA RPGGEVNMYH HLVETLKFAV GQRWRLWDPS SHPGIQNISR
360 370 380 390 400
DLLREDLAQR IRQQIDGRGD HHQLSHYNLT GVRGNRMGTS HVSVLGEDGS
410 420 430 440 450
AVAATSTINT PFGAMVYSPR TGILLNNELL DLCWRHMPTS PITPPPVPGE
460 470 480 490 500
RPPSSMVPSI LVNKGQGSKL VIGGAGGELI ISAVAQTIMN KLWLGFDLTE
510 520 530 540 550
AIASPILHVN SKGHVEYEPK FNQEVQKGLQ DRGQIQSQSQ RPVFLNAVQA
560 570
VFQEGPCVYA ASDLRKAGKA SGY
Length:573
Mass (Da):61,674
Last modified:September 18, 2013 - v2
Checksum:iCB00F377DA1BE83E
GO
Isoform 2 (identifier: Q9Z2A9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     447-456: VPGERPPSSM → DYHEQAVAWL
     457-573: Missing.

Note: Very low expression.

Show »
Length:456
Mass (Da):49,360
Checksum:iBEEEBF6F12478985
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti479 – 4791L → P in AAC71001. (PubMed:9774450)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei447 – 45610VPGERPPSSM → DYHEQAVAWL in isoform 2. 1 PublicationVSP_008147
Alternative sequencei457 – 573117Missing in isoform 2. 1 PublicationVSP_008148Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077765 mRNA. Translation: AAC71001.1.
AK052183 mRNA. Translation: BAC34873.1.
AK170326 mRNA. Translation: BAE41720.1.
AC087540 Genomic DNA. No translation available.
CH466553 Genomic DNA. Translation: EDL31901.1.
BC113775 mRNA. Translation: AAI13776.1.
CCDSiCCDS23928.1. [Q9Z2A9-1]
RefSeqiNP_035950.2. NM_011820.4. [Q9Z2A9-1]
UniGeneiMm.257927.

Genome annotation databases

EnsembliENSMUST00000072217; ENSMUSP00000072074; ENSMUSG00000006344. [Q9Z2A9-1]
GeneIDi23887.
KEGGimmu:23887.
UCSCiuc007fqq.1. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077765 mRNA. Translation: AAC71001.1.
AK052183 mRNA. Translation: BAC34873.1.
AK170326 mRNA. Translation: BAE41720.1.
AC087540 Genomic DNA. No translation available.
CH466553 Genomic DNA. Translation: EDL31901.1.
BC113775 mRNA. Translation: AAI13776.1.
CCDSiCCDS23928.1. [Q9Z2A9-1]
RefSeqiNP_035950.2. NM_011820.4. [Q9Z2A9-1]
UniGeneiMm.257927.

3D structure databases

ProteinModelPortaliQ9Z2A9.
SMRiQ9Z2A9. Positions 41-377, 389-573.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000072074.

Protein family/group databases

MEROPSiT03.002.

PTM databases

PhosphoSiteiQ9Z2A9.

Proteomic databases

MaxQBiQ9Z2A9.
PaxDbiQ9Z2A9.
PRIDEiQ9Z2A9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000072217; ENSMUSP00000072074; ENSMUSG00000006344. [Q9Z2A9-1]
GeneIDi23887.
KEGGimmu:23887.
UCSCiuc007fqq.1. mouse.

Organism-specific databases

CTDi2687.
MGIiMGI:1346063. Ggt5.

Phylogenomic databases

eggNOGiCOG0405.
GeneTreeiENSGT00550000074591.
HOGENOMiHOG000175620.
HOVERGENiHBG005835.
InParanoidiQ9Z2A9.
KOiK18592.
OMAiICTSVMN.
OrthoDBiEOG7V7665.
TreeFamiTF313608.

Enzyme and pathway databases

UniPathwayiUPA00204.
UPA00880.
ReactomeiREACT_215561. Synthesis of Leukotrienes (LT) and Eoxins (EX).
SABIO-RKQ9Z2A9.

Miscellaneous databases

NextBioi303635.
PROiQ9Z2A9.
SOURCEiSearch...

Gene expression databases

CleanExiMM_GGT5.
ExpressionAtlasiQ9Z2A9. baseline and differential.
GenevestigatoriQ9Z2A9.

Family and domain databases

InterProiIPR000101. GGT_peptidase.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
PANTHERiPTHR11686. PTHR11686. 1 hit.
PfamiPF01019. G_glu_transpept. 1 hit.
[Graphical view]
PRINTSiPR01210. GGTRANSPTASE.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00462. G_GLU_TRANSPEPTIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gamma-glutamyl leukotrienase, a gamma-glutamyl transpeptidase gene family member, is expressed primarily in spleen."
    Carter B.Z., Shi Z.-Z., Barrios R., Lieberman M.W.
    J. Biol. Chem. 273:28277-28285(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6 X 129.
    Tissue: Spleen.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Heart.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiGGT5_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2A9
Secondary accession number(s): Q8C7B4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: September 18, 2013
Last modified: January 7, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.