Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9Z2A9 (GGT5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gamma-glutamyltransferase 5

Short name=GGT 5
EC=2.3.2.2
Alternative name(s):
Gamma-glutamyl leukotrienase
Short name=GGL
Gamma-glutamyltransferase-like activity 1
Gamma-glutamyltranspeptidase 5
Glutathione hydrolase 5
EC=3.4.19.13
Leukotriene-C4 hydrolase
EC=3.4.19.14
Gene names
Name:Ggt5
Synonyms:Ggtla1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length573 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cleaves the gamma-glutamyl peptide bond of glutathione conjugates, but maybe not glutathione itself. Converts leukotriene C4 (LTC4) to leukotriene D4 (LTD4).

Catalytic activity

A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid.

Glutathione + H2O = L-cysteinylglycine + L-glutamate.

Leukotriene C4 + H2O = leukotriene D4 + L-glutamate.

Pathway

Sulfur metabolism; glutathione metabolism.

Lipid metabolism; leukotriene D4 biosynthesis.

Subunit structure

Heterodimer composed of the light and heavy chains. The active site is located in the light chain By similarity.

Subcellular location

Membrane; Single-pass type II membrane protein By similarity.

Tissue specificity

Very low level of expression. Detected in spleen lymphocytes, medullary and paracortical thymic lymphocytes, lung interstitial cells, bronchial epithelium, proximal tubules in kidney, crypt cells in small intestine, neurons in brain stem and cerebral cortex and in Purkinje cells.

Post-translational modification

Cleaved by autocatalysis into a large and a small subunit By similarity.

Sequence similarities

Belongs to the gamma-glutamyltransferase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Z2A9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Z2A9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     447-456: VPGERPPSSM → DYHEQAVAWL
     457-573: Missing.
Note: Very low expression.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 388388Gamma-glutamyltransferase 5 heavy chain By similarity
PRO_0000011074
Chain389 – 573185Gamma-glutamyltransferase 5 light chain By similarity
PRO_0000011075

Regions

Topological domain1 – 88Cytoplasmic Potential
Transmembrane9 – 2921Helical; Signal-anchor for type II membrane protein; Potential
Topological domain30 – 573544Extracellular Potential
Region454 – 4552Glutamate binding By similarity

Sites

Active site3891Nucleophile By similarity
Binding site1101Glutamate By similarity
Binding site4071Glutamate By similarity
Binding site4281Glutamate By similarity

Amino acid modifications

Glycosylation981N-linked (GlcNAc...) Potential
Glycosylation1851N-linked (GlcNAc...) Potential
Glycosylation2041N-linked (GlcNAc...) Potential
Glycosylation2771N-linked (GlcNAc...) Potential
Glycosylation3031N-linked (GlcNAc...) Potential
Glycosylation3471N-linked (GlcNAc...) Potential
Glycosylation3781N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence447 – 45610VPGERPPSSM → DYHEQAVAWL in isoform 2.
VSP_008147
Alternative sequence457 – 573117Missing in isoform 2.
VSP_008148

Experimental info

Sequence conflict4791L → P in AAC71001. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 18, 2013. Version 2.
Checksum: CB00F377DA1BE83E

FASTA57361,674
        10         20         30         40         50         60 
MAWGHRATVC LVLLGVGLGL VIVVLAAVLS PRQASCGPGA FTRAAVAADS KICSDIGRAI 

        70         80         90        100        110        120 
LQQRGSPVDA AIAALVCTGV VNPQSMGLGG GVVFTIYNAS TGKVEIINAR ETVPASYDQG 

       130        140        150        160        170        180 
LLNQCKNVLP LGTGAQWIGV PGELRGYAEA HRRHGRLPWA QLFQPTIALL REGFRVPFIL 

       190        200        210        220        230        240 
SQFLNNSILR PHLSASTLRQ LFFNGTETLR SQDPFPWPAL ANTLETVAKE GAEVLYTGRL 

       250        260        270        280        290        300 
GRMLVEDIAK QGSLLTVQDL AAFQPEVVEP LEMPLGNYTL YSPPPPAGGA ILSFILNVLK 

       310        320        330        340        350        360 
GFNFSAETVA RPGGEVNMYH HLVETLKFAV GQRWRLWDPS SHPGIQNISR DLLREDLAQR 

       370        380        390        400        410        420 
IRQQIDGRGD HHQLSHYNLT GVRGNRMGTS HVSVLGEDGS AVAATSTINT PFGAMVYSPR 

       430        440        450        460        470        480 
TGILLNNELL DLCWRHMPTS PITPPPVPGE RPPSSMVPSI LVNKGQGSKL VIGGAGGELI 

       490        500        510        520        530        540 
ISAVAQTIMN KLWLGFDLTE AIASPILHVN SKGHVEYEPK FNQEVQKGLQ DRGQIQSQSQ 

       550        560        570 
RPVFLNAVQA VFQEGPCVYA ASDLRKAGKA SGY 

« Hide

Isoform 2 [UniParc].

Checksum: BEEEBF6F12478985
Show »

FASTA45649,360

References

« Hide 'large scale' references
[1]"Gamma-glutamyl leukotrienase, a gamma-glutamyl transpeptidase gene family member, is expressed primarily in spleen."
Carter B.Z., Shi Z.-Z., Barrios R., Lieberman M.W.
J. Biol. Chem. 273:28277-28285(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6 X 129.
Tissue: Spleen.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Heart.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF077765 mRNA. Translation: AAC71001.1.
AK052183 mRNA. Translation: BAC34873.1.
AK170326 mRNA. Translation: BAE41720.1.
AC087540 Genomic DNA. No translation available.
CH466553 Genomic DNA. Translation: EDL31901.1.
BC113775 mRNA. Translation: AAI13776.1.
RefSeqNP_035950.2. NM_011820.4.
UniGeneMm.257927.

3D structure databases

ProteinModelPortalQ9Z2A9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000072074.

Protein family/group databases

MEROPST03.002.

PTM databases

PhosphoSiteQ9Z2A9.

Proteomic databases

PaxDbQ9Z2A9.
PRIDEQ9Z2A9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000072217; ENSMUSP00000072074; ENSMUSG00000006344. [Q9Z2A9-1]
GeneID23887.
KEGGmmu:23887.
UCSCuc007fqq.1. mouse.

Organism-specific databases

CTD2687.
MGIMGI:1346063. Ggt5.

Phylogenomic databases

eggNOGCOG0405.
GeneTreeENSGT00550000074591.
HOGENOMHOG000175620.
HOVERGENHBG005835.
InParanoidQ9Z2A9.
KOK00681.
OMAATHQPRI.
OrthoDBEOG7V7665.
TreeFamTF313608.

Enzyme and pathway databases

UniPathwayUPA00204.
UPA00880.

Gene expression databases

CleanExMM_GGT5.
GenevestigatorQ9Z2A9.

Family and domain databases

InterProIPR000101. GGT_peptidase.
[Graphical view]
PANTHERPTHR11686. PTHR11686. 1 hit.
PfamPF01019. G_glu_transpept. 1 hit.
[Graphical view]
PRINTSPR01210. GGTRANSPTASE.
PROSITEPS00462. G_GLU_TRANSPEPTIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio303635.
PROQ9Z2A9.
SOURCESearch...

Entry information

Entry nameGGT5_MOUSE
AccessionPrimary (citable) accession number: Q9Z2A9
Secondary accession number(s): Q8C7B4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: September 18, 2013
Last modified: April 16, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot