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Q9Z2A7 (DGAT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diacylglycerol O-acyltransferase 1
EC=2.3.1.20
Alternative name(s):
Diglyceride acyltransferase
Gene names
Name:Dgat1
Synonyms:Dgat
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length498 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. Ref.6

Catalytic activity

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol. Ref.6

Pathway

Lipid metabolism; glycerolipid metabolism.

Subunit structure

Homodimer or homotetramer. Ref.6

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.6.

Disruption phenotype

Mice live well but display reduced postabsorptive chylomicronemia and accumulate neutral-lipid droplets in the cytoplasm of enterocytes when chronically fed a high fat diet. Ref.4

Sequence similarities

Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 498498Diacylglycerol O-acyltransferase 1
PRO_0000207655

Regions

Topological domain1 – 9696Cytoplasmic Potential
Transmembrane97 – 11822Helical; Potential
Topological domain119 – 230112Lumenal Potential
Transmembrane231 – 24010Helical; Potential
Topological domain2411Cytoplasmic Potential
Transmembrane242 – 2509Helical; Potential
Topological domain251 – 498248Lumenal Potential
Region1 – 100100Involved in homomerization

Sites

Active site4261 Probable

Amino acid modifications

Modified residue201Phosphoserine Ref.5

Experimental info

Mutagenesis4261H → A: Impairs the ability to synthesize triacylglycerols, as well as retinyl and wax esters, in an in vitro acyltransferase assay. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q9Z2A7 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: E7B0DD6DDCF1EC2B

FASTA49856,790
        10         20         30         40         50         60 
MGDRGGAGSS RRRRTGSRVS VQGGSGPKVE EDEVRDAAVS PDLGAGGDAP APAPAPAHTR 

        70         80         90        100        110        120 
DKDGRTSVGD GYWDLRCHRL QDSLFSSDSG FSNYRGILNW CVVMLILSNA RLFLENLIKY 

       130        140        150        160        170        180 
GILVDPIQVV SLFLKDPYSW PAPCVIIASN IFVVAAFQIE KRLAVGALTE QMGLLLHVVN 

       190        200        210        220        230        240 
LATIICFPAA VALLVESITP VGSVFALASY SIMFLKLYSY RDVNLWCRQR RVKAKAVSTG 

       250        260        270        280        290        300 
KKVSGAAAQQ AVSYPDNLTY RDLYYFIFAP TLCYELNFPR SPRIRKRFLL RRVLEMLFFT 

       310        320        330        340        350        360 
QLQVGLIQQW MVPTIQNSMK PFKDMDYSRI IERLLKLAVP NHLIWLIFFY WFFHSCLNAV 

       370        380        390        400        410        420 
AELLQFGDRE FYRDWWNAES VTYFWQNWNI PVHKWCIRHF YKPMLRHGSS KWVARTGVFL 

       430        440        450        460        470        480 
TSAFFHEYLV SVPLRMFRLW AFTAMMAQVP LAWIVGRFFQ GNYGNAAVWV TLIIGQPVAV 

       490 
LMYVHDYYVL NYDAPVGV

« Hide

References

« Hide 'large scale' references
[1]"Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis."
Cases S., Smith S.J., Zheng Y.-W., Myers H.M., Lear S.R., Sande E., Novak S., Collins C., Welch C.B., Lusis A.J., Erickson S.K., Farese R.V. Jr.
Proc. Natl. Acad. Sci. U.S.A. 95:13018-13023(1998) [PubMed: 9789033] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Tongue.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"DGAT1 is not essential for intestinal triacylglycerol absorption or chylomicron synthesis."
Buhman K.K., Smith S.J., Stone S.J., Repa J.J., Wong J.S., Knapp F.F.R. Jr., Burri B.J., Hamilton R.L., Abumrad N.A., Farese R.V. Jr.
J. Biol. Chem. 277:25474-25479(2002) [PubMed: 11959864] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20, MASS SPECTROMETRY.
Tissue: Liver.
[6]"Acyl CoA:Diacylglycerol acyltransferase-1 (DGAT1): topological orientation, identification of a putative active site histidine and the role of the N-terminus in dimer/tetramer formation."
McFie P.J., Stone S.L., Banman S.L., Stone S.J.
J. Biol. Chem. 285:37377-37387(2010) [PubMed: 20876538] [Abstract]
Cited for: MUTAGENESIS OF HIS-426, SUBUNIT, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, MEMBRANE TOPOLOGY, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF078752 mRNA. Translation: AAC72917.1.
AK008995 mRNA. No translation available.
BC003717 mRNA. Translation: AAH03717.1.
IPIIPI00130963.
RefSeqNP_034176.1. NM_010046.2.
UniGeneMm.22633.

3D structure databases

ProteinModelPortalQ9Z2A7.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9Z2A7.

PTM databases

PhosphoSiteQ9Z2A7.

Proteomic databases

PRIDEQ9Z2A7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023214; ENSMUSP00000023214; ENSMUSG00000022555.
GeneID13350.
KEGGmmu:13350.
NMPDRfig|10090.3.peg.30145.

Organism-specific databases

CTD8694.
MGIMGI:1333825. Dgat1.

Phylogenomic databases

GeneTreeENSGT00530000063122.
HOGENOMHBG326701.
HOVERGENHBG051341.
InParanoidQ9Z2A7.
OMANIDTFWR.
OrthoDBEOG4VT5X5.
PhylomeDBQ9Z2A7.

Enzyme and pathway databases

BRENDA2.3.1.20. 3474.

Gene expression databases

ArrayExpressQ9Z2A7.
BgeeQ9Z2A7.
CleanExMM_DGAT1.
GenevestigatorQ9Z2A7.
GermOnlineENSMUSG00000022555. Mus musculus.

Family and domain databases

InterProIPR004299. MBOAT_fam.
IPR014371. Oat_ACAT_DAG_ARE.
[Graphical view]
KOK11155.
PANTHERPTHR10408. PTHR10408. 1 hit.
PfamPF03062. MBOAT. 1 hit.
[Graphical view]
PIRSFPIRSF000439. Oat_ACAT_DAG_ARE. 1 hit.
ProtoNetSearch...

Other

NextBio283676.
SOURCESearch...

Entry information

Entry nameDGAT1_MOUSE
AccessionPrimary (citable) accession number: Q9Z2A7
Secondary accession number(s): Q9D7Q5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 1, 1999
Last modified: November 16, 2011
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents