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Protein

Mitogen-activated protein kinase 15

Gene

Mapk15

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Constitutively active kinase which may function as a negative regulator of cell growth. In vitro, phosphorylates FOS.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43ATPPROSITE-ProRule annotation1
Active sitei138Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 28ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • dopamine uptake Source: UniProtKB
  • endoplasmic reticulum organization Source: UniProtKB
  • negative regulation of cell migration Source: UniProtKB
  • negative regulation of DNA replication Source: RGD
  • negative regulation of transcription by RNA polymerase II Source: RGD
  • positive regulation of protein catabolic process Source: RGD
  • positive regulation of protein phosphorylation Source: RGD
  • positive regulation of protein ubiquitination Source: RGD
  • protein autophosphorylation Source: RGD
  • protein phosphorylation Source: RGD
  • regulation of autophagy Source: UniProtKB
  • regulation of cilium assembly Source: UniProtKB
  • regulation of COPII vesicle coating Source: UniProtKB
  • response to estradiol Source: RGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 15 (EC:2.7.11.24)
Short name:
MAP kinase 15
Short name:
MAPK 15
Alternative name(s):
Extracellular signal-regulated kinase 7
Short name:
ERK-7
Gene namesi
Name:Mapk15
Synonyms:Erk7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi628675 Mapk15

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi43K → R: Loss of autophosphorylation and activity. 1 Publication1
Mutagenesisi176T → A: Loss of autophosphorylation and activity. Loss of autophosphorylation and activity; when associated with F-178. 1 Publication1
Mutagenesisi178Y → F: Loss of autophosphorylation and activity. Loss of autophosphorylation and activity; when associated with A-176. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002326391 – 547Mitogen-activated protein kinase 15Add BLAST547

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei176Phosphothreonine1 Publication1
Modified residuei178Phosphotyrosine1 Publication1
Modified residuei449Omega-N-methylarginineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-176 and Tyr-178, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues.1 Publication
Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.1 Publication

Keywords - PTMi

Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9Z2A6
PRIDEiQ9Z2A6

PTM databases

iPTMnetiQ9Z2A6

Expressioni

Tissue specificityi

Ubiquitously expressed at a weak level. Highest expression is found in testis and to a lower extent in lung.2 Publications

Gene expression databases

BgeeiENSRNOG00000009336
ExpressionAtlasiQ9Z2A6 baseline and differential
GenevisibleiQ9Z2A6 RN

Interactioni

Subunit structurei

Interacts with TGFB1I1.

Protein-protein interaction databases

IntActiQ9Z2A6, 1 interactor
MINTiQ9Z2A6
STRINGi10116.ENSRNOP00000012460

Structurei

3D structure databases

ProteinModelPortaliQ9Z2A6
SMRiQ9Z2A6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 305Protein kinasePROSITE-ProRule annotationAdd BLAST292

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 20Ubiquitin-conjugatingAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi176 – 178TXY3

Domaini

C-terminal domain, rather than the kinase activity, is required for the full function of the enzyme. This region may be a protein interaction domain that regulates kinase localization, activation and transcriptional activity. When C-terminally truncated, the enzyme shows a reduction in the Thr-Glu-Tyr (TEY) phosphorylation level, in the in vitro kinase activity, in its nuclear localization and in its inhibitory effect on cell growth.
The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
GeneTreeiENSGT00910000144035
HOGENOMiHOG000233024
HOVERGENiHBG014652
InParanoidiQ9Z2A6
KOiK19603
PhylomeDBiQ9Z2A6
TreeFamiTF105101

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Z2A6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCAAEVDRHV SQRYLIKRRL GKGAYGIVWK AMDRRTGEVV AIKKIFDAFR
60 70 80 90 100
DQTDAQRTFR EIMLLREFGG HPNIIRLLDV IPAKNDRDIY LVFESMDTDL
110 120 130 140 150
NAVIQKGRLL EDIHKRCIFY QLLRATKFIH SGRVIHRDQK PANVLLDAAC
160 170 180 190 200
RVKLCDFGLA RSLSDFPEGP GGQALTEYVA TRWYRAPEVL LSSRWYTPGV
210 220 230 240 250
DMWSLGCILG EMLRGQPLFP GTSTFHQLEL ILETIPLPSM EELQGLGSDY
260 270 280 290 300
SALILQNLGS RPRQTLDALL PPDTPPEALD LLKRLLAFAP DKRLSAEQAL
310 320 330 340 350
QHPYVQRFHC PDREWTRGSD VRLPVHEGDQ LSAPEYRNRL YQMILERRRN
360 370 380 390 400
SRSPREEDLG VVASRAELRA SQRQSLKPGV LPQVLAETPA RKRGPKPQNG
410 420 430 440 450
HGHDPEHVEV RRQSSDPLYQ LPPPGSGERP PGATGEPPSA PSGVKTHVRA
460 470 480 490 500
VAPSLTSQAA AQAANQPLIR SDPARGGGPR AVGARRVPSR LPREAPEPRP
510 520 530 540
GRRMFGISVS QGAQGAARAA LGGYSQAYGT VCRSALGRLP LLPGPRA
Length:547
Mass (Da):60,724
Last modified:March 1, 2002 - v2
Checksum:iD8B7D9955B06862F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF078798 mRNA Translation: AAD12719.2
RefSeqiNP_775453.1, NM_173331.2
XP_006241841.1, XM_006241779.3
UniGeneiRn.42898

Genome annotation databases

EnsembliENSRNOT00000012461; ENSRNOP00000012460; ENSRNOG00000009336
GeneIDi286997
KEGGirno:286997

Similar proteinsi

Entry informationi

Entry nameiMK15_RAT
AccessioniPrimary (citable) accession number: Q9Z2A6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: March 1, 2002
Last modified: May 23, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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