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Q9Z2A6

- MK15_RAT

UniProt

Q9Z2A6 - MK15_RAT

Protein

Mitogen-activated protein kinase 15

Gene

Mapk15

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Constitutively active kinase which may function as a negative regulator of cell growth. In vitro, phosphorylates FOS.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei43 – 431ATPPROSITE-ProRule annotation
    Active sitei138 – 1381Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi20 – 289ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. negative regulation of DNA replication Source: RGD
    2. negative regulation of transcription from RNA polymerase II promoter Source: RGD
    3. positive regulation of protein catabolic process Source: RGD
    4. positive regulation of protein phosphorylation Source: RGD
    5. positive regulation of protein ubiquitination Source: RGD
    6. protein autophosphorylation Source: UniProtKB
    7. protein phosphorylation Source: RGD
    8. response to estradiol Source: RGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 15 (EC:2.7.11.24)
    Short name:
    MAP kinase 15
    Short name:
    MAPK 15
    Alternative name(s):
    Extracellular signal-regulated kinase 7
    Short name:
    ERK-7
    Gene namesi
    Name:Mapk15
    Synonyms:Erk7
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi628675. Mapk15.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi43 – 431K → R: Loss of autophosphorylation and activity. 1 Publication
    Mutagenesisi176 – 1761T → A: Loss of autophosphorylation and activity. Loss of autophosphorylation and activity; when associated with F-178. 1 Publication
    Mutagenesisi178 – 1781Y → F: Loss of autophosphorylation and activity. Loss of autophosphorylation and activity; when associated with A-176. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 547547Mitogen-activated protein kinase 15PRO_0000232639Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei176 – 1761Phosphothreonine1 Publication
    Modified residuei178 – 1781Phosphotyrosine1 Publication
    Modified residuei415 – 4151PhosphoserineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-176 and Tyr-178, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues.1 Publication
    Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.1 Publication

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ9Z2A6.
    PRIDEiQ9Z2A6.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed at a weak level. Highest expression is found in testis and to a lower extent in lung.2 Publications

    Gene expression databases

    GenevestigatoriQ9Z2A6.

    Interactioni

    Subunit structurei

    Interacts with TGFB1I1.

    Protein-protein interaction databases

    IntActiQ9Z2A6. 1 interaction.
    STRINGi10116.ENSRNOP00000012460.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z2A6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini14 – 305292Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2020Ubiquitin-conjugatingAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi176 – 1783TXY

    Domaini

    C-terminal domain, rather than the kinase activity, is required for the full function of the enzyme. This region may be a protein interaction domain that regulates kinase localization, activation and transcriptional activity. When C-terminally truncated, the enzyme shows a reduction in the Thr-Glu-Tyr (TEY) phosphorylation level, in the in vitro kinase activity, in its nuclear localization and in its inhibitory effect on cell growth.
    The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation.
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00730000110881.
    HOGENOMiHOG000233024.
    HOVERGENiHBG014652.
    InParanoidiQ9Z2A6.
    KOiK08293.
    OMAiCTVKLCD.
    OrthoDBiEOG7VDXPC.
    PhylomeDBiQ9Z2A6.
    TreeFamiTF105101.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z2A6-1 [UniParc]FASTAAdd to Basket

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    MCAAEVDRHV SQRYLIKRRL GKGAYGIVWK AMDRRTGEVV AIKKIFDAFR    50
    DQTDAQRTFR EIMLLREFGG HPNIIRLLDV IPAKNDRDIY LVFESMDTDL 100
    NAVIQKGRLL EDIHKRCIFY QLLRATKFIH SGRVIHRDQK PANVLLDAAC 150
    RVKLCDFGLA RSLSDFPEGP GGQALTEYVA TRWYRAPEVL LSSRWYTPGV 200
    DMWSLGCILG EMLRGQPLFP GTSTFHQLEL ILETIPLPSM EELQGLGSDY 250
    SALILQNLGS RPRQTLDALL PPDTPPEALD LLKRLLAFAP DKRLSAEQAL 300
    QHPYVQRFHC PDREWTRGSD VRLPVHEGDQ LSAPEYRNRL YQMILERRRN 350
    SRSPREEDLG VVASRAELRA SQRQSLKPGV LPQVLAETPA RKRGPKPQNG 400
    HGHDPEHVEV RRQSSDPLYQ LPPPGSGERP PGATGEPPSA PSGVKTHVRA 450
    VAPSLTSQAA AQAANQPLIR SDPARGGGPR AVGARRVPSR LPREAPEPRP 500
    GRRMFGISVS QGAQGAARAA LGGYSQAYGT VCRSALGRLP LLPGPRA 547
    Length:547
    Mass (Da):60,724
    Last modified:March 1, 2002 - v2
    Checksum:iD8B7D9955B06862F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF078798 mRNA. Translation: AAD12719.2.
    RefSeqiNP_775453.1. NM_173331.2.
    XP_006241841.1. XM_006241779.1.
    UniGeneiRn.42898.

    Genome annotation databases

    EnsembliENSRNOT00000012461; ENSRNOP00000012460; ENSRNOG00000009336.
    GeneIDi286997.
    KEGGirno:286997.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF078798 mRNA. Translation: AAD12719.2 .
    RefSeqi NP_775453.1. NM_173331.2.
    XP_006241841.1. XM_006241779.1.
    UniGenei Rn.42898.

    3D structure databases

    ProteinModelPortali Q9Z2A6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9Z2A6. 1 interaction.
    STRINGi 10116.ENSRNOP00000012460.

    Proteomic databases

    PaxDbi Q9Z2A6.
    PRIDEi Q9Z2A6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000012461 ; ENSRNOP00000012460 ; ENSRNOG00000009336 .
    GeneIDi 286997.
    KEGGi rno:286997.

    Organism-specific databases

    CTDi 225689.
    RGDi 628675. Mapk15.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00730000110881.
    HOGENOMi HOG000233024.
    HOVERGENi HBG014652.
    InParanoidi Q9Z2A6.
    KOi K08293.
    OMAi CTVKLCD.
    OrthoDBi EOG7VDXPC.
    PhylomeDBi Q9Z2A6.
    TreeFami TF105101.

    Miscellaneous databases

    NextBioi 625286.
    PROi Q9Z2A6.

    Gene expression databases

    Genevestigatori Q9Z2A6.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Extracellular signal-regulated kinase 7 (ERK7), a novel ERK with a C-terminal domain that regulates its activity, its cellular localization, and cell growth."
      Abe M.K., Kuo W.-L., Hershenson M.B., Rosner M.R.
      Mol. Cell. Biol. 19:1301-1312(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DOMAIN, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, CHARACTERIZATION, PHOSPHORYLATION AT THR-176 AND TYR-178, MUTAGENESIS OF LYS-43; THR-176; TYR-178 AND 176-THR--TYR-178.
      Strain: Sprague-Dawley.
      Tissue: Testis.
    2. Lubec G., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 154-161, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    3. Cited for: DOMAIN, CHARACTERIZATION.
    4. Cited for: FUNCTION.
    5. "ERK7 expression and kinase activity is regulated by the ubiquitin-proteosome pathway."
      Kuo W.-L., Duke C.J., Abe M.K., Kaplan E.L., Gomes S., Rosner M.R.
      J. Biol. Chem. 279:23073-23081(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiMK15_RAT
    AccessioniPrimary (citable) accession number: Q9Z2A6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2006
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3