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Q9Z2A6

- MK15_RAT

UniProt

Q9Z2A6 - MK15_RAT

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Protein
Mitogen-activated protein kinase 15
Gene
Mapk15, Erk7
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Constitutively active kinase which may function as a negative regulator of cell growth. In vitro, phosphorylates FOS.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431ATP By similarity
Active sitei138 – 1381Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 289ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB
  3. protein binding Source: UniProtKB

GO - Biological processi

  1. negative regulation of DNA replication Source: RGD
  2. negative regulation of transcription from RNA polymerase II promoter Source: RGD
  3. positive regulation of protein catabolic process Source: RGD
  4. positive regulation of protein phosphorylation Source: RGD
  5. positive regulation of protein ubiquitination Source: RGD
  6. protein autophosphorylation Source: UniProtKB
  7. protein phosphorylation Source: RGD
  8. response to estradiol Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 15 (EC:2.7.11.24)
Short name:
MAP kinase 15
Short name:
MAPK 15
Alternative name(s):
Extracellular signal-regulated kinase 7
Short name:
ERK-7
Gene namesi
Name:Mapk15
Synonyms:Erk7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 7

Organism-specific databases

RGDi628675. Mapk15.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi43 – 431K → R: Loss of autophosphorylation and activity. 1 Publication
Mutagenesisi176 – 1761T → A: Loss of autophosphorylation and activity. Loss of autophosphorylation and activity; when associated with F-178. 1 Publication
Mutagenesisi178 – 1781Y → F: Loss of autophosphorylation and activity. Loss of autophosphorylation and activity; when associated with A-176. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 547547Mitogen-activated protein kinase 15
PRO_0000232639Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei176 – 1761Phosphothreonine1 Publication
Modified residuei178 – 1781Phosphotyrosine1 Publication
Modified residuei415 – 4151Phosphoserine By similarity

Post-translational modificationi

Dually phosphorylated on Thr-176 and Tyr-178, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues.1 Publication
Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9Z2A6.
PRIDEiQ9Z2A6.

Expressioni

Tissue specificityi

Ubiquitously expressed at a weak level. Highest expression is found in testis and to a lower extent in lung.2 Publications

Gene expression databases

GenevestigatoriQ9Z2A6.

Interactioni

Subunit structurei

Interacts with TGFB1I1.

Protein-protein interaction databases

IntActiQ9Z2A6. 1 interaction.
STRINGi10116.ENSRNOP00000012460.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2A6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 305292Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2020Ubiquitin-conjugating
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi176 – 1783TXY

Domaini

C-terminal domain, rather than the kinase activity, is required for the full function of the enzyme. This region may be a protein interaction domain that regulates kinase localization, activation and transcriptional activity. When C-terminally truncated, the enzyme shows a reduction in the Thr-Glu-Tyr (TEY) phosphorylation level, in the in vitro kinase activity, in its nuclear localization and in its inhibitory effect on cell growth.2 Publications
The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation.2 Publications
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.2 Publications

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00730000110881.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ9Z2A6.
KOiK08293.
OMAiCTVKLCD.
OrthoDBiEOG7VDXPC.
PhylomeDBiQ9Z2A6.
TreeFamiTF105101.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z2A6-1 [UniParc]FASTAAdd to Basket

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MCAAEVDRHV SQRYLIKRRL GKGAYGIVWK AMDRRTGEVV AIKKIFDAFR    50
DQTDAQRTFR EIMLLREFGG HPNIIRLLDV IPAKNDRDIY LVFESMDTDL 100
NAVIQKGRLL EDIHKRCIFY QLLRATKFIH SGRVIHRDQK PANVLLDAAC 150
RVKLCDFGLA RSLSDFPEGP GGQALTEYVA TRWYRAPEVL LSSRWYTPGV 200
DMWSLGCILG EMLRGQPLFP GTSTFHQLEL ILETIPLPSM EELQGLGSDY 250
SALILQNLGS RPRQTLDALL PPDTPPEALD LLKRLLAFAP DKRLSAEQAL 300
QHPYVQRFHC PDREWTRGSD VRLPVHEGDQ LSAPEYRNRL YQMILERRRN 350
SRSPREEDLG VVASRAELRA SQRQSLKPGV LPQVLAETPA RKRGPKPQNG 400
HGHDPEHVEV RRQSSDPLYQ LPPPGSGERP PGATGEPPSA PSGVKTHVRA 450
VAPSLTSQAA AQAANQPLIR SDPARGGGPR AVGARRVPSR LPREAPEPRP 500
GRRMFGISVS QGAQGAARAA LGGYSQAYGT VCRSALGRLP LLPGPRA 547
Length:547
Mass (Da):60,724
Last modified:March 1, 2002 - v2
Checksum:iD8B7D9955B06862F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF078798 mRNA. Translation: AAD12719.2.
RefSeqiNP_775453.1. NM_173331.2.
XP_006241841.1. XM_006241779.1.
UniGeneiRn.42898.

Genome annotation databases

EnsembliENSRNOT00000012461; ENSRNOP00000012460; ENSRNOG00000009336.
GeneIDi286997.
KEGGirno:286997.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF078798 mRNA. Translation: AAD12719.2 .
RefSeqi NP_775453.1. NM_173331.2.
XP_006241841.1. XM_006241779.1.
UniGenei Rn.42898.

3D structure databases

ProteinModelPortali Q9Z2A6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9Z2A6. 1 interaction.
STRINGi 10116.ENSRNOP00000012460.

Proteomic databases

PaxDbi Q9Z2A6.
PRIDEi Q9Z2A6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000012461 ; ENSRNOP00000012460 ; ENSRNOG00000009336 .
GeneIDi 286997.
KEGGi rno:286997.

Organism-specific databases

CTDi 225689.
RGDi 628675. Mapk15.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00730000110881.
HOGENOMi HOG000233024.
HOVERGENi HBG014652.
InParanoidi Q9Z2A6.
KOi K08293.
OMAi CTVKLCD.
OrthoDBi EOG7VDXPC.
PhylomeDBi Q9Z2A6.
TreeFami TF105101.

Miscellaneous databases

NextBioi 625286.
PROi Q9Z2A6.

Gene expression databases

Genevestigatori Q9Z2A6.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Extracellular signal-regulated kinase 7 (ERK7), a novel ERK with a C-terminal domain that regulates its activity, its cellular localization, and cell growth."
    Abe M.K., Kuo W.-L., Hershenson M.B., Rosner M.R.
    Mol. Cell. Biol. 19:1301-1312(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DOMAIN, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, CHARACTERIZATION, PHOSPHORYLATION AT THR-176 AND TYR-178, MUTAGENESIS OF LYS-43; THR-176; TYR-178 AND 176-THR--TYR-178.
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. Lubec G., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 154-161, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. Cited for: DOMAIN, CHARACTERIZATION.
  4. Cited for: FUNCTION.
  5. "ERK7 expression and kinase activity is regulated by the ubiquitin-proteosome pathway."
    Kuo W.-L., Duke C.J., Abe M.K., Kaplan E.L., Gomes S., Rosner M.R.
    J. Biol. Chem. 279:23073-23081(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiMK15_RAT
AccessioniPrimary (citable) accession number: Q9Z2A6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: March 1, 2002
Last modified: June 11, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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