Q9Z286 (ADCYA_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylate cyclase type 10
EC=4.6.1.1

Alternative name(s):
Germ cell soluble adenylyl cyclase
Short name=sAC
Testicular soluble adenylyl cyclase

Gene names

Name:	Adcy10
Synonyms:	Sac

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1608 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor By similarity.
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Enzyme regulation	Activated by manganese or magnesium ions. In the presence of magnesium ions, the enzyme is activated by bicarbonate while in the presence of manganese ions, the enzyme is inhibited by bicarbonate. In the absence of magnesium and bicarbonate, the enzyme is weakly activated by calcium By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm › cytoskeleton. Mitochondrion. Cytoplasm › perinuclear region. Cytoplasm › cytoskeleton › centrosome › centriole By similarity. Note: Distributed to subcellular compartments containing cAMP targets. Found as a plasma membrane-associated protein, protein concentrated in the perinuclear region and protein colocalized with actin, tubulin or centriole By similarity. Ref.1 Ref.3
Tissue specificity	Preferentially expressed in testis. Ref.1
Post-translational modification	Cleavage may occur to generate the active 48 kDa form.
Sequence similarities	Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 2 guanylate cyclase domains.
Biophysicochemical properties	Kinetic parameters: KM=1 mM for ATP-Mn2+ Ref.1 Ref.4

Ontologies

Keywords
Biological process	cAMP biosynthesis
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro positive regulation of apoptotic process Inferred from mutant phenotype PubMed 19336406. Source: RGD spermatogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	apical part of cell Inferred from direct assay PubMed 17959750. Source: RGD axon Inferred from direct assay PubMed 16964251. Source: RGD basal part of cell Inferred from direct assay PubMed 17959750. Source: RGD centriole Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from direct assay Ref.1. Source: RGD dendrite Inferred from direct assay PubMed 16964251. Source: RGD growth cone Inferred from direct assay PubMed 16964251. Source: RGD mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell neuronal cell body Inferred from direct assay PubMed 16964251. Source: RGD perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Inferred from direct assay PubMed 7225326Ref.1. Source: RGD magnesium ion binding Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from direct assay PubMed 7225326. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced.

Isoform 1 (identifier: Q9Z286-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1608	1608	Adenylate cyclase type 10		PRO_0000317104
Regions
	Domain	42 – 179	138	Guanylate cyclase 1
	Domain	293 – 418	126	Guanylate cyclase 2
Sites
	Metal binding	47	1	Magnesium 1 By similarity
	Metal binding	47	1	Magnesium 2 By similarity
	Metal binding	48	1	Magnesium 2; via carbonyl oxygen By similarity
	Metal binding	99	1	Magnesium 1 By similarity
	Metal binding	99	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: BEF6D3F60A5D2556	FASTA	1,608	185,854
	10 20 30 40 50 60 MSARRQELQD RAIVKIAAHL PDLIVYGDFS PERPSVKCFD GVLMFVDISG FTAMTEKFST 70 80 90 100 110 120 AMYMDRGAEQ LVEILNYYIS AIVEKVLIFG GDILKFAGDA LLALWKVERK QLKNIITVVI 130 140 150 160 170 180 KCSLEIHGLF EAKEVEEGLD IRVKIGLAAG HITMLVFGDE TRNYFLVIGQ AVDDVRLAQN 190 200 210 220 230 240 MAQMNDVILS PNCWQLCDRS MIEIERIPDQ RAVKVSFLKP PPTFNFDEFF AKCMAFMDYY 250 260 270 280 290 300 PSGDHKNFLR LACMLESDPE LELSLQKYVM EIILKQIDDK QLRGYLSELR PVTIVFVNLM 310 320 330 340 350 360 FKEQDKAEVI GSAIQAACVH ITSVLKVFRG QINKVFMFDK GCSFLCVFGF PGEKAPDEIT 370 380 390 400 410 420 HALESAVDIF DFCSQVHKIR TVSIGVASGI VFCGIVGHTV RHEYTVIGQK VNIAARMMMY 430 440 450 460 470 480 YPGIVTCDSV TYDGSNLPAY FFKELPKKVM KGVADPGPVY QCLGLNEKVM FGMAYLICNR 490 500 510 520 530 540 YEGYPLLGRV REIDYFMSTM KDFLMTNCSR VLMYEGLPGY GKSQVLMEIE YLASQHENHR 550 560 570 580 590 600 AVAIALTKIS FHQNFYTIQI LMANVLGLDT CKHYKERQTN LQNRVKTLLD DKYHCLLNDI 610 620 630 640 650 660 FHVQFPVSRE MSRMSKIRKQ KQLEALFMKI LEQTVREERI IFIIDEAQFV DVASWAFIEK 670 680 690 700 710 720 LIRSMPIFIV MSLCPFPETP CAAANAIMKN RNTTYITLGT MQPQEIRDKV CVDLSVSSIP 730 740 750 760 770 780 RELDSYLVEG SCGIPYYCEE LLKNLDHHRI LIFQQAEAEE KTNVTWNNLF KYSVKPTEDM 790 800 810 820 830 840 YLYTSIAAGQ KEACYLTSGV RLKNLSPPAS LKEISLVQLD SMSLSHQMLV RCAAIIGLTF 850 860 870 880 890 900 TTELLFEILP CWNMKMMIKA LATLVESNVF DCFRSSKDLQ LALKQNVTTF EVHYRSLSLK 910 920 930 940 950 960 SKEGLAYSEE EQLREMEGEV IECRILRFCR PIMQKTAYEL WLKDQKKVLH LKCARFLEES 970 980 990 1000 1010 1020 AHRCNHCRNR DFIPYHHFIA DIRLNTLDMD TVKKMVKSHG FKTEDEVIFS KSEIPRKFKF 1030 1040 1050 1060 1070 1080 PENISITETR EKILHFFDNV IIKMRTSQDD VIPLESCHCE ELLQIVILPL AQHFVALEEN 1090 1100 1110 1120 1130 1140 NKALYYFLEL ASAYLILGDN YNAYMYLGEG ERLLKSLTNE DSWSQTFEYA TFYSLKGEIC 1150 1160 1170 1180 1190 1200 FNMGQMVLAK KMLRKALKLL NRMFPCNLLS LTFQMHIEKN RLSHFMNQHT QEGSLPGKKL 1210 1220 1230 1240 1250 1260 AQLFLQSSCF SLLWKIYSLN FFFHYKYYGR LAAIMQMNTS LETQNNFQII KAFLDFSLYR 1270 1280 1290 1300 1310 1320 HLAGYEGVWF KYEILVMEQL LNLPLKGEAF EIMAYAADAL GHIKFLTGHL DLAIELGSRA 1330 1340 1350 1360 1370 1380 HKMWSLLRNP NKYHMVLCRL SKPLFLKSRY KHLVQVLGWL WDLSVTEEHI FSKAFFYFVC 1390 1400 1410 1420 1430 1440 LDIMLYSGFI YRTFEECLEF IHHNEDNRIL KFQSGLLLGL YSCIAVWYAR LQEWDNFYKF 1450 1460 1470 1480 1490 1500 SNRAKTLVTR RTPTVLYYEG ISRYMEGQVL HLQKQIEEQA ENAQDSGVEL LKALETLVAQ 1510 1520 1530 1540 1550 1560 NTTGPVFYPR LYHLMAYVCI LMGDGHSCDF FLNTALELSE TQGNLLEKCW LSMSKEWWYS 1570 1580 1590 1600 APELTGDQWL QTVLSLPSWD KIVSGNVTLQ DVQKNKFLMR VNILDNPF

References

[1]	"Cytosolic adenylyl cyclase defines a unique signaling molecule in mammals." Buck J., Sinclair M.L., Schapal L., Cann M.J., Levin L.R. Proc. Natl. Acad. Sci. U.S.A. 96:79-84(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 60-67; 254-262 AND 402-409, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Strain: Sprague-Dawley. Tissue: Testis.
[2]	"Identification and functional analysis of splice variants of the germ cell soluble adenylyl cyclase." Jaiswal B.S., Conti M. J. Biol. Chem. 276:31698-31708(2001) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING.
[3]	"Compartmentalization of bicarbonate-sensitive adenylyl cyclase in distinct signaling microdomains." Zippin J.H., Chen Y., Nahirney P., Kamenetsky M., Wuttke M.S., Fischman D.A., Levin L.R., Buck J. FASEB J. 17:82-84(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[4]	"Kinetic properties of 'soluble' adenylyl cyclase. Synergism between calcium and bicarbonate." Litvin T.N., Kamenetsky M., Zarifyan A., Buck J., Levin L.R. J. Biol. Chem. 278:15922-15926(2003) [PubMed] [Europe PMC] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF081941 mRNA. Translation: AAD04035.1.
IPI	IPI00331967.
PIR	T17201.
RefSeq	NP_067716.1. NM_021684.1.
UniGene	Rn.42892.
3D structure databases
ProteinModelPortal	Q9Z286.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000004326.
Proteomic databases
PaxDb	Q9Z286.
PRIDE	Q9Z286.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000004326; ENSRNOP00000004326; ENSRNOG00000003153.
GeneID	59320.
KEGG	rno:59320.
UCSC	RGD:708450. rat.
Organism-specific databases
CTD	55811.
RGD	708450. Adcy10.
Phylogenomic databases
eggNOG	COG2114.
GeneTree	ENSGT00390000001322.
HOGENOM	HOG000060149.
HOVERGEN	HBG103835.
KO	K11265.
Enzyme and pathway databases
BRENDA	4.6.1.1. 5301.
Gene expression databases
ArrayExpress	Q9Z286.
Genevestigator	Q9Z286.
Family and domain databases
Gene3D	3.30.70.1230. 2 hits.
InterPro	IPR001054. A/G_cyclase. IPR016577. Adenylate_cylcase_typ10. [Graphical view]
Pfam	PF00211. Guanylate_cyc. 2 hits. [Graphical view]
PIRSF	PIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
SMART	SM00044. CYCc. 1 hit. [Graphical view]
SUPFAM	SSF55073. A/G_cyclase. 2 hits.
PROSITE	PS00452. GUANYLATE_CYCLASE_1. False negative. PS50125. GUANYLATE_CYCLASE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	611901.

Entry information

Entry name

ADCYA_RAT

Accession

Primary (citable) accession number: Q9Z286

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 1, 1999
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program