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Q9Z286

- ADCYA_RAT

UniProt

Q9Z286 - ADCYA_RAT

Protein

Adenylate cyclase type 10

Gene

Adcy10

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor By similarity.By similarity

    Catalytic activityi

    ATP = 3',5'-cyclic AMP + diphosphate.

    Cofactori

    Binds 2 magnesium ions per subunit.By similarity

    Enzyme regulationi

    Activated by manganese or magnesium ions. In the presence of magnesium ions, the enzyme is activated by bicarbonate while in the presence of manganese ions, the enzyme is inhibited by bicarbonate. In the absence of magnesium and bicarbonate, the enzyme is weakly activated by calcium By similarity.By similarity

    Kineticsi

    1. KM=1 mM for ATP-Mn2+2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471Magnesium 1PROSITE-ProRule annotation
    Metal bindingi47 – 471Magnesium 2PROSITE-ProRule annotation
    Metal bindingi48 – 481Magnesium 2; via carbonyl oxygenPROSITE-ProRule annotation
    Metal bindingi99 – 991Magnesium 1PROSITE-ProRule annotation
    Metal bindingi99 – 991Magnesium 2PROSITE-ProRule annotation

    GO - Molecular functioni

    1. adenylate cyclase activity Source: RGD
    2. ATPase binding Source: RGD
    3. ATP binding Source: UniProtKB-KW
    4. magnesium ion binding Source: InterPro
    5. manganese ion binding Source: RGD

    GO - Biological processi

    1. cAMP biosynthetic process Source: RGD
    2. intracellular signal transduction Source: InterPro
    3. positive regulation of apoptotic process Source: RGD
    4. spermatogenesis Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    cAMP biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi4.6.1.1. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenylate cyclase type 10 (EC:4.6.1.1)
    Alternative name(s):
    Germ cell soluble adenylyl cyclase
    Short name:
    sAC
    Testicular soluble adenylyl cyclase
    Gene namesi
    Name:Adcy10
    Synonyms:Sac
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 13

    Organism-specific databases

    RGDi708450. Adcy10.

    Subcellular locationi

    Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmcytoskeleton. Mitochondrion. Cytoplasmperinuclear region. Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity
    Note: Distributed to subcellular compartments containing cAMP targets. Found as a plasma membrane-associated protein, protein concentrated in the perinuclear region and protein colocalized with actin, tubulin or centriole By similarity.By similarity

    GO - Cellular componenti

    1. apical part of cell Source: RGD
    2. axon Source: RGD
    3. basal part of cell Source: RGD
    4. centriole Source: UniProtKB-SubCell
    5. cytoplasm Source: RGD
    6. cytosol Source: RGD
    7. dendrite Source: RGD
    8. growth cone Source: RGD
    9. mitochondrion Source: UniProtKB-SubCell
    10. neuronal cell body Source: RGD
    11. perinuclear region of cytoplasm Source: UniProtKB-SubCell
    12. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 16081608Adenylate cyclase type 10PRO_0000317104Add
    BLAST

    Post-translational modificationi

    Cleavage may occur to generate the active 48 kDa form.

    Proteomic databases

    PaxDbiQ9Z286.
    PRIDEiQ9Z286.

    Expressioni

    Tissue specificityi

    Preferentially expressed in testis.1 Publication

    Gene expression databases

    GenevestigatoriQ9Z286.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000004326.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z286.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini42 – 179138Guanylate cyclase 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini293 – 418126Guanylate cyclase 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
    Contains 2 guanylate cyclase domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2114.
    GeneTreeiENSGT00390000001322.
    HOGENOMiHOG000060149.
    HOVERGENiHBG103835.
    KOiK11265.
    OMAiTCDSVTY.
    OrthoDBiEOG780RM3.
    PhylomeDBiQ9Z286.

    Family and domain databases

    Gene3Di3.30.70.1230. 2 hits.
    InterProiIPR001054. A/G_cyclase.
    IPR016577. Adenylate_cylcase_typ10.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00211. Guanylate_cyc. 2 hits.
    [Graphical view]
    PIRSFiPIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
    SMARTiSM00044. CYCc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF55073. SSF55073. 2 hits.
    PROSITEiPS50125. GUANYLATE_CYCLASE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced.

    Isoform 1 (identifier: Q9Z286-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSARRQELQD RAIVKIAAHL PDLIVYGDFS PERPSVKCFD GVLMFVDISG     50
    FTAMTEKFST AMYMDRGAEQ LVEILNYYIS AIVEKVLIFG GDILKFAGDA 100
    LLALWKVERK QLKNIITVVI KCSLEIHGLF EAKEVEEGLD IRVKIGLAAG 150
    HITMLVFGDE TRNYFLVIGQ AVDDVRLAQN MAQMNDVILS PNCWQLCDRS 200
    MIEIERIPDQ RAVKVSFLKP PPTFNFDEFF AKCMAFMDYY PSGDHKNFLR 250
    LACMLESDPE LELSLQKYVM EIILKQIDDK QLRGYLSELR PVTIVFVNLM 300
    FKEQDKAEVI GSAIQAACVH ITSVLKVFRG QINKVFMFDK GCSFLCVFGF 350
    PGEKAPDEIT HALESAVDIF DFCSQVHKIR TVSIGVASGI VFCGIVGHTV 400
    RHEYTVIGQK VNIAARMMMY YPGIVTCDSV TYDGSNLPAY FFKELPKKVM 450
    KGVADPGPVY QCLGLNEKVM FGMAYLICNR YEGYPLLGRV REIDYFMSTM 500
    KDFLMTNCSR VLMYEGLPGY GKSQVLMEIE YLASQHENHR AVAIALTKIS 550
    FHQNFYTIQI LMANVLGLDT CKHYKERQTN LQNRVKTLLD DKYHCLLNDI 600
    FHVQFPVSRE MSRMSKIRKQ KQLEALFMKI LEQTVREERI IFIIDEAQFV 650
    DVASWAFIEK LIRSMPIFIV MSLCPFPETP CAAANAIMKN RNTTYITLGT 700
    MQPQEIRDKV CVDLSVSSIP RELDSYLVEG SCGIPYYCEE LLKNLDHHRI 750
    LIFQQAEAEE KTNVTWNNLF KYSVKPTEDM YLYTSIAAGQ KEACYLTSGV 800
    RLKNLSPPAS LKEISLVQLD SMSLSHQMLV RCAAIIGLTF TTELLFEILP 850
    CWNMKMMIKA LATLVESNVF DCFRSSKDLQ LALKQNVTTF EVHYRSLSLK 900
    SKEGLAYSEE EQLREMEGEV IECRILRFCR PIMQKTAYEL WLKDQKKVLH 950
    LKCARFLEES AHRCNHCRNR DFIPYHHFIA DIRLNTLDMD TVKKMVKSHG 1000
    FKTEDEVIFS KSEIPRKFKF PENISITETR EKILHFFDNV IIKMRTSQDD 1050
    VIPLESCHCE ELLQIVILPL AQHFVALEEN NKALYYFLEL ASAYLILGDN 1100
    YNAYMYLGEG ERLLKSLTNE DSWSQTFEYA TFYSLKGEIC FNMGQMVLAK 1150
    KMLRKALKLL NRMFPCNLLS LTFQMHIEKN RLSHFMNQHT QEGSLPGKKL 1200
    AQLFLQSSCF SLLWKIYSLN FFFHYKYYGR LAAIMQMNTS LETQNNFQII 1250
    KAFLDFSLYR HLAGYEGVWF KYEILVMEQL LNLPLKGEAF EIMAYAADAL 1300
    GHIKFLTGHL DLAIELGSRA HKMWSLLRNP NKYHMVLCRL SKPLFLKSRY 1350
    KHLVQVLGWL WDLSVTEEHI FSKAFFYFVC LDIMLYSGFI YRTFEECLEF 1400
    IHHNEDNRIL KFQSGLLLGL YSCIAVWYAR LQEWDNFYKF SNRAKTLVTR 1450
    RTPTVLYYEG ISRYMEGQVL HLQKQIEEQA ENAQDSGVEL LKALETLVAQ 1500
    NTTGPVFYPR LYHLMAYVCI LMGDGHSCDF FLNTALELSE TQGNLLEKCW 1550
    LSMSKEWWYS APELTGDQWL QTVLSLPSWD KIVSGNVTLQ DVQKNKFLMR 1600
    VNILDNPF 1608
    Length:1,608
    Mass (Da):185,854
    Last modified:May 1, 1999 - v1
    Checksum:iBEF6D3F60A5D2556
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF081941 mRNA. Translation: AAD04035.1.
    PIRiT17201.
    RefSeqiNP_067716.1. NM_021684.1. [Q9Z286-1]
    XP_006250251.1. XM_006250189.1. [Q9Z286-1]
    UniGeneiRn.42892.

    Genome annotation databases

    EnsembliENSRNOT00000004326; ENSRNOP00000004326; ENSRNOG00000003153. [Q9Z286-1]
    GeneIDi59320.
    KEGGirno:59320.
    UCSCiRGD:708450. rat. [Q9Z286-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF081941 mRNA. Translation: AAD04035.1 .
    PIRi T17201.
    RefSeqi NP_067716.1. NM_021684.1. [Q9Z286-1 ]
    XP_006250251.1. XM_006250189.1. [Q9Z286-1 ]
    UniGenei Rn.42892.

    3D structure databases

    ProteinModelPortali Q9Z286.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000004326.

    Proteomic databases

    PaxDbi Q9Z286.
    PRIDEi Q9Z286.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000004326 ; ENSRNOP00000004326 ; ENSRNOG00000003153 . [Q9Z286-1 ]
    GeneIDi 59320.
    KEGGi rno:59320.
    UCSCi RGD:708450. rat. [Q9Z286-1 ]

    Organism-specific databases

    CTDi 55811.
    RGDi 708450. Adcy10.

    Phylogenomic databases

    eggNOGi COG2114.
    GeneTreei ENSGT00390000001322.
    HOGENOMi HOG000060149.
    HOVERGENi HBG103835.
    KOi K11265.
    OMAi TCDSVTY.
    OrthoDBi EOG780RM3.
    PhylomeDBi Q9Z286.

    Enzyme and pathway databases

    BRENDAi 4.6.1.1. 5301.

    Miscellaneous databases

    NextBioi 611901.
    PROi Q9Z286.

    Gene expression databases

    Genevestigatori Q9Z286.

    Family and domain databases

    Gene3Di 3.30.70.1230. 2 hits.
    InterProi IPR001054. A/G_cyclase.
    IPR016577. Adenylate_cylcase_typ10.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00211. Guanylate_cyc. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
    SMARTi SM00044. CYCc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    SSF55073. SSF55073. 2 hits.
    PROSITEi PS50125. GUANYLATE_CYCLASE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cytosolic adenylyl cyclase defines a unique signaling molecule in mammals."
      Buck J., Sinclair M.L., Schapal L., Cann M.J., Levin L.R.
      Proc. Natl. Acad. Sci. U.S.A. 96:79-84(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 60-67; 254-262 AND 402-409, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: Sprague-Dawley.
      Tissue: Testis.
    2. "Identification and functional analysis of splice variants of the germ cell soluble adenylyl cyclase."
      Jaiswal B.S., Conti M.
      J. Biol. Chem. 276:31698-31708(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING.
    3. "Compartmentalization of bicarbonate-sensitive adenylyl cyclase in distinct signaling microdomains."
      Zippin J.H., Chen Y., Nahirney P., Kamenetsky M., Wuttke M.S., Fischman D.A., Levin L.R., Buck J.
      FASEB J. 17:82-84(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    4. "Kinetic properties of 'soluble' adenylyl cyclase. Synergism between calcium and bicarbonate."
      Litvin T.N., Kamenetsky M., Zarifyan A., Buck J., Levin L.R.
      J. Biol. Chem. 278:15922-15926(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiADCYA_RAT
    AccessioniPrimary (citable) accession number: Q9Z286
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3