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Q9Z286 (ADCYA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenylate cyclase type 10

EC=4.6.1.1
Alternative name(s):
Germ cell soluble adenylyl cyclase
Short name=sAC
Testicular soluble adenylyl cyclase
Gene names
Name:Adcy10
Synonyms:Sac
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1608 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor By similarity.

Catalytic activity

ATP = 3',5'-cyclic AMP + diphosphate.

Cofactor

Binds 2 magnesium ions per subunit By similarity.

Enzyme regulation

Activated by manganese or magnesium ions. In the presence of magnesium ions, the enzyme is activated by bicarbonate while in the presence of manganese ions, the enzyme is inhibited by bicarbonate. In the absence of magnesium and bicarbonate, the enzyme is weakly activated by calcium By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmcytoskeleton. Mitochondrion. Cytoplasmperinuclear region. Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity. Note: Distributed to subcellular compartments containing cAMP targets. Found as a plasma membrane-associated protein, protein concentrated in the perinuclear region and protein colocalized with actin, tubulin or centriole By similarity. Ref.1 Ref.3

Tissue specificity

Preferentially expressed in testis. Ref.1

Post-translational modification

Cleavage may occur to generate the active 48 kDa form.

Sequence similarities

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Contains 2 guanylate cyclase domains.

Biophysicochemical properties

Kinetic parameters:

KM=1 mM for ATP-Mn2+ Ref.1 Ref.4

Ontologies

Keywords
   Biological processcAMP biosynthesis
   Cellular componentCell membrane
Cytoplasm
Cytoskeleton
Membrane
Mitochondrion
   Coding sequence diversityAlternative splicing
   DomainRepeat
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLyase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcAMP biosynthetic process

Inferred from direct assay PubMed 7225326Ref.1. Source: RGD

intracellular signal transduction

Inferred from electronic annotation. Source: InterPro

positive regulation of apoptotic process

Inferred from mutant phenotype PubMed 19336406. Source: RGD

spermatogenesis

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapical part of cell

Inferred from direct assay PubMed 17959750. Source: RGD

axon

Inferred from direct assay PubMed 16964251. Source: RGD

basal part of cell

Inferred from direct assay PubMed 17959750. Source: RGD

centriole

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasm

Inferred from direct assay PubMed 16627466. Source: RGD

cytosol

Inferred from direct assay Ref.1. Source: RGD

dendrite

Inferred from direct assay PubMed 16964251. Source: RGD

growth cone

Inferred from direct assay PubMed 16964251. Source: RGD

mitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

neuronal cell body

Inferred from direct assay PubMed 16964251. Source: RGD

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATPase binding

Inferred from physical interaction PubMed 17959750. Source: RGD

adenylate cyclase activity

Inferred from direct assay PubMed 7225326Ref.1. Source: RGD

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

manganese ion binding

Inferred from direct assay PubMed 7225326. Source: RGD

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced.
Isoform 1 (identifier: Q9Z286-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 16081608Adenylate cyclase type 10
PRO_0000317104

Regions

Domain42 – 179138Guanylate cyclase 1
Domain293 – 418126Guanylate cyclase 2

Sites

Metal binding471Magnesium 1 By similarity
Metal binding471Magnesium 2 By similarity
Metal binding481Magnesium 2; via carbonyl oxygen By similarity
Metal binding991Magnesium 1 By similarity
Metal binding991Magnesium 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: BEF6D3F60A5D2556

FASTA1,608185,854
        10         20         30         40         50         60 
MSARRQELQD RAIVKIAAHL PDLIVYGDFS PERPSVKCFD GVLMFVDISG FTAMTEKFST 

        70         80         90        100        110        120 
AMYMDRGAEQ LVEILNYYIS AIVEKVLIFG GDILKFAGDA LLALWKVERK QLKNIITVVI 

       130        140        150        160        170        180 
KCSLEIHGLF EAKEVEEGLD IRVKIGLAAG HITMLVFGDE TRNYFLVIGQ AVDDVRLAQN 

       190        200        210        220        230        240 
MAQMNDVILS PNCWQLCDRS MIEIERIPDQ RAVKVSFLKP PPTFNFDEFF AKCMAFMDYY 

       250        260        270        280        290        300 
PSGDHKNFLR LACMLESDPE LELSLQKYVM EIILKQIDDK QLRGYLSELR PVTIVFVNLM 

       310        320        330        340        350        360 
FKEQDKAEVI GSAIQAACVH ITSVLKVFRG QINKVFMFDK GCSFLCVFGF PGEKAPDEIT 

       370        380        390        400        410        420 
HALESAVDIF DFCSQVHKIR TVSIGVASGI VFCGIVGHTV RHEYTVIGQK VNIAARMMMY 

       430        440        450        460        470        480 
YPGIVTCDSV TYDGSNLPAY FFKELPKKVM KGVADPGPVY QCLGLNEKVM FGMAYLICNR 

       490        500        510        520        530        540 
YEGYPLLGRV REIDYFMSTM KDFLMTNCSR VLMYEGLPGY GKSQVLMEIE YLASQHENHR 

       550        560        570        580        590        600 
AVAIALTKIS FHQNFYTIQI LMANVLGLDT CKHYKERQTN LQNRVKTLLD DKYHCLLNDI 

       610        620        630        640        650        660 
FHVQFPVSRE MSRMSKIRKQ KQLEALFMKI LEQTVREERI IFIIDEAQFV DVASWAFIEK 

       670        680        690        700        710        720 
LIRSMPIFIV MSLCPFPETP CAAANAIMKN RNTTYITLGT MQPQEIRDKV CVDLSVSSIP 

       730        740        750        760        770        780 
RELDSYLVEG SCGIPYYCEE LLKNLDHHRI LIFQQAEAEE KTNVTWNNLF KYSVKPTEDM 

       790        800        810        820        830        840 
YLYTSIAAGQ KEACYLTSGV RLKNLSPPAS LKEISLVQLD SMSLSHQMLV RCAAIIGLTF 

       850        860        870        880        890        900 
TTELLFEILP CWNMKMMIKA LATLVESNVF DCFRSSKDLQ LALKQNVTTF EVHYRSLSLK 

       910        920        930        940        950        960 
SKEGLAYSEE EQLREMEGEV IECRILRFCR PIMQKTAYEL WLKDQKKVLH LKCARFLEES 

       970        980        990       1000       1010       1020 
AHRCNHCRNR DFIPYHHFIA DIRLNTLDMD TVKKMVKSHG FKTEDEVIFS KSEIPRKFKF 

      1030       1040       1050       1060       1070       1080 
PENISITETR EKILHFFDNV IIKMRTSQDD VIPLESCHCE ELLQIVILPL AQHFVALEEN 

      1090       1100       1110       1120       1130       1140 
NKALYYFLEL ASAYLILGDN YNAYMYLGEG ERLLKSLTNE DSWSQTFEYA TFYSLKGEIC 

      1150       1160       1170       1180       1190       1200 
FNMGQMVLAK KMLRKALKLL NRMFPCNLLS LTFQMHIEKN RLSHFMNQHT QEGSLPGKKL 

      1210       1220       1230       1240       1250       1260 
AQLFLQSSCF SLLWKIYSLN FFFHYKYYGR LAAIMQMNTS LETQNNFQII KAFLDFSLYR 

      1270       1280       1290       1300       1310       1320 
HLAGYEGVWF KYEILVMEQL LNLPLKGEAF EIMAYAADAL GHIKFLTGHL DLAIELGSRA 

      1330       1340       1350       1360       1370       1380 
HKMWSLLRNP NKYHMVLCRL SKPLFLKSRY KHLVQVLGWL WDLSVTEEHI FSKAFFYFVC 

      1390       1400       1410       1420       1430       1440 
LDIMLYSGFI YRTFEECLEF IHHNEDNRIL KFQSGLLLGL YSCIAVWYAR LQEWDNFYKF 

      1450       1460       1470       1480       1490       1500 
SNRAKTLVTR RTPTVLYYEG ISRYMEGQVL HLQKQIEEQA ENAQDSGVEL LKALETLVAQ 

      1510       1520       1530       1540       1550       1560 
NTTGPVFYPR LYHLMAYVCI LMGDGHSCDF FLNTALELSE TQGNLLEKCW LSMSKEWWYS 

      1570       1580       1590       1600 
APELTGDQWL QTVLSLPSWD KIVSGNVTLQ DVQKNKFLMR VNILDNPF 

« Hide

References

[1]"Cytosolic adenylyl cyclase defines a unique signaling molecule in mammals."
Buck J., Sinclair M.L., Schapal L., Cann M.J., Levin L.R.
Proc. Natl. Acad. Sci. U.S.A. 96:79-84(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 60-67; 254-262 AND 402-409, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
Strain: Sprague-Dawley.
Tissue: Testis.
[2]"Identification and functional analysis of splice variants of the germ cell soluble adenylyl cyclase."
Jaiswal B.S., Conti M.
J. Biol. Chem. 276:31698-31708(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING.
[3]"Compartmentalization of bicarbonate-sensitive adenylyl cyclase in distinct signaling microdomains."
Zippin J.H., Chen Y., Nahirney P., Kamenetsky M., Wuttke M.S., Fischman D.A., Levin L.R., Buck J.
FASEB J. 17:82-84(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[4]"Kinetic properties of 'soluble' adenylyl cyclase. Synergism between calcium and bicarbonate."
Litvin T.N., Kamenetsky M., Zarifyan A., Buck J., Levin L.R.
J. Biol. Chem. 278:15922-15926(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF081941 mRNA. Translation: AAD04035.1.
PIRT17201.
RefSeqNP_067716.1. NM_021684.1. [Q9Z286-1]
XP_006250251.1. XM_006250189.1. [Q9Z286-1]
UniGeneRn.42892.

3D structure databases

ProteinModelPortalQ9Z286.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000004326.

Proteomic databases

PaxDbQ9Z286.
PRIDEQ9Z286.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000004326; ENSRNOP00000004326; ENSRNOG00000003153. [Q9Z286-1]
GeneID59320.
KEGGrno:59320.
UCSCRGD:708450. rat. [Q9Z286-1]

Organism-specific databases

CTD55811.
RGD708450. Adcy10.

Phylogenomic databases

eggNOGCOG2114.
GeneTreeENSGT00390000001322.
HOGENOMHOG000060149.
HOVERGENHBG103835.
KOK11265.
OMATCDSVTY.
OrthoDBEOG780RM3.
PhylomeDBQ9Z286.

Enzyme and pathway databases

BRENDA4.6.1.1. 5301.

Gene expression databases

GenevestigatorQ9Z286.

Family and domain databases

Gene3D3.30.70.1230. 2 hits.
InterProIPR001054. A/G_cyclase.
IPR016577. Adenylate_cylcase_typ10.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00211. Guanylate_cyc. 2 hits.
[Graphical view]
PIRSFPIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
SMARTSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
SSF55073. SSF55073. 2 hits.
PROSITEPS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio611901.
PROQ9Z286.

Entry information

Entry nameADCYA_RAT
AccessionPrimary (citable) accession number: Q9Z286
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 1, 1999
Last modified: May 14, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families