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Protein

Adenylate cyclase type 10

Gene

Adcy10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor (By similarity).By similarity

Catalytic activityi

ATP = 3',5'-cyclic AMP + diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Enzyme regulationi

Activated by manganese or magnesium ions. In the presence of magnesium ions, the enzyme is activated by bicarbonate while in the presence of manganese ions, the enzyme is inhibited by bicarbonate. In the absence of magnesium and bicarbonate, the enzyme is weakly activated by calcium (By similarity).By similarity

Kineticsi

  1. KM=1 mM for ATP-Mn2+2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Magnesium 1PROSITE-ProRule annotation
Metal bindingi47 – 471Magnesium 2PROSITE-ProRule annotation
Metal bindingi48 – 481Magnesium 2; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi99 – 991Magnesium 1PROSITE-ProRule annotation
Metal bindingi99 – 991Magnesium 2PROSITE-ProRule annotation

GO - Molecular functioni

  1. adenylate cyclase activity Source: RGD
  2. ATPase binding Source: RGD
  3. ATP binding Source: UniProtKB-KW
  4. magnesium ion binding Source: InterPro
  5. manganese ion binding Source: RGD

GO - Biological processi

  1. cAMP biosynthetic process Source: RGD
  2. intracellular signal transduction Source: InterPro
  3. positive regulation of apoptotic process Source: RGD
  4. spermatogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

cAMP biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi4.6.1.1. 5301.
ReactomeiREACT_342405. Hedgehog 'off' state.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate cyclase type 10 (EC:4.6.1.1)
Alternative name(s):
Germ cell soluble adenylyl cyclase
Short name:
sAC
Testicular soluble adenylyl cyclase
Gene namesi
Name:Adcy10
Synonyms:Sac
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi708450. Adcy10.

Subcellular locationi

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmcytoskeleton. Mitochondrion. Cytoplasmperinuclear region. Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity
Note: Distributed to subcellular compartments containing cAMP targets. Found as a plasma membrane-associated protein, protein concentrated in the perinuclear region and protein colocalized with actin, tubulin or centriole (By similarity).By similarity

GO - Cellular componenti

  1. apical part of cell Source: RGD
  2. axon Source: RGD
  3. basal part of cell Source: RGD
  4. centriole Source: UniProtKB-SubCell
  5. cytoplasm Source: RGD
  6. cytosol Source: RGD
  7. dendrite Source: RGD
  8. growth cone Source: RGD
  9. mitochondrion Source: UniProtKB-SubCell
  10. neuronal cell body Source: RGD
  11. perinuclear region of cytoplasm Source: UniProtKB-SubCell
  12. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16081608Adenylate cyclase type 10PRO_0000317104Add
BLAST

Post-translational modificationi

Cleavage may occur to generate the active 48 kDa form.

Proteomic databases

PaxDbiQ9Z286.
PRIDEiQ9Z286.

Expressioni

Tissue specificityi

Preferentially expressed in testis.1 Publication

Gene expression databases

GenevestigatoriQ9Z286.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004326.

Structurei

3D structure databases

ProteinModelPortaliQ9Z286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 179138Guanylate cyclase 1PROSITE-ProRule annotationAdd
BLAST
Domaini293 – 418126Guanylate cyclase 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 2 guanylate cyclase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2114.
GeneTreeiENSGT00390000001322.
HOGENOMiHOG000060149.
HOVERGENiHBG103835.
InParanoidiQ9Z286.
KOiK11265.
OMAiPLESCQC.
OrthoDBiEOG780RM3.
PhylomeDBiQ9Z286.

Family and domain databases

Gene3Di3.30.70.1230. 2 hits.
InterProiIPR001054. A/G_cyclase.
IPR016577. Adenylate_cylcase_typ10.
IPR029787. Nucleotide_cyclase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR16305:SF23. PTHR16305:SF23. 1 hit.
PfamiPF00211. Guanylate_cyc. 2 hits.
[Graphical view]
PIRSFiPIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF55073. SSF55073. 2 hits.
PROSITEiPS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.

Isoform 1 (identifier: Q9Z286-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSARRQELQD RAIVKIAAHL PDLIVYGDFS PERPSVKCFD GVLMFVDISG
60 70 80 90 100
FTAMTEKFST AMYMDRGAEQ LVEILNYYIS AIVEKVLIFG GDILKFAGDA
110 120 130 140 150
LLALWKVERK QLKNIITVVI KCSLEIHGLF EAKEVEEGLD IRVKIGLAAG
160 170 180 190 200
HITMLVFGDE TRNYFLVIGQ AVDDVRLAQN MAQMNDVILS PNCWQLCDRS
210 220 230 240 250
MIEIERIPDQ RAVKVSFLKP PPTFNFDEFF AKCMAFMDYY PSGDHKNFLR
260 270 280 290 300
LACMLESDPE LELSLQKYVM EIILKQIDDK QLRGYLSELR PVTIVFVNLM
310 320 330 340 350
FKEQDKAEVI GSAIQAACVH ITSVLKVFRG QINKVFMFDK GCSFLCVFGF
360 370 380 390 400
PGEKAPDEIT HALESAVDIF DFCSQVHKIR TVSIGVASGI VFCGIVGHTV
410 420 430 440 450
RHEYTVIGQK VNIAARMMMY YPGIVTCDSV TYDGSNLPAY FFKELPKKVM
460 470 480 490 500
KGVADPGPVY QCLGLNEKVM FGMAYLICNR YEGYPLLGRV REIDYFMSTM
510 520 530 540 550
KDFLMTNCSR VLMYEGLPGY GKSQVLMEIE YLASQHENHR AVAIALTKIS
560 570 580 590 600
FHQNFYTIQI LMANVLGLDT CKHYKERQTN LQNRVKTLLD DKYHCLLNDI
610 620 630 640 650
FHVQFPVSRE MSRMSKIRKQ KQLEALFMKI LEQTVREERI IFIIDEAQFV
660 670 680 690 700
DVASWAFIEK LIRSMPIFIV MSLCPFPETP CAAANAIMKN RNTTYITLGT
710 720 730 740 750
MQPQEIRDKV CVDLSVSSIP RELDSYLVEG SCGIPYYCEE LLKNLDHHRI
760 770 780 790 800
LIFQQAEAEE KTNVTWNNLF KYSVKPTEDM YLYTSIAAGQ KEACYLTSGV
810 820 830 840 850
RLKNLSPPAS LKEISLVQLD SMSLSHQMLV RCAAIIGLTF TTELLFEILP
860 870 880 890 900
CWNMKMMIKA LATLVESNVF DCFRSSKDLQ LALKQNVTTF EVHYRSLSLK
910 920 930 940 950
SKEGLAYSEE EQLREMEGEV IECRILRFCR PIMQKTAYEL WLKDQKKVLH
960 970 980 990 1000
LKCARFLEES AHRCNHCRNR DFIPYHHFIA DIRLNTLDMD TVKKMVKSHG
1010 1020 1030 1040 1050
FKTEDEVIFS KSEIPRKFKF PENISITETR EKILHFFDNV IIKMRTSQDD
1060 1070 1080 1090 1100
VIPLESCHCE ELLQIVILPL AQHFVALEEN NKALYYFLEL ASAYLILGDN
1110 1120 1130 1140 1150
YNAYMYLGEG ERLLKSLTNE DSWSQTFEYA TFYSLKGEIC FNMGQMVLAK
1160 1170 1180 1190 1200
KMLRKALKLL NRMFPCNLLS LTFQMHIEKN RLSHFMNQHT QEGSLPGKKL
1210 1220 1230 1240 1250
AQLFLQSSCF SLLWKIYSLN FFFHYKYYGR LAAIMQMNTS LETQNNFQII
1260 1270 1280 1290 1300
KAFLDFSLYR HLAGYEGVWF KYEILVMEQL LNLPLKGEAF EIMAYAADAL
1310 1320 1330 1340 1350
GHIKFLTGHL DLAIELGSRA HKMWSLLRNP NKYHMVLCRL SKPLFLKSRY
1360 1370 1380 1390 1400
KHLVQVLGWL WDLSVTEEHI FSKAFFYFVC LDIMLYSGFI YRTFEECLEF
1410 1420 1430 1440 1450
IHHNEDNRIL KFQSGLLLGL YSCIAVWYAR LQEWDNFYKF SNRAKTLVTR
1460 1470 1480 1490 1500
RTPTVLYYEG ISRYMEGQVL HLQKQIEEQA ENAQDSGVEL LKALETLVAQ
1510 1520 1530 1540 1550
NTTGPVFYPR LYHLMAYVCI LMGDGHSCDF FLNTALELSE TQGNLLEKCW
1560 1570 1580 1590 1600
LSMSKEWWYS APELTGDQWL QTVLSLPSWD KIVSGNVTLQ DVQKNKFLMR

VNILDNPF
Length:1,608
Mass (Da):185,854
Last modified:April 30, 1999 - v1
Checksum:iBEF6D3F60A5D2556
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF081941 mRNA. Translation: AAD04035.1.
PIRiT17201.
RefSeqiNP_067716.1. NM_021684.1. [Q9Z286-1]
XP_008767899.1. XM_008769677.1. [Q9Z286-1]
XP_008767900.1. XM_008769678.1. [Q9Z286-1]
XP_008767901.1. XM_008769679.1. [Q9Z286-1]
UniGeneiRn.42892.

Genome annotation databases

EnsembliENSRNOT00000004326; ENSRNOP00000004326; ENSRNOG00000003153. [Q9Z286-1]
GeneIDi59320.
KEGGirno:59320.
UCSCiRGD:708450. rat. [Q9Z286-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF081941 mRNA. Translation: AAD04035.1.
PIRiT17201.
RefSeqiNP_067716.1. NM_021684.1. [Q9Z286-1]
XP_008767899.1. XM_008769677.1. [Q9Z286-1]
XP_008767900.1. XM_008769678.1. [Q9Z286-1]
XP_008767901.1. XM_008769679.1. [Q9Z286-1]
UniGeneiRn.42892.

3D structure databases

ProteinModelPortaliQ9Z286.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004326.

Proteomic databases

PaxDbiQ9Z286.
PRIDEiQ9Z286.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004326; ENSRNOP00000004326; ENSRNOG00000003153. [Q9Z286-1]
GeneIDi59320.
KEGGirno:59320.
UCSCiRGD:708450. rat. [Q9Z286-1]

Organism-specific databases

CTDi55811.
RGDi708450. Adcy10.

Phylogenomic databases

eggNOGiCOG2114.
GeneTreeiENSGT00390000001322.
HOGENOMiHOG000060149.
HOVERGENiHBG103835.
InParanoidiQ9Z286.
KOiK11265.
OMAiPLESCQC.
OrthoDBiEOG780RM3.
PhylomeDBiQ9Z286.

Enzyme and pathway databases

BRENDAi4.6.1.1. 5301.
ReactomeiREACT_342405. Hedgehog 'off' state.

Miscellaneous databases

NextBioi611901.
PROiQ9Z286.

Gene expression databases

GenevestigatoriQ9Z286.

Family and domain databases

Gene3Di3.30.70.1230. 2 hits.
InterProiIPR001054. A/G_cyclase.
IPR016577. Adenylate_cylcase_typ10.
IPR029787. Nucleotide_cyclase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR16305:SF23. PTHR16305:SF23. 1 hit.
PfamiPF00211. Guanylate_cyc. 2 hits.
[Graphical view]
PIRSFiPIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF55073. SSF55073. 2 hits.
PROSITEiPS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cytosolic adenylyl cyclase defines a unique signaling molecule in mammals."
    Buck J., Sinclair M.L., Schapal L., Cann M.J., Levin L.R.
    Proc. Natl. Acad. Sci. U.S.A. 96:79-84(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 60-67; 254-262 AND 402-409, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. "Identification and functional analysis of splice variants of the germ cell soluble adenylyl cyclase."
    Jaiswal B.S., Conti M.
    J. Biol. Chem. 276:31698-31708(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING.
  3. "Compartmentalization of bicarbonate-sensitive adenylyl cyclase in distinct signaling microdomains."
    Zippin J.H., Chen Y., Nahirney P., Kamenetsky M., Wuttke M.S., Fischman D.A., Levin L.R., Buck J.
    FASEB J. 17:82-84(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. "Kinetic properties of 'soluble' adenylyl cyclase. Synergism between calcium and bicarbonate."
    Litvin T.N., Kamenetsky M., Zarifyan A., Buck J., Levin L.R.
    J. Biol. Chem. 278:15922-15926(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiADCYA_RAT
AccessioniPrimary (citable) accession number: Q9Z286
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 4, 2008
Last sequence update: April 30, 1999
Last modified: March 31, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.