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Protein

Adenylate cyclase type 10

Gene

Adcy10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor (By similarity).By similarity

Catalytic activityi

ATP = 3',5'-cyclic AMP + diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Enzyme regulationi

Activated by manganese or magnesium ions. In the presence of magnesium ions, the enzyme is activated by bicarbonate while in the presence of manganese ions, the enzyme is inhibited by bicarbonate. In the absence of magnesium and bicarbonate, the enzyme is weakly activated by calcium (By similarity).By similarity

Kineticsi

  1. KM=1 mM for ATP-Mn2+2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471Magnesium 1PROSITE-ProRule annotation
    Metal bindingi47 – 471Magnesium 2PROSITE-ProRule annotation
    Metal bindingi48 – 481Magnesium 2; via carbonyl oxygenPROSITE-ProRule annotation
    Metal bindingi99 – 991Magnesium 1PROSITE-ProRule annotation
    Metal bindingi99 – 991Magnesium 2PROSITE-ProRule annotation

    GO - Molecular functioni

    • adenylate cyclase activity Source: RGD
    • ATPase binding Source: RGD
    • ATP binding Source: UniProtKB-KW
    • magnesium ion binding Source: InterPro
    • manganese ion binding Source: RGD

    GO - Biological processi

    • cAMP biosynthetic process Source: RGD
    • intracellular signal transduction Source: InterPro
    • positive regulation of apoptotic process Source: RGD
    • spermatogenesis Source: InterPro
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    cAMP biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi4.6.1.1. 5301.
    ReactomeiREACT_342405. Hedgehog 'off' state.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenylate cyclase type 10 (EC:4.6.1.1)
    Alternative name(s):
    Germ cell soluble adenylyl cyclase
    Short name:
    sAC
    Testicular soluble adenylyl cyclase
    Gene namesi
    Name:Adcy10
    Synonyms:Sac
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494 Componenti: Unassembled WGS sequence

    Organism-specific databases

    RGDi708450. Adcy10.

    Subcellular locationi

    GO - Cellular componenti

    • apical part of cell Source: RGD
    • axon Source: RGD
    • basal part of cell Source: RGD
    • centriole Source: UniProtKB-SubCell
    • cytoplasm Source: RGD
    • cytosol Source: RGD
    • dendrite Source: RGD
    • growth cone Source: RGD
    • mitochondrion Source: UniProtKB-SubCell
    • neuronal cell body Source: RGD
    • perinuclear region of cytoplasm Source: UniProtKB-SubCell
    • plasma membrane Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 16081608Adenylate cyclase type 10PRO_0000317104Add
    BLAST

    Post-translational modificationi

    Cleavage may occur to generate the active 48 kDa form.

    Proteomic databases

    PaxDbiQ9Z286.
    PRIDEiQ9Z286.

    Expressioni

    Tissue specificityi

    Preferentially expressed in testis.1 Publication

    Gene expression databases

    GenevisibleiQ9Z286. RN.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000004326.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z286.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini42 – 179138Guanylate cyclase 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini293 – 418126Guanylate cyclase 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
    Contains 2 guanylate cyclase domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2114.
    GeneTreeiENSGT00390000001322.
    HOGENOMiHOG000060149.
    HOVERGENiHBG103835.
    InParanoidiQ9Z286.
    KOiK11265.
    OMAiPLESCQC.
    OrthoDBiEOG780RM3.
    PhylomeDBiQ9Z286.

    Family and domain databases

    Gene3Di3.30.70.1230. 2 hits.
    InterProiIPR001054. A/G_cyclase.
    IPR016577. Adenylate_cylcase_typ10.
    IPR029787. Nucleotide_cyclase.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR16305:SF23. PTHR16305:SF23. 1 hit.
    PfamiPF00211. Guanylate_cyc. 2 hits.
    [Graphical view]
    PIRSFiPIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
    SMARTiSM00044. CYCc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF55073. SSF55073. 2 hits.
    PROSITEiPS50125. GUANYLATE_CYCLASE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced.

    Isoform 1 (identifier: Q9Z286-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MSARRQELQD RAIVKIAAHL PDLIVYGDFS PERPSVKCFD GVLMFVDISG
    60 70 80 90 100
    FTAMTEKFST AMYMDRGAEQ LVEILNYYIS AIVEKVLIFG GDILKFAGDA
    110 120 130 140 150
    LLALWKVERK QLKNIITVVI KCSLEIHGLF EAKEVEEGLD IRVKIGLAAG
    160 170 180 190 200
    HITMLVFGDE TRNYFLVIGQ AVDDVRLAQN MAQMNDVILS PNCWQLCDRS
    210 220 230 240 250
    MIEIERIPDQ RAVKVSFLKP PPTFNFDEFF AKCMAFMDYY PSGDHKNFLR
    260 270 280 290 300
    LACMLESDPE LELSLQKYVM EIILKQIDDK QLRGYLSELR PVTIVFVNLM
    310 320 330 340 350
    FKEQDKAEVI GSAIQAACVH ITSVLKVFRG QINKVFMFDK GCSFLCVFGF
    360 370 380 390 400
    PGEKAPDEIT HALESAVDIF DFCSQVHKIR TVSIGVASGI VFCGIVGHTV
    410 420 430 440 450
    RHEYTVIGQK VNIAARMMMY YPGIVTCDSV TYDGSNLPAY FFKELPKKVM
    460 470 480 490 500
    KGVADPGPVY QCLGLNEKVM FGMAYLICNR YEGYPLLGRV REIDYFMSTM
    510 520 530 540 550
    KDFLMTNCSR VLMYEGLPGY GKSQVLMEIE YLASQHENHR AVAIALTKIS
    560 570 580 590 600
    FHQNFYTIQI LMANVLGLDT CKHYKERQTN LQNRVKTLLD DKYHCLLNDI
    610 620 630 640 650
    FHVQFPVSRE MSRMSKIRKQ KQLEALFMKI LEQTVREERI IFIIDEAQFV
    660 670 680 690 700
    DVASWAFIEK LIRSMPIFIV MSLCPFPETP CAAANAIMKN RNTTYITLGT
    710 720 730 740 750
    MQPQEIRDKV CVDLSVSSIP RELDSYLVEG SCGIPYYCEE LLKNLDHHRI
    760 770 780 790 800
    LIFQQAEAEE KTNVTWNNLF KYSVKPTEDM YLYTSIAAGQ KEACYLTSGV
    810 820 830 840 850
    RLKNLSPPAS LKEISLVQLD SMSLSHQMLV RCAAIIGLTF TTELLFEILP
    860 870 880 890 900
    CWNMKMMIKA LATLVESNVF DCFRSSKDLQ LALKQNVTTF EVHYRSLSLK
    910 920 930 940 950
    SKEGLAYSEE EQLREMEGEV IECRILRFCR PIMQKTAYEL WLKDQKKVLH
    960 970 980 990 1000
    LKCARFLEES AHRCNHCRNR DFIPYHHFIA DIRLNTLDMD TVKKMVKSHG
    1010 1020 1030 1040 1050
    FKTEDEVIFS KSEIPRKFKF PENISITETR EKILHFFDNV IIKMRTSQDD
    1060 1070 1080 1090 1100
    VIPLESCHCE ELLQIVILPL AQHFVALEEN NKALYYFLEL ASAYLILGDN
    1110 1120 1130 1140 1150
    YNAYMYLGEG ERLLKSLTNE DSWSQTFEYA TFYSLKGEIC FNMGQMVLAK
    1160 1170 1180 1190 1200
    KMLRKALKLL NRMFPCNLLS LTFQMHIEKN RLSHFMNQHT QEGSLPGKKL
    1210 1220 1230 1240 1250
    AQLFLQSSCF SLLWKIYSLN FFFHYKYYGR LAAIMQMNTS LETQNNFQII
    1260 1270 1280 1290 1300
    KAFLDFSLYR HLAGYEGVWF KYEILVMEQL LNLPLKGEAF EIMAYAADAL
    1310 1320 1330 1340 1350
    GHIKFLTGHL DLAIELGSRA HKMWSLLRNP NKYHMVLCRL SKPLFLKSRY
    1360 1370 1380 1390 1400
    KHLVQVLGWL WDLSVTEEHI FSKAFFYFVC LDIMLYSGFI YRTFEECLEF
    1410 1420 1430 1440 1450
    IHHNEDNRIL KFQSGLLLGL YSCIAVWYAR LQEWDNFYKF SNRAKTLVTR
    1460 1470 1480 1490 1500
    RTPTVLYYEG ISRYMEGQVL HLQKQIEEQA ENAQDSGVEL LKALETLVAQ
    1510 1520 1530 1540 1550
    NTTGPVFYPR LYHLMAYVCI LMGDGHSCDF FLNTALELSE TQGNLLEKCW
    1560 1570 1580 1590 1600
    LSMSKEWWYS APELTGDQWL QTVLSLPSWD KIVSGNVTLQ DVQKNKFLMR

    VNILDNPF
    Length:1,608
    Mass (Da):185,854
    Last modified:May 1, 1999 - v1
    Checksum:iBEF6D3F60A5D2556
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF081941 mRNA. Translation: AAD04035.1.
    PIRiT17201.
    RefSeqiNP_067716.1. NM_021684.1. [Q9Z286-1]
    XP_008767899.1. XM_008769677.1. [Q9Z286-1]
    XP_008767900.1. XM_008769678.1. [Q9Z286-1]
    XP_008767901.1. XM_008769679.1. [Q9Z286-1]
    UniGeneiRn.42892.

    Genome annotation databases

    EnsembliENSRNOT00000082677; ENSRNOP00000072356; ENSRNOG00000053410. [Q9Z286-1]
    GeneIDi59320.
    KEGGirno:59320.
    UCSCiRGD:708450. rat. [Q9Z286-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF081941 mRNA. Translation: AAD04035.1.
    PIRiT17201.
    RefSeqiNP_067716.1. NM_021684.1. [Q9Z286-1]
    XP_008767899.1. XM_008769677.1. [Q9Z286-1]
    XP_008767900.1. XM_008769678.1. [Q9Z286-1]
    XP_008767901.1. XM_008769679.1. [Q9Z286-1]
    UniGeneiRn.42892.

    3D structure databases

    ProteinModelPortaliQ9Z286.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000004326.

    Proteomic databases

    PaxDbiQ9Z286.
    PRIDEiQ9Z286.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSRNOT00000082677; ENSRNOP00000072356; ENSRNOG00000053410. [Q9Z286-1]
    GeneIDi59320.
    KEGGirno:59320.
    UCSCiRGD:708450. rat. [Q9Z286-1]

    Organism-specific databases

    CTDi55811.
    RGDi708450. Adcy10.

    Phylogenomic databases

    eggNOGiCOG2114.
    GeneTreeiENSGT00390000001322.
    HOGENOMiHOG000060149.
    HOVERGENiHBG103835.
    InParanoidiQ9Z286.
    KOiK11265.
    OMAiPLESCQC.
    OrthoDBiEOG780RM3.
    PhylomeDBiQ9Z286.

    Enzyme and pathway databases

    BRENDAi4.6.1.1. 5301.
    ReactomeiREACT_342405. Hedgehog 'off' state.

    Miscellaneous databases

    NextBioi611901.
    PROiQ9Z286.

    Gene expression databases

    GenevisibleiQ9Z286. RN.

    Family and domain databases

    Gene3Di3.30.70.1230. 2 hits.
    InterProiIPR001054. A/G_cyclase.
    IPR016577. Adenylate_cylcase_typ10.
    IPR029787. Nucleotide_cyclase.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR16305:SF23. PTHR16305:SF23. 1 hit.
    PfamiPF00211. Guanylate_cyc. 2 hits.
    [Graphical view]
    PIRSFiPIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
    SMARTiSM00044. CYCc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF55073. SSF55073. 2 hits.
    PROSITEiPS50125. GUANYLATE_CYCLASE_2. 2 hits.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    1. "Cytosolic adenylyl cyclase defines a unique signaling molecule in mammals."
      Buck J., Sinclair M.L., Schapal L., Cann M.J., Levin L.R.
      Proc. Natl. Acad. Sci. U.S.A. 96:79-84(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 60-67; 254-262 AND 402-409, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: Sprague-Dawley.
      Tissue: Testis.
    2. "Identification and functional analysis of splice variants of the germ cell soluble adenylyl cyclase."
      Jaiswal B.S., Conti M.
      J. Biol. Chem. 276:31698-31708(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING.
    3. "Compartmentalization of bicarbonate-sensitive adenylyl cyclase in distinct signaling microdomains."
      Zippin J.H., Chen Y., Nahirney P., Kamenetsky M., Wuttke M.S., Fischman D.A., Levin L.R., Buck J.
      FASEB J. 17:82-84(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    4. "Kinetic properties of 'soluble' adenylyl cyclase. Synergism between calcium and bicarbonate."
      Litvin T.N., Kamenetsky M., Zarifyan A., Buck J., Levin L.R.
      J. Biol. Chem. 278:15922-15926(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiADCYA_RAT
    AccessioniPrimary (citable) accession number: Q9Z286
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: May 1, 1999
    Last modified: July 22, 2015
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.