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Q9Z286

- ADCYA_RAT

UniProt

Q9Z286 - ADCYA_RAT

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Protein
Adenylate cyclase type 10
Gene
Adcy10, Sac
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor By similarity.

Catalytic activityi

ATP = 3',5'-cyclic AMP + diphosphate.

Cofactori

Binds 2 magnesium ions per subunit By similarity.

Enzyme regulationi

Activated by manganese or magnesium ions. In the presence of magnesium ions, the enzyme is activated by bicarbonate while in the presence of manganese ions, the enzyme is inhibited by bicarbonate. In the absence of magnesium and bicarbonate, the enzyme is weakly activated by calcium By similarity.

Kineticsi

  1. KM=1 mM for ATP-Mn2+2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Magnesium 1 By similarity
Metal bindingi47 – 471Magnesium 2 By similarity
Metal bindingi48 – 481Magnesium 2; via carbonyl oxygen By similarity
Metal bindingi99 – 991Magnesium 1 By similarity
Metal bindingi99 – 991Magnesium 2 By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATPase binding Source: RGD
  3. adenylate cyclase activity Source: RGD
  4. magnesium ion binding Source: InterPro
  5. manganese ion binding Source: RGD

GO - Biological processi

  1. cAMP biosynthetic process Source: RGD
  2. intracellular signal transduction Source: InterPro
  3. positive regulation of apoptotic process Source: RGD
  4. spermatogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

cAMP biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi4.6.1.1. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate cyclase type 10 (EC:4.6.1.1)
Alternative name(s):
Germ cell soluble adenylyl cyclase
Short name:
sAC
Testicular soluble adenylyl cyclase
Gene namesi
Name:Adcy10
Synonyms:Sac
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 13

Organism-specific databases

RGDi708450. Adcy10.

Subcellular locationi

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmcytoskeleton. Mitochondrion. Cytoplasmperinuclear region. Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity
Note: Distributed to subcellular compartments containing cAMP targets. Found as a plasma membrane-associated protein, protein concentrated in the perinuclear region and protein colocalized with actin, tubulin or centriole By similarity.2 Publications

GO - Cellular componenti

  1. apical part of cell Source: RGD
  2. axon Source: RGD
  3. basal part of cell Source: RGD
  4. centriole Source: UniProtKB-SubCell
  5. cytoplasm Source: RGD
  6. cytosol Source: RGD
  7. dendrite Source: RGD
  8. growth cone Source: RGD
  9. mitochondrion Source: UniProtKB-SubCell
  10. neuronal cell body Source: RGD
  11. perinuclear region of cytoplasm Source: UniProtKB-SubCell
  12. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16081608Adenylate cyclase type 10
PRO_0000317104Add
BLAST

Post-translational modificationi

Cleavage may occur to generate the active 48 kDa form.

Proteomic databases

PaxDbiQ9Z286.
PRIDEiQ9Z286.

Expressioni

Tissue specificityi

Preferentially expressed in testis.1 Publication

Gene expression databases

GenevestigatoriQ9Z286.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004326.

Structurei

3D structure databases

ProteinModelPortaliQ9Z286.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 179138Guanylate cyclase 1
Add
BLAST
Domaini293 – 418126Guanylate cyclase 2
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2114.
GeneTreeiENSGT00390000001322.
HOGENOMiHOG000060149.
HOVERGENiHBG103835.
KOiK11265.
OMAiTCDSVTY.
OrthoDBiEOG780RM3.
PhylomeDBiQ9Z286.

Family and domain databases

Gene3Di3.30.70.1230. 2 hits.
InterProiIPR001054. A/G_cyclase.
IPR016577. Adenylate_cylcase_typ10.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00211. Guanylate_cyc. 2 hits.
[Graphical view]
PIRSFiPIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF55073. SSF55073. 2 hits.
PROSITEiPS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced.

Isoform 1 (identifier: Q9Z286-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSARRQELQD RAIVKIAAHL PDLIVYGDFS PERPSVKCFD GVLMFVDISG     50
FTAMTEKFST AMYMDRGAEQ LVEILNYYIS AIVEKVLIFG GDILKFAGDA 100
LLALWKVERK QLKNIITVVI KCSLEIHGLF EAKEVEEGLD IRVKIGLAAG 150
HITMLVFGDE TRNYFLVIGQ AVDDVRLAQN MAQMNDVILS PNCWQLCDRS 200
MIEIERIPDQ RAVKVSFLKP PPTFNFDEFF AKCMAFMDYY PSGDHKNFLR 250
LACMLESDPE LELSLQKYVM EIILKQIDDK QLRGYLSELR PVTIVFVNLM 300
FKEQDKAEVI GSAIQAACVH ITSVLKVFRG QINKVFMFDK GCSFLCVFGF 350
PGEKAPDEIT HALESAVDIF DFCSQVHKIR TVSIGVASGI VFCGIVGHTV 400
RHEYTVIGQK VNIAARMMMY YPGIVTCDSV TYDGSNLPAY FFKELPKKVM 450
KGVADPGPVY QCLGLNEKVM FGMAYLICNR YEGYPLLGRV REIDYFMSTM 500
KDFLMTNCSR VLMYEGLPGY GKSQVLMEIE YLASQHENHR AVAIALTKIS 550
FHQNFYTIQI LMANVLGLDT CKHYKERQTN LQNRVKTLLD DKYHCLLNDI 600
FHVQFPVSRE MSRMSKIRKQ KQLEALFMKI LEQTVREERI IFIIDEAQFV 650
DVASWAFIEK LIRSMPIFIV MSLCPFPETP CAAANAIMKN RNTTYITLGT 700
MQPQEIRDKV CVDLSVSSIP RELDSYLVEG SCGIPYYCEE LLKNLDHHRI 750
LIFQQAEAEE KTNVTWNNLF KYSVKPTEDM YLYTSIAAGQ KEACYLTSGV 800
RLKNLSPPAS LKEISLVQLD SMSLSHQMLV RCAAIIGLTF TTELLFEILP 850
CWNMKMMIKA LATLVESNVF DCFRSSKDLQ LALKQNVTTF EVHYRSLSLK 900
SKEGLAYSEE EQLREMEGEV IECRILRFCR PIMQKTAYEL WLKDQKKVLH 950
LKCARFLEES AHRCNHCRNR DFIPYHHFIA DIRLNTLDMD TVKKMVKSHG 1000
FKTEDEVIFS KSEIPRKFKF PENISITETR EKILHFFDNV IIKMRTSQDD 1050
VIPLESCHCE ELLQIVILPL AQHFVALEEN NKALYYFLEL ASAYLILGDN 1100
YNAYMYLGEG ERLLKSLTNE DSWSQTFEYA TFYSLKGEIC FNMGQMVLAK 1150
KMLRKALKLL NRMFPCNLLS LTFQMHIEKN RLSHFMNQHT QEGSLPGKKL 1200
AQLFLQSSCF SLLWKIYSLN FFFHYKYYGR LAAIMQMNTS LETQNNFQII 1250
KAFLDFSLYR HLAGYEGVWF KYEILVMEQL LNLPLKGEAF EIMAYAADAL 1300
GHIKFLTGHL DLAIELGSRA HKMWSLLRNP NKYHMVLCRL SKPLFLKSRY 1350
KHLVQVLGWL WDLSVTEEHI FSKAFFYFVC LDIMLYSGFI YRTFEECLEF 1400
IHHNEDNRIL KFQSGLLLGL YSCIAVWYAR LQEWDNFYKF SNRAKTLVTR 1450
RTPTVLYYEG ISRYMEGQVL HLQKQIEEQA ENAQDSGVEL LKALETLVAQ 1500
NTTGPVFYPR LYHLMAYVCI LMGDGHSCDF FLNTALELSE TQGNLLEKCW 1550
LSMSKEWWYS APELTGDQWL QTVLSLPSWD KIVSGNVTLQ DVQKNKFLMR 1600
VNILDNPF 1608
Length:1,608
Mass (Da):185,854
Last modified:May 1, 1999 - v1
Checksum:iBEF6D3F60A5D2556
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF081941 mRNA. Translation: AAD04035.1.
PIRiT17201.
RefSeqiNP_067716.1. NM_021684.1. [Q9Z286-1]
XP_006250251.1. XM_006250189.1. [Q9Z286-1]
UniGeneiRn.42892.

Genome annotation databases

EnsembliENSRNOT00000004326; ENSRNOP00000004326; ENSRNOG00000003153. [Q9Z286-1]
GeneIDi59320.
KEGGirno:59320.
UCSCiRGD:708450. rat. [Q9Z286-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF081941 mRNA. Translation: AAD04035.1 .
PIRi T17201.
RefSeqi NP_067716.1. NM_021684.1. [Q9Z286-1 ]
XP_006250251.1. XM_006250189.1. [Q9Z286-1 ]
UniGenei Rn.42892.

3D structure databases

ProteinModelPortali Q9Z286.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000004326.

Proteomic databases

PaxDbi Q9Z286.
PRIDEi Q9Z286.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000004326 ; ENSRNOP00000004326 ; ENSRNOG00000003153 . [Q9Z286-1 ]
GeneIDi 59320.
KEGGi rno:59320.
UCSCi RGD:708450. rat. [Q9Z286-1 ]

Organism-specific databases

CTDi 55811.
RGDi 708450. Adcy10.

Phylogenomic databases

eggNOGi COG2114.
GeneTreei ENSGT00390000001322.
HOGENOMi HOG000060149.
HOVERGENi HBG103835.
KOi K11265.
OMAi TCDSVTY.
OrthoDBi EOG780RM3.
PhylomeDBi Q9Z286.

Enzyme and pathway databases

BRENDAi 4.6.1.1. 5301.

Miscellaneous databases

NextBioi 611901.
PROi Q9Z286.

Gene expression databases

Genevestigatori Q9Z286.

Family and domain databases

Gene3Di 3.30.70.1230. 2 hits.
InterProi IPR001054. A/G_cyclase.
IPR016577. Adenylate_cylcase_typ10.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00211. Guanylate_cyc. 2 hits.
[Graphical view ]
PIRSFi PIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
SMARTi SM00044. CYCc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF55073. SSF55073. 2 hits.
PROSITEi PS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cytosolic adenylyl cyclase defines a unique signaling molecule in mammals."
    Buck J., Sinclair M.L., Schapal L., Cann M.J., Levin L.R.
    Proc. Natl. Acad. Sci. U.S.A. 96:79-84(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 60-67; 254-262 AND 402-409, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. "Identification and functional analysis of splice variants of the germ cell soluble adenylyl cyclase."
    Jaiswal B.S., Conti M.
    J. Biol. Chem. 276:31698-31708(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING.
  3. "Compartmentalization of bicarbonate-sensitive adenylyl cyclase in distinct signaling microdomains."
    Zippin J.H., Chen Y., Nahirney P., Kamenetsky M., Wuttke M.S., Fischman D.A., Levin L.R., Buck J.
    FASEB J. 17:82-84(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. "Kinetic properties of 'soluble' adenylyl cyclase. Synergism between calcium and bicarbonate."
    Litvin T.N., Kamenetsky M., Zarifyan A., Buck J., Levin L.R.
    J. Biol. Chem. 278:15922-15926(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiADCYA_RAT
AccessioniPrimary (citable) accession number: Q9Z286
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 1, 1999
Last modified: May 14, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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