Q9Z282 (GP132_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable G-protein coupled receptor 132

Alternative name(s):
G2 accumulation protein

Gene names

Name:	Gpr132
Synonyms:	G2a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for an unknown ligand. Activates a G alpha protein, most likely G alpha(q). May be involved in apoptosis. Functions at the G2/M checkpoint to delay mitosis. May serve as a mechanism for T- and B-cells, and other cell types, to slow their proliferation and repair damaged DNA to ensure proper replication. Ref.5
Subcellular location	Cell membrane; Multi-pass membrane protein Probable. Note: Internalized and accumulated in endosomal compartments. LPC triggers the relocalization from the endosomal compartment to the cell surface. Ref.5
Tissue specificity	Highly expressed in hematopoietic tissues rich in lymphocytes like spleen and thymus. Weakly expressed in heart and lung. Highly expressed in infiltrating macrophages within atherosclerotic lesions. Ref.4
Induction	By DNA-damaging agents.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G1/S transition of mitotic cell cycle Inferred from direct assay Ref.1. Source: MGI
Cellular_component	integral to plasma membrane Inferred from sequence or structural similarity Ref.1. Source: MGI
Molecular_function	G-protein coupled receptor activity Inferred from sequence or structural similarity Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Probable G-protein coupled receptor 132		PRO_0000069462
Regions
	Topological domain	1 – 42	42	Extracellular Potential
	Transmembrane	43 – 65	23	Helical; Name=1; Potential
	Topological domain	66 – 76	11	Cytoplasmic Potential
	Transmembrane	77 – 99	23	Helical; Name=2; Potential
	Topological domain	100 – 113	14	Extracellular Potential
	Transmembrane	114 – 135	22	Helical; Name=3; Potential
	Topological domain	136 – 155	20	Cytoplasmic Potential
	Transmembrane	156 – 175	20	Helical; Name=4; Potential
	Topological domain	176 – 198	23	Extracellular Potential
	Transmembrane	199 – 221	23	Helical; Name=5; Potential
	Topological domain	222 – 241	20	Cytoplasmic Potential
	Transmembrane	242 – 261	20	Helical; Name=6; Potential
	Topological domain	262 – 286	25	Extracellular Potential
	Transmembrane	287 – 309	23	Helical; Name=7; Potential
	Topological domain	310 – 382	73	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	11	1	N-linked (GlcNAc...) Potential
	Disulfide bond	112 ↔ 184		By similarity
Experimental info
	Mutagenesis	137	1	R → A: Enhanced surface expression and a lower localization to endosomal vesicles. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9Z282 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: D4F8CE0370CCD610	FASTA	382	42,653
	10 20 30 40 50 60 MRSEPTNAAG NTTLGVTSVL QSTSVPSSET CHVSYEESRV VLVVVYSAVC LLGLPANCLT 70 80 90 100 110 120 AWLTLLQVLQ RNVLAVYLFC LSLCELLYIS TVPLWIIYIQ NQHKWNLGPQ ACKVTAYIFF 130 140 150 160 170 180 CNIYISILLL CCISCDRYMA VVYALESRGH RHQRTAVTIS ACVILLVGLV NYPVFDMKVE 190 200 210 220 230 240 KSFCFEPLRM NSKIAGYHYL RFTFGFAIPL GILAFTNHQI FRSIKLSDSL SAAQKNKVKR 250 260 270 280 290 300 SAIAVVTIFL VCFAPYHVVL LVKAASFSFY QGDMDAVCAF ESRLYTVSMV FLCLSTVNSV 310 320 330 340 350 360 ADPIIYVLGT DHSRQEVSRI HTGWKKWSTK TYVTCSKDSE ETHLPTELSN TYTFPNPAHP 370 380 PGSQPAKLGL LCSPERLPEE LC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A DNA damage and stress inducible G protein-coupled receptor blocks cells in G2/M." Weng Z., Fluckiger A.-C., Nisitani S., Wahl M.I., Le L.Q., Hunter C.A., Fernal A.A., Le Beau M.M., Witte O.N. Proc. Natl. Acad. Sci. U.S.A. 95:12334-12339(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Spleen.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Spleen and Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Expression of G2A, a receptor for lysophosphatidylcholine, by macrophages in murine, rabbit, and human atherosclerotic plaques." Rikitake Y., Hirata K., Yamashita T., Iwai K., Kobayashi S., Itoh H., Ozaki M., Ejiri J., Shiomi M., Inoue N., Kawashima S., Yokoyama M. Arterioscler. Thromb. Vasc. Biol. 22:2049-2053(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Lysophosphatidylcholine-induced surface redistribution regulates signaling of the murine G protein-coupled receptor G2A." Wang L., Radu C.G., Yang L.V., Bentolila L.A., Riedinger M., Witte O.N. Mol. Biol. Cell 16:2234-2247(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-137.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF083442 mRNA. Translation: AAC67542.1. AK089866 mRNA. Translation: BAC40980.1. AK153765 mRNA. Translation: BAE32176.1. BC120522 mRNA. Translation: AAI20523.1. BC120524 mRNA. Translation: AAI20525.1.
IPI	IPI00130631.
RefSeq	NP_064309.1. NM_019925.4.
UniGene	Mm.20455.
3D structure databases
ProteinModelPortal	Q9Z282.
SMR	Q9Z282. Positions 35-324.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9Z282.
Proteomic databases
PRIDE	Q9Z282.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000021729; ENSMUSP00000021729; ENSMUSG00000021298.
GeneID	56696.
KEGG	mmu:56696.
UCSC	uc007pfk.1. mouse.
Organism-specific databases
CTD	29933.
MGI	MGI:1890220. Gpr132.
Phylogenomic databases
eggNOG	NOG148904.
GeneTree	ENSGT00600000084401.
HOGENOM	HOG000013146.
HOVERGEN	HBG104029.
InParanoid	Q0VBS4.
KO	K08426.
OMA	ANCLTAW.
OrthoDB	EOG451DQZ.
Gene expression databases
Bgee	Q9Z282.
CleanEx	MM_GPR132.
Genevestigator	Q9Z282.
GermOnline	ENSMUSG00000021298. Mus musculus.
Family and domain databases
InterPro	IPR005388. G2A_lysphc_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01563. G2ARECEPTOR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	313127.
SOURCE	Search...

Entry information

Entry name

GP132_MOUSE

Accession

Primary (citable) accession number: Q9Z282
Secondary accession number(s): Q0VBS4, Q3U5A4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2003
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program