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Protein

Probable G-protein coupled receptor 132

Gene

Gpr132

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May be a receptor for oxidized free fatty acids derived from linoleic and arachidonic acids such as 9-hydroxyoctadecadienoic acid (9-HODE). Activates a G alpha protein, most likely G alpha(q). May be involved in apoptosis. Functions at the G2/M checkpoint to delay mitosis. May function as a sensor that monitors the oxidative states and mediates appropriate cellular responses such as secretion of paracrine signals and attenuation of proliferation. May mediate ths accumulation of intracellular inositol phosphates at acidic pH through proton-sensing activity (By similarity).By similarity

GO - Molecular functioni

  • G-protein coupled receptor activity Source: MGI

GO - Biological processi

  • G1/S transition of mitotic cell cycle Source: MGI
  • negative regulation of G2/M transition of mitotic cell cycle Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_275529. Class A/1 (Rhodopsin-like receptors).
REACT_347934. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable G-protein coupled receptor 132
Alternative name(s):
G2 accumulation protein
Gene namesi
Name:Gpr132
Synonyms:G2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1890220. Gpr132.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

  • Note: Internalized and accumulated in endosomal compartments. LPC triggers the relocalization from the endosomal compartment to the cell surface.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4242ExtracellularSequence AnalysisAdd
BLAST
Transmembranei43 – 6523Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini66 – 7611CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei77 – 9923Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini100 – 11314ExtracellularSequence AnalysisAdd
BLAST
Transmembranei114 – 13522Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini136 – 15520CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei156 – 17520Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini176 – 19823ExtracellularSequence AnalysisAdd
BLAST
Transmembranei199 – 22123Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini222 – 24120CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei242 – 26120Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini262 – 28625ExtracellularSequence AnalysisAdd
BLAST
Transmembranei287 – 30923Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini310 – 38273CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi137 – 1371R → A: Enhanced surface expression and a lower localization to endosomal vesicles. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382Probable G-protein coupled receptor 132PRO_0000069462Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi11 – 111N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi112 ↔ 184PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ9Z282.

PTM databases

PhosphoSiteiQ9Z282.

Expressioni

Tissue specificityi

Highly expressed in hematopoietic tissues rich in lymphocytes like spleen and thymus. Weakly expressed in heart and lung. Highly expressed in infiltrating macrophages within atherosclerotic lesions.1 Publication

Inductioni

By DNA-damaging agents.

Gene expression databases

BgeeiQ9Z282.
CleanExiMM_GPR132.
GenevisibleiQ9Z282. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021729.

Structurei

3D structure databases

ProteinModelPortaliQ9Z282.
SMRiQ9Z282. Positions 38-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG148904.
GeneTreeiENSGT00760000119001.
HOGENOMiHOG000013146.
HOVERGENiHBG104029.
InParanoidiQ9Z282.
KOiK08426.
OMAiNSVADPI.
OrthoDBiEOG76QFJ8.
PhylomeDBiQ9Z282.
TreeFamiTF331803.

Family and domain databases

InterProiIPR005388. G2A_lysphc_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01563. G2ARECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z282-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSEPTNAAG NTTLGVTSVL QSTSVPSSET CHVSYEESRV VLVVVYSAVC
60 70 80 90 100
LLGLPANCLT AWLTLLQVLQ RNVLAVYLFC LSLCELLYIS TVPLWIIYIQ
110 120 130 140 150
NQHKWNLGPQ ACKVTAYIFF CNIYISILLL CCISCDRYMA VVYALESRGH
160 170 180 190 200
RHQRTAVTIS ACVILLVGLV NYPVFDMKVE KSFCFEPLRM NSKIAGYHYL
210 220 230 240 250
RFTFGFAIPL GILAFTNHQI FRSIKLSDSL SAAQKNKVKR SAIAVVTIFL
260 270 280 290 300
VCFAPYHVVL LVKAASFSFY QGDMDAVCAF ESRLYTVSMV FLCLSTVNSV
310 320 330 340 350
ADPIIYVLGT DHSRQEVSRI HTGWKKWSTK TYVTCSKDSE ETHLPTELSN
360 370 380
TYTFPNPAHP PGSQPAKLGL LCSPERLPEE LC
Length:382
Mass (Da):42,653
Last modified:May 1, 1999 - v1
Checksum:iD4F8CE0370CCD610
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083442 mRNA. Translation: AAC67542.1.
AK089866 mRNA. Translation: BAC40980.1.
AK153765 mRNA. Translation: BAE32176.1.
BC120522 mRNA. Translation: AAI20523.1.
BC120524 mRNA. Translation: AAI20525.1.
CCDSiCCDS26199.1.
RefSeqiNP_064309.1. NM_019925.4.
XP_006516165.1. XM_006516102.2.
XP_006516166.1. XM_006516103.2.
UniGeneiMm.20455.

Genome annotation databases

EnsembliENSMUST00000021729; ENSMUSP00000021729; ENSMUSG00000021298.
GeneIDi56696.
KEGGimmu:56696.
UCSCiuc007pfk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083442 mRNA. Translation: AAC67542.1.
AK089866 mRNA. Translation: BAC40980.1.
AK153765 mRNA. Translation: BAE32176.1.
BC120522 mRNA. Translation: AAI20523.1.
BC120524 mRNA. Translation: AAI20525.1.
CCDSiCCDS26199.1.
RefSeqiNP_064309.1. NM_019925.4.
XP_006516165.1. XM_006516102.2.
XP_006516166.1. XM_006516103.2.
UniGeneiMm.20455.

3D structure databases

ProteinModelPortaliQ9Z282.
SMRiQ9Z282. Positions 38-308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021729.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ9Z282.

Proteomic databases

PRIDEiQ9Z282.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021729; ENSMUSP00000021729; ENSMUSG00000021298.
GeneIDi56696.
KEGGimmu:56696.
UCSCiuc007pfk.1. mouse.

Organism-specific databases

CTDi29933.
MGIiMGI:1890220. Gpr132.

Phylogenomic databases

eggNOGiNOG148904.
GeneTreeiENSGT00760000119001.
HOGENOMiHOG000013146.
HOVERGENiHBG104029.
InParanoidiQ9Z282.
KOiK08426.
OMAiNSVADPI.
OrthoDBiEOG76QFJ8.
PhylomeDBiQ9Z282.
TreeFamiTF331803.

Enzyme and pathway databases

ReactomeiREACT_275529. Class A/1 (Rhodopsin-like receptors).
REACT_347934. G alpha (q) signalling events.

Miscellaneous databases

NextBioi313127.
PROiQ9Z282.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z282.
CleanExiMM_GPR132.
GenevisibleiQ9Z282. MM.

Family and domain databases

InterProiIPR005388. G2A_lysphc_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01563. G2ARECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spleen.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Spleen and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Expression of G2A, a receptor for lysophosphatidylcholine, by macrophages in murine, rabbit, and human atherosclerotic plaques."
    Rikitake Y., Hirata K., Yamashita T., Iwai K., Kobayashi S., Itoh H., Ozaki M., Ejiri J., Shiomi M., Inoue N., Kawashima S., Yokoyama M.
    Arterioscler. Thromb. Vasc. Biol. 22:2049-2053(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Lysophosphatidylcholine-induced surface redistribution regulates signaling of the murine G protein-coupled receptor G2A."
    Wang L., Radu C.G., Yang L.V., Bentolila L.A., Riedinger M., Witte O.N.
    Mol. Biol. Cell 16:2234-2247(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-137.

Entry informationi

Entry nameiGP132_MOUSE
AccessioniPrimary (citable) accession number: Q9Z282
Secondary accession number(s): Q0VBS4, Q3U5A4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: May 1, 1999
Last modified: July 22, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.