Q9Z273 (TULP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tubby-related protein 1

Alternative name(s):
Tubby-like protein 1

Gene names

Name:

Tulp1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for normal development of photoreceptor synapses. Required for normal photoreceptor function and for long-term survival of photoreceptor cells. Interacts with cytoskeleton proteins and may play a role in protein transport in photoreceptor cells. Binds lipids, especially phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate, phosphatidylinositol 3,4-bisphosphate, phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 3,4,5-bisphosphate, phosphatidylserine and phosphatidic acid (in vitro) By similarity. Contribute to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages. Ref.3 Ref.4 Ref.6 Ref.8
Subunit structure	Homodimer Probable. May interact with ABCF1, PSIP1, ZEB1 and HMGB2 Potential. Interacts with F-actin By similarity. Interacts with DNM1. Interacts with TUB. Interacts with TYRO3. Ref.4 Ref.7 Ref.8 Ref.9
Subcellular location	Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Secreted. Cell junction › synapse. Note: Detected at synapses between photoreceptor cells and second-order neurons. Does not have a cleavable signal peptide and is secreted by an alternative pathway. Ref.3 Ref.4 Ref.5
Tissue specificity	Retina specific. Detected in the outer plexiform layer in photoreceptor cells (at protein level). Ref.6
Disruption phenotype	Malformation of photoreceptor synapses, followed by photoreceptor degeneration. Ref.6
Sequence similarities	Belongs to the TUB family.

Ontologies

Keywords
Biological process	Phagocytosis Sensory transduction Vision
Cellular component	Cell junction Cell membrane Cytoplasm Membrane Secreted Synapse
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dendrite development Inferred from mutant phenotype Ref.6. Source: UniProtKB eye photoreceptor cell development Inferred from mutant phenotype Ref.6. Source: UniProtKB phagocytosis Inferred from electronic annotation. Source: UniProtKB-KW photoreceptor cell maintenance Inferred from mutant phenotype Ref.6. Source: UniProtKB phototransduction Inferred from mutant phenotype PubMed 10607826. Source: MGI positive regulation of phagocytosis Inferred from sequence or structural similarity. Source: UniProtKB retina development in camera-type eye Inferred from mutant phenotype PubMed 21052544. Source: MGI
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell photoreceptor inner segment Inferred from direct assay Ref.6. Source: UniProtKB photoreceptor outer segment Inferred from direct assay Ref.6. Source: UniProtKB plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB synapse Inferred from direct assay Ref.6. Source: UniProtKB
Molecular_function	G-protein coupled photoreceptor activity Inferred from mutant phenotype PubMed 10607826. Source: MGI actin filament binding Inferred from sequence or structural similarity. Source: UniProtKB phosphatidylinositol-4,5-bisphosphate binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 543	543	Tubby-related protein 1		PRO_0000186467
Regions
	Compositional bias	118 – 127	10	Poly-Asp
	Compositional bias	128 – 132	5	Poly-Glu
	Compositional bias	246 – 254	9	Poly-Glu

Sequences

	Sequence		Length	Mass (Da)
	Q9Z273 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 0F0D9E4D2A2731E7	FASTA	543	60,336
	10 20 30 40 50 60 MPLQEETLRE VWASDSGHEE DCLSPEPPLR PKQRPAQGQK LRKKKPETPD SLESKPRKAG 70 80 90 100 110 120 AGRRKHEEPP ADSAEPRAAQ TVYAKFLRDP EAKKRDPREN FLVARAPDLG GEENSEEDSD 130 140 150 160 170 180 DDDNDDDEEE EEKKEGKKEK SSLPPKKAPK EREKKAKALG PRGDVGSPDA PRKPLRTKKK 190 200 210 220 230 240 EVGEGTKLRK AKKKGPGETD KDPAGSPAAL RKEFPAAMFL VGEGGAAEKG VKKKGPPKGS 250 260 270 280 290 300 EEEKKEEEEE VEEEVASAVM KNSNQKGRAK GKGKKKVKEE RASSPPVEVG EPREFVLQPA 310 320 330 340 350 360 PQGRAVRCRL TRDKKGMDRG MYPSYFLHLD TEKKVFLLAG RKRKRSKTAN YLISSDPTNL 370 380 390 400 410 420 SRGGENFIGK LRSNLLGNRF TVFDNGQNPQ RGGGGDVGSL RQELAAVVYE TNVLGFRGPR 430 440 450 460 470 480 RMTVIIPGMN SDNERVPIRP RNASDGLLVR WQNKTLESLI ELHNKPPIWN EDSGSYTLNF 490 500 510 520 530 540 QGRVTQASVK NFQIVHADDP DYIVLQFGRV AEDAFTLDYR YPLCALQAFA IALSSFDGKL ACE

References

[1]	"Molecular evolution of the tubby gene family." Naggert J.K., Nishina P.M., Fitch D., McGinnis N., Basson M., Yan G., Cardon L., Shiva N., Duyao M., Ikeda A., McGinnis A., North M.A. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"Apoptotic photoreceptor cell death in tubby mice and the localization of tubby gene family members in the retina." Ikeda S., Sorokina I., Naggert J.K., North M.A., Nishina P.M. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[3]	"A role for the Tubby-like protein 1 in rhodopsin transport." Hagstrom S.A., Adamian M., Scimeca M., Pawlyk B.S., Yue G., Li T. Invest. Ophthalmol. Vis. Sci. 42:1955-1962(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION.
[4]	"Interaction between the photoreceptor-specific tubby-like protein 1 and the neuronal-specific GTPase dynamin-1." Xi Q., Pauer G.J.T., Ball S.L., Rayborn M., Hollyfield J.G., Peachey N.S., Crabb J.W., Hagstrom S.A. Invest. Ophthalmol. Vis. Sci. 48:2837-2844(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DNM1, MASS SPECTROMETRY, SUBCELLULAR LOCATION, SUBUNIT, FUNCTION.
[5]	"Unconventional secretion of tubby and tubby-like protein 1." Caberoy N.B., Li W. FEBS Lett. 583:3057-3062(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Early synaptic defects in tulp1-/- mice." Grossman G.H., Pauer G.J.T., Narendra U., Peachey N.S., Hagstrom S.A. Invest. Ophthalmol. Vis. Sci. 50:3074-3083(2009) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE, FUNCTION, TISSUE SPECIFICITY.
[7]	"Tubby and tubby-like protein 1 are new MerTK ligands for phagocytosis." Caberoy N.B., Zhou Y., Li W. EMBO J. 29:3898-3910(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TYRO3.
[8]	"Identification of tubby and tubby-like protein 1 as eat-me signals by phage display." Caberoy N.B., Maiguel D., Kim Y., Li W. Exp. Cell Res. 316:245-257(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH TUB.
[9]	"Efficient identification of tubby-binding proteins by an improved system of T7 phage display." Caberoy N.B., Zhou Y., Jiang X., Alvarado G., Li W. J. Mol. Recognit. 23:74-83(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HMGB2; ABCF1; PSIP1 AND ZEB1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF085681 mRNA. Translation: AAD13757.1. AF105711 mRNA. Translation: AAD38451.1.
IPI	IPI00130590.
RefSeq	NP_067453.1. NM_021478.1.
UniGene	Mm.42102.
3D structure databases
ProteinModelPortal	Q9Z273.
SMR	Q9Z273. Positions 292-537.
ModBase	Search...
PTM databases
PhosphoSite	Q9Z273.
Proteomic databases
PRIDE	Q9Z273.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000041819; ENSMUSP00000049070; ENSMUSG00000037446.
GeneID	22157.
KEGG	mmu:22157.
UCSC	uc008bqv.1. mouse.
Organism-specific databases
CTD	7287.
MGI	MGI:109571. Tulp1.
Phylogenomic databases
eggNOG	NOG286778.
GeneTree	ENSGT00610000085970.
HOGENOM	HOG000016044.
HOVERGEN	HBG018010.
InParanoid	Q9Z273.
OMA	PESPCPT.
OrthoDB	EOG42821K.
Gene expression databases
ArrayExpress	Q9Z273.
Bgee	Q9Z273.
CleanEx	MM_TULP1.
Genevestigator	Q9Z273.
GermOnline	ENSMUSG00000037446. Mus musculus.
Family and domain databases
Gene3D	3.20.90.10. 1 hit.
InterPro	IPR000007. Tubby_C. IPR025659. Tubby_C-like. IPR018066. Tubby_C_CS. [Graphical view]
Pfam	PF01167. Tub. 1 hit. [Graphical view]
PRINTS	PR01573. SUPERTUBBY.
SUPFAM	SSF54518. Tubby_C. 1 hit.
PROSITE	PS01200. TUB_1. 1 hit. PS01201. TUB_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TULP1. mouse.
NextBio	302082.
SOURCE	Search...

Entry information

Entry name

TULP1_MOUSE

Accession

Primary (citable) accession number: Q9Z273

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	May 1, 1999
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program