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Q9Z272 (GIT1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ARF GTPase-activating protein GIT1
Short name=ARF GAP GIT1
Alternative name(s):
Cool-associated and tyrosine-phosphorylated protein 1
Short name=CAT-1
Short name=CAT1
G protein-coupled receptor kinase-interactor 1
GRK-interacting protein 1
Gene names
Name:Git1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length770 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.

Subunit structure

Interacts with SCRIB By similarity. Interacts with G protein-coupled receptor kinases: ADRBK1/GRK2, PPFIA1 and PPFIA4, with ARHGEF6/alpha-PIX, with ARHGEF7/beta-PIX, with PXN/paxillin and with PTK2/FAK1. Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and PAK1. Interacts with TGFB1I1. Ref.1 Ref.2 Ref.3 Ref.4

Subcellular location

Cytoplasm By similarity. Note: Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin By similarity.

Tissue specificity

Expressed in numerous tissues, with highest levels in testis. Ref.1

Post-translational modification

Phosphorylated on tyrosine residues by PTK2/FAK1 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase By similarity.

Sequence similarities

Contains 3 ANK repeats.

Contains 1 Arf-GAP domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Arhgef7Q9ES282EBI-3842379,EBI-642580From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 770770ARF GTPase-activating protein GIT1
PRO_0000074202

Regions

Domain1 – 124124Arf-GAP
Repeat132 – 16130ANK 1
Repeat166 – 19530ANK 2
Repeat199 – 22830ANK 3
Zinc finger11 – 3424C4-type
Region253 – 424172PTK2/FAK1-binding
Region254 – 376123ARHGEF6-binding
Region646 – 770125Interaction with PXN and TGFB1I1

Amino acid modifications

Modified residue2241Phosphotyrosine By similarity
Modified residue3701Phosphoserine By similarity
Modified residue3711Phosphoserine By similarity
Modified residue3731Phosphothreonine By similarity
Modified residue3841Phosphoserine By similarity
Modified residue3921Phosphotyrosine By similarity
Modified residue3941Phosphoserine By similarity
Modified residue3971Phosphoserine By similarity
Modified residue4011Phosphothreonine By similarity
Modified residue4191Phosphoserine By similarity
Modified residue5171Phosphoserine By similarity
Modified residue5541Phosphotyrosine By similarity
Modified residue5631Phosphotyrosine By similarity
Modified residue6011Phosphoserine By similarity
Modified residue6051Phosphoserine By similarity
Modified residue6101Phosphothreonine By similarity

Secondary structure

1 ............... 770
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9Z272 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 797C8EB7FCB3F481

FASTA77085,231
        10         20         30         40         50         60 
MSRKGPRAEV CADCSAPDPG WASISRGVLV CDECCSVHRS LGRHISIVKH LRHSAWPPTL 

        70         80         90        100        110        120 
LQMVHTLASN GANSIWEHSL LDPAQVQSGR RKANPQDKVH PIKSEFIRAK YQMLAFVHKL 

       130        140        150        160        170        180 
PCRDDDGVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGTTP LHVAAKAGQT 

       190        200        210        220        230        240 
LQAELLVVYG ADPGSPDVNG RTPIDYARQA GHHELAERLV ECQYELTDRL AFYLCGRKPD 

       250        260        270        280        290        300 
HKNGHYIIPQ MADRSRQKCM SQSLDLSELA KAAKKKLQAL SNRLFEELAM DVYDEVDRRE 

       310        320        330        340        350        360 
NDAVWLATQN HSTLVTERSA VPFLPVNPEY SATRNQGRQK LARFNAREFA TLIIDILSEA 

       370        380        390        400        410        420 
KRRQQGKSLS SPTDNLELSA RNQSDLDDQH DYDSVASDED TDQEPLPSAG ATRNNRARSM 

       430        440        450        460        470        480 
DSSDLSDGAV TLQEYLELKK ALATSEAKVQ QLMKVNSSLS DELRKLQREI HKLQAENLQL 

       490        500        510        520        530        540 
RQPPGPVPVP SLPSERAEHT LMGPGGSTHR RDRQAFSMYE PGSALKPFGG APGDELATRL 

       550        560        570        580        590        600 
QPFHSTELED DAIYSVHVPA GLYRIRKGVS ASSVTFTPSS PLLSSSQEGS RHASKLSRHG 

       610        620        630        640        650        660 
SGAESDYENT QSGEPLLGLE GKRFLELSKE DELHAELESL DGDPDPGLPS TEDVILKTEQ 

       670        680        690        700        710        720 
VTKNIQELLR AAQEFKHDSF VPCSEKIHLA VTEMASLFPK RPALEPVRSS LRLLNASAYR 

       730        740        750        760        770 
LQSECRKTVP PEPGAPVDFQ LLTQQVIQCA YDIAKAAKQL VTITTREKKQ

« Hide

References

[1]"Beta2-adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein."
Premont R.T., Claing A., Vitale N., Freeman J.L.R., Pitcher J.A., Patton W.A., Moss J., Vaughan M., Lefkowitz R.J.
Proc. Natl. Acad. Sci. U.S.A. 95:14082-14087(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH ADRBK1, TISSUE SPECIFICITY, CHARACTERIZATION.
[2]"Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal complex disassembly."
Zhao Z.-S., Manser E., Loo T.-H., Lim L.
Mol. Cell. Biol. 20:6354-6363(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARHGEF6; PXN AND PTK2/FAK1.
[3]"Hic-5 interacts with GIT1 with a different binding mode from paxillin."
Nishiya N., Shirai T., Suzuki W., Nose K.
J. Biochem. 132:279-289(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TGFB1I1.
[4]"Interaction between liprin-alpha and GIT1 is required for AMPA receptor targeting."
Ko J., Kim S., Valtschanoff J.G., Shin H., Lee J.R., Sheng M., Premont R.T., Weinberg R.J., Kim E.
J. Neurosci. 23:1667-1677(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PPFIA1 AND PPFIA4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF085693 mRNA. Translation: AAC83348.1.
IPIIPI00209293.
RefSeqNP_114002.1. NM_031814.1.
UniGeneRn.34521.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2JX0NMR-A640-770[»]
2W6AX-ray1.40A/B426-483[»]
ProteinModelPortalQ9Z272.
SMRQ9Z272. Positions 640-770.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9Z272. 1 interaction.
MINTMINT-261281.
STRING10116.ENSRNOP00000020105.

PTM databases

PhosphoSiteQ9Z272.

Proteomic databases

PaxDbQ9Z272.
PRIDEQ9Z272.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID83709.
KEGGrno:83709.

Organism-specific databases

CTD28964.
RGD69331. Git1.

Phylogenomic databases

eggNOGNOG260681.
HOGENOMHOG000232135.
HOVERGENHBG012506.
InParanoidQ9Z272.
KOK05737.
OrthoDBEOG4GTKCB.

Gene expression databases

ArrayExpressQ9Z272.
GenevestigatorQ9Z272.
GermOnlineENSRNOG00000014883. Rattus norvegicus.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR022018. GIT1_C.
IPR013724. GIT_SHD.
[Graphical view]
PfamPF00023. Ank. 1 hit.
PF01412. ArfGap. 1 hit.
PF12205. GIT1_C. 1 hit.
PF08518. GIT_SHD. 2 hits.
[Graphical view]
PRINTSPR00405. REVINTRACTNG.
SMARTSM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00555. GIT. 2 hits.
[Graphical view]
SUPFAMSSF48403. ANK. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9Z272.
NextBio616273.

Entry information

Entry nameGIT1_RAT
AccessionPrimary (citable) accession number: Q9Z272
Entry history
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 1, 1999
Last modified: May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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