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Q9Z272

- GIT1_RAT

UniProt

Q9Z272 - GIT1_RAT

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Protein
ARF GTPase-activating protein GIT1
Gene
Git1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri11 – 3424C4-type
Add
BLAST

GO - Molecular functioni

  1. ARF GTPase activator activity Source: RGD
  2. protein binding Source: IntAct
  3. protein homodimerization activity Source: RGD
  4. small GTPase binding Source: RGD
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. positive regulation of GTPase activity Source: GOC
  2. regulation of ARF GTPase activity Source: InterPro
  3. regulation of G-protein coupled receptor protein signaling pathway Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
ARF GTPase-activating protein GIT1
Short name:
ARF GAP GIT1
Alternative name(s):
Cool-associated and tyrosine-phosphorylated protein 1
Short name:
CAT-1
Short name:
CAT1
G protein-coupled receptor kinase-interactor 1
GRK-interacting protein 1
Gene namesi
Name:Git1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi69331. Git1.

Subcellular locationi

Cytoplasm By similarity
Note: Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. focal adhesion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 770770ARF GTPase-activating protein GIT1
PRO_0000074202Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei224 – 2241Phosphotyrosine By similarity
Modified residuei371 – 3711Phosphoserine By similarity
Modified residuei373 – 3731Phosphothreonine By similarity
Modified residuei384 – 3841Phosphoserine By similarity
Modified residuei392 – 3921Phosphotyrosine By similarity
Modified residuei394 – 3941Phosphoserine By similarity
Modified residuei397 – 3971Phosphoserine By similarity
Modified residuei401 – 4011Phosphothreonine By similarity
Modified residuei419 – 4191Phosphoserine By similarity
Modified residuei554 – 5541Phosphotyrosine By similarity
Modified residuei563 – 5631Phosphotyrosine By similarity
Modified residuei601 – 6011Phosphoserine By similarity
Modified residuei605 – 6051Phosphoserine By similarity
Modified residuei610 – 6101Phosphothreonine By similarity

Post-translational modificationi

Phosphorylated on tyrosine residues by PTK2/FAK1 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9Z272.
PRIDEiQ9Z272.

PTM databases

PhosphoSiteiQ9Z272.

Expressioni

Tissue specificityi

Expressed in numerous tissues, with highest levels in testis.1 Publication

Gene expression databases

GenevestigatoriQ9Z272.

Interactioni

Subunit structurei

Interacts with SCRIB By similarity. Interacts with G protein-coupled receptor kinases: ADRBK1/GRK2, PPFIA1 and PPFIA4, with ARHGEF6/alpha-PIX, with ARHGEF7/beta-PIX, with PXN/paxillin and with PTK2/FAK1. Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and PAK1. Interacts with TGFB1I1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Arhgef7Q9ES282EBI-3842379,EBI-642580From a different organism.

Protein-protein interaction databases

BioGridi249809. 4 interactions.
IntActiQ9Z272. 3 interactions.
MINTiMINT-261281.
STRINGi10116.ENSRNOP00000020105.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi432 – 48150
Beta strandi645 – 6473
Helixi651 – 67323
Helixi677 – 69620
Beta strandi700 – 7023
Helixi705 – 72622
Helixi739 – 76628

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JX0NMR-A640-770[»]
2W6AX-ray1.40A/B426-483[»]
ProteinModelPortaliQ9Z272.
SMRiQ9Z272. Positions 640-770.

Miscellaneous databases

EvolutionaryTraceiQ9Z272.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 124124Arf-GAP
Add
BLAST
Repeati132 – 16130ANK 1
Add
BLAST
Repeati166 – 19530ANK 2
Add
BLAST
Repeati199 – 22830ANK 3
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni253 – 424172PTK2/FAK1-binding
Add
BLAST
Regioni254 – 376123ARHGEF6-binding
Add
BLAST
Regioni646 – 770125Interaction with PXN and TGFB1I1
Add
BLAST

Sequence similaritiesi

Contains 3 ANK repeats.
Contains 1 Arf-GAP domain.

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG260681.
HOGENOMiHOG000232135.
HOVERGENiHBG012506.
InParanoidiQ9Z272.
KOiK05737.
PhylomeDBiQ9Z272.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR022018. GIT1_C.
IPR013724. GIT_SHD.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
PF01412. ArfGap. 1 hit.
PF12205. GIT1_C. 1 hit.
PF08518. GIT_SHD. 2 hits.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00555. GIT. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z272-1 [UniParc]FASTAAdd to Basket

« Hide

MSRKGPRAEV CADCSAPDPG WASISRGVLV CDECCSVHRS LGRHISIVKH    50
LRHSAWPPTL LQMVHTLASN GANSIWEHSL LDPAQVQSGR RKANPQDKVH 100
PIKSEFIRAK YQMLAFVHKL PCRDDDGVTA KDLSKQLHSS VRTGNLETCL 150
RLLSLGAQAN FFHPEKGTTP LHVAAKAGQT LQAELLVVYG ADPGSPDVNG 200
RTPIDYARQA GHHELAERLV ECQYELTDRL AFYLCGRKPD HKNGHYIIPQ 250
MADRSRQKCM SQSLDLSELA KAAKKKLQAL SNRLFEELAM DVYDEVDRRE 300
NDAVWLATQN HSTLVTERSA VPFLPVNPEY SATRNQGRQK LARFNAREFA 350
TLIIDILSEA KRRQQGKSLS SPTDNLELSA RNQSDLDDQH DYDSVASDED 400
TDQEPLPSAG ATRNNRARSM DSSDLSDGAV TLQEYLELKK ALATSEAKVQ 450
QLMKVNSSLS DELRKLQREI HKLQAENLQL RQPPGPVPVP SLPSERAEHT 500
LMGPGGSTHR RDRQAFSMYE PGSALKPFGG APGDELATRL QPFHSTELED 550
DAIYSVHVPA GLYRIRKGVS ASSVTFTPSS PLLSSSQEGS RHASKLSRHG 600
SGAESDYENT QSGEPLLGLE GKRFLELSKE DELHAELESL DGDPDPGLPS 650
TEDVILKTEQ VTKNIQELLR AAQEFKHDSF VPCSEKIHLA VTEMASLFPK 700
RPALEPVRSS LRLLNASAYR LQSECRKTVP PEPGAPVDFQ LLTQQVIQCA 750
YDIAKAAKQL VTITTREKKQ 770
Length:770
Mass (Da):85,231
Last modified:May 1, 1999 - v1
Checksum:i797C8EB7FCB3F481
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF085693 mRNA. Translation: AAC83348.1.
RefSeqiNP_114002.1. NM_031814.1.
UniGeneiRn.34521.

Genome annotation databases

GeneIDi83709.
KEGGirno:83709.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF085693 mRNA. Translation: AAC83348.1 .
RefSeqi NP_114002.1. NM_031814.1.
UniGenei Rn.34521.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2JX0 NMR - A 640-770 [» ]
2W6A X-ray 1.40 A/B 426-483 [» ]
ProteinModelPortali Q9Z272.
SMRi Q9Z272. Positions 640-770.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 249809. 4 interactions.
IntActi Q9Z272. 3 interactions.
MINTi MINT-261281.
STRINGi 10116.ENSRNOP00000020105.

PTM databases

PhosphoSitei Q9Z272.

Proteomic databases

PaxDbi Q9Z272.
PRIDEi Q9Z272.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 83709.
KEGGi rno:83709.

Organism-specific databases

CTDi 28964.
RGDi 69331. Git1.

Phylogenomic databases

eggNOGi NOG260681.
HOGENOMi HOG000232135.
HOVERGENi HBG012506.
InParanoidi Q9Z272.
KOi K05737.
PhylomeDBi Q9Z272.

Miscellaneous databases

EvolutionaryTracei Q9Z272.
NextBioi 616273.
PROi Q9Z272.

Gene expression databases

Genevestigatori Q9Z272.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR022018. GIT1_C.
IPR013724. GIT_SHD.
[Graphical view ]
Pfami PF00023. Ank. 1 hit.
PF01412. ArfGap. 1 hit.
PF12205. GIT1_C. 1 hit.
PF08518. GIT_SHD. 2 hits.
[Graphical view ]
PRINTSi PR00405. REVINTRACTNG.
SMARTi SM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00555. GIT. 2 hits.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 2 hits.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Beta2-adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein."
    Premont R.T., Claing A., Vitale N., Freeman J.L.R., Pitcher J.A., Patton W.A., Moss J., Vaughan M., Lefkowitz R.J.
    Proc. Natl. Acad. Sci. U.S.A. 95:14082-14087(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH ADRBK1, TISSUE SPECIFICITY, CHARACTERIZATION.
  2. "Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal complex disassembly."
    Zhao Z.-S., Manser E., Loo T.-H., Lim L.
    Mol. Cell. Biol. 20:6354-6363(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARHGEF6; PXN AND PTK2/FAK1.
  3. "Hic-5 interacts with GIT1 with a different binding mode from paxillin."
    Nishiya N., Shirai T., Suzuki W., Nose K.
    J. Biochem. 132:279-289(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TGFB1I1.
  4. "Interaction between liprin-alpha and GIT1 is required for AMPA receptor targeting."
    Ko J., Kim S., Valtschanoff J.G., Shin H., Lee J.R., Sheng M., Premont R.T., Weinberg R.J., Kim E.
    J. Neurosci. 23:1667-1677(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PPFIA1 AND PPFIA4.

Entry informationi

Entry nameiGIT1_RAT
AccessioniPrimary (citable) accession number: Q9Z272
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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