Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vesicle-associated membrane protein-associated protein A

Gene

Vapa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to OSBPL3, which mediates recruitment of VAPA to plasma membrane sites (By similarity). The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface (By similarity). With OSBPL3, may regulate ER morphology (By similarity). May play a role in vesicle trafficking (PubMed:19289470).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-RNO-1660661 Sphingolipid de novo biosynthesis
R-RNO-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein-associated protein A
Short name:
VAMP-A
Short name:
VAMP-associated protein A
Short name:
VAP-A
Alternative name(s):
33 kDa VAMP-associated protein
Short name:
VAP-33
Gene namesi
Name:Vapa
Synonyms:Vap33
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi61803 Vapa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 227CytoplasmicSequence analysisAdd BLAST226
Transmembranei228 – 248Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell junction, Cell membrane, Endoplasmic reticulum, Membrane, Nucleus, Tight junction

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi94K → D: Loss of interaction with OSBPL1A; when associated with D-96. 1 Publication1
Mutagenesisi96M → D: Loss of interaction with OSBPL1A; when associated with D-94. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002134722 – 249Vesicle-associated membrane protein-associated protein AAdd BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei125N6-acetyllysineBy similarity1
Modified residuei166PhosphoserineBy similarity1
Modified residuei170PhosphothreonineBy similarity1
Modified residuei214PhosphoserineCombined sources1
Modified residuei216PhosphoserineBy similarity1
Modified residuei219PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9Z270
PRIDEiQ9Z270

PTM databases

iPTMnetiQ9Z270
PhosphoSitePlusiQ9Z270
SwissPalmiQ9Z270

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSRNOG00000014765
ExpressionAtlasiQ9Z270 baseline
GenevisibleiQ9Z270 RN

Interactioni

Subunit structurei

Homodimer, and heterodimer with VAPB. Interacts with VAMP1, VAMP2, STX1A, BET1, SEC22C and with the C-terminal domain of OCLN (By similarity). Interacts with OSBP (By similarity). Interacts (via C-terminus) with RSAD2/viperin (via C-terminus) (By similarity). Interacts with OSBPL1A (PubMed:16004875). Interacts (via MSP domain) with ZFYVE27; may retain ZFYVE27 in the endoplasmic reticulum and regulate its function in cell projections formation (PubMed:19289470). Interacts with OSBPL3 (phosphorylated form). Interacts with KIF5A in a ZFYVE27-dependent manner (By similarity). Interacts with STARD3 (via FFAT motif) (By similarity). Interacts with STARD3NL (via FFAT motif) (By similarity).By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • cadherin binding Source: RGD
  • FFAT motif binding Source: RGD
  • identical protein binding Source: IntAct
  • microtubule binding Source: RGD
  • protein domain specific binding Source: RGD
  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

BioGridi248655, 1 interactor
DIPiDIP-48448N
IntActiQ9Z270, 3 interactors
MINTiQ9Z270
STRINGi10116.ENSRNOP00000020681

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi15 – 27Combined sources13
Beta strandi33 – 40Combined sources8
Beta strandi43 – 45Combined sources3
Beta strandi47 – 54Combined sources8
Helixi56 – 58Combined sources3
Beta strandi59 – 63Combined sources5
Beta strandi65 – 68Combined sources4
Beta strandi73 – 80Combined sources8
Beta strandi94 – 101Combined sources8
Helixi109 – 114Combined sources6
Helixi118 – 120Combined sources3
Beta strandi122 – 131Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z9LX-ray1.70A8-132[»]
1Z9OX-ray1.90A/B/C/D/E/F8-132[»]
ProteinModelPortaliQ9Z270
SMRiQ9Z270
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Z270

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 131MSPPROSITE-ProRule annotationAdd BLAST118

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili168 – 207Sequence analysisAdd BLAST40

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0439 Eukaryota
COG5066 LUCA
GeneTreeiENSGT00390000006947
HOGENOMiHOG000293182
HOVERGENiHBG028551
InParanoidiQ9Z270
KOiK06096
OMAiTTQVEIC
PhylomeDBiQ9Z270
TreeFamiTF317024

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR013783 Ig-like_fold
IPR000535 MSP_dom
IPR008962 PapD-like_sf
IPR016763 VAP
IPR030229 VAPA
PANTHERiPTHR10809 PTHR10809, 1 hit
PTHR10809:SF40 PTHR10809:SF40, 1 hit
PfamiView protein in Pfam
PF00635 Motile_Sperm, 1 hit
PIRSFiPIRSF019693 VAMP-associated, 1 hit
SUPFAMiSSF49354 SSF49354, 1 hit
PROSITEiView protein in PROSITE
PS50202 MSP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z270-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASASGAMAK HEQILVLDPP SDLKFKGPFT DVVTTNLKLQ NPSDRKVCFK
60 70 80 90 100
VKTTAPRRYC VRPNSGVIDP GSIVTVSVML QPFDYDPNEK SKHKFMVQTI
110 120 130 140 150
FAPPNISDME AVWKEAKPDE LMDSKLRCVF EMPNENDKLN DMEPSKAVPL
160 170 180 190 200
NASKQDGPLP KPHSVSLNDT ETRKLMEECK RLQGEMMKLS EENRHLRDEG
210 220 230 240
LRLRKVAHSD KPGSTSAVSF RDNVTSPLPS LLVVIAAIFI GFFLGKFIL
Length:249
Mass (Da):27,841
Last modified:January 9, 2007 - v3
Checksum:iA30D08E31F3379C1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti126L → P in AAD13579 (PubMed:9920726).Curated1
Sequence conflicti249L → S in AAD13579 (PubMed:9920726).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061875 mRNA Translation: AAH61875.2
AF086630 mRNA Translation: AAD13579.1
RefSeqiNP_113819.3, NM_031631.2
UniGeneiRn.162275

Genome annotation databases

EnsembliENSRNOT00000020681; ENSRNOP00000020681; ENSRNOG00000014765
GeneIDi58857
KEGGirno:58857
UCSCiRGD:61803 rat

Similar proteinsi

Entry informationi

Entry nameiVAPA_RAT
AccessioniPrimary (citable) accession number: Q9Z270
Secondary accession number(s): Q6P723
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: January 9, 2007
Last modified: March 28, 2018
This is version 137 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health