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Protein

Vesicle-associated membrane protein-associated protein B

Gene

Vapb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUnfolded protein response

Enzyme and pathway databases

ReactomeiR-RNO-1660661. Sphingolipid de novo biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein-associated protein B
Short name:
VAMP-B
Short name:
VAMP-associated protein B
Short name:
VAP-B
Gene namesi
Name:Vapb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi68326. Vapb.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 218CytoplasmicSequence analysisAdd BLAST217
Transmembranei219 – 239Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002134752 – 243Vesicle-associated membrane protein-associated protein BAdd BLAST242

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei146PhosphoserineBy similarity1
Cross-linki147Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei159PhosphoserineCombined sources1
Modified residuei206PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9Z269.
PRIDEiQ9Z269.

PTM databases

iPTMnetiQ9Z269.
PhosphoSitePlusiQ9Z269.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSRNOG00000005331.
GenevisibleiQ9Z269. RN.

Interactioni

Subunit structurei

Homodimer, and heterodimer with VAPA. Interacts with RMDN3, VAMP1 and VAMP2. Interacts (via MSP domain) with ZFYVE27. Interacts with KIF5A in a ZFYVE27-dependent manner. Interacts with STARD3 (via FFAT motif) (By similarity). Interacts with STARD3NL (via FFAT motif) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9Z269. 2 interactors.
MINTiMINT-4566897.
STRINGi10116.ENSRNOP00000007554.

Structurei

3D structure databases

ProteinModelPortaliQ9Z269.
SMRiQ9Z269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 124MSPPROSITE-ProRule annotationAdd BLAST118

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili161 – 196Sequence analysisAdd BLAST36

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0439. Eukaryota.
COG5066. LUCA.
GeneTreeiENSGT00390000006947.
HOGENOMiHOG000293182.
HOVERGENiHBG028551.
InParanoidiQ9Z269.
KOiK10707.
OMAiPHDIEIN.
OrthoDBiEOG091G0KJ2.
PhylomeDBiQ9Z269.
TreeFamiTF317024.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR013783. Ig-like_fold.
IPR000535. MSP_dom.
IPR008962. PapD-like.
IPR016763. VAP.
IPR030226. VapB.
PANTHERiPTHR10809. PTHR10809. 1 hit.
PTHR10809:SF86. PTHR10809:SF86. 1 hit.
PfamiView protein in Pfam
PF00635. Motile_Sperm. 1 hit.
PIRSFiPIRSF019693. VAMP-associated. 1 hit.
SUPFAMiSSF49354. SSF49354. 1 hit.
PROSITEiView protein in PROSITE
PS50202. MSP. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z269-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKVEQVLSL EPQHELKFRG PFTDVVTTNL KLGNPTDRNV CFKVKTTAPR
60 70 80 90 100
RYCVRPNSGV IDAGASLNVS VMLQPFDYDP NEKSKHKFMV QSMFAPPDTS
110 120 130 140 150
DMEAVWKEAK PEDLMDSKLR CVFELPAENA KPHDVEINKI MPTSASKTEA
160 170 180 190 200
PVAAKPLTSP LDDAEVKKVM EECRRLQGEV QRLREESRQL KEEDGLRARK
210 220 230 240
ALPSNSPMAA LAASGKEEGL SARLLALVVL FFIVGVIIGK IAL
Length:243
Mass (Da):26,916
Last modified:January 23, 2007 - v3
Checksum:i870B177B0798B4EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086631 mRNA. Translation: AAD13580.1.
BC065576 mRNA. Translation: AAH65576.1.
RefSeqiNP_068619.1. NM_021847.3.
UniGeneiRn.52500.

Genome annotation databases

EnsembliENSRNOT00000007554; ENSRNOP00000007554; ENSRNOG00000005331.
GeneIDi60431.
KEGGirno:60431.
UCSCiRGD:68326. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiVAPB_RAT
AccessioniPrimary (citable) accession number: Q9Z269
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: January 23, 2007
Last modified: July 5, 2017
This is version 122 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families