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Q9Z266

- SNAPN_MOUSE

UniProt

Q9Z266 - SNAPN_MOUSE

Protein

SNARE-associated protein Snapin

Gene

Snapin

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking and synaptic vesicle recycling. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release through its ability to potentiate the interaction of synaptotagmin with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells.3 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. SNARE binding Source: MGI

    GO - Biological processi

    1. anterograde axon cargo transport Source: UniProtKB
    2. anterograde synaptic vesicle transport Source: UniProtKB
    3. autophagic vacuole maturation Source: MGI
    4. calcium ion-dependent exocytosis Source: ParkinsonsUK-UCL
    5. intracellular protein transport Source: InterPro
    6. negative regulation of neuron projection development Source: ParkinsonsUK-UCL
    7. neuron projection development Source: UniProtKB
    8. synaptic transmission Source: MGI
    9. synaptic vesicle exocytosis Source: MGI
    10. synaptic vesicle fusion to presynaptic membrane Source: MGI
    11. synaptic vesicle maturation Source: MGI
    12. synaptic vesicle transport Source: UniProtKB
    13. terminal button organization Source: MGI

    Keywords - Biological processi

    Exocytosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    SNARE-associated protein Snapin
    Alternative name(s):
    Biogenesis of lysosome-related organelles complex 1 subunit 7
    Short name:
    BLOC-1 subunit 7
    Synaptosomal-associated protein 25-binding protein
    Short name:
    SNAP-associated protein
    Gene namesi
    Name:Snapin
    Synonyms:Bloc1s7, Snap25bp, Snapap
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:1333745. Snapin.

    Subcellular locationi

    Cytoplasm. Membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmperinuclear region. Golgi apparatus membrane. Cytoplasmic vesiclesecretory vesicle By similarity
    Note: Colocalizes with NANOS1 and PUM2 in the perinuclear region of germ cells By similarity. According to PubMed:12877659 it may be cytoplasmic and peripheral membrane bound. According to PubMed:11283605 it is anchored to the vesicular membrane through an N-terminal signal anchor.By similarity

    GO - Cellular componenti

    1. BLOC-1 complex Source: MGI
    2. cell junction Source: UniProtKB-KW
    3. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    4. cytosol Source: MGI
    5. Golgi membrane Source: UniProtKB-SubCell
    6. nucleolus Source: Ensembl
    7. perinuclear region of cytoplasm Source: UniProtKB
    8. secretory granule Source: UniProtKB
    9. synapse Source: MGI
    10. synaptic vesicle membrane Source: UniProtKB-SubCell
    11. transport vesicle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi42 – 421S → A: No effect. 1 Publication
    Mutagenesisi50 – 501S → A: Inhibition of phosphorylation. 1 Publication
    Mutagenesisi50 – 501S → D: 3.5-fold increase in SNAP25 binding. 1 Publication
    Mutagenesisi63 – 631T → A: No effect. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 136135SNARE-associated protein SnapinPRO_0000097557Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei10 – 101PhosphoserineBy similarity
    Modified residuei14 – 141PhosphothreonineBy similarity
    Modified residuei50 – 501Phosphoserine; by PKA; in vitro2 Publications
    Modified residuei129 – 1291PhosphotyrosineBy similarity
    Modified residuei133 – 1331PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by PKD, phosphorylation controls SNAPIN protein stability By similarity. Phosphorylated by CSNK1D/CK1.By similarity2 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9Z266.
    PaxDbiQ9Z266.
    PRIDEiQ9Z266.

    PTM databases

    PhosphoSiteiQ9Z266.

    Expressioni

    Tissue specificityi

    Strongly expressed in heart, brain, testis, kidney and liver; low expression in spleen, lung and skeletal muscle. In the testis, expressed in the seminiferous tubules.2 Publications

    Gene expression databases

    BgeeiQ9Z266.
    CleanExiMM_SNAPIN.
    GenevestigatoriQ9Z266.

    Interactioni

    Subunit structurei

    Interacts with CSNK1D, SNAP23 and STX4A but not with STX1A, VAMP2 and SYT1. Interacts with SNAP25; the interaction with SNAP25 is increased by its phosphorylation. Component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1) composed of BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. Octamer composed of one copy each BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. The BLOC-1 complex associates with the AP-3 protein complex and membrane protein cargos. Associates with the SNARE complex. Interacts with CNTRL, NANOS1, PUM2 and RGS7. Interacts with TOR1A; the interaction is direct and associates SNAPIN with the CSN complex.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Csnk1dQ06486-24EBI-6170320,EBI-7088890From a different organism.

    Protein-protein interaction databases

    BioGridi203363. 1 interaction.
    IntActiQ9Z266. 5 interactions.
    MINTiMINT-3389684.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni83 – 13654Interaction with TOR1ABy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili37 – 12690Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SNAPIN family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG83669.
    GeneTreeiENSGT00390000008274.
    HOGENOMiHOG000253926.
    HOVERGENiHBG056744.
    InParanoidiQ9Z266.
    OMAiHVHSVRE.
    OrthoDBiEOG7SXW5D.
    PhylomeDBiQ9Z266.
    TreeFamiTF319577.

    Family and domain databases

    InterProiIPR017246. Snapin.
    IPR028119. Snapin/Pallidin/Snn1.
    [Graphical view]
    PANTHERiPTHR31305. PTHR31305. 1 hit.
    PfamiPF14712. Snapin_Pallidin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037631. Snapin. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9Z266-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAAGSAAVS GAGTPVAGPT GRDLFAEGLL EFLRPAVQQL DSHVHAVRES    50
    QVELREQIDN LATELCRINE DQKVALDLDP YVKKLLNARR RVVLVNNILQ 100
    NAQERLRRLN HSVAKETARR RAMLDSGVYP PGSPSK 136
    Length:136
    Mass (Da):14,904
    Last modified:May 1, 1999 - v1
    Checksum:iC81AC2ABCDCA21FA
    GO
    Isoform 2 (identifier: Q9Z266-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         2-54: Missing.
         55-63: REQIDNLAT → MLVAHFLFP

    Show »
    Length:83
    Mass (Da):9,582
    Checksum:i0E42F823DD655B55
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei2 – 5453Missing in isoform 2. 1 PublicationVSP_009165Add
    BLAST
    Alternative sequencei55 – 639REQIDNLAT → MLVAHFLFP in isoform 2. 1 PublicationVSP_009166

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF086838 mRNA. Translation: AAD11418.1.
    AK007458 mRNA. Translation: BAB25049.1.
    AK048007 mRNA. Translation: BAC33212.1.
    AK050151 mRNA. Translation: BAC34095.1.
    AK077840 mRNA. Translation: BAC37029.1.
    AK080718 mRNA. Translation: BAC37991.1.
    AK152057 mRNA. Translation: BAE30913.1.
    BC006744 mRNA. Translation: AAH06744.1.
    BC048691 mRNA. Translation: AAH48691.1.
    CCDSiCCDS17531.1. [Q9Z266-1]
    RefSeqiNP_598615.1. NM_133854.3. [Q9Z266-1]
    UniGeneiMm.331182.

    Genome annotation databases

    EnsembliENSMUST00000149884; ENSMUSP00000122090; ENSMUSG00000001018. [Q9Z266-1]
    GeneIDi20615.
    KEGGimmu:20615.
    UCSCiuc008qck.2. mouse. [Q9Z266-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF086838 mRNA. Translation: AAD11418.1 .
    AK007458 mRNA. Translation: BAB25049.1 .
    AK048007 mRNA. Translation: BAC33212.1 .
    AK050151 mRNA. Translation: BAC34095.1 .
    AK077840 mRNA. Translation: BAC37029.1 .
    AK080718 mRNA. Translation: BAC37991.1 .
    AK152057 mRNA. Translation: BAE30913.1 .
    BC006744 mRNA. Translation: AAH06744.1 .
    BC048691 mRNA. Translation: AAH48691.1 .
    CCDSi CCDS17531.1. [Q9Z266-1 ]
    RefSeqi NP_598615.1. NM_133854.3. [Q9Z266-1 ]
    UniGenei Mm.331182.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203363. 1 interaction.
    IntActi Q9Z266. 5 interactions.
    MINTi MINT-3389684.

    PTM databases

    PhosphoSitei Q9Z266.

    Proteomic databases

    MaxQBi Q9Z266.
    PaxDbi Q9Z266.
    PRIDEi Q9Z266.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000149884 ; ENSMUSP00000122090 ; ENSMUSG00000001018 . [Q9Z266-1 ]
    GeneIDi 20615.
    KEGGi mmu:20615.
    UCSCi uc008qck.2. mouse. [Q9Z266-1 ]

    Organism-specific databases

    CTDi 23557.
    MGIi MGI:1333745. Snapin.

    Phylogenomic databases

    eggNOGi NOG83669.
    GeneTreei ENSGT00390000008274.
    HOGENOMi HOG000253926.
    HOVERGENi HBG056744.
    InParanoidi Q9Z266.
    OMAi HVHSVRE.
    OrthoDBi EOG7SXW5D.
    PhylomeDBi Q9Z266.
    TreeFami TF319577.

    Miscellaneous databases

    NextBioi 298987.
    PROi Q9Z266.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9Z266.
    CleanExi MM_SNAPIN.
    Genevestigatori Q9Z266.

    Family and domain databases

    InterProi IPR017246. Snapin.
    IPR028119. Snapin/Pallidin/Snn1.
    [Graphical view ]
    PANTHERi PTHR31305. PTHR31305. 1 hit.
    Pfami PF14712. Snapin_Pallidin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037631. Snapin. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Snapin: a SNARE-associated protein implicated in synaptic transmission."
      Ilardi J.M., Mochida S., Sheng Z.-H.
      Nat. Neurosci. 2:119-124(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH SNAP25.
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Bone marrow, Forelimb, Head, Liver, Pancreas and Retina.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Limb and Mammary tumor.
    4. "Identification and characterization of Snapin as a ubiquitously expressed SNARE-binding protein that interacts with SNAP23 in non-neuronal cells."
      Buxton P., Zhang X.-M., Walsh B., Sriratana A., Schenberg I., Manickam E., Rowe T.
      Biochem. J. 375:433-440(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH SNAP23 AND STX4A.
    5. "Phosphorylation of Snapin by PKA modulates its interaction with the SNARE complex."
      Chheda M.G., Ashery U., Thakur P., Rettig J., Sheng Z.-H.
      Nat. Cell Biol. 3:331-338(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-50, INTERACTION WITH SNAP25, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-42; SER-50 AND THR-63.
    6. "Regulation of the exocytotic machinery by cAMP-dependent protein kinase: implications for presynaptic plasticity."
      Evans G.J., Morgan A.
      Biochem. Soc. Trans. 31:824-827(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON PHOSPHORYLATION.
    7. "Casein kinase 1 delta (CK1delta) interacts with the SNARE associated protein snapin."
      Wolff S., Stoeter M., Giamas G., Piesche M., Henne-Bruns D., Banting G., Knippschild U.
      FEBS Lett. 580:6477-6484(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION BY CSNK1D/CK1, SUBCELLULAR LOCATION, INTERACTION WITH CSNK1D.
    8. Cited for: FUNCTION.
    9. "The SNARE-associated component SNAPIN binds PUMILIO2 and NANOS1 proteins in human male germ cells."
      Ginter-Matuszewska B., Spik A., Rembiszewska A., Koyias C., Kupryjanczyk J., Jaruzelska J.
      Mol. Hum. Reprod. 15:173-179(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    10. "The dysbindin-containing complex (BLOC-1) in brain: developmental regulation, interaction with SNARE proteins and role in neurite outgrowth."
      Ghiani C.A., Starcevic M., Rodriguez-Fernandez I.A., Nazarian R., Cheli V.T., Chan L.N., Malvar J.S., de Vellis J., Sabatti C., Dell'Angelica E.C.
      Mol. Psychiatry 15:204-215(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse."
      Larimore J., Tornieri K., Ryder P.V., Gokhale A., Zlatic S.A., Craige B., Lee J.D., Talbot K., Pare J.F., Smith Y., Faundez V.
      Mol. Biol. Cell 22:4854-4867(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ASSOCIATION WITH THE AP-3 COMPLEX.

    Entry informationi

    Entry nameiSNAPN_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z266
    Secondary accession number(s): Q3U8V4, Q922V7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 16, 2004
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3