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Protein

SNARE-associated protein Snapin

Gene

Snapin

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking and synaptic vesicle recycling. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release through its ability to potentiate the interaction of synaptotagmin with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells (PubMed:16760431, PubMed:19546860, PubMed:21998198). As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor (By similarity).By similarity3 Publications

GO - Molecular functioni

GO - Biological processi

  • anterograde axonal transport Source: UniProtKB
  • anterograde synaptic vesicle transport Source: UniProtKB
  • autophagosome maturation Source: MGI
  • calcium ion regulated exocytosis Source: ParkinsonsUK-UCL
  • cellular protein-containing complex localization Source: MGI
  • chemical synaptic transmission Source: MGI
  • endosome to lysosome transport Source: MGI
  • intracellular protein transport Source: InterPro
  • late endosome to lysosome transport Source: MGI
  • lysosomal lumen acidification Source: MGI
  • lysosome localization Source: UniProtKB
  • lysosome organization Source: MGI
  • negative regulation of neuron projection development Source: ParkinsonsUK-UCL
  • neuron projection development Source: UniProtKB
  • protein maturation Source: MGI
  • regulation of protein binding Source: MGI
  • retrograde axonal transport Source: MGI
  • synaptic vesicle exocytosis Source: MGI
  • synaptic vesicle fusion to presynaptic active zone membrane Source: MGI
  • synaptic vesicle maturation Source: MGI
  • synaptic vesicle transport Source: UniProtKB
  • terminal button organization Source: MGI

Keywordsi

Biological processExocytosis

Enzyme and pathway databases

ReactomeiR-MMU-432722 Golgi Associated Vesicle Biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
SNARE-associated protein Snapin
Alternative name(s):
Biogenesis of lysosome-related organelles complex 1 subunit 7
Short name:
BLOC-1 subunit 7
Synaptosomal-associated protein 25-binding protein
Short name:
SNAP-associated protein
Gene namesi
Name:Snapin
Synonyms:Bloc1s7, Snap25bp, Snapap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:1333745 Snapin

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Lysosome, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42S → A: No effect. 1 Publication1
Mutagenesisi50S → A: Inhibition of phosphorylation. 1 Publication1
Mutagenesisi50S → D: 3.5-fold increase in SNAP25 binding. 1 Publication1
Mutagenesisi63T → A: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000975572 – 136SNARE-associated protein SnapinAdd BLAST135

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei10PhosphoserineBy similarity1
Modified residuei14PhosphothreonineBy similarity1
Modified residuei50Phosphoserine; by PKA; in vitro1 Publication1
Modified residuei126PhosphoserineBy similarity1
Modified residuei129PhosphotyrosineBy similarity1
Modified residuei133PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by PKD, phosphorylation controls SNAPIN protein stability (By similarity). Phosphorylated by CSNK1D/CK1.By similarity2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Z266
PaxDbiQ9Z266
PeptideAtlasiQ9Z266
PRIDEiQ9Z266

PTM databases

iPTMnetiQ9Z266
PhosphoSitePlusiQ9Z266

Expressioni

Tissue specificityi

Strongly expressed in heart, brain, testis, kidney and liver; low expression in spleen, lung and skeletal muscle. In the testis, expressed in the seminiferous tubules.2 Publications

Gene expression databases

BgeeiENSMUSG00000001018
CleanExiMM_SNAPIN
ExpressionAtlasiQ9Z266 baseline and differential
GenevisibleiQ9Z266 MM

Interactioni

Subunit structurei

Component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1) composed of BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. Octamer composed of one copy each BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. The BLOC-1 complex associates with the AP-3 protein complex and membrane protein cargos (PubMed:21998198). Component of the BLOC-one-related complex (BORC) which is composed of BLOC1S1, BLOC1S2, BORCS5, BORCS6, BORCS7, BORCS8, KXD1 and SNAPIN (By similarity). Associates with the SNARE complex. Interacts with CSNK1D, SNAP23 and STX4A but not with STX1A, VAMP2 and SYT1 (PubMed:12877659, PubMed:17101137). Interacts with SNAP25; the interaction with SNAP25 is increased by its phosphorylation (PubMed:10195194, PubMed:11283605). Interacts with CNTRL, NANOS1, PUM2 and RGS7. Interacts with TOR1A; the interaction is direct and associates SNAPIN with the CSN complex (By similarity).By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Csnk1dQ06486-24EBI-6170320,EBI-7088890From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203363, 4 interactors
CORUMiQ9Z266
IntActiQ9Z266, 5 interactors
MINTiQ9Z266
STRINGi10090.ENSMUSP00000122090

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni83 – 136Interaction with TOR1ABy similarityAdd BLAST54

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili37 – 126Sequence analysisAdd BLAST90

Sequence similaritiesi

Belongs to the SNAPIN family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410J16C Eukaryota
ENOG4111JM9 LUCA
GeneTreeiENSGT00390000008274
HOGENOMiHOG000253926
HOVERGENiHBG056744
InParanoidiQ9Z266
KOiK20002
OMAiCRINEHQ
OrthoDBiEOG091G0WH1
PhylomeDBiQ9Z266
TreeFamiTF319577

Family and domain databases

InterProiView protein in InterPro
IPR017246 Snapin
IPR028119 Snapin/Pallidin/Snn1
PANTHERiPTHR31305 PTHR31305, 1 hit
PfamiView protein in Pfam
PF14712 Snapin_Pallidin, 1 hit
PIRSFiPIRSF037631 Snapin, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z266-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAGSAAVS GAGTPVAGPT GRDLFAEGLL EFLRPAVQQL DSHVHAVRES
60 70 80 90 100
QVELREQIDN LATELCRINE DQKVALDLDP YVKKLLNARR RVVLVNNILQ
110 120 130
NAQERLRRLN HSVAKETARR RAMLDSGVYP PGSPSK
Length:136
Mass (Da):14,904
Last modified:May 1, 1999 - v1
Checksum:iC81AC2ABCDCA21FA
GO
Isoform 2 (identifier: Q9Z266-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-54: Missing.
     55-63: REQIDNLAT → MLVAHFLFP

Show »
Length:82
Mass (Da):9,451
Checksum:i234286EC5C2B94D4
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0091651 – 54Missing in isoform 2. 1 PublicationAdd BLAST54
Alternative sequenceiVSP_00916655 – 63REQIDNLAT → MLVAHFLFP in isoform 2. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086838 mRNA Translation: AAD11418.1
AK007458 mRNA Translation: BAB25049.1
AK048007 mRNA Translation: BAC33212.1
AK050151 mRNA Translation: BAC34095.1
AK077840 mRNA Translation: BAC37029.1
AK080718 mRNA Translation: BAC37991.1
AK152057 mRNA Translation: BAE30913.1
BC006744 mRNA Translation: AAH06744.1
BC048691 mRNA Translation: AAH48691.1
CCDSiCCDS17531.1 [Q9Z266-1]
RefSeqiNP_598615.1, NM_133854.3 [Q9Z266-1]
UniGeneiMm.331182

Genome annotation databases

EnsembliENSMUST00000149884; ENSMUSP00000122090; ENSMUSG00000001018 [Q9Z266-1]
GeneIDi20615
KEGGimmu:20615
UCSCiuc008qck.2 mouse [Q9Z266-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSNAPN_MOUSE
AccessioniPrimary (citable) accession number: Q9Z266
Secondary accession number(s): Q3U8V4, Q922V7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: May 1, 1999
Last modified: April 25, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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