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Q9Z255

- UBE2A_MOUSE

UniProt

Q9Z255 - UBE2A_MOUSE

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Protein

Ubiquitin-conjugating enzyme E2 A

Gene

Ube2a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA (By similarity).By similarity

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. histone H2A ubiquitination Source: Ensembl
  3. in utero embryonic development Source: MGI
  4. maternal process involved in female pregnancy Source: MGI
  5. positive regulation of cell proliferation Source: Ensembl
  6. protein autoubiquitination Source: Ensembl
  7. protein K11-linked ubiquitination Source: UniProtKB
  8. protein K48-linked ubiquitination Source: UniProtKB
  9. response to UV Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 A (EC:6.3.2.19)
Alternative name(s):
RAD6 homolog A
Short name:
HR6A
Short name:
mHR6A
Ubiquitin carrier protein A
Ubiquitin-protein ligase A
Gene namesi
Name:Ube2a
Synonyms:Rad6a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:102959. Ube2a.

Subcellular locationi

GO - Cellular componenti

  1. chromatin Source: MGI
  2. HULC complex Source: UniProtKB
  3. nuclear chromatin Source: MGI
  4. XY body Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152Ubiquitin-conjugating enzyme E2 APRO_0000082446Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei120 – 1201Phosphoserine; by CDK9By similarity

Post-translational modificationi

Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z255.
PaxDbiQ9Z255.
PRIDEiQ9Z255.

PTM databases

PhosphoSiteiQ9Z255.

Expressioni

Gene expression databases

BgeeiQ9Z255.
ExpressionAtlasiQ9Z255. baseline and differential.
GenevestigatoriQ9Z255.

Interactioni

Subunit structurei

Interacts with RAD18 and WAC.

Protein-protein interaction databases

BioGridi204414. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9Z255.
SMRiQ9Z255. Positions 2-149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiQ9Z255.
KOiK10573.
OMAiPVPDNVM.
OrthoDBiEOG7M0NTH.
PhylomeDBiQ9Z255.
TreeFamiTF101128.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z255-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED
60 70 80 90 100
GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY
110 120 130 140 150
DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR

DC
Length:152
Mass (Da):17,315
Last modified:May 1, 1999 - v1
Checksum:i0AAEB5B7770E47E2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF089812 mRNA. Translation: AAC64563.1.
AF383148 mRNA. Translation: AAK62984.1.
BC026053 mRNA. Translation: AAH26053.1.
CCDSiCCDS30064.1.
RefSeqiNP_062642.1. NM_019668.3.
UniGeneiMm.395649.

Genome annotation databases

EnsembliENSMUST00000016452; ENSMUSP00000016452; ENSMUSG00000016308.
GeneIDi22209.
KEGGimmu:22209.
UCSCiuc009sxv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF089812 mRNA. Translation: AAC64563.1 .
AF383148 mRNA. Translation: AAK62984.1 .
BC026053 mRNA. Translation: AAH26053.1 .
CCDSi CCDS30064.1.
RefSeqi NP_062642.1. NM_019668.3.
UniGenei Mm.395649.

3D structure databases

ProteinModelPortali Q9Z255.
SMRi Q9Z255. Positions 2-149.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204414. 2 interactions.

PTM databases

PhosphoSitei Q9Z255.

Proteomic databases

MaxQBi Q9Z255.
PaxDbi Q9Z255.
PRIDEi Q9Z255.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000016452 ; ENSMUSP00000016452 ; ENSMUSG00000016308 .
GeneIDi 22209.
KEGGi mmu:22209.
UCSCi uc009sxv.1. mouse.

Organism-specific databases

CTDi 7319.
MGIi MGI:102959. Ube2a.

Phylogenomic databases

eggNOGi COG5078.
GeneTreei ENSGT00730000110565.
HOGENOMi HOG000233454.
HOVERGENi HBG063308.
InParanoidi Q9Z255.
KOi K10573.
OMAi PVPDNVM.
OrthoDBi EOG7M0NTH.
PhylomeDBi Q9Z255.
TreeFami TF101128.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

NextBioi 302201.
PROi Q9Z255.
SOURCEi Search...

Gene expression databases

Bgeei Q9Z255.
ExpressionAtlasi Q9Z255. baseline and differential.
Genevestigatori Q9Z255.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of mHR6A, a gene highly homologous to the male fertility gene mHR6B."
    Roest H.P., van Klaveren J., Koken M.H.M., Vermey M., van Cappellen W.A., Baarends W.M., Hoogerbrugge J.W., Bootsma D., Hoeijmakers J.H.J., Grootegoed J.A., de Wit J.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BCBA.
    Tissue: Brain.
  2. Kwon Y.T., Varshavsky A.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Skeletal muscle.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiUBE2A_MOUSE
AccessioniPrimary (citable) accession number: Q9Z255
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 4, 2003
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3