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Q9Z255

- UBE2A_MOUSE

UniProt

Q9Z255 - UBE2A_MOUSE

Protein

Ubiquitin-conjugating enzyme E2 A

Gene

Ube2a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA By similarity.By similarity

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: MGI
    4. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. DNA repair Source: UniProtKB-KW
    2. histone H2A ubiquitination Source: Ensembl
    3. in utero embryonic development Source: MGI
    4. maternal process involved in female pregnancy Source: MGI
    5. positive regulation of cell proliferation Source: Ensembl
    6. protein autoubiquitination Source: Ensembl
    7. protein K11-linked ubiquitination Source: UniProtKB
    8. protein K48-linked ubiquitination Source: UniProtKB
    9. response to UV Source: Ensembl

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    DNA damage, DNA repair, Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 A (EC:6.3.2.19)
    Alternative name(s):
    RAD6 homolog A
    Short name:
    HR6A
    Short name:
    mHR6A
    Ubiquitin carrier protein A
    Ubiquitin-protein ligase A
    Gene namesi
    Name:Ube2a
    Synonyms:Rad6a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:102959. Ube2a.

    Subcellular locationi

    GO - Cellular componenti

    1. chromatin Source: MGI
    2. HULC complex Source: UniProtKB
    3. nuclear chromatin Source: MGI
    4. XY body Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 152152Ubiquitin-conjugating enzyme E2 APRO_0000082446Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei120 – 1201Phosphoserine; by CDK9By similarity

    Post-translational modificationi

    Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9Z255.
    PRIDEiQ9Z255.

    PTM databases

    PhosphoSiteiQ9Z255.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9Z255.
    BgeeiQ9Z255.
    GenevestigatoriQ9Z255.

    Interactioni

    Subunit structurei

    Interacts with RAD18 and WAC.

    Protein-protein interaction databases

    BioGridi204414. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z255.
    SMRiQ9Z255. Positions 2-149.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00730000110565.
    HOGENOMiHOG000233454.
    HOVERGENiHBG063308.
    InParanoidiQ9Z255.
    KOiK10573.
    OMAiPVPDNVM.
    OrthoDBiEOG7M0NTH.
    PhylomeDBiQ9Z255.
    TreeFamiTF101128.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z255-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED    50
    GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY 100
    DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR 150
    DC 152
    Length:152
    Mass (Da):17,315
    Last modified:May 1, 1999 - v1
    Checksum:i0AAEB5B7770E47E2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF089812 mRNA. Translation: AAC64563.1.
    AF383148 mRNA. Translation: AAK62984.1.
    BC026053 mRNA. Translation: AAH26053.1.
    CCDSiCCDS30064.1.
    RefSeqiNP_062642.1. NM_019668.3.
    UniGeneiMm.395649.

    Genome annotation databases

    EnsembliENSMUST00000016452; ENSMUSP00000016452; ENSMUSG00000016308.
    GeneIDi22209.
    KEGGimmu:22209.
    UCSCiuc009sxv.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF089812 mRNA. Translation: AAC64563.1 .
    AF383148 mRNA. Translation: AAK62984.1 .
    BC026053 mRNA. Translation: AAH26053.1 .
    CCDSi CCDS30064.1.
    RefSeqi NP_062642.1. NM_019668.3.
    UniGenei Mm.395649.

    3D structure databases

    ProteinModelPortali Q9Z255.
    SMRi Q9Z255. Positions 2-149.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204414. 2 interactions.

    PTM databases

    PhosphoSitei Q9Z255.

    Proteomic databases

    PaxDbi Q9Z255.
    PRIDEi Q9Z255.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000016452 ; ENSMUSP00000016452 ; ENSMUSG00000016308 .
    GeneIDi 22209.
    KEGGi mmu:22209.
    UCSCi uc009sxv.1. mouse.

    Organism-specific databases

    CTDi 7319.
    MGIi MGI:102959. Ube2a.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00730000110565.
    HOGENOMi HOG000233454.
    HOVERGENi HBG063308.
    InParanoidi Q9Z255.
    KOi K10573.
    OMAi PVPDNVM.
    OrthoDBi EOG7M0NTH.
    PhylomeDBi Q9Z255.
    TreeFami TF101128.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.

    Miscellaneous databases

    NextBioi 302201.
    PROi Q9Z255.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Z255.
    Bgeei Q9Z255.
    Genevestigatori Q9Z255.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of mHR6A, a gene highly homologous to the male fertility gene mHR6B."
      Roest H.P., van Klaveren J., Koken M.H.M., Vermey M., van Cappellen W.A., Baarends W.M., Hoogerbrugge J.W., Bootsma D., Hoeijmakers J.H.J., Grootegoed J.A., de Wit J.
      Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BCBA.
      Tissue: Brain.
    2. Kwon Y.T., Varshavsky A.
      Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Skeletal muscle.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiUBE2A_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z255
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 4, 2003
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3