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Q9Z255 (UBE2A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 A

EC=6.3.2.19
Alternative name(s):
RAD6 homolog A
Short name=HR6A
Short name=mHR6A
Ubiquitin carrier protein A
Ubiquitin-protein ligase A
Gene names
Name:Ube2a
Synonyms:Rad6a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length152 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA By similarity.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with RAD18 and WAC.

Post-translational modification

Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B By similarity.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
Ubl conjugation pathway
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

histone H2A ubiquitination

Inferred from electronic annotation. Source: Ensembl

in utero embryonic development

Inferred from genetic interaction PubMed 15169909. Source: MGI

maternal process involved in female pregnancy

Inferred from mutant phenotype PubMed 15169909. Source: MGI

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

protein K11-linked ubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein K48-linked ubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein autoubiquitination

Inferred from electronic annotation. Source: Ensembl

response to UV

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentHULC complex

Inferred from sequence or structural similarity. Source: UniProtKB

XY body

Inferred from direct assay PubMed 15383616. Source: MGI

chromatin

Inferred from direct assay PubMed 15657431. Source: MGI

nuclear chromatin

Inferred from direct assay PubMed 15383616. Source: MGI

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-protein ligase activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 152152Ubiquitin-conjugating enzyme E2 A
PRO_0000082446

Sites

Active site881Glycyl thioester intermediate By similarity

Amino acid modifications

Modified residue1201Phosphoserine; by CDK9 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9Z255 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 0AAEB5B7770E47E2

FASTA15217,315
        10         20         30         40         50         60 
MSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT 

        70         80         90        100        110        120 
EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS 

       130        140        150 
PANSQAAQLY QENKREYEKR VSAIVEQSWR DC 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of mHR6A, a gene highly homologous to the male fertility gene mHR6B."
Roest H.P., van Klaveren J., Koken M.H.M., Vermey M., van Cappellen W.A., Baarends W.M., Hoogerbrugge J.W., Bootsma D., Hoeijmakers J.H.J., Grootegoed J.A., de Wit J.
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BCBA.
Tissue: Brain.
[2]Kwon Y.T., Varshavsky A.
Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Skeletal muscle.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF089812 mRNA. Translation: AAC64563.1.
AF383148 mRNA. Translation: AAK62984.1.
BC026053 mRNA. Translation: AAH26053.1.
RefSeqNP_062642.1. NM_019668.3.
UniGeneMm.395649.

3D structure databases

ProteinModelPortalQ9Z255.
SMRQ9Z255. Positions 2-149.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204414. 2 interactions.

PTM databases

PhosphoSiteQ9Z255.

Proteomic databases

PaxDbQ9Z255.
PRIDEQ9Z255.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000016452; ENSMUSP00000016452; ENSMUSG00000016308.
GeneID22209.
KEGGmmu:22209.
UCSCuc009sxv.1. mouse.

Organism-specific databases

CTD7319.
MGIMGI:102959. Ube2a.

Phylogenomic databases

eggNOGCOG5078.
GeneTreeENSGT00730000110565.
HOGENOMHOG000233454.
HOVERGENHBG063308.
InParanoidQ9Z255.
KOK10573.
OMAPVPDNVM.
OrthoDBEOG7M0NTH.
PhylomeDBQ9Z255.
TreeFamTF101128.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ9Z255.
BgeeQ9Z255.
GenevestigatorQ9Z255.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302201.
PROQ9Z255.
SOURCESearch...

Entry information

Entry nameUBE2A_MOUSE
AccessionPrimary (citable) accession number: Q9Z255
Entry history
Integrated into UniProtKB/Swiss-Prot: August 4, 2003
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot