Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin-conjugating enzyme E2 A

Gene

Ube2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA (By similarity).By similarity

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. small conjugating protein transferase activity Source: GO_Central
  4. ubiquitin conjugating enzyme activity Source: MGI
  5. ubiquitin protein ligase activity Source: GO_Central
  6. ubiquitin protein ligase binding Source: MGI
  7. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. DNA repair Source: MGI
  2. histone H2A ubiquitination Source: MGI
  3. histone ubiquitination Source: GO_Central
  4. in utero embryonic development Source: MGI
  5. maternal process involved in female pregnancy Source: MGI
  6. positive regulation of cell proliferation Source: MGI
  7. proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  8. protein autoubiquitination Source: MGI
  9. protein K11-linked ubiquitination Source: UniProtKB
  10. protein K48-linked ubiquitination Source: UniProtKB
  11. protein polyubiquitination Source: GO_Central
  12. response to UV Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 A (EC:6.3.2.19)
Alternative name(s):
RAD6 homolog A
Short name:
HR6A
Short name:
mHR6A
Ubiquitin carrier protein A
Ubiquitin-protein ligase A
Gene namesi
Name:Ube2a
Synonyms:Rad6a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:102959. Ube2a.

Subcellular locationi

GO - Cellular componenti

  1. chromatin Source: MGI
  2. cytoplasm Source: GO_Central
  3. HULC complex Source: UniProtKB
  4. nuclear chromatin Source: MGI
  5. XY body Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152Ubiquitin-conjugating enzyme E2 APRO_0000082446Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei120 – 1201Phosphoserine; by CDK9By similarity

Post-translational modificationi

Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z255.
PaxDbiQ9Z255.
PRIDEiQ9Z255.

PTM databases

PhosphoSiteiQ9Z255.

Expressioni

Gene expression databases

BgeeiQ9Z255.
ExpressionAtlasiQ9Z255. baseline and differential.
GenevestigatoriQ9Z255.

Interactioni

Subunit structurei

Interacts with RAD18 and WAC.

Protein-protein interaction databases

BioGridi204414. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9Z255.
SMRiQ9Z255. Positions 2-149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiQ9Z255.
KOiK10573.
OMAiPVPDNVM.
OrthoDBiEOG7M0NTH.
PhylomeDBiQ9Z255.
TreeFamiTF101128.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z255-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED
60 70 80 90 100
GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY
110 120 130 140 150
DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR

DC
Length:152
Mass (Da):17,315
Last modified:May 1, 1999 - v1
Checksum:i0AAEB5B7770E47E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF089812 mRNA. Translation: AAC64563.1.
AF383148 mRNA. Translation: AAK62984.1.
BC026053 mRNA. Translation: AAH26053.1.
CCDSiCCDS30064.1.
RefSeqiNP_062642.1. NM_019668.3.
UniGeneiMm.395649.

Genome annotation databases

EnsembliENSMUST00000016452; ENSMUSP00000016452; ENSMUSG00000016308.
GeneIDi22209.
KEGGimmu:22209.
UCSCiuc009sxv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF089812 mRNA. Translation: AAC64563.1.
AF383148 mRNA. Translation: AAK62984.1.
BC026053 mRNA. Translation: AAH26053.1.
CCDSiCCDS30064.1.
RefSeqiNP_062642.1. NM_019668.3.
UniGeneiMm.395649.

3D structure databases

ProteinModelPortaliQ9Z255.
SMRiQ9Z255. Positions 2-149.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204414. 2 interactions.

PTM databases

PhosphoSiteiQ9Z255.

Proteomic databases

MaxQBiQ9Z255.
PaxDbiQ9Z255.
PRIDEiQ9Z255.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000016452; ENSMUSP00000016452; ENSMUSG00000016308.
GeneIDi22209.
KEGGimmu:22209.
UCSCiuc009sxv.1. mouse.

Organism-specific databases

CTDi7319.
MGIiMGI:102959. Ube2a.

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiQ9Z255.
KOiK10573.
OMAiPVPDNVM.
OrthoDBiEOG7M0NTH.
PhylomeDBiQ9Z255.
TreeFamiTF101128.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiREACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

NextBioi302201.
PROiQ9Z255.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z255.
ExpressionAtlasiQ9Z255. baseline and differential.
GenevestigatoriQ9Z255.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of mHR6A, a gene highly homologous to the male fertility gene mHR6B."
    Roest H.P., van Klaveren J., Koken M.H.M., Vermey M., van Cappellen W.A., Baarends W.M., Hoogerbrugge J.W., Bootsma D., Hoeijmakers J.H.J., Grootegoed J.A., de Wit J.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BCBA.
    Tissue: Brain.
  2. Kwon Y.T., Varshavsky A.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Skeletal muscle.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiUBE2A_MOUSE
AccessioniPrimary (citable) accession number: Q9Z255
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 4, 2003
Last sequence update: May 1, 1999
Last modified: February 4, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.