Ubiquitin-conjugating enzyme E2 A - Mus musculus (Mouse)

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Q9Z255 (UBE2A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 A
EC=6.3.2.19

Alternative name(s):
RAD6 homolog A
Short name=HR6A
Short name=mHR6A
Ubiquitin carrier protein A
Ubiquitin-protein ligase A

Gene names

Name:	Ube2a
Synonyms:	Rad6a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with RAD18 and WAC.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW histone H2A ubiquitination Inferred from electronic annotation. Source: Compara in utero embryonic development Inferred from genetic interaction PubMed 15169909. Source: MGI maternal process involved in female pregnancy Inferred from mutant phenotype PubMed 15169909. Source: MGI positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara protein K11-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K48-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein autoubiquitination Inferred from electronic annotation. Source: Compara response to UV Inferred from electronic annotation. Source: Compara
Cellular_component	HULC complex Inferred from sequence or structural similarity. Source: UniProtKB XY body Inferred from direct assay PubMed 15383616. Source: MGI nuclear chromatin Inferred from direct assay PubMed 15383616. Source: MGI
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 152

152

Ubiquitin-conjugating enzyme E2 A

PRO_0000082446

Sites

Active site

Glycyl thioester intermediate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q9Z255 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 0AAEB5B7770E47E2
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FASTA

152

17,315

        10         20         30         40         50         60 
MSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT 

        70         80         90        100        110        120 
EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS 

       130        140        150 
PANSQAAQLY QENKREYEKR VSAIVEQSWR DC

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of mHR6A, a gene highly homologous to the male fertility gene mHR6B." Roest H.P., van Klaveren J., Koken M.H.M., Vermey M., van Cappellen W.A., Baarends W.M., Hoogerbrugge J.W., Bootsma D., Hoeijmakers J.H.J., Grootegoed J.A., de Wit J. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BCBA. Tissue: Brain.
[2]	Kwon Y.T., Varshavsky A. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Skeletal muscle.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF089812 mRNA. Translation: AAC64563.1. AF383148 mRNA. Translation: AAK62984.1. BC026053 mRNA. Translation: AAH26053.1.
IPI	IPI00130521.
RefSeq	NP_062642.1. NM_019668.3.
UniGene	Mm.395649.
3D structure databases
ProteinModelPortal	Q9Z255.
ModBase	Search...
PTM databases
PhosphoSite	Q9Z255.
Proteomic databases
PaxDb	Q9Z255.
PRIDE	Q9Z255.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000016452; ENSMUSP00000016452; ENSMUSG00000016308.
GeneID	22209.
KEGG	mmu:22209.
Organism-specific databases
CTD	7319.
MGI	MGI:102959. Ube2a.
Phylogenomic databases
eggNOG	COG5078.
GeneTree	ENSGT00680000099547.
HOGENOM	HOG000233454.
HOVERGEN	HBG063308.
InParanoid	Q9Z255.
KO	K10573.
OrthoDB	EOG4RJG2S.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q9Z255.
Bgee	Q9Z255.
Genevestigator	Q9Z255.
GermOnline	ENSMUSG00000016308. Mus musculus.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	302201.
SOURCE	Search...

Entry information

Entry name

UBE2A_MOUSE

Accession

Primary (citable) accession number: Q9Z255

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 4, 2003
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents