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Protein

Protein lin-7 homolog B

Gene

Lin7b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. MPP5 via its L27 domain. May increase the amplitude of ASIC3 acid-evoked currents by stabilizing the channel at the cell surface (By similarity).By similarity1 Publication

GO - Molecular functioni

  • L27 domain binding Source: GO_Central
  • PDZ domain binding Source: RGD

GO - Biological processi

Keywordsi

Biological processExocytosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-212676. Dopamine Neurotransmitter Release Cycle.
R-RNO-5666185. RHO GTPases Activate Rhotekin and Rhophilins.
R-RNO-6794361. Neurexins and neuroligins.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein lin-7 homolog B
Short name:
Lin-7B
Alternative name(s):
Mammalian lin-seven protein 2
Short name:
MALS-2
Vertebrate lin-7 homolog 2
Short name:
Veli-2
Gene namesi
Name:Lin7b
Synonyms:Mals2, Veli1a, Veli2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620730. Lin7b.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse, Synaptosome, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001896281 – 207Protein lin-7 homolog BAdd BLAST207

Proteomic databases

PaxDbiQ9Z252.
PRIDEiQ9Z252.

PTM databases

iPTMnetiQ9Z252.
PhosphoSitePlusiQ9Z252.

Expressioni

Tissue specificityi

Expressed only in brain.3 Publications

Inductioni

Up-regulated by cell depolarization and calcium entry through L-type calcium channels.1 Publication

Gene expression databases

BgeeiENSRNOG00000020746.
GenevisibleiQ9Z252. RN.

Interactioni

Subunit structurei

Interacts with ASIC3 Forms two exclusive ternary complexes with CASK and APBA1 or CASKIN1. Can also interact with other modular proteins containing protein-protein interaction domains like MPP5, MPP6, MPP7, DLG1, DLG2 and DLG3 through its L27 domain. Interacts with DLG4 and GRIN2B as well as CDH1 and CTNNB1, the channels KCNJ12/Kir2.2, KCNJ4/Kir2.3 and probably KCNJ2/Kir2.1 and SLC6A12/BGT-1 via its PDZ domain. The association of LIN7A with cadherin and beta-catenin is calcium-dependent, occurs at synaptic junctions and requires the actin cytoskeleton. Interacts with EGFR, ERBB2, ERBB3 and ERBB4 with both PDZ and KID domains. Associates with KIF17 via APBA1. Interacts with RTKN (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • L27 domain binding Source: GO_Central
  • PDZ domain binding Source: RGD

Protein-protein interaction databases

BioGridi248805. 2 interactors.
CORUMiQ9Z252.
IntActiQ9Z252. 5 interactors.
MINTiMINT-1588548.
STRINGi10116.ENSRNOP00000028164.

Structurei

Secondary structure

1207
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 27Combined sources21
Helixi33 – 43Combined sources11
Helixi45 – 59Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UITX-ray2.05A/B/C/D3-66[»]
ProteinModelPortaliQ9Z252.
SMRiQ9Z252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 65L27PROSITE-ProRule annotationAdd BLAST56
Domaini93 – 175PDZPROSITE-ProRule annotationAdd BLAST83

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1 – 13Kinase interacting siteBy similarityAdd BLAST13

Domaini

The kinase interacting site is required for proper delivery of ERBB2 to the basolateral membrane.By similarity
The PDZ domain regulates endocytosis and recycling of the receptor at the membrane.By similarity
The L27 domain mediates interaction with CASK and is involved in the formation of multimeric complexes and the association of LIN7 to membranes.By similarity

Sequence similaritiesi

Belongs to the lin-7 family.Curated

Phylogenomic databases

eggNOGiKOG3550. Eukaryota.
ENOG410XP5T. LUCA.
GeneTreeiENSGT00550000074582.
HOGENOMiHOG000285929.
HOVERGENiHBG052329.
InParanoidiQ9Z252.
KOiK19931.
OMAiVLQSKFC.
OrthoDBiEOG091G0PR4.
PhylomeDBiQ9Z252.
TreeFamiTF316850.

Family and domain databases

Gene3Di1.10.287.650. 1 hit.
InterProiView protein in InterPro
IPR014775. L27_C.
IPR004172. L27_dom.
IPR017365. LIN7.
IPR001478. PDZ.
PfamiView protein in Pfam
PF02828. L27. 1 hit.
PF00595. PDZ. 1 hit.
PIRSFiPIRSF038039. Lin-7_homologue. 1 hit.
SMARTiView protein in SMART
SM00569. L27. 1 hit.
SM00228. PDZ. 1 hit.
SUPFAMiSSF101288. SSF101288. 1 hit.
SSF50156. SSF50156. 1 hit.
PROSITEiView protein in PROSITE
PS51022. L27. 1 hit.
PS50106. PDZ. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Z252-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALVEPLGL ERDVSRAVEL LERLQRSGEL PPQKLQALQR VLQSRFCSAI
60 70 80 90 100
REVYEQLYDT LDITGSAEVR AHATAKATVA AFTASEGHAH PRVVELPKTD
110 120 130 140 150
EGLGFNIMGG KEQNSPIYIS RVIPGGVADR HGGLKRGDQL LSVNGVSVEG
160 170 180 190 200
EHHEKAVELL KAAQGSVKLV VRYTPRVLEE MEARFEKMRS ARRRQQHHSY

SSLESRG
Length:207
Mass (Da):22,900
Last modified:May 1, 1999 - v1
Checksum:iBC6B6754B8C89B13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090133 mRNA. Translation: AAC78072.1.
RefSeqiNP_068526.1. NM_021758.1.
UniGeneiRn.44184.

Genome annotation databases

EnsembliENSRNOT00000028164; ENSRNOP00000028164; ENSRNOG00000020746.
GeneIDi60377.
KEGGirno:60377.

Similar proteinsi

Entry informationi

Entry nameiLIN7B_RAT
AccessioniPrimary (citable) accession number: Q9Z252
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: May 1, 1999
Last modified: September 27, 2017
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families