Reviewed, UniProtKB/Swiss-Prot Q9Z244 (GMPR1_RAT)

Names and origin

Protein names

Recommended name:
    GMP reductase 1
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase 1
      Short name=Guanosine monophosphate reductase 1

Gene names

Name:	Gmpr
Synonyms:	Gmpr1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides.
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the IMPDH/GMPR family.

Ontologies

Keywords
Ligand	Metal-binding NADP Potassium
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to cold Ref.1 Inferred from expression pattern. Source: RGD
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: EC potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	GMP reductase 1		PRO_0000093725
Regions
	Nucleotide binding	108 – 131	24	NADP By similarity
Sites
	Active site	186	1	Thioimidate intermediate By similarity
	Metal binding	181	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	183	1	Potassium; via carbonyl oxygen By similarity
	Binding site	219	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z244-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 8B35AE591E32B3EE	FASTA	345	37,488
	10 20 30 40 50 60 MPRIDADLKL DFKDVLLRPK RSSLKSRSEV DLERTFTFRN SKQTYSGIPV IVANMDTVGT 70 80 90 100 110 120 FEMAVVMSQH AMFTAIHKHY SLDDWKHFAE NHPECLQHVA VSSGSGQNDL EKMSLILEAV 130 140 150 160 170 180 PQVKFICLDV ANGYSEHFVE FVKLVRSKFP EHTIMAGNVV TGEMVEELIL SGADIIKVGV 190 200 210 220 230 240 GPGSVCTTRT KTGVGYPQLS AVIECADSAH GLKGHIISDG SCTCPGDVAK AFGAGADFVM 250 260 270 280 290 300 LGGMFSGHTE CAGEVIERNG QKLKLFYGMS SDTAMKKHAG GVAEYRASEG KTVEVPYKGD 310 320 330 340 VENTILDILG GLRSTCTYVG AAKLKELSRR ATFIRVTQQH NTVFG

References

[1]

"The guanosine monophosphate reductase gene is conserved in rats and its expression increases rapidly in brown adipose tissue during cold exposure." Salvatore D., Bartha T., Larsen P.R. J. Biol. Chem. 273:31092-31096(1998) [PubMed: 9813009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brown adipose tissue.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF090867 mRNA. Translation: AAC78657.1.
IPI	IPI00209232.
RefSeq	NP_476536.1.
UniGene	Rn.3862
3D structure databases
HSSP	HSSP built from PDB template 1ZFJ based on UniProtKB P50099.
SMR	Q9Z244. Positions 1-343.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000017250. Rattus norvegicus. [Contig view]
GeneID	117533.
KEGG	rno:117533.
Organism-specific databases
RGD	70980. Gmpr.
Phylogenomic databases
HOVERGEN	Q9Z244.
Enzyme and pathway databases
BRENDA	1.7.1.7. 248.
Gene expression databases
ArrayExpress	Q9Z244.
GermOnline	ENSRNOG00000017250. Rattus norvegicus.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR005993. GMP_reduct1. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF000235. GMP_reductase. 1 hit.
TIGRFAMs	TIGR01305. GMP_reduct_1. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	620325.

Entry information

Entry name

GMPR1_RAT

Accession

Primary (citable) accession number: Q9Z244

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)