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Protein

Phospholemman

Gene

Fxyd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which transports Na+ out of the cell and K+ into the cell (PubMed:15563542, PubMed:18065526). Inhibits NKA activity in its unphosphorylated state and stimulates activity when phosphorylated (By similarity). Reduces glutathionylation of the NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated inhibition of ATP1B1 (PubMed:21454534). Contributes to female sexual development by maintaining the excitability of neurons which secrete gonadotropin-releasing hormone (PubMed:19187398).By similarity2 Publications

GO - Molecular functioni

  • ion channel activity Source: InterPro
  • ion channel binding Source: BHF-UCL
  • sodium channel regulator activity Source: BHF-UCL

GO - Biological processi

  • negative regulation of protein glutathionylation Source: UniProtKB
  • positive regulation of sodium ion export from cell Source: BHF-UCL
  • potassium ion transport Source: UniProtKB-KW
  • regulation of cardiac muscle cell membrane potential Source: BHF-UCL
  • regulation of sodium ion transmembrane transporter activity Source: BHF-UCL
  • sodium ion transport Source: UniProtKB-KW

Keywordsi

Biological processIon transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport
LigandPotassium, Sodium

Enzyme and pathway databases

ReactomeiR-MMU-5578775. Ion homeostasis.
R-MMU-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
PhospholemmanBy similarity
Alternative name(s):
FXYD domain-containing ion transport regulator 1Imported
Sodium/potassium-transporting ATPase subunit FXYD1Curated
Gene namesi
Name:Fxyd1Imported
Synonyms:Plm1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1889273. Fxyd1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 35ExtracellularSequence analysisAdd BLAST15
Transmembranei36 – 56HelicalSequence analysisAdd BLAST21
Topological domaini57 – 92CytoplasmicSequence analysisAdd BLAST36

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Adults exhibit increased cardiac mass, larger cardiomyocytes, higher ejection fractions (the amount of blood pumped out of the ventricles with each contraction) and reduced sodium/potassium-transporting ATPase (NKA) activity in a mixed strain background of C57BL/6 and 129/SvJ (PubMed:15563542). Decreased cardiac contractile function and increased NKA activity in a congenic C57BL/6 background (PubMed:18065526). Increased glutathionylation of the NKA beta subunit ATP1B1 (PubMed:21454534). Decreased excitability of neurons which secrete gonadotropin-releasing hormone and delayed female puberty (PubMed:19187398).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20By similarityAdd BLAST20
ChainiPRO_000001036021 – 92PhospholemmanAdd BLAST72

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi60S-palmitoyl cysteineBy similarity1
Modified residuei62S-glutathionyl cysteineBy similarity1
Lipidationi62S-palmitoyl cysteineBy similarity1
Modified residuei79PhosphothreonineCombined sources1
Modified residuei82PhosphoserineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Modified residuei88Phosphoserine; by PKABy similarity1

Post-translational modificationi

Major plasma membrane substrate for cAMP-dependent protein kinase (PKA) and protein kinase C (PKC) in several different tissues. Phosphorylated in response to insulin and adrenergic stimulation. Phosphorylation at Ser-88 stimulates sodium/potassium-transporting ATPase activity while the unphosphorylated form inhibits sodium/potassium-transporting ATPase activity. Phosphorylation increases tetramerization, decreases binding to ATP1A1 and reduces inhibition of ATP1A1 activity. Phosphorylation at Ser-83 leads to greatly reduced interaction with ATP1A1, ATP1A2 and ATP1A3. May be phosphorylated by DMPK.By similarity
Palmitoylation increases half-life and stability and is enhanced upon phosphorylation at Ser-88 by PKA.By similarity

Keywords - PTMi

Glutathionylation, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiQ9Z239.
PaxDbiQ9Z239.
PeptideAtlasiQ9Z239.
PRIDEiQ9Z239.

PTM databases

iPTMnetiQ9Z239.
PhosphoSitePlusiQ9Z239.

Expressioni

Gene expression databases

BgeeiENSMUSG00000036570.
CleanExiMM_FXYD1.
ExpressionAtlasiQ9Z239. baseline and differential.
GenevisibleiQ9Z239. MM.

Interactioni

Subunit structurei

Homotetramer (By similarity). Monomer (By similarity). Regulatory subunit of the sodium/potassium-transporting ATPase (NKA) which is composed of a catalytic alpha subunit, a non-catalytic beta subunit and an additional regulatory subunit (By similarity). The monomeric form associates with NKA while the oligomeric form does not (PubMed:23532852). Interacts with the catalytic alpha-1 subunit ATP1A1 (PubMed:17283221). Also interacts with the catalytic alpha-2 and alpha-3 subunits ATP1A2 and ATP1A3 (By similarity). Very little interaction with the catalytic alpha subunits ATP1A1, ATP1A2 or ATP1A3 when phosphorylated at Ser-83 (By similarity). Interacts with the non-catalytic beta-1 subunit ATP1B1 (By similarity). Oxidative stress decreases interaction with ATP1A1 but increases interaction with ATP1B1 (By similarity).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048460.

Structurei

3D structure databases

ProteinModelPortaliQ9Z239.
SMRiQ9Z239.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The cytoplasmic domain is sufficient to regulate sodium/potassium-transporting ATPase activity.By similarity

Sequence similaritiesi

Belongs to the FXYD family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J08K. Eukaryota.
ENOG410YYN6. LUCA.
GeneTreeiENSGT00530000063932.
HOGENOMiHOG000234467.
HOVERGENiHBG008212.
InParanoidiQ9Z239.
KOiK13360.
OMAiQEPDPFT.
OrthoDBiEOG091G17BP.
PhylomeDBiQ9Z239.
TreeFamiTF333443.

Family and domain databases

InterProiView protein in InterPro
IPR000272. Ion-transport_regulator_FXYD.
PfamiView protein in Pfam
PF02038. ATP1G1_PLM_MAT8. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD005989. PD005989. 1 hit.
PROSITEiView protein in PROSITE
PS01310. FXYD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z239-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPGHILAL CVCLLSMASA EAPQEPDPFT YDYHTLRIGG LTIAGILFIL
60 70 80 90
GILIILSKRC RCKFNQQQRT GEPDEEEGTF RSSIRRLSSR RR
Length:92
Mass (Da):10,323
Last modified:May 1, 1999 - v1
Checksum:i0BDB1DC83417F3AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091390 Genomic DNA. Translation: AAD11781.1.
AF089734 mRNA. Translation: AAD41683.1.
AK002585 mRNA. Translation: BAB22208.1.
BC024671 mRNA. Translation: AAH24671.1.
CCDSiCCDS21123.1.
RefSeqiNP_062376.2. NM_019503.4.
NP_443717.1. NM_052991.4.
NP_443718.1. NM_052992.3.
NP_919302.1. NM_194321.2.
UniGeneiMm.1491.

Genome annotation databases

EnsembliENSMUST00000039909; ENSMUSP00000048460; ENSMUSG00000036570.
ENSMUST00000071697; ENSMUSP00000071617; ENSMUSG00000036570.
ENSMUST00000108110; ENSMUSP00000103745; ENSMUSG00000036570.
ENSMUST00000205439; ENSMUSP00000146064; ENSMUSG00000036570.
ENSMUST00000205778; ENSMUSP00000145818; ENSMUSG00000036570.
ENSMUST00000206012; ENSMUSP00000146295; ENSMUSG00000036570.
ENSMUST00000206305; ENSMUSP00000145589; ENSMUSG00000036570.
ENSMUST00000206328; ENSMUSP00000145712; ENSMUSG00000036570.
ENSMUST00000206474; ENSMUSP00000145920; ENSMUSG00000036570.
ENSMUST00000206860; ENSMUSP00000146288; ENSMUSG00000036570.
GeneIDi56188.
KEGGimmu:56188.
UCSCiuc009ghu.2. mouse.

Entry informationi

Entry nameiPLM_MOUSE
AccessioniPrimary (citable) accession number: Q9Z239
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: May 1, 1999
Last modified: October 25, 2017
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families