Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phospholemman

Gene

Fxyd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which transports Na+ out of the cell and K+ into the cell (PubMed:15563542, PubMed:18065526). Inhibits NKA activity in its unphosphorylated state and stimulates activity when phosphorylated (By similarity). Reduces glutathionylation of the NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated inhibition of ATP1B1 (PubMed:21454534). Contributes to female sexual development by maintaining the excitability of neurons which secrete gonadotropin-releasing hormone (PubMed:19187398).By similarity2 Publications

GO - Molecular functioni

  • ATPase binding Source: MGI
  • ion channel activity Source: InterPro
  • ion channel binding Source: BHF-UCL
  • myosin binding Source: MGI
  • sodium channel regulator activity Source: BHF-UCL

GO - Biological processi

Keywordsi

Biological processIon transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport
LigandPotassium, Sodium

Enzyme and pathway databases

ReactomeiR-MMU-5578775 Ion homeostasis
R-MMU-936837 Ion transport by P-type ATPases

Names & Taxonomyi

Protein namesi
Recommended name:
PhospholemmanBy similarity
Alternative name(s):
FXYD domain-containing ion transport regulator 1Imported
Sodium/potassium-transporting ATPase subunit FXYD1Curated
Gene namesi
Name:Fxyd1Imported
Synonyms:Plm1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1889273 Fxyd1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 35ExtracellularSequence analysisAdd BLAST15
Transmembranei36 – 56HelicalSequence analysisAdd BLAST21
Topological domaini57 – 92CytoplasmicSequence analysisAdd BLAST36

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Adults exhibit increased cardiac mass, larger cardiomyocytes, higher ejection fractions (the amount of blood pumped out of the ventricles with each contraction) and reduced sodium/potassium-transporting ATPase (NKA) activity in a mixed strain background of C57BL/6 and 129/SvJ (PubMed:15563542). Decreased cardiac contractile function and increased NKA activity in a congenic C57BL/6 background (PubMed:18065526). Increased glutathionylation of the NKA beta subunit ATP1B1 (PubMed:21454534). Decreased excitability of neurons which secrete gonadotropin-releasing hormone and delayed female puberty (PubMed:19187398).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20By similarityAdd BLAST20
ChainiPRO_000001036021 – 92PhospholemmanAdd BLAST72

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi60S-palmitoyl cysteineBy similarity1
Modified residuei62S-glutathionyl cysteine; alternateBy similarity1
Lipidationi62S-palmitoyl cysteine; alternateBy similarity1
Modified residuei79PhosphothreonineCombined sources1
Modified residuei82PhosphoserineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Modified residuei88Phosphoserine; by PKABy similarity1

Post-translational modificationi

Major plasma membrane substrate for cAMP-dependent protein kinase (PKA) and protein kinase C (PKC) in several different tissues. Phosphorylated in response to insulin and adrenergic stimulation. Phosphorylation at Ser-88 stimulates sodium/potassium-transporting ATPase activity while the unphosphorylated form inhibits sodium/potassium-transporting ATPase activity. Phosphorylation increases tetramerization, decreases binding to ATP1A1 and reduces inhibition of ATP1A1 activity. Phosphorylation at Ser-83 leads to greatly reduced interaction with ATP1A1, ATP1A2 and ATP1A3. May be phosphorylated by DMPK.By similarity
Palmitoylation increases half-life and stability and is enhanced upon phosphorylation at Ser-88 by PKA.By similarity

Keywords - PTMi

Glutathionylation, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiQ9Z239
PaxDbiQ9Z239
PeptideAtlasiQ9Z239
PRIDEiQ9Z239

PTM databases

iPTMnetiQ9Z239
PhosphoSitePlusiQ9Z239

Expressioni

Gene expression databases

BgeeiENSMUSG00000036570
CleanExiMM_FXYD1
ExpressionAtlasiQ9Z239 baseline and differential
GenevisibleiQ9Z239 MM

Interactioni

Subunit structurei

Homotetramer (By similarity). Monomer (By similarity). Regulatory subunit of the sodium/potassium-transporting ATPase (NKA) which is composed of a catalytic alpha subunit, a non-catalytic beta subunit and an additional regulatory subunit (By similarity). The monomeric form associates with NKA while the oligomeric form does not (PubMed:23532852). Interacts with the catalytic alpha-1 subunit ATP1A1 (PubMed:17283221). Also interacts with the catalytic alpha-2 and alpha-3 subunits ATP1A2 and ATP1A3 (By similarity). Very little interaction with the catalytic alpha subunits ATP1A1, ATP1A2 or ATP1A3 when phosphorylated at Ser-83 (By similarity). Interacts with the non-catalytic beta-1 subunit ATP1B1 (By similarity). Oxidative stress decreases interaction with ATP1A1 but increases interaction with ATP1B1 (By similarity).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207828, 1 interactor
STRINGi10090.ENSMUSP00000048460

Structurei

3D structure databases

ProteinModelPortaliQ9Z239
SMRiQ9Z239
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The cytoplasmic domain is sufficient to regulate sodium/potassium-transporting ATPase activity.By similarity

Sequence similaritiesi

Belongs to the FXYD family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J08K Eukaryota
ENOG410YYN6 LUCA
GeneTreeiENSGT00530000063932
HOGENOMiHOG000234467
HOVERGENiHBG008212
InParanoidiQ9Z239
KOiK13360
OMAiQEPDPFT
OrthoDBiEOG091G17BP
PhylomeDBiQ9Z239
TreeFamiTF333443

Family and domain databases

InterProiView protein in InterPro
IPR000272 Ion-transport_regulator_FXYD
PfamiView protein in Pfam
PF02038 ATP1G1_PLM_MAT8, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD005989 PD005989, 1 hit
PROSITEiView protein in PROSITE
PS01310 FXYD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z239-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPGHILAL CVCLLSMASA EAPQEPDPFT YDYHTLRIGG LTIAGILFIL
60 70 80 90
GILIILSKRC RCKFNQQQRT GEPDEEEGTF RSSIRRLSSR RR
Length:92
Mass (Da):10,323
Last modified:May 1, 1999 - v1
Checksum:i0BDB1DC83417F3AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091390 Genomic DNA Translation: AAD11781.1
AF089734 mRNA Translation: AAD41683.1
AK002585 mRNA Translation: BAB22208.1
BC024671 mRNA Translation: AAH24671.1
CCDSiCCDS21123.1
RefSeqiNP_062376.2, NM_019503.4
NP_443717.1, NM_052991.4
NP_443718.1, NM_052992.3
NP_919302.1, NM_194321.2
UniGeneiMm.1491

Genome annotation databases

EnsembliENSMUST00000039909; ENSMUSP00000048460; ENSMUSG00000036570
ENSMUST00000071697; ENSMUSP00000071617; ENSMUSG00000036570
ENSMUST00000108110; ENSMUSP00000103745; ENSMUSG00000036570
ENSMUST00000205439; ENSMUSP00000146064; ENSMUSG00000036570
ENSMUST00000205778; ENSMUSP00000145818; ENSMUSG00000036570
ENSMUST00000206012; ENSMUSP00000146295; ENSMUSG00000036570
ENSMUST00000206305; ENSMUSP00000145589; ENSMUSG00000036570
ENSMUST00000206328; ENSMUSP00000145712; ENSMUSG00000036570
ENSMUST00000206474; ENSMUSP00000145920; ENSMUSG00000036570
ENSMUST00000206860; ENSMUSP00000146288; ENSMUSG00000036570
GeneIDi56188
KEGGimmu:56188
UCSCiuc009ghu.2 mouse

Entry informationi

Entry nameiPLM_MOUSE
AccessioniPrimary (citable) accession number: Q9Z239
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: May 1, 1999
Last modified: June 20, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health