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Protein

N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2

Gene

B3GNT2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Catalyzes the initiation and elongation of poly-N-acetyllactosamine chains (PubMed:9892646). Probably constitutes the main polylactosamine synthase (PubMed:17890318).2 Publications

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R.By similarity

Cofactori

Mn2+1 Publication

Pathway: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • axon guidance Source: MGI
  • poly-N-acetyllactosamine biosynthetic process Source: UniProtKB
  • protein glycosylation Source: MGI
  • sensory perception of smell Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese

Enzyme and pathway databases

ReactomeiREACT_278772. O-linked glycosylation of mucins.
REACT_346228. Keratan sulfate biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2 (EC:2.4.1.149By similarity)
Alternative name(s):
Beta-1,3-N-acetylglucosaminyltransferase 1
Short name:
BGnT-1
Short name:
Beta-1,3-Gn-T1
Short name:
Beta3Gn-T1
Beta-1,3-galactosyltransferase 7
Short name:
Beta-1,3-GalTase 7
Short name:
Beta3Gal-T7
Short name:
Beta3GalT7
Short name:
b3Gal-T7
Beta-3-Gx-T7
UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
Short name:
BGnT-2
Short name:
Beta-1,3-Gn-T2
Short name:
Beta-1,3-N-acetylglucosaminyltransferase 2
Short name:
Beta3Gn-T2
UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7
Gene namesi
Name:B3GNT2
Synonyms:B3gnt1, Beta3gnt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1889505. B3gnt2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini29 – 325297LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Strongly reduced number polylactosamine structures on N-glycans in immunological tissues. B-cells and T-cells proliferate show hyperproliferation (PubMed:17890318). Mice also display defective accessory olfactory bulb innervation (PubMed:23006775). Impaired sexual behaviour. While female mice are fertile, males show a reduced rate of reproduction. Defects cannot be attributed to any physical defect in their reproductive organs, suggesting that the phenotype observed may result from an impaired sexual response to female mating partners.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2PRO_0000219171Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi30 – 301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi173 – 1731N-linked (GlcNAc...)1 Publication
Glycosylationi219 – 2191N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9Z222.
PRIDEiQ9Z222.

PTM databases

PhosphoSiteiQ9Z222.

Expressioni

Tissue specificityi

Expressed in heart, brain, lung, kidney and testis and, to a lesser extent, in liver and skeletal muscle. No expression in spleen.1 Publication

Gene expression databases

BgeeiQ9Z222.
ExpressionAtlasiQ9Z222. baseline.
GenevisibleiQ9Z222. MM.

Interactioni

Subunit structurei

Interacts with B3GNT8; this interaction greatly increases B3GNT2 catalytic activity, independently of B3GNT8 enzymatic activity.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000053528.

Structurei

3D structure databases

ProteinModelPortaliQ9Z222.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Sequence Analysis

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG262849.
GeneTreeiENSGT00760000118879.
HOGENOMiHOG000232195.
HOVERGENiHBG050653.
InParanoidiQ9Z222.
KOiK00741.
OMAiIDIWSRL.
OrthoDBiEOG7BGHKT.
PhylomeDBiQ9Z222.
TreeFamiTF318639.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVGRRRVKL LGILMMANVF IYLIVEVSKN SSQDKNGKGG VIIPKEKFWK
60 70 80 90 100
PPSTPRAYWN REQEKLNRWY NPILNRVANQ TGELATSPNT SHLSYCEPDS
110 120 130 140 150
TVMTAVTDFN NLPDRFKDFL LYLRCRNYSL LIDQPKKCAK KPFLLLAIKS
160 170 180 190 200
LIPHFARRQA IRESWGRETN VGNQTVVRVF LLGKTPPEDN HPDLSDMLKF
210 220 230 240 250
ESDKHQDILM WNYRDTFFNL SLKEVLFLRW VSTSCPDAEF VFKGDDDVFV
260 270 280 290 300
NTHHILNYLN SLSKSKAKDL FIGDVIHNAG PHRDKKLKYY IPEVFYTGVY
310 320 330 340 350
PPYAGGGGFL YSGPLALRLY SATSRVHLYP IDDVYTGMCL QKLGLVPEKH
360 370 380 390
KGFRTFDIEE KNKKNICSYI DLMLVHSRKP QEMIDIWSQL QSPNLKC
Length:397
Mass (Da):45,883
Last modified:October 25, 2004 - v3
Checksum:iD8BBEA1866C1D106
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti283 – 2831R → S in AAD09763 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF092050 mRNA. Translation: AAD09763.2.
AY043479 mRNA. Translation: AAK95359.1.
BC009075 mRNA. Translation: AAH09075.1.
CCDSiCCDS24471.1.
RefSeqiNP_001162585.1. NM_001169114.1.
NP_058584.3. NM_016888.5.
UniGeneiMm.395495.

Genome annotation databases

EnsembliENSMUST00000055549; ENSMUSP00000053528; ENSMUSG00000051650.
ENSMUST00000062844; ENSMUSP00000060247; ENSMUSG00000051650.
GeneIDi53625.
KEGGimmu:53625.
UCSCiuc007ieh.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

MGC32391: hypothetical protein MGC32391/hypothetical protein MGC32391

Functional Glycomics Gateway - GTase

beta 3 GlcNAc-T I

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF092050 mRNA. Translation: AAD09763.2.
AY043479 mRNA. Translation: AAK95359.1.
BC009075 mRNA. Translation: AAH09075.1.
CCDSiCCDS24471.1.
RefSeqiNP_001162585.1. NM_001169114.1.
NP_058584.3. NM_016888.5.
UniGeneiMm.395495.

3D structure databases

ProteinModelPortaliQ9Z222.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000053528.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

PTM databases

PhosphoSiteiQ9Z222.

Proteomic databases

MaxQBiQ9Z222.
PRIDEiQ9Z222.

Protocols and materials databases

DNASUi53625.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055549; ENSMUSP00000053528; ENSMUSG00000051650.
ENSMUST00000062844; ENSMUSP00000060247; ENSMUSG00000051650.
GeneIDi53625.
KEGGimmu:53625.
UCSCiuc007ieh.2. mouse.

Organism-specific databases

CTDi10678.
MGIiMGI:1889505. B3gnt2.

Phylogenomic databases

eggNOGiNOG262849.
GeneTreeiENSGT00760000118879.
HOGENOMiHOG000232195.
HOVERGENiHBG050653.
InParanoidiQ9Z222.
KOiK00741.
OMAiIDIWSRL.
OrthoDBiEOG7BGHKT.
PhylomeDBiQ9Z222.
TreeFamiTF318639.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_278772. O-linked glycosylation of mucins.
REACT_346228. Keratan sulfate biosynthesis.

Miscellaneous databases

ChiTaRSiB3gnt2. mouse.
NextBioi310369.
PROiQ9Z222.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z222.
ExpressionAtlasiQ9Z222. baseline.
GenevisibleiQ9Z222. MM.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A beta-1,3-N-acetylglucosaminyltransferase with poly-N-acetyllactosamine synthase activity is structurally related to beta-1,3-galactosyltransferases."
    Zhou D., Dinter A., Gutierrez Gallego R., Kamerling J.P., Vliegenthart J.F.G., Berger E.G., Hennet T.
    Proc. Natl. Acad. Sci. U.S.A. 96:406-411(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, COFACTOR, TISSUE SPECIFICITY.
    Strain: ICR1 Publication.
    Tissue: Neonatal brain1 Publication.
  2. Zhou D., Berger E.G., Hennet T.
    Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Molecular cloning and expression analysis of a mouse UDP-GlcNAc:Gal(beta1-4)Glc(NAc)-R beta1,3-N-acetylglucosaminyltransferase homologous to Drosophila melanogaster Brainiac and the beta1,3-galactosyltransferase family."
    Egan S., Cohen B., Sarkar M., Ying Y., Cohen S., Singh N., Wang W., Flock G., Goh T., Schachter H.
    Glycoconj. J. 17:867-875(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C.B17Imported.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NMRIImported.
    Tissue: Mammary glandImported.
  5. Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  6. "Activation of beta1,3-N-acetylglucosaminyltransferase-2 (beta3Gn-T2) by beta3Gn-T8. Possible involvement of beta3Gn-T8 in increasing poly-N-acetyllactosamine chains in differentiated HL-60 cells."
    Seko A., Yamashita K.
    J. Biol. Chem. 283:33094-33100(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH B3GNT8.
  7. "Impaired sexual behavior in male mice deficient for the beta1-3 N-acetylglucosaminyltransferase-I gene."
    Biellmann F., Henion T.R., Burki K., Hennet T.
    Mol. Reprod. Dev. 75:699-706(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  8. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-173.
  9. "beta3GnT2 null mice exhibit defective accessory olfactory bulb innervation."
    Henion T.R., Madany P.A., Faden A.A., Schwarting G.A.
    Mol. Cell. Neurosci. 52:73-86(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiB3GN2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z222
Secondary accession number(s): Q91V18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: October 25, 2004
Last modified: June 24, 2015
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

There is some conflicting nomenclature, as some groups still name this protein B3gnt1 (PubMed:18008318). The correct and official nomenclature is however B3gnt2.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.