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Q9Z222 (B3GN2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2

Short name=BGnT-2
Short name=Beta-1,3-Gn-T2
Short name=Beta-1,3-N-acetylglucosaminyltransferase 2
Short name=Beta3Gn-T2
EC=2.4.1.-
Alternative name(s):
Beta-1,3-N-acetylglucosaminyltransferase 1
Short name=BGnT-1
Short name=Beta-1,3-Gn-T1
Short name=Beta3Gn-T1
Beta-1,3-galactosyltransferase 7
Short name=Beta-1,3-GalTase 7
Short name=Beta3Gal-T7
Short name=Beta3GalT7
Short name=b3Gal-T7
Beta-3-Gx-T7
UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7
UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7
Gene names
Name:B3gnt2
Synonyms:B3gnt1, Beta3gnt
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the initiation and elongation of poly-N-acetyllactosamine chains. Ref.1

Cofactor

Manganese. Ref.1

Pathway

Protein modification; protein glycosylation.

Subunit structure

Interacts with B3GNT8; this interaction greatly increases B3GNT2 catalytic activity, independently of B3GNT8 enzymatic activity. Ref.5

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein Potential.

Tissue specificity

Expressed in heart, brain, lung, kidney and testis and, to a lesser extent, in liver and skeletal muscle. No expression in spleen. Ref.1

Sequence similarities

Belongs to the glycosyltransferase 31 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 397397UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
PRO_0000219171

Regions

Topological domain1 – 77Cytoplasmic Potential
Transmembrane8 – 2821Helical; Signal-anchor for type II membrane protein; Potential
Topological domain29 – 325297Lumenal Potential

Amino acid modifications

Glycosylation301N-linked (GlcNAc...) Potential
Glycosylation791N-linked (GlcNAc...) Potential
Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation1271N-linked (GlcNAc...) Potential
Glycosylation1731N-linked (GlcNAc...) Ref.6
Glycosylation2191N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict2831R → S in AAD09763. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9Z222 [UniParc].

Last modified October 25, 2004. Version 3.
Checksum: D8BBEA1866C1D106

FASTA39745,883
        10         20         30         40         50         60 
MSVGRRRVKL LGILMMANVF IYLIVEVSKN SSQDKNGKGG VIIPKEKFWK PPSTPRAYWN 

        70         80         90        100        110        120 
REQEKLNRWY NPILNRVANQ TGELATSPNT SHLSYCEPDS TVMTAVTDFN NLPDRFKDFL 

       130        140        150        160        170        180 
LYLRCRNYSL LIDQPKKCAK KPFLLLAIKS LIPHFARRQA IRESWGRETN VGNQTVVRVF 

       190        200        210        220        230        240 
LLGKTPPEDN HPDLSDMLKF ESDKHQDILM WNYRDTFFNL SLKEVLFLRW VSTSCPDAEF 

       250        260        270        280        290        300 
VFKGDDDVFV NTHHILNYLN SLSKSKAKDL FIGDVIHNAG PHRDKKLKYY IPEVFYTGVY 

       310        320        330        340        350        360 
PPYAGGGGFL YSGPLALRLY SATSRVHLYP IDDVYTGMCL QKLGLVPEKH KGFRTFDIEE 

       370        380        390 
KNKKNICSYI DLMLVHSRKP QEMIDIWSQL QSPNLKC 

« Hide

References

« Hide 'large scale' references
[1]"A beta-1,3-N-acetylglucosaminyltransferase with poly-N-acetyllactosamine synthase activity is structurally related to beta-1,3-galactosyltransferases."
Zhou D., Dinter A., Gutierrez Gallego R., Kamerling J.P., Vliegenthart J.F.G., Berger E.G., Hennet T.
Proc. Natl. Acad. Sci. U.S.A. 96:406-411(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, COFACTOR, TISSUE SPECIFICITY.
Strain: ICR.
Tissue: Neonatal brain.
[2]Zhou D., Berger E.G., Hennet T.
Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"Molecular cloning and expression analysis of a mouse UDP-GlcNAc:Gal(beta1-4)Glc(NAc)-R beta1,3-N-acetylglucosaminyltransferase homologous to Drosophila melanogaster Brainiac and the beta1,3-galactosyltransferase family."
Egan S., Cohen B., Sarkar M., Ying Y., Cohen S., Singh N., Wang W., Flock G., Goh T., Schachter H.
Glycoconj. J. 17:867-875(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C.B17.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NMRI.
Tissue: Mammary gland.
[5]"Activation of beta1,3-N-acetylglucosaminyltransferase-2 (beta3Gn-T2) by beta3Gn-T8. Possible involvement of beta3Gn-T8 in increasing poly-N-acetyllactosamine chains in differentiated HL-60 cells."
Seko A., Yamashita K.
J. Biol. Chem. 283:33094-33100(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH B3GNT8.
[6]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-173.
+Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

MGC32391: hypothetical protein MGC32391/hypothetical protein MGC32391

Functional Glycomics Gateway - GTase

beta 3 GlcNAc-T I

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF092050 mRNA. Translation: AAD09763.2.
AY043479 mRNA. Translation: AAK95359.1.
BC009075 mRNA. Translation: AAH09075.1.
CCDSCCDS24471.1.
RefSeqNP_001162585.1. NM_001169114.1.
NP_058584.3. NM_016888.5.
UniGeneMm.395495.

3D structure databases

ProteinModelPortalQ9Z222.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000060247.

Protein family/group databases

CAZyGT31. Glycosyltransferase Family 31.

PTM databases

PhosphoSiteQ9Z222.

Proteomic databases

PRIDEQ9Z222.

Protocols and materials databases

DNASU53625.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000055549; ENSMUSP00000053528; ENSMUSG00000051650.
ENSMUST00000062844; ENSMUSP00000060247; ENSMUSG00000051650.
GeneID53625.
KEGGmmu:53625.
UCSCuc007ieh.2. mouse.

Organism-specific databases

CTD10678.
MGIMGI:1889505. B3gnt2.

Phylogenomic databases

eggNOGNOG262849.
GeneTreeENSGT00740000115391.
HOGENOMHOG000232195.
HOVERGENHBG050653.
InParanoidQ9Z222.
KOK00741.
OMARNNICSY.
OrthoDBEOG7BGHKT.
PhylomeDBQ9Z222.
TreeFamTF318639.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

BgeeQ9Z222.
GenevestigatorQ9Z222.

Family and domain databases

InterProIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERPTHR11214. PTHR11214. 1 hit.
PfamPF01762. Galactosyl_T. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSB3GNT2. mouse.
NextBio310369.
PROQ9Z222.
SOURCESearch...

Entry information

Entry nameB3GN2_MOUSE
AccessionPrimary (citable) accession number: Q9Z222
Secondary accession number(s): Q91V18
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: October 25, 2004
Last modified: July 9, 2014
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot