UniProtKB - Q9Z214 (HOME1_RAT)
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Protein
Homer protein homolog 1
Gene
Homer1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Differentially regulates the functions of the calcium activated channel ryanodine receptors RYR1 and RYR2. Isoform 1 decreases the activity of RYR2, and increases the activity of RYR1, whereas isoform 3 counteracts the effects by competing for binding sites. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1, and isoform 2 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development.1 Publication
GO - Molecular functioni
- G-protein coupled glutamate receptor binding Source: UniProtKB
- identical protein binding Source: IntAct
- ion channel binding Source: RGD
- protein-containing complex scaffold activity Source: BHF-UCL
- protein heterodimerization activity Source: RGD
- receptor binding Source: RGD
- scaffold protein binding Source: BHF-UCL
- type 5 metabotropic glutamate receptor binding Source: RGD
GO - Biological processi
- behavioral response to cocaine Source: RGD
- chemical homeostasis within a tissue Source: RGD
- circadian rhythm Source: RGD
- G-protein coupled glutamate receptor signaling pathway Source: RGD
- positive regulation of calcium ion transport Source: RGD
- protein localization to synapse Source: BHF-UCL
- regulation of calcium ion import Source: RGD
- regulation of cation channel activity Source: RGD
- regulation of store-operated calcium entry Source: RGD
- response to calcium ion Source: RGD
- response to cocaine Source: RGD
- response to nicotine Source: RGD
- skeletal muscle contraction Source: RGD
- skeletal muscle fiber development Source: RGD
Enzyme and pathway databases
Reactomei | R-RNO-6794361 Neurexins and neuroligins |
Names & Taxonomyi
Protein namesi | Recommended name: Homer protein homolog 1Alternative name(s): PSD-Zip45 VASP/Ena-related gene up-regulated during seizure and LTP 1 Short name: Vesl-1 |
Gene namesi | Name:Homer1 Synonyms:Homer, Vesl |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 628725 Homer1 |
Subcellular locationi
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, Membrane, Postsynaptic cell membrane, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 24 | W → A: Disrupts binding to both GRM1 and SHANK3. 1 Publication | 1 | |
Mutagenesisi | 24 | W → Y: Disrupts binding to GRM1. 1 Publication | 1 | |
Mutagenesisi | 70 | T → A: Normal binding. 1 Publication | 1 | |
Mutagenesisi | 70 | T → E: Disrupts binding to SHANK3. 1 Publication | 1 | |
Mutagenesisi | 74 | F → A: Eliminates binding to both GRM1 and SHANK3. 1 Publication | 1 | |
Mutagenesisi | 76 | Q → A: Normal binding. 1 Publication | 1 | |
Mutagenesisi | 76 | Q → R: Normal binding. 1 Publication | 1 | |
Mutagenesisi | 85 | V → A: Diminishes binding to GRM1. 1 Publication | 1 | |
Mutagenesisi | 89 | G → A: Eliminates binding to both GRM1 and SHANK3. 1 Publication | 1 | |
Mutagenesisi | 89 | G → N: Eliminates binding to both GRM1 and SHANK3. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000191007 | 2 – 366 | Homer protein homolog 1Add BLAST | 365 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylglycineBy similarity | 1 | |
Modified residuei | 318 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q9Z214 |
PRIDEi | Q9Z214 |
PTM databases
iPTMneti | Q9Z214 |
PhosphoSitePlusi | Q9Z214 |
Expressioni
Tissue specificityi
Highly expressed in cortex, Purkinje cells of the cerebellum, hippocampus, striatum and olfactory bulb. Isoform 1 and isoform 3 are expressed in skeletal and cardiac muscle.1 Publication
Developmental stagei
In the developing hippocampus, the expression of isoform 1 is high at P8, then decreased with progression of hippocampal development. Isoform 3 expression was constitutively low, and not regulated during hippocampal development.
Inductioni
Isoform 3 is induced in the hippocampus, by seizure and synaptic mechanisms in association with long-term potentiation (LTP). It is also induced in the striatum by drugs that alter dopamine signaling.
Gene expression databases
Bgeei | ENSRNOG00000047014 |
ExpressionAtlasi | Q9Z214 baseline and differential |
Genevisiblei | Q9Z214 RN |
Interactioni
Subunit structurei
Interacts with GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. Interacts with IFT57 and OPHN1. Isoform 1 and isoform 2 encode coiled-coil structures that mediate homo- and heteromultimerization.10 Publications
Binary interactionsi
GO - Molecular functioni
- G-protein coupled glutamate receptor binding Source: UniProtKB
- identical protein binding Source: IntAct
- ion channel binding Source: RGD
- protein-containing complex scaffold activity Source: BHF-UCL
- protein heterodimerization activity Source: RGD
- receptor binding Source: RGD
- scaffold protein binding Source: BHF-UCL
- type 5 metabotropic glutamate receptor binding Source: RGD
Protein-protein interaction databases
BioGridi | 24818011 interactors. |
ELMi | Q9Z214 |
IntActi | Q9Z214 9 interactors. |
MINTi | Q9Z214 |
STRINGi | 10116.ENSRNOP00000065989 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Beta strandi | 3 – 16 | Combined sources | 14 | |
Turni | 18 – 20 | Combined sources | 3 | |
Beta strandi | 23 – 27 | Combined sources | 5 | |
Beta strandi | 32 – 39 | Combined sources | 8 | |
Turni | 40 – 43 | Combined sources | 4 | |
Beta strandi | 44 – 51 | Combined sources | 8 | |
Beta strandi | 54 – 60 | Combined sources | 7 | |
Beta strandi | 71 – 79 | Combined sources | 9 | |
Turni | 80 – 83 | Combined sources | 4 | |
Beta strandi | 84 – 89 | Combined sources | 6 | |
Helixi | 93 – 110 | Combined sources | 18 | |
Helixi | 302 – 362 | Combined sources | 61 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DDV | X-ray | 1.90 | A | 1-111 | [»] | |
1DDW | X-ray | 1.70 | A | 1-120 | [»] | |
1I2H | X-ray | 1.80 | A | 1-163 | [»] | |
3CVE | X-ray | 1.75 | A/B/C/D | 302-366 | [»] | |
ProteinModelPortali | Q9Z214 | |||||
SMRi | Q9Z214 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9Z214 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 110 | WH1PROSITE-ProRule annotationAdd BLAST | 110 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 193 – 364 | Sequence analysisAdd BLAST | 172 |
Domaini
The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.
Sequence similaritiesi
Belongs to the Homer family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG410IGRQ Eukaryota ENOG410XQWT LUCA |
GeneTreei | ENSGT00390000017850 |
HOVERGENi | HBG051918 |
InParanoidi | Q9Z214 |
KOi | K15010 |
OrthoDBi | EOG091G0CQ0 |
PhylomeDBi | Q9Z214 |
Family and domain databases
Gene3Di | 2.30.29.301 hit |
InterProi | View protein in InterPro IPR011993 PH-like_dom_sf IPR000697 WH1/EVH1_dom |
Pfami | View protein in Pfam PF00568 WH1, 1 hit |
SMARTi | View protein in SMART SM00461 WH1, 1 hit |
PROSITEi | View protein in PROSITE PS50229 WH1, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9Z214-1) [UniParc]FASTAAdd to basket
Also known as: 1c, Vesl-1L
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MGEQPIFSTR AHVFQIDPNT KKNWVPTSKH AVTVSYFYDS TRNVYRIISL
60 70 80 90 100
DGSKAIINST ITPNMTFTKT SQKFGQWADS RANTVYGLGF SSEHHLSKFA
110 120 130 140 150
EKFQEFKEAA RLAKEKSQEK MELTSTPSQE SAGGDLQSPL TPESINGTDD
160 170 180 190 200
ERTPDVTQNS EPRAEPAQNA LPFSHSAGDR TQGLSHASSA ISKHWEAELA
210 220 230 240 250
TLKGNNAKLT AALLESTANV KQWKQQLAAY QEEAERLHKR VTELECVSSQ
260 270 280 290 300
ANAVHSHKTE LSQTVQELEE TLKVKEEEIE RLKQEIDNAR ELQEQRDSLT
310 320 330 340 350
QKLQEVEIRN KDLEGQLSEL EQRLEKSQSE QDAFRSNLKT LLEILDGKIF
360
ELTELRDNLA KLLECS
Sequence cautioni
The sequence AAC53113 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 268 | L → R in BAA32477 (PubMed:9727012).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009066 | 176 – 187 | Missing in isoform 2. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_009067 | 176 – 186 | SAGDRTQGLSH → RYTFNSAIMIK in isoform 3. 5 PublicationsAdd BLAST | 11 | |
Alternative sequenceiVSP_009068 | 187 – 366 | Missing in isoform 3. 5 PublicationsAdd BLAST | 180 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U92079 mRNA Translation: AAC53113.1 Different initiation. AB003726 mRNA Translation: BAA21671.1 AF093267 mRNA Translation: AAC71031.1 AF093268 mRNA Translation: AAC71032.1 AB017140 mRNA Translation: BAA34311.1 AB007688 mRNA Translation: BAA32477.1 AJ276327 mRNA Translation: CAB77249.1 AJ276328 mRNA Translation: CAB77250.1 |
RefSeqi | NP_113895.1, NM_031707.1 [Q9Z214-1] XP_006231837.1, XM_006231775.3 [Q9Z214-2] |
UniGenei | Rn.37500 |
Genome annotation databases
Ensembli | ENSRNOT00000073871; ENSRNOP00000066634; ENSRNOG00000047014 [Q9Z214-2] |
GeneIDi | 29546 |
KEGGi | rno:29546 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Entry informationi
Entry namei | HOME1_RAT | |
Accessioni | Q9Z214Primary (citable) accession number: Q9Z214 Secondary accession number(s): O08567 Q9QWN5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2003 |
Last sequence update: | November 28, 2003 | |
Last modified: | April 25, 2018 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |