Q9Z205 (RFXK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-binding protein RFXANK

Alternative name(s):
Ankyrin repeat-containing adapter protein Tvl-1
Regulatory factor X subunit B
Short name=RFX-B
Regulatory factor X-associated ankyrin-containing protein

Gene names

Name:	Rfxank
Synonyms:	Rfxb, Tvl1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator (CIITA). May regulate other genes in the cell By similarity. Potentiates the activation of RAF-1. RFX binds the X1 box of MHC-II promoters.
Subunit structure	The RFX heterotetrameric complex consists of 2 molecules of RFX5 and one each of RFXAP and RFX-B/RFXANK; with each subunit representing a separate complementation group. RFX forms cooperative DNA binding complexes with X2BP and CBF/NF-Y. RFX associates with CIITA to form an active transcriptional complex By similarity. Forms oligomers. Associates with raf-1.
Subcellular location	Cytoplasm. Nucleus.
Tissue specificity	Expressed primarily in thymus, lung and testis.
Domain	Interacts with RAF-1 via its C-terminal ankyrin repeat domain. The same domain also mediates its homodimerization. The third ankyrin repeat is required for association with the two other RFX subunits; RFX5 and RFXAP. The three central ANK repeats mediate binding to the PxLPxI/L motif of RFX5 By similarity.
Post-translational modification	Phosphorylated by RAF-1.
Sequence similarities	Contains 5 ANK repeats.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	ANK repeat Repeat
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Ras protein signal transduction Inferred from physical interaction Ref.2. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 16236793. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay Ref.2. Source: MGI nucleus Inferred from direct assay Ref.2. Source: MGI
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW histone deacetylase binding Inferred from direct assay PubMed 16236793. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q9Z205-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q9Z205-2)

The sequence of this isoform differs from the canonical sequence as follows:
     112-121: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	DNA-binding protein RFXANK		PRO_0000067050
Regions
	Repeat	88 – 127	40	ANK 1
	Repeat	132 – 161	30	ANK 2
	Repeat	165 – 194	30	ANK 3
	Repeat	198 – 227	30	ANK 4
	Repeat	231 – 260	30	ANK 5
Natural variations
	Alternative sequence	112 – 121	10	Missing in isoform Short.		VSP_000285

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 5713F335DC669E87	FASTA	269	29,232
	10 20 30 40 50 60 MEPTQVAENL VPNQQPPVPD LEDPEDTRDE SPENSDTVVL SLFPCTPDAV NPEADASASS 70 80 90 100 110 120 LQGSFLKHST TLTNRQRGNE VSALPATLDS LSIHQLAAQG ELSQLKDHLR KGACPACTCL 130 140 150 160 170 180 SGNNLINKPD ERGFTPLIWA SAFGEIETVR FLLDWGADPH ILAKERESAL SLASMGGYTD 190 200 210 220 230 240 IVRLLLDRDV DINIYDWNGG TPLLYAVRGN HVKCVEALLA RGADLTTEAD SGYTPMDLAV 250 260 ALGYRKVQQV MESHILRLFQ STLGPVDPE
	Isoform Short [UniParc]. Checksum: FC0168DC986D95C9	FASTA	259	28,325
	10 20 30 40 50 60 MEPTQVAENL VPNQQPPVPD LEDPEDTRDE SPENSDTVVL SLFPCTPDAV NPEADASASS 70 80 90 100 110 120 LQGSFLKHST TLTNRQRGNE VSALPATLDS LSIHQLAAQG ELSQLKDHLR KGNNLINKPD 130 140 150 160 170 180 ERGFTPLIWA SAFGEIETVR FLLDWGADPH ILAKERESAL SLASMGGYTD IVRLLLDRDV 190 200 210 220 230 240 DINIYDWNGG TPLLYAVRGN HVKCVEALLA RGADLTTEAD SGYTPMDLAV ALGYRKVQQV 250 MESHILRLFQ STLGPVDPE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene encoding a novel RFX-associated transactivator is mutated in the majority of MHC class II deficiency patients." Masternak K., Barras E., Zufferey M., Conrad B., Corthals G., Aebersold R., Sanchez J.-C., Hochstrasser D.F., Mach B., Reith W. Nat. Genet. 20:273-277(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Spleen.
[2]	"The ankyrin repeat-containing adaptor protein tvl-1 is a novel substrate and regulator of raf-1." Lin J.-H., Makris A., McMahon C., Bear S.E., Patriotis C., Prasad V.R., Brent R., Golemis E.A., Tsichlis P.N. J. Biol. Chem. 274:14706-14715(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: T-cell.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT). Strain: FVB/N. Tissue: Salivary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF094761 mRNA. Translation: AAC69884.1. AF123704 mRNA. Translation: AAD24798.1. BC010971 mRNA. Translation: AAH10971.1.
IPI	IPI00130345. IPI00279247.
RefSeq	NP_001020760.1. NM_001025589.1. NP_035396.1. NM_011266.2.
UniGene	Mm.161167.
3D structure databases
ProteinModelPortal	Q9Z205.
SMR	Q9Z205. Positions 50-261.
ModBase	Search...
PTM databases
PhosphoSite	Q9Z205.
Proteomic databases
PRIDE	Q9Z205.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000075724; ENSMUSP00000075140; ENSMUSG00000036120.
GeneID	19727.
KEGG	mmu:19727.
Organism-specific databases
CTD	8625.
MGI	MGI:1333865. Rfxank.
Phylogenomic databases
eggNOG	COG0666.
GeneTree	ENSGT00390000009838.
HOGENOM	HOG000294108.
HOVERGEN	HBG017718.
InParanoid	Q9Z205.
KO	K08062.
OMA	CTPEPGN.
OrthoDB	EOG42822S.
Gene expression databases
ArrayExpress	Q9Z205.
Bgee	Q9Z205.
Genevestigator	Q9Z205.
GermOnline	ENSMUSG00000036120. Mus musculus.
Family and domain databases
Gene3D	1.25.40.20. 1 hit.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR017362. DNA-bd_RFXANK. [Graphical view]
Pfam	PF00023. Ank. 1 hit. PF12796. Ank_2. 1 hit. [Graphical view]
PIRSF	PIRSF038034. DNA-binding_RFXANK. 1 hit.
SMART	SM00248. ANK. 4 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	297140.
SOURCE	Search...

Entry information

Entry name

RFXK_MOUSE

Accession

Primary (citable) accession number: Q9Z205

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program