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Q9Z205

- RFXK_MOUSE

UniProt

Q9Z205 - RFXK_MOUSE

Protein

DNA-binding protein RFXANK

Gene

Rfxank

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator (CIITA). May regulate other genes in the cell By similarity. Potentiates the activation of RAF-1. RFX binds the X1 box of MHC-II promoters.By similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. histone deacetylase binding Source: MGI
    3. protein binding Source: MGI

    GO - Biological processi

    1. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    2. Ras protein signal transduction Source: MGI
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-binding protein RFXANK
    Alternative name(s):
    Ankyrin repeat-containing adapter protein Tvl-1
    Regulatory factor X subunit B
    Short name:
    RFX-B
    Regulatory factor X-associated ankyrin-containing protein
    Gene namesi
    Name:Rfxank
    Synonyms:Rfxb, Tvl1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1333865. Rfxank.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 269269DNA-binding protein RFXANKPRO_0000067050Add
    BLAST

    Post-translational modificationi

    Phosphorylated by RAF-1.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ9Z205.

    PTM databases

    PhosphoSiteiQ9Z205.

    Expressioni

    Tissue specificityi

    Expressed primarily in thymus, lung and testis.

    Gene expression databases

    ArrayExpressiQ9Z205.
    BgeeiQ9Z205.
    GenevestigatoriQ9Z205.

    Interactioni

    Subunit structurei

    The RFX heterotetrameric complex consists of 2 molecules of RFX5 and one each of RFXAP and RFX-B/RFXANK; with each subunit representing a separate complementation group. RFX forms cooperative DNA binding complexes with X2BP and CBF/NF-Y. RFX associates with CIITA to form an active transcriptional complex By similarity. Forms oligomers. Associates with raf-1.By similarity

    Protein-protein interaction databases

    BioGridi202875. 7 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z205.
    SMRiQ9Z205. Positions 50-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati88 – 12740ANK 1Add
    BLAST
    Repeati132 – 16130ANK 2Add
    BLAST
    Repeati165 – 19430ANK 3Add
    BLAST
    Repeati198 – 22730ANK 4Add
    BLAST
    Repeati231 – 26030ANK 5Add
    BLAST

    Domaini

    Interacts with RAF-1 via its C-terminal ankyrin repeat domain. The same domain also mediates its homodimerization.
    The third ankyrin repeat is required for association with the two other RFX subunits; RFX5 and RFXAP. The three central ANK repeats mediate binding to the PxLPxI/L motif of RFX5 By similarity.By similarity

    Sequence similaritiesi

    Contains 5 ANK repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Repeat

    Phylogenomic databases

    eggNOGiCOG0666.
    GeneTreeiENSGT00730000110740.
    HOGENOMiHOG000294108.
    HOVERGENiHBG017718.
    InParanoidiQ9Z205.
    KOiK08062.
    OMAiMEPTQPA.
    OrthoDBiEOG7WMCJS.
    PhylomeDBiQ9Z205.
    TreeFamiTF333112.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR017362. DNA-bd_RFXANK.
    [Graphical view]
    PfamiPF00023. Ank. 1 hit.
    PF12796. Ank_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038034. DNA-binding_RFXANK. 1 hit.
    SMARTiSM00248. ANK. 4 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 3 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: Q9Z205-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEPTQVAENL VPNQQPPVPD LEDPEDTRDE SPENSDTVVL SLFPCTPDAV    50
    NPEADASASS LQGSFLKHST TLTNRQRGNE VSALPATLDS LSIHQLAAQG 100
    ELSQLKDHLR KGACPACTCL SGNNLINKPD ERGFTPLIWA SAFGEIETVR 150
    FLLDWGADPH ILAKERESAL SLASMGGYTD IVRLLLDRDV DINIYDWNGG 200
    TPLLYAVRGN HVKCVEALLA RGADLTTEAD SGYTPMDLAV ALGYRKVQQV 250
    MESHILRLFQ STLGPVDPE 269
    Length:269
    Mass (Da):29,232
    Last modified:May 1, 1999 - v1
    Checksum:i5713F335DC669E87
    GO
    Isoform Short (identifier: Q9Z205-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         112-121: Missing.

    Show »
    Length:259
    Mass (Da):28,325
    Checksum:iFC0168DC986D95C9
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei112 – 12110Missing in isoform Short. 1 PublicationVSP_000285

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF094761 mRNA. Translation: AAC69884.1.
    AF123704 mRNA. Translation: AAD24798.1.
    BC010971 mRNA. Translation: AAH10971.1.
    CCDSiCCDS22360.1. [Q9Z205-1]
    RefSeqiNP_001020760.1. NM_001025589.1. [Q9Z205-2]
    NP_035396.1. NM_011266.2. [Q9Z205-1]
    UniGeneiMm.161167.

    Genome annotation databases

    EnsembliENSMUST00000075724; ENSMUSP00000075140; ENSMUSG00000036120. [Q9Z205-1]
    GeneIDi19727.
    KEGGimmu:19727.
    UCSCiuc009lyt.1. mouse. [Q9Z205-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF094761 mRNA. Translation: AAC69884.1 .
    AF123704 mRNA. Translation: AAD24798.1 .
    BC010971 mRNA. Translation: AAH10971.1 .
    CCDSi CCDS22360.1. [Q9Z205-1 ]
    RefSeqi NP_001020760.1. NM_001025589.1. [Q9Z205-2 ]
    NP_035396.1. NM_011266.2. [Q9Z205-1 ]
    UniGenei Mm.161167.

    3D structure databases

    ProteinModelPortali Q9Z205.
    SMRi Q9Z205. Positions 50-261.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202875. 7 interactions.

    PTM databases

    PhosphoSitei Q9Z205.

    Proteomic databases

    PRIDEi Q9Z205.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000075724 ; ENSMUSP00000075140 ; ENSMUSG00000036120 . [Q9Z205-1 ]
    GeneIDi 19727.
    KEGGi mmu:19727.
    UCSCi uc009lyt.1. mouse. [Q9Z205-1 ]

    Organism-specific databases

    CTDi 8625.
    MGIi MGI:1333865. Rfxank.

    Phylogenomic databases

    eggNOGi COG0666.
    GeneTreei ENSGT00730000110740.
    HOGENOMi HOG000294108.
    HOVERGENi HBG017718.
    InParanoidi Q9Z205.
    KOi K08062.
    OMAi MEPTQPA.
    OrthoDBi EOG7WMCJS.
    PhylomeDBi Q9Z205.
    TreeFami TF333112.

    Miscellaneous databases

    NextBioi 297140.
    PROi Q9Z205.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Z205.
    Bgeei Q9Z205.
    Genevestigatori Q9Z205.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR017362. DNA-bd_RFXANK.
    [Graphical view ]
    Pfami PF00023. Ank. 1 hit.
    PF12796. Ank_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038034. DNA-binding_RFXANK. 1 hit.
    SMARTi SM00248. ANK. 4 hits.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A gene encoding a novel RFX-associated transactivator is mutated in the majority of MHC class II deficiency patients."
      Masternak K., Barras E., Zufferey M., Conrad B., Corthals G., Aebersold R., Sanchez J.-C., Hochstrasser D.F., Mach B., Reith W.
      Nat. Genet. 20:273-277(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Spleen.
    2. "The ankyrin repeat-containing adaptor protein tvl-1 is a novel substrate and regulator of raf-1."
      Lin J.-H., Makris A., McMahon C., Bear S.E., Patriotis C., Prasad V.R., Brent R., Golemis E.A., Tsichlis P.N.
      J. Biol. Chem. 274:14706-14715(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: T-cell.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
      Strain: FVB/N.
      Tissue: Salivary gland.

    Entry informationi

    Entry nameiRFXK_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z205
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3