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Q9Z205

- RFXK_MOUSE

UniProt

Q9Z205 - RFXK_MOUSE

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Protein

DNA-binding protein RFXANK

Gene
Rfxank, Rfxb, Tvl1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator (CIITA). May regulate other genes in the cell By similarity. Potentiates the activation of RAF-1. RFX binds the X1 box of MHC-II promoters.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. histone deacetylase binding Source: MGI
  3. protein binding Source: MGI

GO - Biological processi

  1. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  2. Ras protein signal transduction Source: MGI
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein RFXANK
Alternative name(s):
Ankyrin repeat-containing adapter protein Tvl-1
Regulatory factor X subunit B
Short name:
RFX-B
Regulatory factor X-associated ankyrin-containing protein
Gene namesi
Name:Rfxank
Synonyms:Rfxb, Tvl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1333865. Rfxank.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269DNA-binding protein RFXANKPRO_0000067050Add
BLAST

Post-translational modificationi

Phosphorylated by RAF-1.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9Z205.

PTM databases

PhosphoSiteiQ9Z205.

Expressioni

Tissue specificityi

Expressed primarily in thymus, lung and testis.

Gene expression databases

ArrayExpressiQ9Z205.
BgeeiQ9Z205.
GenevestigatoriQ9Z205.

Interactioni

Subunit structurei

The RFX heterotetrameric complex consists of 2 molecules of RFX5 and one each of RFXAP and RFX-B/RFXANK; with each subunit representing a separate complementation group. RFX forms cooperative DNA binding complexes with X2BP and CBF/NF-Y. RFX associates with CIITA to form an active transcriptional complex By similarity. Forms oligomers. Associates with raf-1.

Protein-protein interaction databases

BioGridi202875. 7 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9Z205.
SMRiQ9Z205. Positions 50-261.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati88 – 12740ANK 1Add
BLAST
Repeati132 – 16130ANK 2Add
BLAST
Repeati165 – 19430ANK 3Add
BLAST
Repeati198 – 22730ANK 4Add
BLAST
Repeati231 – 26030ANK 5Add
BLAST

Domaini

Interacts with RAF-1 via its C-terminal ankyrin repeat domain. The same domain also mediates its homodimerization.
The third ankyrin repeat is required for association with the two other RFX subunits; RFX5 and RFXAP. The three central ANK repeats mediate binding to the PxLPxI/L motif of RFX5 By similarity.

Sequence similaritiesi

Contains 5 ANK repeats.

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00730000110740.
HOGENOMiHOG000294108.
HOVERGENiHBG017718.
InParanoidiQ9Z205.
KOiK08062.
OMAiMEPTQPA.
OrthoDBiEOG7WMCJS.
PhylomeDBiQ9Z205.
TreeFamiTF333112.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017362. DNA-bd_RFXANK.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
PF12796. Ank_2. 1 hit.
[Graphical view]
PIRSFiPIRSF038034. DNA-binding_RFXANK. 1 hit.
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: Q9Z205-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEPTQVAENL VPNQQPPVPD LEDPEDTRDE SPENSDTVVL SLFPCTPDAV    50
NPEADASASS LQGSFLKHST TLTNRQRGNE VSALPATLDS LSIHQLAAQG 100
ELSQLKDHLR KGACPACTCL SGNNLINKPD ERGFTPLIWA SAFGEIETVR 150
FLLDWGADPH ILAKERESAL SLASMGGYTD IVRLLLDRDV DINIYDWNGG 200
TPLLYAVRGN HVKCVEALLA RGADLTTEAD SGYTPMDLAV ALGYRKVQQV 250
MESHILRLFQ STLGPVDPE 269
Length:269
Mass (Da):29,232
Last modified:May 1, 1999 - v1
Checksum:i5713F335DC669E87
GO
Isoform Short (identifier: Q9Z205-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     112-121: Missing.

Show »
Length:259
Mass (Da):28,325
Checksum:iFC0168DC986D95C9
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei112 – 12110Missing in isoform Short. VSP_000285

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF094761 mRNA. Translation: AAC69884.1.
AF123704 mRNA. Translation: AAD24798.1.
BC010971 mRNA. Translation: AAH10971.1.
CCDSiCCDS22360.1. [Q9Z205-1]
RefSeqiNP_001020760.1. NM_001025589.1. [Q9Z205-2]
NP_035396.1. NM_011266.2. [Q9Z205-1]
UniGeneiMm.161167.

Genome annotation databases

EnsembliENSMUST00000075724; ENSMUSP00000075140; ENSMUSG00000036120. [Q9Z205-1]
GeneIDi19727.
KEGGimmu:19727.
UCSCiuc009lyt.1. mouse. [Q9Z205-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF094761 mRNA. Translation: AAC69884.1 .
AF123704 mRNA. Translation: AAD24798.1 .
BC010971 mRNA. Translation: AAH10971.1 .
CCDSi CCDS22360.1. [Q9Z205-1 ]
RefSeqi NP_001020760.1. NM_001025589.1. [Q9Z205-2 ]
NP_035396.1. NM_011266.2. [Q9Z205-1 ]
UniGenei Mm.161167.

3D structure databases

ProteinModelPortali Q9Z205.
SMRi Q9Z205. Positions 50-261.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202875. 7 interactions.

PTM databases

PhosphoSitei Q9Z205.

Proteomic databases

PRIDEi Q9Z205.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000075724 ; ENSMUSP00000075140 ; ENSMUSG00000036120 . [Q9Z205-1 ]
GeneIDi 19727.
KEGGi mmu:19727.
UCSCi uc009lyt.1. mouse. [Q9Z205-1 ]

Organism-specific databases

CTDi 8625.
MGIi MGI:1333865. Rfxank.

Phylogenomic databases

eggNOGi COG0666.
GeneTreei ENSGT00730000110740.
HOGENOMi HOG000294108.
HOVERGENi HBG017718.
InParanoidi Q9Z205.
KOi K08062.
OMAi MEPTQPA.
OrthoDBi EOG7WMCJS.
PhylomeDBi Q9Z205.
TreeFami TF333112.

Miscellaneous databases

NextBioi 297140.
PROi Q9Z205.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9Z205.
Bgeei Q9Z205.
Genevestigatori Q9Z205.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017362. DNA-bd_RFXANK.
[Graphical view ]
Pfami PF00023. Ank. 1 hit.
PF12796. Ank_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF038034. DNA-binding_RFXANK. 1 hit.
SMARTi SM00248. ANK. 4 hits.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A gene encoding a novel RFX-associated transactivator is mutated in the majority of MHC class II deficiency patients."
    Masternak K., Barras E., Zufferey M., Conrad B., Corthals G., Aebersold R., Sanchez J.-C., Hochstrasser D.F., Mach B., Reith W.
    Nat. Genet. 20:273-277(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Spleen.
  2. "The ankyrin repeat-containing adaptor protein tvl-1 is a novel substrate and regulator of raf-1."
    Lin J.-H., Makris A., McMahon C., Bear S.E., Patriotis C., Prasad V.R., Brent R., Golemis E.A., Tsichlis P.N.
    J. Biol. Chem. 274:14706-14715(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: T-cell.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
    Strain: FVB/N.
    Tissue: Salivary gland.

Entry informationi

Entry nameiRFXK_MOUSE
AccessioniPrimary (citable) accession number: Q9Z205
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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