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Protein

DNA-binding protein RFXANK

Gene

Rfxank

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator (CIITA). May regulate other genes in the cell (By similarity). Potentiates the activation of RAF-1. RFX binds the X1 box of MHC-II promoters.By similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. histone deacetylase binding Source: MGI
  3. transcription cofactor activity Source: GO_Central

GO - Biological processi

  1. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  2. Ras protein signal transduction Source: MGI
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein RFXANK
Alternative name(s):
Ankyrin repeat-containing adapter protein Tvl-1
Regulatory factor X subunit B
Short name:
RFX-B
Regulatory factor X-associated ankyrin-containing protein
Gene namesi
Name:Rfxank
Synonyms:Rfxb, Tvl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1333865. Rfxank.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. intercellular bridge Source: MGI
  3. nucleoplasm Source: MGI
  4. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269DNA-binding protein RFXANKPRO_0000067050Add
BLAST

Post-translational modificationi

Phosphorylated by RAF-1.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z205.
PRIDEiQ9Z205.

PTM databases

PhosphoSiteiQ9Z205.

Expressioni

Tissue specificityi

Expressed primarily in thymus, lung and testis.

Gene expression databases

BgeeiQ9Z205.
ExpressionAtlasiQ9Z205. baseline and differential.
GenevestigatoriQ9Z205.

Interactioni

Subunit structurei

The RFX heterotetrameric complex consists of 2 molecules of RFX5 and one each of RFXAP and RFX-B/RFXANK; with each subunit representing a separate complementation group. RFX forms cooperative DNA binding complexes with X2BP and CBF/NF-Y. RFX associates with CIITA to form an active transcriptional complex (By similarity). Forms oligomers. Associates with raf-1.By similarity

Protein-protein interaction databases

BioGridi202875. 7 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9Z205.
SMRiQ9Z205. Positions 50-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati88 – 12740ANK 1Add
BLAST
Repeati132 – 16130ANK 2Add
BLAST
Repeati165 – 19430ANK 3Add
BLAST
Repeati198 – 22730ANK 4Add
BLAST
Repeati231 – 26030ANK 5Add
BLAST

Domaini

Interacts with RAF-1 via its C-terminal ankyrin repeat domain. The same domain also mediates its homodimerization.
The third ankyrin repeat is required for association with the two other RFX subunits; RFX5 and RFXAP. The three central ANK repeats mediate binding to the PxLPxI/L motif of RFX5 (By similarity).By similarity

Sequence similaritiesi

Contains 5 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00730000110740.
HOGENOMiHOG000294108.
HOVERGENiHBG017718.
InParanoidiQ9Z205.
KOiK08062.
OMAiMEPTQPA.
OrthoDBiEOG7WMCJS.
PhylomeDBiQ9Z205.
TreeFamiTF333112.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017362. DNA-bd_RFXANK.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
PF12796. Ank_2. 1 hit.
[Graphical view]
PIRSFiPIRSF038034. DNA-binding_RFXANK. 1 hit.
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q9Z205-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPTQVAENL VPNQQPPVPD LEDPEDTRDE SPENSDTVVL SLFPCTPDAV
60 70 80 90 100
NPEADASASS LQGSFLKHST TLTNRQRGNE VSALPATLDS LSIHQLAAQG
110 120 130 140 150
ELSQLKDHLR KGACPACTCL SGNNLINKPD ERGFTPLIWA SAFGEIETVR
160 170 180 190 200
FLLDWGADPH ILAKERESAL SLASMGGYTD IVRLLLDRDV DINIYDWNGG
210 220 230 240 250
TPLLYAVRGN HVKCVEALLA RGADLTTEAD SGYTPMDLAV ALGYRKVQQV
260
MESHILRLFQ STLGPVDPE
Length:269
Mass (Da):29,232
Last modified:April 30, 1999 - v1
Checksum:i5713F335DC669E87
GO
Isoform Short (identifier: Q9Z205-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     112-121: Missing.

Show »
Length:259
Mass (Da):28,325
Checksum:iFC0168DC986D95C9
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei112 – 12110Missing in isoform Short. 1 PublicationVSP_000285

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF094761 mRNA. Translation: AAC69884.1.
AF123704 mRNA. Translation: AAD24798.1.
BC010971 mRNA. Translation: AAH10971.1.
CCDSiCCDS22360.1. [Q9Z205-1]
RefSeqiNP_001020760.1. NM_001025589.1. [Q9Z205-2]
NP_035396.1. NM_011266.2. [Q9Z205-1]
UniGeneiMm.161167.

Genome annotation databases

EnsembliENSMUST00000075724; ENSMUSP00000075140; ENSMUSG00000036120. [Q9Z205-1]
GeneIDi19727.
KEGGimmu:19727.
UCSCiuc009lyt.1. mouse. [Q9Z205-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF094761 mRNA. Translation: AAC69884.1.
AF123704 mRNA. Translation: AAD24798.1.
BC010971 mRNA. Translation: AAH10971.1.
CCDSiCCDS22360.1. [Q9Z205-1]
RefSeqiNP_001020760.1. NM_001025589.1. [Q9Z205-2]
NP_035396.1. NM_011266.2. [Q9Z205-1]
UniGeneiMm.161167.

3D structure databases

ProteinModelPortaliQ9Z205.
SMRiQ9Z205. Positions 50-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202875. 7 interactions.

PTM databases

PhosphoSiteiQ9Z205.

Proteomic databases

MaxQBiQ9Z205.
PRIDEiQ9Z205.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075724; ENSMUSP00000075140; ENSMUSG00000036120. [Q9Z205-1]
GeneIDi19727.
KEGGimmu:19727.
UCSCiuc009lyt.1. mouse. [Q9Z205-1]

Organism-specific databases

CTDi8625.
MGIiMGI:1333865. Rfxank.

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00730000110740.
HOGENOMiHOG000294108.
HOVERGENiHBG017718.
InParanoidiQ9Z205.
KOiK08062.
OMAiMEPTQPA.
OrthoDBiEOG7WMCJS.
PhylomeDBiQ9Z205.
TreeFamiTF333112.

Miscellaneous databases

NextBioi297140.
PROiQ9Z205.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z205.
ExpressionAtlasiQ9Z205. baseline and differential.
GenevestigatoriQ9Z205.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017362. DNA-bd_RFXANK.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
PF12796. Ank_2. 1 hit.
[Graphical view]
PIRSFiPIRSF038034. DNA-binding_RFXANK. 1 hit.
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A gene encoding a novel RFX-associated transactivator is mutated in the majority of MHC class II deficiency patients."
    Masternak K., Barras E., Zufferey M., Conrad B., Corthals G., Aebersold R., Sanchez J.-C., Hochstrasser D.F., Mach B., Reith W.
    Nat. Genet. 20:273-277(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Spleen.
  2. "The ankyrin repeat-containing adaptor protein tvl-1 is a novel substrate and regulator of raf-1."
    Lin J.-H., Makris A., McMahon C., Bear S.E., Patriotis C., Prasad V.R., Brent R., Golemis E.A., Tsichlis P.N.
    J. Biol. Chem. 274:14706-14715(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: T-cell.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
    Strain: FVB/N.
    Tissue: Salivary gland.

Entry informationi

Entry nameiRFXK_MOUSE
AccessioniPrimary (citable) accession number: Q9Z205
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2000
Last sequence update: April 30, 1999
Last modified: March 3, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.