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Q9Z205 (RFXK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-binding protein RFXANK
Alternative name(s):
Ankyrin repeat-containing adapter protein Tvl-1
Regulatory factor X subunit B
Short name=RFX-B
Regulatory factor X-associated ankyrin-containing protein
Gene names
Name:Rfxank
Synonyms:Rfxb, Tvl1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length269 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator (CIITA). May regulate other genes in the cell By similarity. Potentiates the activation of RAF-1. RFX binds the X1 box of MHC-II promoters.

Subunit structure

The RFX heterotetrameric complex consists of 2 molecules of RFX5 and one each of RFXAP and RFX-B/RFXANK; with each subunit representing a separate complementation group. RFX forms cooperative DNA binding complexes with X2BP and CBF/NF-Y. RFX associates with CIITA to form an active transcriptional complex By similarity. Forms oligomers. Associates with raf-1.

Subcellular location

Cytoplasm. Nucleus.

Tissue specificity

Expressed primarily in thymus, lung and testis.

Domain

Interacts with RAF-1 via its C-terminal ankyrin repeat domain. The same domain also mediates its homodimerization.

The third ankyrin repeat is required for association with the two other RFX subunits; RFX5 and RFXAP. The three central ANK repeats mediate binding to the PxLPxI/L motif of RFX5 By similarity.

Post-translational modification

Phosphorylated by RAF-1.

Sequence similarities

Contains 5 ANK repeats.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q9Z205-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q9Z205-2)

The sequence of this isoform differs from the canonical sequence as follows:
     112-121: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 269269DNA-binding protein RFXANK
PRO_0000067050

Regions

Repeat88 – 12740ANK 1
Repeat132 – 16130ANK 2
Repeat165 – 19430ANK 3
Repeat198 – 22730ANK 4
Repeat231 – 26030ANK 5

Natural variations

Alternative sequence112 – 12110Missing in isoform Short.
VSP_000285

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 5713F335DC669E87

FASTA26929,232
        10         20         30         40         50         60 
MEPTQVAENL VPNQQPPVPD LEDPEDTRDE SPENSDTVVL SLFPCTPDAV NPEADASASS 

        70         80         90        100        110        120 
LQGSFLKHST TLTNRQRGNE VSALPATLDS LSIHQLAAQG ELSQLKDHLR KGACPACTCL 

       130        140        150        160        170        180 
SGNNLINKPD ERGFTPLIWA SAFGEIETVR FLLDWGADPH ILAKERESAL SLASMGGYTD 

       190        200        210        220        230        240 
IVRLLLDRDV DINIYDWNGG TPLLYAVRGN HVKCVEALLA RGADLTTEAD SGYTPMDLAV 

       250        260 
ALGYRKVQQV MESHILRLFQ STLGPVDPE 

« Hide

Isoform Short [UniParc].

Checksum: FC0168DC986D95C9
Show »

FASTA25928,325

References

« Hide 'large scale' references
[1]"A gene encoding a novel RFX-associated transactivator is mutated in the majority of MHC class II deficiency patients."
Masternak K., Barras E., Zufferey M., Conrad B., Corthals G., Aebersold R., Sanchez J.-C., Hochstrasser D.F., Mach B., Reith W.
Nat. Genet. 20:273-277(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Spleen.
[2]"The ankyrin repeat-containing adaptor protein tvl-1 is a novel substrate and regulator of raf-1."
Lin J.-H., Makris A., McMahon C., Bear S.E., Patriotis C., Prasad V.R., Brent R., Golemis E.A., Tsichlis P.N.
J. Biol. Chem. 274:14706-14715(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: T-cell.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
Strain: FVB/N.
Tissue: Salivary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF094761 mRNA. Translation: AAC69884.1.
AF123704 mRNA. Translation: AAD24798.1.
BC010971 mRNA. Translation: AAH10971.1.
RefSeqNP_001020760.1. NM_001025589.1.
NP_035396.1. NM_011266.2.
UniGeneMm.161167.

3D structure databases

ProteinModelPortalQ9Z205.
SMRQ9Z205. Positions 50-261.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202875. 7 interactions.

PTM databases

PhosphoSiteQ9Z205.

Proteomic databases

PRIDEQ9Z205.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000075724; ENSMUSP00000075140; ENSMUSG00000036120. [Q9Z205-1]
GeneID19727.
KEGGmmu:19727.
UCSCuc009lyt.1. mouse. [Q9Z205-1]

Organism-specific databases

CTD8625.
MGIMGI:1333865. Rfxank.

Phylogenomic databases

eggNOGCOG0666.
GeneTreeENSGT00730000110740.
HOGENOMHOG000294108.
HOVERGENHBG017718.
InParanoidQ9Z205.
KOK08062.
OMAMEPTQPA.
OrthoDBEOG7WMCJS.
PhylomeDBQ9Z205.
TreeFamTF333112.

Gene expression databases

ArrayExpressQ9Z205.
BgeeQ9Z205.
GenevestigatorQ9Z205.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017362. DNA-bd_RFXANK.
[Graphical view]
PfamPF00023. Ank. 1 hit.
PF12796. Ank_2. 1 hit.
[Graphical view]
PIRSFPIRSF038034. DNA-binding_RFXANK. 1 hit.
SMARTSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio297140.
PROQ9Z205.
SOURCESearch...

Entry information

Entry nameRFXK_MOUSE
AccessionPrimary (citable) accession number: Q9Z205
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot