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Protein

Heterogeneous nuclear ribonucleoproteins C1/C2

Gene

Hnrnpc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm6A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-4570464. SUMOylation of RNA binding proteins.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72203. Processing of Capped Intron-Containing Pre-mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoproteins C1/C2
Short name:
hnRNP C1/C2
Gene namesi
Name:Hnrnpc
Synonyms:Hnrpc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:107795. Hnrnpc.

Subcellular locationi

  • Nucleus By similarity

  • Note: Component of ribonucleosomes.By similarity

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: MGI
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • membrane Source: MGI
  • nuclear chromatin Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • pronucleus Source: MGI
  • protein complex Source: MGI
  • spliceosomal complex Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000818452 – 313Heterogeneous nuclear ribonucleoproteins C1/C2Add BLAST312

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki50Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki89Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki94Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei113PhosphoserineBy similarity1
Modified residuei115PhosphoserineBy similarity1
Modified residuei121PhosphoserineCombined sources1
Modified residuei162PhosphoserineBy similarity1
Modified residuei166PhosphoserineBy similarity1
Modified residuei176N6-acetyllysine; alternateCombined sources1
Cross-linki176Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki224Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei229PhosphoserineCombined sources1
Modified residuei231PhosphoserineCombined sources1
Modified residuei232PhosphoserineCombined sources1
Cross-linki237Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki240Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki240Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei241PhosphoserineCombined sources1
Modified residuei246PhosphoserineBy similarity1
Modified residuei247PhosphoserineBy similarity1
Modified residuei249PhosphoserineBy similarity1
Cross-linki251Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki252Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki258Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei261PhosphoserineBy similarity1
Modified residuei268PhosphoserineCombined sources1
Modified residuei306PhosphoserineCombined sources1
Modified residuei313PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on Ser-268 and Ser-306 in resting cells.By similarity
Sumoylated. Sumoylation reduces affinity for mRNA.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9Z204.
PaxDbiQ9Z204.
PeptideAtlasiQ9Z204.
PRIDEiQ9Z204.
TopDownProteomicsiQ9Z204-1. [Q9Z204-1]
Q9Z204-4. [Q9Z204-4]

PTM databases

iPTMnetiQ9Z204.
PhosphoSitePlusiQ9Z204.

Expressioni

Gene expression databases

BgeeiENSMUSG00000060373.
CleanExiMM_HNRNPC.
GenevisibleiQ9Z204. MM.

Interactioni

Subunit structurei

Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. Assembly of 3 tetramers with bound pre-mRNA gives rise to a 19S complex that interacts with HNRNPA2B1 tetramers. Component of the 40S hnRNP particle. Identified in the spliceosome C complex. Interacts with IGF2BP1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200356. 3 interactors.
DIPiDIP-59747N.
IntActiQ9Z204. 2 interactors.
MINTiMINT-1864181.
STRINGi10090.ENSMUSP00000107237.

Structurei

3D structure databases

ProteinModelPortaliQ9Z204.
SMRiQ9Z204.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 87RRMPROSITE-ProRule annotationAdd BLAST72

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili191 – 226Sequence analysisAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi155 – 161Nuclear localization signalSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi181 – 303Asp/Glu-rich (acidic)Add BLAST123

Sequence similaritiesi

Belongs to the RRM HNRPC family. RALY subfamily.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00390000006718.
HOVERGENiHBG002302.
InParanoidiQ9Z204.
KOiK12884.
OMAiPVEMKND.
OrthoDBiEOG091G0O1Q.
PhylomeDBiQ9Z204.
TreeFamiTF330974.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR017347. hnRNP_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF037992. hnRNP-C_Raly. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform C2 (identifier: Q9Z204-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASNVTNKTD PRSMNSRVFI GNLNTLVVKK SDVEAIFSKY GKIVGCSVHK
60 70 80 90 100
GFAFVQYVNE RNARAAVAGE DGRMIAGQVL DINLAAEPKV NRGKAGVKRS
110 120 130 140 150
AAEMYGSVPE HPSPSPLLSS SFDLDYDFQR DYYDRMYSYP ARVPPPPPIA
160 170 180 190 200
RAVVPSKRQR VSGNTSRRGK SGFNSKSGQR GSSSKSGKLK GDDLQAIKKE
210 220 230 240 250
LTQIKQKVDS LLESLEKIEK EQSKQADLSF SSPVEMKNEK SEEEQSSASV
260 270 280 290 300
KKDETNVKME SEAGADDSAE EGDLLDDDDN EDRGDDQLEL KDDEKEPEEG
310
EDDRDSANGE DDS
Length:313
Mass (Da):34,385
Last modified:May 1, 1999 - v1
Checksum:i4AB834051E3E301B
GO
Isoform C1 (identifier: Q9Z204-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     108-120: Missing.

Show »
Length:300
Mass (Da):33,056
Checksum:i5D602FF5656B750B
GO
Isoform 3 (identifier: Q9Z204-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     108-120: Missing.
     227-233: Missing.

Show »
Length:293
Mass (Da):32,323
Checksum:i7AC110DB06014FEC
GO
Isoform 4 (identifier: Q9Z204-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     108-120: Missing.
     227-234: Missing.

Show »
Length:292
Mass (Da):32,223
Checksum:i84A26D34AABD5806
GO
Isoform 5 (identifier: Q9Z204-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     227-233: Missing.

Show »
Length:306
Mass (Da):33,651
Checksum:i7AD8B459F9A7FF9B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46C → R in AAH04706 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005832108 – 120Missing in isoform C1, isoform 3 and isoform 4. 2 PublicationsAdd BLAST13
Alternative sequenceiVSP_019227227 – 234Missing in isoform 4. 1 Publication8
Alternative sequenceiVSP_005833227 – 233Missing in isoform 3 and isoform 5. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095257 mRNA. Translation: AAD03717.1.
AF095258 Genomic DNA. Translation: AAD19892.1.
AK011336 mRNA. Translation: BAB27553.1.
AK012633 mRNA. Translation: BAB28370.1.
AK019958 mRNA. Translation: BAB31934.1.
AK088245 mRNA. Translation: BAC40233.1.
AK088678 mRNA. Translation: BAC40499.1.
AK089061 mRNA. Translation: BAC40728.1.
AK166590 mRNA. Translation: BAE38877.1.
AK168802 mRNA. Translation: BAE40632.1.
BC004706 mRNA. Translation: AAH04706.1.
BC095922 mRNA. Translation: AAH95922.1.
CCDSiCCDS36917.1. [Q9Z204-1]
RefSeqiNP_001164452.1. NM_001170981.1. [Q9Z204-2]
NP_001164453.1. NM_001170982.1. [Q9Z204-2]
NP_001164454.1. NM_001170983.1. [Q9Z204-3]
NP_001164455.1. NM_001170984.1. [Q9Z204-4]
NP_058580.1. NM_016884.3. [Q9Z204-1]
XP_006518634.1. XM_006518571.3. [Q9Z204-1]
XP_006518635.1. XM_006518572.3. [Q9Z204-1]
XP_006518637.1. XM_006518574.2. [Q9Z204-5]
XP_006518641.1. XM_006518578.2. [Q9Z204-3]
XP_006518644.1. XM_006518581.2. [Q9Z204-4]
XP_017171338.1. XM_017315849.1. [Q9Z204-1]
XP_017171339.1. XM_017315850.1. [Q9Z204-5]
XP_017171340.1. XM_017315851.1. [Q9Z204-5]
XP_017171344.1. XM_017315855.1. [Q9Z204-2]
XP_017171345.1. XM_017315856.1. [Q9Z204-2]
XP_017171346.1. XM_017315857.1. [Q9Z204-3]
XP_017171347.1. XM_017315858.1. [Q9Z204-3]
XP_017171348.1. XM_017315859.1. [Q9Z204-4]
UniGeneiMm.427321.
Mm.473169.

Genome annotation databases

EnsembliENSMUST00000111610; ENSMUSP00000107237; ENSMUSG00000060373. [Q9Z204-1]
ENSMUST00000164655; ENSMUSP00000133052; ENSMUSG00000060373. [Q9Z204-1]
GeneIDi15381.
KEGGimmu:15381.
UCSCiuc007tob.2. mouse. [Q9Z204-1]
uc007toc.2. mouse. [Q9Z204-4]
uc007tod.2. mouse. [Q9Z204-2]
uc007toe.2. mouse. [Q9Z204-3]
uc007tof.2. mouse. [Q9Z204-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095257 mRNA. Translation: AAD03717.1.
AF095258 Genomic DNA. Translation: AAD19892.1.
AK011336 mRNA. Translation: BAB27553.1.
AK012633 mRNA. Translation: BAB28370.1.
AK019958 mRNA. Translation: BAB31934.1.
AK088245 mRNA. Translation: BAC40233.1.
AK088678 mRNA. Translation: BAC40499.1.
AK089061 mRNA. Translation: BAC40728.1.
AK166590 mRNA. Translation: BAE38877.1.
AK168802 mRNA. Translation: BAE40632.1.
BC004706 mRNA. Translation: AAH04706.1.
BC095922 mRNA. Translation: AAH95922.1.
CCDSiCCDS36917.1. [Q9Z204-1]
RefSeqiNP_001164452.1. NM_001170981.1. [Q9Z204-2]
NP_001164453.1. NM_001170982.1. [Q9Z204-2]
NP_001164454.1. NM_001170983.1. [Q9Z204-3]
NP_001164455.1. NM_001170984.1. [Q9Z204-4]
NP_058580.1. NM_016884.3. [Q9Z204-1]
XP_006518634.1. XM_006518571.3. [Q9Z204-1]
XP_006518635.1. XM_006518572.3. [Q9Z204-1]
XP_006518637.1. XM_006518574.2. [Q9Z204-5]
XP_006518641.1. XM_006518578.2. [Q9Z204-3]
XP_006518644.1. XM_006518581.2. [Q9Z204-4]
XP_017171338.1. XM_017315849.1. [Q9Z204-1]
XP_017171339.1. XM_017315850.1. [Q9Z204-5]
XP_017171340.1. XM_017315851.1. [Q9Z204-5]
XP_017171344.1. XM_017315855.1. [Q9Z204-2]
XP_017171345.1. XM_017315856.1. [Q9Z204-2]
XP_017171346.1. XM_017315857.1. [Q9Z204-3]
XP_017171347.1. XM_017315858.1. [Q9Z204-3]
XP_017171348.1. XM_017315859.1. [Q9Z204-4]
UniGeneiMm.427321.
Mm.473169.

3D structure databases

ProteinModelPortaliQ9Z204.
SMRiQ9Z204.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200356. 3 interactors.
DIPiDIP-59747N.
IntActiQ9Z204. 2 interactors.
MINTiMINT-1864181.
STRINGi10090.ENSMUSP00000107237.

PTM databases

iPTMnetiQ9Z204.
PhosphoSitePlusiQ9Z204.

Proteomic databases

EPDiQ9Z204.
PaxDbiQ9Z204.
PeptideAtlasiQ9Z204.
PRIDEiQ9Z204.
TopDownProteomicsiQ9Z204-1. [Q9Z204-1]
Q9Z204-4. [Q9Z204-4]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000111610; ENSMUSP00000107237; ENSMUSG00000060373. [Q9Z204-1]
ENSMUST00000164655; ENSMUSP00000133052; ENSMUSG00000060373. [Q9Z204-1]
GeneIDi15381.
KEGGimmu:15381.
UCSCiuc007tob.2. mouse. [Q9Z204-1]
uc007toc.2. mouse. [Q9Z204-4]
uc007tod.2. mouse. [Q9Z204-2]
uc007toe.2. mouse. [Q9Z204-3]
uc007tof.2. mouse. [Q9Z204-5]

Organism-specific databases

CTDi3183.
MGIiMGI:107795. Hnrnpc.

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00390000006718.
HOVERGENiHBG002302.
InParanoidiQ9Z204.
KOiK12884.
OMAiPVEMKND.
OrthoDBiEOG091G0O1Q.
PhylomeDBiQ9Z204.
TreeFamiTF330974.

Enzyme and pathway databases

ReactomeiR-MMU-4570464. SUMOylation of RNA binding proteins.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72203. Processing of Capped Intron-Containing Pre-mRNA.

Miscellaneous databases

ChiTaRSiHnrnpc. mouse.
PROiQ9Z204.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000060373.
CleanExiMM_HNRNPC.
GenevisibleiQ9Z204. MM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR017347. hnRNP_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF037992. hnRNP-C_Raly. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHNRPC_MOUSE
AccessioniPrimary (citable) accession number: Q9Z204
Secondary accession number(s): Q3TLB5
, Q501Q3, Q8C2G5, Q99KE2, Q9CQT3, Q9CY83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.