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Q9Z1Z6

- ILKAP_RAT

UniProt

Q9Z1Z6 - ILKAP_RAT

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Protein

Integrin-linked kinase-associated serine/threonine phosphatase 2C

Gene

Ilkap

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi152 – 1521Manganese 1By similarity
Metal bindingi152 – 1521Manganese 2By similarity
Metal bindingi153 – 1531Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi326 – 3261Manganese 2By similarity
Metal bindingi381 – 3811Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: RGD

GO - Biological processi

  1. protein dephosphorylation Source: RGD
  2. regulation of nuclear cell cycle DNA replication Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin-linked kinase-associated serine/threonine phosphatase 2C (EC:3.1.3.16)
Short name:
ILKAP
Alternative name(s):
PP2Cdelta
Gene namesi
Name:Ilkap
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 9

Organism-specific databases

RGDi620128. Ilkap.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Integrin-linked kinase-associated serine/threonine phosphatase 2CPRO_0000272273Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei210 – 2101N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9Z1Z6.
PRIDEiQ9Z1Z6.

PTM databases

PhosphoSiteiQ9Z1Z6.

Expressioni

Tissue specificityi

Widely expressed. Highest expression observed in kidney, liver and muscle.1 Publication

Inductioni

Activated in response to stress, such as the addition of ethanol to the culture medium or UV irradiation of cells. Inhibited rather than stimulated, by Magnesium.1 Publication

Gene expression databases

GenevestigatoriQ9Z1Z6.

Interactioni

Subunit structurei

Interacts with ILK.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9Z1Z6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini128 – 390263PP2C-likeAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PP2C-like domain.Curated

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000114971.
HOGENOMiHOG000233896.
HOVERGENiHBG054286.
InParanoidiQ9Z1Z6.
KOiK17500.
OrthoDBiEOG7Z95NF.
PhylomeDBiQ9Z1Z6.
TreeFamiTF313513.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z1Z6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDLFGDLPEP ERPPRPSAGK EAQEGPVLFE DLPPTSSTDS GSGGPLLFDG
60 70 80 90 100
LPPAGSGNSG SLATSGSQVV KNEGKGAKRK APEEEKNGGE ELVEKKVCKA
110 120 130 140 150
SSVIFGLKGY VAERKGEREE MQDAHVILND ITQECNPPSS LITRVSYFAV
160 170 180 190 200
FDGHGGIRAS KFAAQNLHQN LIRKFPKGDV ISVEKTVKRC LLDTFKHTDE
210 220 230 240 250
EFLKQASSQK PAWKDGSTAT CVLAVDNILY IANLGDSRAI LCRYNEESQK
260 270 280 290 300
HAALSLSKEH NPTQYEERMR IQKAGGNVRD GRVLGVLEVS RSIGDGQYKR
310 320 330 340 350
CGVTSVPDIR RCQLTPNDRF ILLACDGLFK VFTPEEAVNF ILSCLEDEKI
360 370 380 390
QTREGKPAVD ARYEAACNRL ANKAVQRGSA DNVTVMVVRI GH
Length:392
Mass (Da):42,744
Last modified:May 1, 1999 - v1
Checksum:i1468FDE0BA60D915
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131P → A in AAH62010. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF095927 mRNA. Translation: AAC97497.1.
BC062010 mRNA. Translation: AAH62010.1.
RefSeqiNP_072128.1. NM_022606.1.
UniGeneiRn.6446.

Genome annotation databases

EnsembliENSRNOT00000027295; ENSRNOP00000027295; ENSRNOG00000020115.
GeneIDi64538.
KEGGirno:64538.
UCSCiRGD:620128. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF095927 mRNA. Translation: AAC97497.1 .
BC062010 mRNA. Translation: AAH62010.1 .
RefSeqi NP_072128.1. NM_022606.1.
UniGenei Rn.6446.

3D structure databases

ProteinModelPortali Q9Z1Z6.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q9Z1Z6.

Proteomic databases

PaxDbi Q9Z1Z6.
PRIDEi Q9Z1Z6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000027295 ; ENSRNOP00000027295 ; ENSRNOG00000020115 .
GeneIDi 64538.
KEGGi rno:64538.
UCSCi RGD:620128. rat.

Organism-specific databases

CTDi 80895.
RGDi 620128. Ilkap.

Phylogenomic databases

eggNOGi COG0631.
GeneTreei ENSGT00740000114971.
HOGENOMi HOG000233896.
HOVERGENi HBG054286.
InParanoidi Q9Z1Z6.
KOi K17500.
OrthoDBi EOG7Z95NF.
PhylomeDBi Q9Z1Z6.
TreeFami TF313513.

Miscellaneous databases

NextBioi 613398.
PROi Q9Z1Z6.

Gene expression databases

Genevestigatori Q9Z1Z6.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a novel mammalian PP2C isozyme."
    Tong Y., Quirion R., Shen S.-H.
    J. Biol. Chem. 273:35282-35290(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], COFACTOR, TISSUE SPECIFICITY, INDUCTION, POSSIBLE FUNCTION.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiILKAP_RAT
AccessioniPrimary (citable) accession number: Q9Z1Z6
Secondary accession number(s): Q6P6U8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3