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Q9Z1Z6 (ILKAP_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Integrin-linked kinase-associated serine/threonine phosphatase 2C

Short name=ILKAP
EC=3.1.3.16
Alternative name(s):
PP2Cdelta
Gene names
Name:Ilkap
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length392 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation By similarity. Ref.1

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. Ref.1

Subunit structure

Interacts with ILK By similarity.

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Widely expressed. Highest expression observed in kidney, liver and muscle. Ref.1

Induction

Activated in response to stress, such as the addition of ethanol to the culture medium or UV irradiation of cells. Inhibited rather than stimulated, by Magnesium. Ref.1

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 392392Integrin-linked kinase-associated serine/threonine phosphatase 2C
PRO_0000272273

Regions

Domain128 – 390263PP2C-like

Sites

Metal binding1521Manganese 1 By similarity
Metal binding1521Manganese 2 By similarity
Metal binding1531Manganese 1; via carbonyl oxygen By similarity
Metal binding3261Manganese 2 By similarity
Metal binding3811Manganese 2 By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue2101N6-acetyllysine By similarity

Experimental info

Sequence conflict131P → A in AAH62010. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9Z1Z6 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 1468FDE0BA60D915

FASTA39242,744
        10         20         30         40         50         60 
MDLFGDLPEP ERPPRPSAGK EAQEGPVLFE DLPPTSSTDS GSGGPLLFDG LPPAGSGNSG 

        70         80         90        100        110        120 
SLATSGSQVV KNEGKGAKRK APEEEKNGGE ELVEKKVCKA SSVIFGLKGY VAERKGEREE 

       130        140        150        160        170        180 
MQDAHVILND ITQECNPPSS LITRVSYFAV FDGHGGIRAS KFAAQNLHQN LIRKFPKGDV 

       190        200        210        220        230        240 
ISVEKTVKRC LLDTFKHTDE EFLKQASSQK PAWKDGSTAT CVLAVDNILY IANLGDSRAI 

       250        260        270        280        290        300 
LCRYNEESQK HAALSLSKEH NPTQYEERMR IQKAGGNVRD GRVLGVLEVS RSIGDGQYKR 

       310        320        330        340        350        360 
CGVTSVPDIR RCQLTPNDRF ILLACDGLFK VFTPEEAVNF ILSCLEDEKI QTREGKPAVD 

       370        380        390 
ARYEAACNRL ANKAVQRGSA DNVTVMVVRI GH 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of a novel mammalian PP2C isozyme."
Tong Y., Quirion R., Shen S.-H.
J. Biol. Chem. 273:35282-35290(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], COFACTOR, TISSUE SPECIFICITY, INDUCTION, POSSIBLE FUNCTION.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF095927 mRNA. Translation: AAC97497.1.
BC062010 mRNA. Translation: AAH62010.1.
RefSeqNP_072128.1. NM_022606.1.
UniGeneRn.6446.

3D structure databases

ProteinModelPortalQ9Z1Z6.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9Z1Z6.

Proteomic databases

PaxDbQ9Z1Z6.
PRIDEQ9Z1Z6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000027295; ENSRNOP00000027295; ENSRNOG00000020115.
GeneID64538.
KEGGrno:64538.
UCSCRGD:620128. rat.

Organism-specific databases

CTD80895.
RGD620128. Ilkap.

Phylogenomic databases

eggNOGCOG0631.
GeneTreeENSGT00740000114971.
HOGENOMHOG000233896.
HOVERGENHBG054286.
InParanoidQ9Z1Z6.
KOK17500.
OrthoDBEOG7Z95NF.
PhylomeDBQ9Z1Z6.
TreeFamTF313513.

Gene expression databases

GenevestigatorQ9Z1Z6.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio613398.
PROQ9Z1Z6.

Entry information

Entry nameILKAP_RAT
AccessionPrimary (citable) accession number: Q9Z1Z6
Secondary accession number(s): Q6P6U8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families