Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9Z1Z6

- ILKAP_RAT

UniProt

Q9Z1Z6 - ILKAP_RAT

Protein

Integrin-linked kinase-associated serine/threonine phosphatase 2C

Gene

Ilkap

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation By similarity.By similarity

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi152 – 1521Manganese 1By similarity
    Metal bindingi152 – 1521Manganese 2By similarity
    Metal bindingi153 – 1531Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi326 – 3261Manganese 2By similarity
    Metal bindingi381 – 3811Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein serine/threonine phosphatase activity Source: RGD

    GO - Biological processi

    1. protein dephosphorylation Source: RGD
    2. regulation of nuclear cell cycle DNA replication Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Integrin-linked kinase-associated serine/threonine phosphatase 2C (EC:3.1.3.16)
    Short name:
    ILKAP
    Alternative name(s):
    PP2Cdelta
    Gene namesi
    Name:Ilkap
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 9

    Organism-specific databases

    RGDi620128. Ilkap.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 392392Integrin-linked kinase-associated serine/threonine phosphatase 2CPRO_0000272273Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei210 – 2101N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ9Z1Z6.
    PRIDEiQ9Z1Z6.

    PTM databases

    PhosphoSiteiQ9Z1Z6.

    Expressioni

    Tissue specificityi

    Widely expressed. Highest expression observed in kidney, liver and muscle.1 Publication

    Inductioni

    Activated in response to stress, such as the addition of ethanol to the culture medium or UV irradiation of cells. Inhibited rather than stimulated, by Magnesium.1 Publication

    Gene expression databases

    GenevestigatoriQ9Z1Z6.

    Interactioni

    Subunit structurei

    Interacts with ILK.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z1Z6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini128 – 390263PP2C-likeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Phylogenomic databases

    eggNOGiCOG0631.
    GeneTreeiENSGT00740000114971.
    HOGENOMiHOG000233896.
    HOVERGENiHBG054286.
    InParanoidiQ9Z1Z6.
    KOiK17500.
    OrthoDBiEOG7Z95NF.
    PhylomeDBiQ9Z1Z6.
    TreeFamiTF313513.

    Family and domain databases

    Gene3Di3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 1 hit.
    PROSITEiPS01032. PP2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z1Z6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDLFGDLPEP ERPPRPSAGK EAQEGPVLFE DLPPTSSTDS GSGGPLLFDG    50
    LPPAGSGNSG SLATSGSQVV KNEGKGAKRK APEEEKNGGE ELVEKKVCKA 100
    SSVIFGLKGY VAERKGEREE MQDAHVILND ITQECNPPSS LITRVSYFAV 150
    FDGHGGIRAS KFAAQNLHQN LIRKFPKGDV ISVEKTVKRC LLDTFKHTDE 200
    EFLKQASSQK PAWKDGSTAT CVLAVDNILY IANLGDSRAI LCRYNEESQK 250
    HAALSLSKEH NPTQYEERMR IQKAGGNVRD GRVLGVLEVS RSIGDGQYKR 300
    CGVTSVPDIR RCQLTPNDRF ILLACDGLFK VFTPEEAVNF ILSCLEDEKI 350
    QTREGKPAVD ARYEAACNRL ANKAVQRGSA DNVTVMVVRI GH 392
    Length:392
    Mass (Da):42,744
    Last modified:May 1, 1999 - v1
    Checksum:i1468FDE0BA60D915
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131P → A in AAH62010. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF095927 mRNA. Translation: AAC97497.1.
    BC062010 mRNA. Translation: AAH62010.1.
    RefSeqiNP_072128.1. NM_022606.1.
    UniGeneiRn.6446.

    Genome annotation databases

    EnsembliENSRNOT00000027295; ENSRNOP00000027295; ENSRNOG00000020115.
    GeneIDi64538.
    KEGGirno:64538.
    UCSCiRGD:620128. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF095927 mRNA. Translation: AAC97497.1 .
    BC062010 mRNA. Translation: AAH62010.1 .
    RefSeqi NP_072128.1. NM_022606.1.
    UniGenei Rn.6446.

    3D structure databases

    ProteinModelPortali Q9Z1Z6.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9Z1Z6.

    Proteomic databases

    PaxDbi Q9Z1Z6.
    PRIDEi Q9Z1Z6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000027295 ; ENSRNOP00000027295 ; ENSRNOG00000020115 .
    GeneIDi 64538.
    KEGGi rno:64538.
    UCSCi RGD:620128. rat.

    Organism-specific databases

    CTDi 80895.
    RGDi 620128. Ilkap.

    Phylogenomic databases

    eggNOGi COG0631.
    GeneTreei ENSGT00740000114971.
    HOGENOMi HOG000233896.
    HOVERGENi HBG054286.
    InParanoidi Q9Z1Z6.
    KOi K17500.
    OrthoDBi EOG7Z95NF.
    PhylomeDBi Q9Z1Z6.
    TreeFami TF313513.

    Miscellaneous databases

    NextBioi 613398.
    PROi Q9Z1Z6.

    Gene expression databases

    Genevestigatori Q9Z1Z6.

    Family and domain databases

    Gene3Di 3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 1 hit.
    PROSITEi PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a novel mammalian PP2C isozyme."
      Tong Y., Quirion R., Shen S.-H.
      J. Biol. Chem. 273:35282-35290(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], COFACTOR, TISSUE SPECIFICITY, INDUCTION, POSSIBLE FUNCTION.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.

    Entry informationi

    Entry nameiILKAP_RAT
    AccessioniPrimary (citable) accession number: Q9Z1Z6
    Secondary accession number(s): Q6P6U8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3