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Protein

Epsin-2

Gene

Epn2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the formation of clathrin-coated invaginations and endocytosis.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei8Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei11Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei25Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei30Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei63Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei73Phosphatidylinositol lipid headgroupBy similarity1

GO - Molecular functioni

GO - Biological processi

  • endocytosis Source: UniProtKB-KW
  • regulation of endocytosis Source: RGD
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Epsin-2
Alternative name(s):
EPS-15-interacting protein 2
Gene namesi
Name:Epn2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619773. Epn2.

Subcellular locationi

  • Cytoplasm By similarity

  • Note: In punctate structures throughout the cell and particularly concentrated in the region of the Golgi complex.By similarity

GO - Cellular componenti

  • clathrin coat of endocytic vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745181 – 583Epsin-2Add BLAST583

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei170Omega-N-methylarginineBy similarity1
Modified residuei173PhosphoserineBy similarity1
Modified residuei192PhosphoserineCombined sources1
Modified residuei195PhosphoserineCombined sources1
Modified residuei431PhosphoserineCombined sources1
Modified residuei453PhosphothreonineCombined sources1
Modified residuei514PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated.1 Publication

Keywords - PTMi

Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ9Z1Z3.

PTM databases

iPTMnetiQ9Z1Z3.

Expressioni

Tissue specificityi

Highly expressed in brain. Detected at lower levels in lung, liver, muscle and testis.1 Publication

Interactioni

Subunit structurei

Binds AP-2 and clathrin (By similarity). Interacts with ITSN1 (By similarity). Interacts with UBQLN2 (By similarity). Binds EPS15.By similarity1 Publication

Protein-protein interaction databases

BioGridi248836. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1Z3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 144ENTHPROSITE-ProRule annotationAdd BLAST133
Domaini218 – 237UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini243 – 262UIM 2PROSITE-ProRule annotationAdd BLAST20
Repeati301 – 30313
Repeati313 – 31523
Repeati326 – 32833
Repeati340 – 34243
Repeati358 – 36053
Repeati375 – 37763
Repeati482 – 48413
Repeati496 – 49823
Repeati579 – 58133

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni301 – 3776 X 3 AA repeats of [DE]-P-WAdd BLAST77
Regioni482 – 5813 X 3 AA repeats of N-P-FAdd BLAST100

Domaini

The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP-2 and clathrin binding.

Sequence similaritiesi

Belongs to the epsin family.Curated
Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation
Contains 2 UIM (ubiquitin-interacting motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000008298.
HOVERGENiHBG006690.
InParanoidiQ9Z1Z3.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR027319. Epsin-2_metazoa.
IPR003903. UIM_dom.
[Graphical view]
PANTHERiPTHR12276:SF50. PTHR12276:SF50. 1 hit.
PfamiPF01417. ENTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
SM00726. UIM. 2 hits.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
PS50330. UIM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z1Z3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSSIRRQM KNIVNSYSEA EIKVREATSN DPWGPSSSLM TEIADLTYNV
60 70 80 90 100
VRFSEIMSMV WKRLNDHGKN WRHVYKALTL LDYLIKTGSE RVAQQCRENI
110 120 130 140 150
FAIQTLKDFQ YIDRDGKDQG INVREKSKQL VALLKDEERL KVERVQALKT
160 170 180 190 200
KERMAQVATG VGSNQITFGR GSSQPNLSIS HSEQEYGKAG GSPASYHGST
210 220 230 240 250
SPRVSSELEQ ARPQTSGEEE LQLQLALAMS REVAEQEERL RRGDDLRLQM
260 270 280 290 300
ALEESRRDTV KVPKKKEVKA CCKPGSHSQQ TTLLDLMDAL PSSGPVAQKT
310 320 330 340 350
EPWSTGTPAN QTNPWGGTVA PANISDPWPS FGTKPAASVD PWGVPTTASI
360 370 380 390 400
QSVPKNSDPW AASQQPASDA GKTADAWGAA KPSPASGSFE LFSNFNGTVK
410 420 430 440 450
DDFSEFDNLR TSKKPAESGA SVPPQDSRTT SPDLFESQSL TSASSKPSSA
460 470 480 490 500
RKTPESFLGP NAALVNLDSL VTKPAPPAQS LNPFLAPGAA APAPVNPFQV
510 520 530 540 550
NQPQPLTLNQ LRGSPVLGSS ASFGSGPGVE TVAPMPSVAP HSALGATGSS
560 570 580
LTPLGPTAMN MVGSMGIPPS AAQPAGTTNP FLL
Length:583
Mass (Da):62,349
Last modified:May 1, 1999 - v1
Checksum:i2C2982B72C8FDC79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF096269 mRNA. Translation: AAC79495.1.
UniGeneiRn.44273.

Genome annotation databases

UCSCiRGD:619773. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF096269 mRNA. Translation: AAC79495.1.
UniGeneiRn.44273.

3D structure databases

ProteinModelPortaliQ9Z1Z3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248836. 2 interactors.

PTM databases

iPTMnetiQ9Z1Z3.

Proteomic databases

PRIDEiQ9Z1Z3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:619773. rat.

Organism-specific databases

RGDi619773. Epn2.

Phylogenomic databases

HOGENOMiHOG000008298.
HOVERGENiHBG006690.
InParanoidiQ9Z1Z3.

Miscellaneous databases

PROiQ9Z1Z3.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR027319. Epsin-2_metazoa.
IPR003903. UIM_dom.
[Graphical view]
PANTHERiPTHR12276:SF50. PTHR12276:SF50. 1 hit.
PfamiPF01417. ENTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
SM00726. UIM. 2 hits.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
PS50330. UIM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEPN2_RAT
AccessioniPrimary (citable) accession number: Q9Z1Z3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.