Q9Z1Z0 (USO1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
General vesicular transport factor p115

Alternative name(s):
Protein USO1 homolog
Transcytosis-associated protein
Short name=TAP
Vesicle-docking protein

Gene names

Name:	Uso1
Synonyms:	Vdp

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	959 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity By similarity.
Subunit structure	Homodimer. Dimerizes by parallel association of the tails, resulting in an elongated structure with two globular head domains side by side, and a long rod-like tail structure. Interacts with MIF By similarity.
Subcellular location	Cytoplasm › cytosol By similarity. Golgi apparatus membrane; Peripheral membrane protein By similarity. Note: Recycles between the cytosol and the Golgi apparatus during interphase By similarity.
Domain	Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
Post-translational modification	Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promostes dissociation By similarity. Ref.4
Sequence similarities	Belongs to the VDP/USO1/EDE1 family. Contains 10 ARM repeats.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intracellular protein transport Inferred from direct assay. Source: MGI vesicle fusion with Golgi apparatus Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from direct assay. Source: MGI microsome Inferred from direct assay. Source: MGI perinuclear region of cytoplasm Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: MGI protein transporter activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Z1Z0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Z1Z0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     764-825: Missing.

Isoform 3 (identifier: Q9Z1Z0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     485-496: GSKIQTRVGLLM → VRATGLLRGERQ
     497-959: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q9Z1Z0-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-606: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 959	959	General vesicular transport factor p115		PRO_0000065775
Regions
	Repeat	48 – 91	44	ARM 1
	Repeat	112 – 151	40	ARM 2
	Repeat	154 – 194	41	ARM 3
	Repeat	196 – 241	46	ARM 4
	Repeat	242 – 290	49	ARM 5
	Repeat	292 – 341	50	ARM 6
	Repeat	342 – 393	52	ARM 7
	Repeat	394 – 448	55	ARM 8
	Repeat	450 – 501	52	ARM 9
	Repeat	503 – 548	46	ARM 10
	Region	1 – 637	637	Globular head
	Coiled coil	638 – 930	293	Potential
	Compositional bias	935 – 959	25	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	202	1	N6-acetyllysine By similarity
	Modified residue	940	1	Phosphoserine Ref.4
Natural variations
	Alternative sequence	1 – 606	606	Missing in isoform 4.		VSP_016978
	Alternative sequence	485 – 496	12	GSKIQ…VGLLM → VRATGLLRGERQ in isoform 3.		VSP_016979
	Alternative sequence	497 – 959	463	Missing in isoform 3.		VSP_016980
	Alternative sequence	764 – 825	62	Missing in isoform 2.		VSP_016981
Experimental info
	Sequence conflict	225	1	G → R in AAC72967. Ref.3
	Sequence conflict	291	1	V → G in AAC72967. Ref.3
	Sequence conflict	377 – 378	2	QP → HA in AAC72967. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 10, 2006. Version 2. Checksum: 2C05DC35E899D81C	FASTA	959	106,983
	10 20 30 40 50 60 MNFLRGVMGG QSAGPQHTEA ETIQKLCDRV ASSTLLDDRR NAVRALKSLS KKYRLEVGIQ 70 80 90 100 110 120 AMEHLIHVLQ TDRSDSEIIA YALDTLYNII SNDEEEELEE NSTRQSEDLG SQFTEIFIKQ 130 140 150 160 170 180 PENVTLLLSL LEEFDFHVRW PGVRLLTSLL KQLGPPVQQI ILVSPMGVSR LMDLLADSRE 190 200 210 220 230 240 IIRNDGVLLL QALTRSNGAI QKIVAFENAF ERLLDIITEE GNSDGGIVVE DCLILLQNLL 250 260 270 280 290 300 KTNNSNQNFF KEGSYIQRMK PWFEVGEENS GWSAQKVTNL HLMLQLVRVL VSPTNPPGAT 310 320 330 340 350 360 SSCQKAMFQC GLLQQLCTIL MATGIPADIL TETINTVSEV IRGCQVNQDY FASVNAPSNP 370 380 390 400 410 420 PRPAIVVLLM SMVNERQPFV LRCAVLYCFQ CFLYKNEKGQ GEIVATLLPS TIDATGNSVS 430 440 450 460 470 480 AGQLLCGGLF STDSLSNWCA AVALAHALQG NATQKEQLLR VQLATSIGNP PVSLLQQCTN 490 500 510 520 530 540 ILSQGSKIQT RVGLLMLLCT WLSNCPIAVT HFLHNSANVP FLTGQIAENL GEEEQLVQGL 550 560 570 580 590 600 CALLLGISIY FNDNSLENYT KEKLKQLIEK RIGKENFIEK LGFISKHELY SRASQKPQPN 610 620 630 640 650 660 FPSPEYMIFD HEFTKLVKEL EGVITKAIYK SSEEDKKEEE VKKTLEQHDN IVTHYKNMIR 670 680 690 700 710 720 EQDLQLEELK QQVSTLKCQN EQLQTAVTQQ ASQIQQHKDQ YNLLKVQLGK DNHHQGSHGD 730 740 750 760 770 780 GAQVNGIQPE EISRLREEIE ELKSQQALLQ GQLAEKDSLI ENLKSSQASG MSEQASATCP 790 800 810 820 830 840 PRDPEQVAEL KQELTALKSQ LCSQSLEITR LQTENCELLQ RAETLAKSVP VEGESEHVSA 850 860 870 880 890 900 AKTTDVEGRL SALLQETKEL KNEIKALSEE RTAIQKQLDS SNSTIAILQT EKDKLDLEVT 910 920 930 940 950 DSKKEQDDLL VLLADQDQKI LSLKSKLKDL GHPVEEEDES GDQEDDDDEI DDGDKDQDI
	Isoform 2 [UniParc]. Checksum: 676131AB4D52BF3B	FASTA	897	100,195
	10 20 30 40 50 60 MNFLRGVMGG QSAGPQHTEA ETIQKLCDRV ASSTLLDDRR NAVRALKSLS KKYRLEVGIQ 70 80 90 100 110 120 AMEHLIHVLQ TDRSDSEIIA YALDTLYNII SNDEEEELEE NSTRQSEDLG SQFTEIFIKQ 130 140 150 160 170 180 PENVTLLLSL LEEFDFHVRW PGVRLLTSLL KQLGPPVQQI ILVSPMGVSR LMDLLADSRE 190 200 210 220 230 240 IIRNDGVLLL QALTRSNGAI QKIVAFENAF ERLLDIITEE GNSDGGIVVE DCLILLQNLL 250 260 270 280 290 300 KTNNSNQNFF KEGSYIQRMK PWFEVGEENS GWSAQKVTNL HLMLQLVRVL VSPTNPPGAT 310 320 330 340 350 360 SSCQKAMFQC GLLQQLCTIL MATGIPADIL TETINTVSEV IRGCQVNQDY FASVNAPSNP 370 380 390 400 410 420 PRPAIVVLLM SMVNERQPFV LRCAVLYCFQ CFLYKNEKGQ GEIVATLLPS TIDATGNSVS 430 440 450 460 470 480 AGQLLCGGLF STDSLSNWCA AVALAHALQG NATQKEQLLR VQLATSIGNP PVSLLQQCTN 490 500 510 520 530 540 ILSQGSKIQT RVGLLMLLCT WLSNCPIAVT HFLHNSANVP FLTGQIAENL GEEEQLVQGL 550 560 570 580 590 600 CALLLGISIY FNDNSLENYT KEKLKQLIEK RIGKENFIEK LGFISKHELY SRASQKPQPN 610 620 630 640 650 660 FPSPEYMIFD HEFTKLVKEL EGVITKAIYK SSEEDKKEEE VKKTLEQHDN IVTHYKNMIR 670 680 690 700 710 720 EQDLQLEELK QQVSTLKCQN EQLQTAVTQQ ASQIQQHKDQ YNLLKVQLGK DNHHQGSHGD 730 740 750 760 770 780 GAQVNGIQPE EISRLREEIE ELKSQQALLQ GQLAEKDSLI ENLAKSVPVE GESEHVSAAK 790 800 810 820 830 840 TTDVEGRLSA LLQETKELKN EIKALSEERT AIQKQLDSSN STIAILQTEK DKLDLEVTDS 850 860 870 880 890 KKEQDDLLVL LADQDQKILS LKSKLKDLGH PVEEEDESGD QEDDDDEIDD GDKDQDI
	Isoform 3 [UniParc]. Checksum: 0987BCE5E38C6EEA	FASTA	496	54,732
	10 20 30 40 50 60 MNFLRGVMGG QSAGPQHTEA ETIQKLCDRV ASSTLLDDRR NAVRALKSLS KKYRLEVGIQ 70 80 90 100 110 120 AMEHLIHVLQ TDRSDSEIIA YALDTLYNII SNDEEEELEE NSTRQSEDLG SQFTEIFIKQ 130 140 150 160 170 180 PENVTLLLSL LEEFDFHVRW PGVRLLTSLL KQLGPPVQQI ILVSPMGVSR LMDLLADSRE 190 200 210 220 230 240 IIRNDGVLLL QALTRSNGAI QKIVAFENAF ERLLDIITEE GNSDGGIVVE DCLILLQNLL 250 260 270 280 290 300 KTNNSNQNFF KEGSYIQRMK PWFEVGEENS GWSAQKVTNL HLMLQLVRVL VSPTNPPGAT 310 320 330 340 350 360 SSCQKAMFQC GLLQQLCTIL MATGIPADIL TETINTVSEV IRGCQVNQDY FASVNAPSNP 370 380 390 400 410 420 PRPAIVVLLM SMVNERQPFV LRCAVLYCFQ CFLYKNEKGQ GEIVATLLPS TIDATGNSVS 430 440 450 460 470 480 AGQLLCGGLF STDSLSNWCA AVALAHALQG NATQKEQLLR VQLATSIGNP PVSLLQQCTN 490 ILSQVRATGL LRGERQ
	Isoform 4 [UniParc]. Checksum: 3214EC66D1C50555	FASTA	353	39,816
	10 20 30 40 50 60 MIFDHEFTKL VKELEGVITK AIYKSSEEDK KEEEVKKTLE QHDNIVTHYK NMIREQDLQL 70 80 90 100 110 120 EELKQQVSTL KCQNEQLQTA VTQQASQIQQ HKDQYNLLKV QLGKDNHHQG SHGDGAQVNG 130 140 150 160 170 180 IQPEEISRLR EEIEELKSQQ ALLQGQLAEK DSLIENLKSS QASGMSEQAS ATCPPRDPEQ 190 200 210 220 230 240 VAELKQELTA LKSQLCSQSL EITRLQTENC ELLQRAETLA KSVPVEGESE HVSAAKTTDV 250 260 270 280 290 300 EGRLSALLQE TKELKNEIKA LSEERTAIQK QLDSSNSTIA ILQTEKDKLD LEVTDSKKEQ 310 320 330 340 350 DDLLVLLADQ DQKILSLKSK LKDLGHPVEE EDESGDQEDD DDEIDDGDKD QDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4). Strain: C57BL/6J and NOD. Tissue: Liver, Lung and Spleen.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Eye and Mammary tumor.
[3]	"Identification of mouse TAP (transcytosis associated protein/p115)." Han S.J. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-959 (ISOFORM 1). Strain: 129. Tissue: Thymus.
[4]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 19367708] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-940, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK144638 mRNA. Translation: BAE25982.1. AK156070 mRNA. Translation: BAE33571.1. AK168433 mRNA. Translation: BAE40340.1. AK172425 mRNA. Translation: BAE43001.1. BC005548 mRNA. Translation: AAH05548.1. BC016069 mRNA. Translation: AAH16069.1. AF096868 mRNA. Translation: AAC72967.1.
IPI	IPI00128071. IPI00659170. IPI00663122. IPI00757596.
RefSeq	NP_062363.1. NM_019490.1.
UniGene	Mm.15868.
3D structure databases
ProteinModelPortal	Q9Z1Z0.
SMR	Q9Z1Z0. Positions 17-629.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1864334.
STRING	Q9Z1Z0.
PTM databases
PhosphoSite	Q9Z1Z0.
Proteomic databases
PRIDE	Q9Z1Z0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000031355; ENSMUSP00000031355; ENSMUSG00000029407. ENSMUST00000097035; ENSMUSP00000094800; ENSMUSG00000029407.
GeneID	56041.
KEGG	mmu:56041.
UCSC	uc008ycl.1. mouse. uc008ycm.1. mouse. uc008ycn.1. mouse. uc012dyl.1. mouse.
Organism-specific databases
CTD	8615.
MGI	MGI:1929095. Uso1.
Phylogenomic databases
eggNOG	roNOG06411.
GeneTree	ENSGT00390000017018.
HOVERGEN	HBG018067.
InParanoid	Q9Z1Z0.
OMA	HKDQYNL.
OrthoDB	EOG4PC9RK.
PhylomeDB	Q9Z1Z0.
Gene expression databases
ArrayExpress	Q9Z1Z0.
Bgee	Q9Z1Z0.
CleanEx	MM_USO1.
Genevestigator	Q9Z1Z0.
GermOnline	ENSMUSG00000029407. Mus musculus.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR000225. Armadillo. IPR006955. Uso1_p115_C. IPR024095. Vesicle_P115-like. IPR006953. Vesicle_Uso1_P115_head. [Graphical view]
PANTHER	PTHR10013. PTHR10013. 1 hit.
Pfam	PF04871. Uso1_p115_C. 1 hit. PF04869. Uso1_p115_head. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 2 hits.
PROSITE	PS50176. ARM_REPEAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	311804.
SOURCE	Search...

Entry information

Entry name

USO1_MOUSE

Accession

Primary (citable) accession number: Q9Z1Z0
Secondary accession number(s): Q3T9L9 , Q3TH58, Q3U1C7, Q3UMW6, Q91WE7, Q99JZ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	January 10, 2006
Last modified:	September 21, 2011
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program