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Protein

General vesicular transport factor p115

Gene

Uso1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.By similarity

GO - Molecular functioni

  • poly(A) RNA binding Source: MGI
  • protein transporter activity Source: MGI

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • Golgi organization Source: MGI
  • intracellular protein transport Source: MGI
  • transcytosis Source: Ensembl
  • vesicle fusion with Golgi apparatus Source: InterPro
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-6807878. COPI-mediated anterograde transport.

Names & Taxonomyi

Protein namesi
Recommended name:
General vesicular transport factor p115
Alternative name(s):
Protein USO1 homolog
Transcytosis-associated protein
Short name:
TAP
Vesicle-docking protein
Gene namesi
Name:Uso1
Synonyms:Vdp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1929095. Uso1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MGI
  • Golgi apparatus Source: MGI
  • Golgi membrane Source: UniProtKB-SubCell
  • membrane Source: MGI
  • nucleolus Source: MGI
  • perinuclear region of cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 959959General vesicular transport factor p115PRO_0000065775Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501PhosphoserineBy similarity
Modified residuei202 – 2021N6-acetyllysineBy similarity
Modified residuei940 – 9401PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promostes dissociation (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Z1Z0.
MaxQBiQ9Z1Z0.
PaxDbiQ9Z1Z0.
PRIDEiQ9Z1Z0.

PTM databases

iPTMnetiQ9Z1Z0.
PhosphoSiteiQ9Z1Z0.
SwissPalmiQ9Z1Z0.

Expressioni

Gene expression databases

BgeeiQ9Z1Z0.
CleanExiMM_USO1.
ExpressionAtlasiQ9Z1Z0. baseline and differential.
GenevisibleiQ9Z1Z0. MM.

Interactioni

Subunit structurei

Homodimer. Dimerizes by parallel association of the tails, resulting in an elongated structure with two globular head domains side by side, and a long rod-like tail structure. Interacts with MIF (By similarity).By similarity

Protein-protein interaction databases

BioGridi207788. 107 interactions.
IntActiQ9Z1Z0. 112 interactions.
MINTiMINT-1864334.
STRINGi10090.ENSMUSP00000031355.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1Z0.
SMRiQ9Z1Z0. Positions 17-629.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati20 – 6041ARM 1Add
BLAST
Repeati61 – 12161ARM 2Add
BLAST
Repeati123 – 16341ARM 3Add
BLAST
Repeati166 – 20742ARM 4Add
BLAST
Repeati208 – 25346ARM 5Add
BLAST
Repeati255 – 31056ARM 6Add
BLAST
Repeati311 – 35444ARM 7Add
BLAST
Repeati363 – 40846ARM 8Add
BLAST
Repeati420 – 45940ARM 9Add
BLAST
Repeati473 – 51341ARM 10Add
BLAST
Repeati518 – 57154ARM 11Add
BLAST
Repeati573 – 63058ARM 12Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 637637Globular headAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili638 – 930293Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi935 – 95925Asp/Glu-rich (acidic)Add
BLAST

Domaini

Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.

Sequence similaritiesi

Belongs to the VDP/USO1/EDE1 family.Curated
Contains 12 ARM repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0946. Eukaryota.
ENOG410XRCG. LUCA.
GeneTreeiENSGT00390000017018.
HOVERGENiHBG018067.
InParanoidiQ9Z1Z0.
OMAiYQVNQDY.
OrthoDBiEOG7K0ZBS.
PhylomeDBiQ9Z1Z0.
TreeFamiTF106157.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR006955. Uso1_p115_C.
IPR024095. Vesicle_P115-like.
IPR006953. Vesicle_Uso1_P115_head.
[Graphical view]
PANTHERiPTHR10013. PTHR10013. 1 hit.
PfamiPF04871. Uso1_p115_C. 1 hit.
PF04869. Uso1_p115_head. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z1Z0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNFLRGVMGG QSAGPQHTEA ETIQKLCDRV ASSTLLDDRR NAVRALKSLS
60 70 80 90 100
KKYRLEVGIQ AMEHLIHVLQ TDRSDSEIIA YALDTLYNII SNDEEEELEE
110 120 130 140 150
NSTRQSEDLG SQFTEIFIKQ PENVTLLLSL LEEFDFHVRW PGVRLLTSLL
160 170 180 190 200
KQLGPPVQQI ILVSPMGVSR LMDLLADSRE IIRNDGVLLL QALTRSNGAI
210 220 230 240 250
QKIVAFENAF ERLLDIITEE GNSDGGIVVE DCLILLQNLL KTNNSNQNFF
260 270 280 290 300
KEGSYIQRMK PWFEVGEENS GWSAQKVTNL HLMLQLVRVL VSPTNPPGAT
310 320 330 340 350
SSCQKAMFQC GLLQQLCTIL MATGIPADIL TETINTVSEV IRGCQVNQDY
360 370 380 390 400
FASVNAPSNP PRPAIVVLLM SMVNERQPFV LRCAVLYCFQ CFLYKNEKGQ
410 420 430 440 450
GEIVATLLPS TIDATGNSVS AGQLLCGGLF STDSLSNWCA AVALAHALQG
460 470 480 490 500
NATQKEQLLR VQLATSIGNP PVSLLQQCTN ILSQGSKIQT RVGLLMLLCT
510 520 530 540 550
WLSNCPIAVT HFLHNSANVP FLTGQIAENL GEEEQLVQGL CALLLGISIY
560 570 580 590 600
FNDNSLENYT KEKLKQLIEK RIGKENFIEK LGFISKHELY SRASQKPQPN
610 620 630 640 650
FPSPEYMIFD HEFTKLVKEL EGVITKAIYK SSEEDKKEEE VKKTLEQHDN
660 670 680 690 700
IVTHYKNMIR EQDLQLEELK QQVSTLKCQN EQLQTAVTQQ ASQIQQHKDQ
710 720 730 740 750
YNLLKVQLGK DNHHQGSHGD GAQVNGIQPE EISRLREEIE ELKSQQALLQ
760 770 780 790 800
GQLAEKDSLI ENLKSSQASG MSEQASATCP PRDPEQVAEL KQELTALKSQ
810 820 830 840 850
LCSQSLEITR LQTENCELLQ RAETLAKSVP VEGESEHVSA AKTTDVEGRL
860 870 880 890 900
SALLQETKEL KNEIKALSEE RTAIQKQLDS SNSTIAILQT EKDKLDLEVT
910 920 930 940 950
DSKKEQDDLL VLLADQDQKI LSLKSKLKDL GHPVEEEDES GDQEDDDDEI

DDGDKDQDI
Length:959
Mass (Da):106,983
Last modified:January 10, 2006 - v2
Checksum:i2C05DC35E899D81C
GO
Isoform 2 (identifier: Q9Z1Z0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     764-825: Missing.

Show »
Length:897
Mass (Da):100,195
Checksum:i676131AB4D52BF3B
GO
Isoform 3 (identifier: Q9Z1Z0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     485-496: GSKIQTRVGLLM → VRATGLLRGERQ
     497-959: Missing.

Note: No experimental confirmation available.
Show »
Length:496
Mass (Da):54,732
Checksum:i0987BCE5E38C6EEA
GO
Isoform 4 (identifier: Q9Z1Z0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-606: Missing.

Note: No experimental confirmation available.
Show »
Length:353
Mass (Da):39,816
Checksum:i3214EC66D1C50555
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti225 – 2251G → R in AAC72967 (Ref. 3) Curated
Sequence conflicti291 – 2911V → G in AAC72967 (Ref. 3) Curated
Sequence conflicti377 – 3782QP → HA in AAC72967 (Ref. 3) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 606606Missing in isoform 4. 1 PublicationVSP_016978Add
BLAST
Alternative sequencei485 – 49612GSKIQ…VGLLM → VRATGLLRGERQ in isoform 3. 1 PublicationVSP_016979Add
BLAST
Alternative sequencei497 – 959463Missing in isoform 3. 1 PublicationVSP_016980Add
BLAST
Alternative sequencei764 – 82562Missing in isoform 2. 1 PublicationVSP_016981Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144638 mRNA. Translation: BAE25982.1.
AK156070 mRNA. Translation: BAE33571.1.
AK168433 mRNA. Translation: BAE40340.1.
AK172425 mRNA. Translation: BAE43001.1.
BC005548 mRNA. Translation: AAH05548.1.
BC016069 mRNA. Translation: AAH16069.1.
AF096868 mRNA. Translation: AAC72967.1.
CCDSiCCDS39150.1. [Q9Z1Z0-1]
RefSeqiNP_062363.1. NM_019490.1. [Q9Z1Z0-1]
UniGeneiMm.15868.

Genome annotation databases

EnsembliENSMUST00000031355; ENSMUSP00000031355; ENSMUSG00000029407. [Q9Z1Z0-1]
ENSMUST00000202155; ENSMUSP00000144592; ENSMUSG00000029407. [Q9Z1Z0-2]
GeneIDi56041.
KEGGimmu:56041.
UCSCiuc008ycl.1. mouse. [Q9Z1Z0-3]
uc008ycm.1. mouse. [Q9Z1Z0-1]
uc008ycn.1. mouse. [Q9Z1Z0-4]
uc012dyl.1. mouse. [Q9Z1Z0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144638 mRNA. Translation: BAE25982.1.
AK156070 mRNA. Translation: BAE33571.1.
AK168433 mRNA. Translation: BAE40340.1.
AK172425 mRNA. Translation: BAE43001.1.
BC005548 mRNA. Translation: AAH05548.1.
BC016069 mRNA. Translation: AAH16069.1.
AF096868 mRNA. Translation: AAC72967.1.
CCDSiCCDS39150.1. [Q9Z1Z0-1]
RefSeqiNP_062363.1. NM_019490.1. [Q9Z1Z0-1]
UniGeneiMm.15868.

3D structure databases

ProteinModelPortaliQ9Z1Z0.
SMRiQ9Z1Z0. Positions 17-629.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207788. 107 interactions.
IntActiQ9Z1Z0. 112 interactions.
MINTiMINT-1864334.
STRINGi10090.ENSMUSP00000031355.

PTM databases

iPTMnetiQ9Z1Z0.
PhosphoSiteiQ9Z1Z0.
SwissPalmiQ9Z1Z0.

Proteomic databases

EPDiQ9Z1Z0.
MaxQBiQ9Z1Z0.
PaxDbiQ9Z1Z0.
PRIDEiQ9Z1Z0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031355; ENSMUSP00000031355; ENSMUSG00000029407. [Q9Z1Z0-1]
ENSMUST00000202155; ENSMUSP00000144592; ENSMUSG00000029407. [Q9Z1Z0-2]
GeneIDi56041.
KEGGimmu:56041.
UCSCiuc008ycl.1. mouse. [Q9Z1Z0-3]
uc008ycm.1. mouse. [Q9Z1Z0-1]
uc008ycn.1. mouse. [Q9Z1Z0-4]
uc012dyl.1. mouse. [Q9Z1Z0-2]

Organism-specific databases

CTDi8615.
MGIiMGI:1929095. Uso1.

Phylogenomic databases

eggNOGiKOG0946. Eukaryota.
ENOG410XRCG. LUCA.
GeneTreeiENSGT00390000017018.
HOVERGENiHBG018067.
InParanoidiQ9Z1Z0.
OMAiYQVNQDY.
OrthoDBiEOG7K0ZBS.
PhylomeDBiQ9Z1Z0.
TreeFamiTF106157.

Enzyme and pathway databases

ReactomeiR-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-6807878. COPI-mediated anterograde transport.

Miscellaneous databases

ChiTaRSiUso1. mouse.
PROiQ9Z1Z0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z1Z0.
CleanExiMM_USO1.
ExpressionAtlasiQ9Z1Z0. baseline and differential.
GenevisibleiQ9Z1Z0. MM.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR006955. Uso1_p115_C.
IPR024095. Vesicle_P115-like.
IPR006953. Vesicle_Uso1_P115_head.
[Graphical view]
PANTHERiPTHR10013. PTHR10013. 1 hit.
PfamiPF04871. Uso1_p115_C. 1 hit.
PF04869. Uso1_p115_head. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4).
    Strain: C57BL/6J and NOD.
    Tissue: Liver, Lung and Spleen.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Eye and Mammary tumor.
  3. "Identification of mouse TAP (transcytosis associated protein/p115)."
    Han S.J.
    Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-959 (ISOFORM 1).
    Strain: 129.
    Tissue: Thymus.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-940, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-940, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiUSO1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1Z0
Secondary accession number(s): Q3T9L9
, Q3TH58, Q3U1C7, Q3UMW6, Q91WE7, Q99JZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: January 10, 2006
Last modified: June 8, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.