Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

General vesicular transport factor p115

Gene

Uso1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.By similarity

GO - Molecular functioni

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • Golgi organization Source: MGI
  • Golgi vesicle docking Source: GO_Central
  • intracellular protein transport Source: MGI
  • membrane fusion Source: GO_Central
  • transcytosis Source: GO_Central
  • vesicle fusion with Golgi apparatus Source: InterPro
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-6807878. COPI-mediated anterograde transport.

Names & Taxonomyi

Protein namesi
Recommended name:
General vesicular transport factor p115
Alternative name(s):
Protein USO1 homolog
Transcytosis-associated protein
Short name:
TAP
Vesicle-docking protein
Gene namesi
Name:Uso1
Synonyms:Vdp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1929095. Uso1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000657751 – 959General vesicular transport factor p115Add BLAST959

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50PhosphoserineBy similarity1
Modified residuei202N6-acetyllysineBy similarity1
Modified residuei940PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promostes dissociation (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Z1Z0.
PaxDbiQ9Z1Z0.
PeptideAtlasiQ9Z1Z0.
PRIDEiQ9Z1Z0.

PTM databases

iPTMnetiQ9Z1Z0.
PhosphoSitePlusiQ9Z1Z0.
SwissPalmiQ9Z1Z0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029407.
CleanExiMM_USO1.
ExpressionAtlasiQ9Z1Z0. baseline and differential.
GenevisibleiQ9Z1Z0. MM.

Interactioni

Subunit structurei

Homodimer. Dimerizes by parallel association of the tails, resulting in an elongated structure with two globular head domains side by side, and a long rod-like tail structure. Interacts with MIF (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207788. 108 interactors.
IntActiQ9Z1Z0. 112 interactors.
MINTiMINT-1864334.
STRINGi10090.ENSMUSP00000031355.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1Z0.
SMRiQ9Z1Z0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati20 – 60ARM 1Add BLAST41
Repeati61 – 121ARM 2Add BLAST61
Repeati123 – 163ARM 3Add BLAST41
Repeati166 – 207ARM 4Add BLAST42
Repeati208 – 253ARM 5Add BLAST46
Repeati255 – 310ARM 6Add BLAST56
Repeati311 – 354ARM 7Add BLAST44
Repeati363 – 408ARM 8Add BLAST46
Repeati420 – 459ARM 9Add BLAST40
Repeati473 – 513ARM 10Add BLAST41
Repeati518 – 571ARM 11Add BLAST54
Repeati573 – 630ARM 12Add BLAST58

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 637Globular headAdd BLAST637

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili638 – 930Sequence analysisAdd BLAST293

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi935 – 959Asp/Glu-rich (acidic)Add BLAST25

Domaini

Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.

Sequence similaritiesi

Belongs to the VDP/USO1/EDE1 family.Curated
Contains 12 ARM repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0946. Eukaryota.
ENOG410XRCG. LUCA.
GeneTreeiENSGT00390000017018.
HOVERGENiHBG018067.
InParanoidiQ9Z1Z0.
OMAiGYQVNQD.
OrthoDBiEOG091G046H.
PhylomeDBiQ9Z1Z0.
TreeFamiTF106157.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR006955. Uso1_p115_C.
IPR024095. Vesicle_P115-like.
IPR006953. Vesicle_Uso1_P115_head.
[Graphical view]
PANTHERiPTHR10013. PTHR10013. 1 hit.
PfamiPF04871. Uso1_p115_C. 1 hit.
PF04869. Uso1_p115_head. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z1Z0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNFLRGVMGG QSAGPQHTEA ETIQKLCDRV ASSTLLDDRR NAVRALKSLS
60 70 80 90 100
KKYRLEVGIQ AMEHLIHVLQ TDRSDSEIIA YALDTLYNII SNDEEEELEE
110 120 130 140 150
NSTRQSEDLG SQFTEIFIKQ PENVTLLLSL LEEFDFHVRW PGVRLLTSLL
160 170 180 190 200
KQLGPPVQQI ILVSPMGVSR LMDLLADSRE IIRNDGVLLL QALTRSNGAI
210 220 230 240 250
QKIVAFENAF ERLLDIITEE GNSDGGIVVE DCLILLQNLL KTNNSNQNFF
260 270 280 290 300
KEGSYIQRMK PWFEVGEENS GWSAQKVTNL HLMLQLVRVL VSPTNPPGAT
310 320 330 340 350
SSCQKAMFQC GLLQQLCTIL MATGIPADIL TETINTVSEV IRGCQVNQDY
360 370 380 390 400
FASVNAPSNP PRPAIVVLLM SMVNERQPFV LRCAVLYCFQ CFLYKNEKGQ
410 420 430 440 450
GEIVATLLPS TIDATGNSVS AGQLLCGGLF STDSLSNWCA AVALAHALQG
460 470 480 490 500
NATQKEQLLR VQLATSIGNP PVSLLQQCTN ILSQGSKIQT RVGLLMLLCT
510 520 530 540 550
WLSNCPIAVT HFLHNSANVP FLTGQIAENL GEEEQLVQGL CALLLGISIY
560 570 580 590 600
FNDNSLENYT KEKLKQLIEK RIGKENFIEK LGFISKHELY SRASQKPQPN
610 620 630 640 650
FPSPEYMIFD HEFTKLVKEL EGVITKAIYK SSEEDKKEEE VKKTLEQHDN
660 670 680 690 700
IVTHYKNMIR EQDLQLEELK QQVSTLKCQN EQLQTAVTQQ ASQIQQHKDQ
710 720 730 740 750
YNLLKVQLGK DNHHQGSHGD GAQVNGIQPE EISRLREEIE ELKSQQALLQ
760 770 780 790 800
GQLAEKDSLI ENLKSSQASG MSEQASATCP PRDPEQVAEL KQELTALKSQ
810 820 830 840 850
LCSQSLEITR LQTENCELLQ RAETLAKSVP VEGESEHVSA AKTTDVEGRL
860 870 880 890 900
SALLQETKEL KNEIKALSEE RTAIQKQLDS SNSTIAILQT EKDKLDLEVT
910 920 930 940 950
DSKKEQDDLL VLLADQDQKI LSLKSKLKDL GHPVEEEDES GDQEDDDDEI

DDGDKDQDI
Length:959
Mass (Da):106,983
Last modified:January 10, 2006 - v2
Checksum:i2C05DC35E899D81C
GO
Isoform 2 (identifier: Q9Z1Z0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     764-825: Missing.

Show »
Length:897
Mass (Da):100,195
Checksum:i676131AB4D52BF3B
GO
Isoform 3 (identifier: Q9Z1Z0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     485-496: GSKIQTRVGLLM → VRATGLLRGERQ
     497-959: Missing.

Note: No experimental confirmation available.
Show »
Length:496
Mass (Da):54,732
Checksum:i0987BCE5E38C6EEA
GO
Isoform 4 (identifier: Q9Z1Z0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-606: Missing.

Note: No experimental confirmation available.
Show »
Length:353
Mass (Da):39,816
Checksum:i3214EC66D1C50555
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti225G → R in AAC72967 (Ref. 3) Curated1
Sequence conflicti291V → G in AAC72967 (Ref. 3) Curated1
Sequence conflicti377 – 378QP → HA in AAC72967 (Ref. 3) Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0169781 – 606Missing in isoform 4. 1 PublicationAdd BLAST606
Alternative sequenceiVSP_016979485 – 496GSKIQ…VGLLM → VRATGLLRGERQ in isoform 3. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_016980497 – 959Missing in isoform 3. 1 PublicationAdd BLAST463
Alternative sequenceiVSP_016981764 – 825Missing in isoform 2. 1 PublicationAdd BLAST62

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144638 mRNA. Translation: BAE25982.1.
AK156070 mRNA. Translation: BAE33571.1.
AK168433 mRNA. Translation: BAE40340.1.
AK172425 mRNA. Translation: BAE43001.1.
BC005548 mRNA. Translation: AAH05548.1.
BC016069 mRNA. Translation: AAH16069.1.
AF096868 mRNA. Translation: AAC72967.1.
CCDSiCCDS39150.1. [Q9Z1Z0-1]
RefSeqiNP_062363.1. NM_019490.1. [Q9Z1Z0-1]
UniGeneiMm.15868.

Genome annotation databases

EnsembliENSMUST00000031355; ENSMUSP00000031355; ENSMUSG00000029407. [Q9Z1Z0-1]
ENSMUST00000202155; ENSMUSP00000144592; ENSMUSG00000029407. [Q9Z1Z0-2]
GeneIDi56041.
KEGGimmu:56041.
UCSCiuc008ycl.1. mouse. [Q9Z1Z0-3]
uc008ycm.1. mouse. [Q9Z1Z0-1]
uc008ycn.1. mouse. [Q9Z1Z0-4]
uc012dyl.1. mouse. [Q9Z1Z0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144638 mRNA. Translation: BAE25982.1.
AK156070 mRNA. Translation: BAE33571.1.
AK168433 mRNA. Translation: BAE40340.1.
AK172425 mRNA. Translation: BAE43001.1.
BC005548 mRNA. Translation: AAH05548.1.
BC016069 mRNA. Translation: AAH16069.1.
AF096868 mRNA. Translation: AAC72967.1.
CCDSiCCDS39150.1. [Q9Z1Z0-1]
RefSeqiNP_062363.1. NM_019490.1. [Q9Z1Z0-1]
UniGeneiMm.15868.

3D structure databases

ProteinModelPortaliQ9Z1Z0.
SMRiQ9Z1Z0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207788. 108 interactors.
IntActiQ9Z1Z0. 112 interactors.
MINTiMINT-1864334.
STRINGi10090.ENSMUSP00000031355.

PTM databases

iPTMnetiQ9Z1Z0.
PhosphoSitePlusiQ9Z1Z0.
SwissPalmiQ9Z1Z0.

Proteomic databases

EPDiQ9Z1Z0.
PaxDbiQ9Z1Z0.
PeptideAtlasiQ9Z1Z0.
PRIDEiQ9Z1Z0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031355; ENSMUSP00000031355; ENSMUSG00000029407. [Q9Z1Z0-1]
ENSMUST00000202155; ENSMUSP00000144592; ENSMUSG00000029407. [Q9Z1Z0-2]
GeneIDi56041.
KEGGimmu:56041.
UCSCiuc008ycl.1. mouse. [Q9Z1Z0-3]
uc008ycm.1. mouse. [Q9Z1Z0-1]
uc008ycn.1. mouse. [Q9Z1Z0-4]
uc012dyl.1. mouse. [Q9Z1Z0-2]

Organism-specific databases

CTDi8615.
MGIiMGI:1929095. Uso1.

Phylogenomic databases

eggNOGiKOG0946. Eukaryota.
ENOG410XRCG. LUCA.
GeneTreeiENSGT00390000017018.
HOVERGENiHBG018067.
InParanoidiQ9Z1Z0.
OMAiGYQVNQD.
OrthoDBiEOG091G046H.
PhylomeDBiQ9Z1Z0.
TreeFamiTF106157.

Enzyme and pathway databases

ReactomeiR-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-6807878. COPI-mediated anterograde transport.

Miscellaneous databases

ChiTaRSiUso1. mouse.
PROiQ9Z1Z0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029407.
CleanExiMM_USO1.
ExpressionAtlasiQ9Z1Z0. baseline and differential.
GenevisibleiQ9Z1Z0. MM.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR006955. Uso1_p115_C.
IPR024095. Vesicle_P115-like.
IPR006953. Vesicle_Uso1_P115_head.
[Graphical view]
PANTHERiPTHR10013. PTHR10013. 1 hit.
PfamiPF04871. Uso1_p115_C. 1 hit.
PF04869. Uso1_p115_head. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUSO1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1Z0
Secondary accession number(s): Q3T9L9
, Q3TH58, Q3U1C7, Q3UMW6, Q91WE7, Q99JZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: January 10, 2006
Last modified: November 2, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.