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Protein

Type II iodothyronine deiodinase

Gene

Dio2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.By similarity

Catalytic activityi

3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.PROSITE-ProRule annotation

Kineticsi

  1. KM=4.4 nM for T41 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei1301

    GO - Molecular functioni

    • thyroxine 5'-deiodinase activity Source: UniProtKB
    • ubiquitin protein ligase binding Source: MGI

    GO - Biological processi

    • hormone biosynthetic process Source: UniProtKB-KW
    • thyroid hormone catabolic process Source: MGI
    • thyroid hormone metabolic process Source: MGI
    • thyroid-stimulating hormone secretion Source: UniProtKB

    Keywordsi

    Molecular functionOxidoreductase
    Biological processThyroid hormones biosynthesis

    Enzyme and pathway databases

    BRENDAi1.97.1.10 3474
    ReactomeiR-MMU-350864 Regulation of thyroid hormone activity
    SABIO-RKiQ9Z1Y9

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type II iodothyronine deiodinase (EC:1.21.99.4)
    Alternative name(s):
    5DII
    DIOII
    Type 2 DI
    Type-II 5'-deiodinase
    Gene namesi
    Name:Dio2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 12

    Organism-specific databases

    MGIiMGI:1338833 Dio2

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei10 – 34HelicalSequence analysisAdd BLAST25

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001543181 – 266Type II iodothyronine deiodinaseAdd BLAST266

    Post-translational modificationi

    Ubiquitinated by MARCH6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33 (By similarity).By similarity

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PaxDbiQ9Z1Y9
    PRIDEiQ9Z1Y9

    Expressioni

    Tissue specificityi

    Expressed in mammary gland and in brain.1 Publication

    Gene expression databases

    BgeeiENSMUSG00000007682
    CleanExiMM_DIO2
    GenevisibleiQ9Z1Y9 MM

    Interactioni

    Subunit structurei

    Interacts with USP20 and USP33. Interacts with MARCH6 (By similarity).By similarity

    GO - Molecular functioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000081013

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z1Y9
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the iodothyronine deiodinase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IXX0 Eukaryota
    ENOG4111SVE LUCA
    GeneTreeiENSGT00820000127068
    HOGENOMiHOG000007088
    HOVERGENiHBG000099
    InParanoidiQ9Z1Y9
    KOiK17904
    OMAiAFERVCI
    OrthoDBiEOG091G0V5C
    PhylomeDBiQ9Z1Y9
    TreeFamiTF329721

    Family and domain databases

    InterProiView protein in InterPro
    IPR000643 Iodothyronine_deiodinase
    IPR008261 Iodothyronine_deiodinase_AS
    IPR036249 Thioredoxin-like_sf
    PANTHERiPTHR11781 PTHR11781, 1 hit
    PfamiView protein in Pfam
    PF00837 T4_deiodinase, 1 hit
    PIRSFiPIRSF001330 IOD, 1 hit
    SUPFAMiSSF52833 SSF52833, 1 hit
    PROSITEiView protein in PROSITE
    PS01205 T4_DEIODINASE, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9Z1Y9-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGLLSVDLLI TLQILPVFFS NCLFLALYDS VILLKHVALL LSRSKSTRGE
    60 70 80 90 100
    WRRMLTSEGL RCVWNSFLLD AYKQVKLGED APNSSVVHVS NPESGNNYAS
    110 120 130 140 150
    EKTADGAECH LLDFASAERP LVVNFGSATU PPFTRQLPAF RQLVEEFSSV
    160 170 180 190 200
    ADFLLVYIDE AHPSDGWAVP GDSSLSFEVK KHRNQEDRCA AAHQLLERFS
    210 220 230 240 250
    LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG KGPFSYNLQE
    260
    VRSWLEKNFS KRUILD
    Length:266
    Mass (Da):29,929
    Last modified:February 26, 2008 - v4
    Checksum:iB2FD28E171DC5486
    GO

    Non-standard residue

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Non-standard residuei130Selenocysteine1
    Non-standard residuei263Selenocysteine1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF096875 mRNA Translation: AAD11422.1
    AF177196 mRNA Translation: AAD53113.1
    AF093137 mRNA Translation: AAF00069.2
    BC125383 mRNA Translation: AAI25384.1
    BC125385 mRNA Translation: AAI25386.1
    CCDSiCCDS36514.1
    RefSeqiNP_034180.1, NM_010050.3
    UniGeneiMm.21389
    Mm.408815

    Genome annotation databases

    EnsembliENSMUST00000082432; ENSMUSP00000081013; ENSMUSG00000007682
    GeneIDi13371
    KEGGimmu:13371
    UCSCiuc007okf.1 mouse

    Keywords - Coding sequence diversityi

    Selenocysteine

    Similar proteinsi

    Entry informationi

    Entry nameiIOD2_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z1Y9
    Secondary accession number(s): Q05A70, Q9JHH1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: February 26, 2008
    Last modified: March 28, 2018
    This is version 136 of the entry and version 4 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

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