Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Type II iodothyronine deiodinase

Gene

Dio2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development (By similarity).By similarity

Catalytic activityi

3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.PROSITE-ProRule annotation

Kineticsi

  1. KM=1.1 nM for T41 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei130 – 1301

GO - Molecular functioni

  1. thyroxine 5'-deiodinase activity Source: UniProtKB
  2. ubiquitin protein ligase binding Source: MGI

GO - Biological processi

  1. hormone biosynthetic process Source: UniProtKB-KW
  2. thyroid hormone catabolic process Source: MGI
  3. thyroid hormone metabolic process Source: MGI
  4. thyroid-stimulating hormone secretion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Thyroid hormones biosynthesis

Enzyme and pathway databases

BRENDAi1.97.1.10. 3474.
ReactomeiREACT_210325. Regulation of thyroid hormone activity.

Names & Taxonomyi

Protein namesi
Recommended name:
Type II iodothyronine deiodinase (EC:1.97.1.10)
Alternative name(s):
5DII
DIOII
Type 2 DI
Type-II 5'-deiodinase
Gene namesi
Name:Dio2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:1338833. Dio2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 3425HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 266266Type II iodothyronine deiodinasePRO_0000154318Add
BLAST

Post-translational modificationi

Ubiquitinated by MARCH6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33 (By similarity).By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiQ9Z1Y9.

Expressioni

Tissue specificityi

Expressed in mammary gland and in brain.1 Publication

Gene expression databases

BgeeiQ9Z1Y9.
CleanExiMM_DIO2.
ExpressionAtlasiQ9Z1Y9. baseline and differential.
GenevestigatoriQ9Z1Y9.

Interactioni

Subunit structurei

Interacts with USP20 and USP33. Interacts with MARCH6 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9Z1Y9.
SMRiQ9Z1Y9. Positions 69-257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the iodothyronine deiodinase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG146259.
GeneTreeiENSGT00730000110840.
HOGENOMiHOG000007088.
HOVERGENiHBG000099.
InParanoidiQ9Z1Y9.
KOiK17904.
OMAiAFERVCI.
OrthoDBiEOG7XPZ7H.
PhylomeDBiQ9Z1Y9.
TreeFamiTF329721.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000643. Iodothyronine_deiodinase.
IPR008261. Iodothyronine_deiodinase_AS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11781. PTHR11781. 1 hit.
PfamiPF00837. T4_deiodinase. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS01205. T4_DEIODINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z1Y9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLSVDLLI TLQILPVFFS NCLFLALYDS VILLKHVALL LSRSKSTRGE
60 70 80 90 100
WRRMLTSEGL RCVWNSFLLD AYKQVKLGED APNSSVVHVS NPESGNNYAS
110 120 130 140 150
EKTADGAECH LLDFASAERP LVVNFGSATU PPFTRQLPAF RQLVEEFSSV
160 170 180 190 200
ADFLLVYIDE AHPSDGWAVP GDSSLSFEVK KHRNQEDRCA AAHQLLERFS
210 220 230 240 250
LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG KGPFSYNLQE
260
VRSWLEKNFS KRUILD
Length:266
Mass (Da):29,929
Last modified:February 26, 2008 - v4
Checksum:iB2FD28E171DC5486
GO

Non-standard residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-standard residuei130 – 1301Selenocysteine
Non-standard residuei263 – 2631Selenocysteine

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF096875 mRNA. Translation: AAD11422.1.
AF177196 mRNA. Translation: AAD53113.1.
AF093137 mRNA. Translation: AAF00069.2.
BC125383 mRNA. Translation: AAI25384.1.
BC125385 mRNA. Translation: AAI25386.1.
CCDSiCCDS36514.1.
RefSeqiNP_034180.1. NM_010050.2.
UniGeneiMm.21389.
Mm.408815.

Genome annotation databases

EnsembliENSMUST00000082432; ENSMUSP00000081013; ENSMUSG00000007682.
GeneIDi13371.
KEGGimmu:13371.
UCSCiuc007okf.1. mouse.

Keywords - Coding sequence diversityi

Selenocysteine

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF096875 mRNA. Translation: AAD11422.1.
AF177196 mRNA. Translation: AAD53113.1.
AF093137 mRNA. Translation: AAF00069.2.
BC125383 mRNA. Translation: AAI25384.1.
BC125385 mRNA. Translation: AAI25386.1.
CCDSiCCDS36514.1.
RefSeqiNP_034180.1. NM_010050.2.
UniGeneiMm.21389.
Mm.408815.

3D structure databases

ProteinModelPortaliQ9Z1Y9.
SMRiQ9Z1Y9. Positions 69-257.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9Z1Y9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000082432; ENSMUSP00000081013; ENSMUSG00000007682.
GeneIDi13371.
KEGGimmu:13371.
UCSCiuc007okf.1. mouse.

Organism-specific databases

CTDi1734.
MGIiMGI:1338833. Dio2.

Phylogenomic databases

eggNOGiNOG146259.
GeneTreeiENSGT00730000110840.
HOGENOMiHOG000007088.
HOVERGENiHBG000099.
InParanoidiQ9Z1Y9.
KOiK17904.
OMAiAFERVCI.
OrthoDBiEOG7XPZ7H.
PhylomeDBiQ9Z1Y9.
TreeFamiTF329721.

Enzyme and pathway databases

BRENDAi1.97.1.10. 3474.
ReactomeiREACT_210325. Regulation of thyroid hormone activity.

Miscellaneous databases

ChiTaRSiDio2. mouse.
NextBioi283720.
PROiQ9Z1Y9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z1Y9.
CleanExiMM_DIO2.
ExpressionAtlasiQ9Z1Y9. baseline and differential.
GenevestigatoriQ9Z1Y9.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000643. Iodothyronine_deiodinase.
IPR008261. Iodothyronine_deiodinase_AS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11781. PTHR11781. 1 hit.
PfamiPF00837. T4_deiodinase. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS01205. T4_DEIODINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a 5.8 kb cDNA for a mouse type 2 deiodinase."
    Davey J.C., Schneider M.J., Becker K.B., Galton V.A.
    Endocrinology 140:1022-1025(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Biochemical and molecular biological evidence for the presence of type II iodothyronine deiodinase in mouse mammary gland."
    Song S., Sorimachi K., Adachi K., Oka T.
    Mol. Cell. Endocrinol. 160:173-181(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    Strain: C3H/HeN.
    Tissue: Brain.
  3. "Type 2 iodothyronine deiodinase expression in the cochlea before the onset of hearing."
    Campos-Barros A., Amma L.L., Faris J.S., Shailam R., Kelley M.W., Forrest D.
    Proc. Natl. Acad. Sci. U.S.A. 97:1287-1292(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Cochlea.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiIOD2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1Y9
Secondary accession number(s): Q05A70, Q9JHH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 26, 2008
Last modified: February 4, 2015
This is version 118 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.