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Protein

Type II iodothyronine deiodinase

Gene

Dio2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development (By similarity).By similarity

Catalytic activityi

3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.PROSITE-ProRule annotation

Kineticsi

  1. KM=1.1 nM for T41 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei130 – 1301

    GO - Molecular functioni

    • thyroxine 5'-deiodinase activity Source: UniProtKB
    • ubiquitin protein ligase binding Source: MGI

    GO - Biological processi

    • hormone biosynthetic process Source: UniProtKB-KW
    • thyroid hormone catabolic process Source: MGI
    • thyroid hormone metabolic process Source: MGI
    • thyroid-stimulating hormone secretion Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Thyroid hormones biosynthesis

    Enzyme and pathway databases

    BRENDAi1.97.1.10. 3474.
    ReactomeiREACT_294831. Regulation of thyroid hormone activity.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type II iodothyronine deiodinase (EC:1.97.1.10)
    Alternative name(s):
    5DII
    DIOII
    Type 2 DI
    Type-II 5'-deiodinase
    Gene namesi
    Name:Dio2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589 Componenti: Chromosome 12

    Organism-specific databases

    MGIiMGI:1338833. Dio2.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei10 – 3425HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    • cell Source: GOC
    • integral component of membrane Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 266266Type II iodothyronine deiodinasePRO_0000154318Add
    BLAST

    Post-translational modificationi

    Ubiquitinated by MARCH6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33 (By similarity).By similarity

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PRIDEiQ9Z1Y9.

    Expressioni

    Tissue specificityi

    Expressed in mammary gland and in brain.1 Publication

    Gene expression databases

    BgeeiQ9Z1Y9.
    CleanExiMM_DIO2.
    ExpressionAtlasiQ9Z1Y9. baseline and differential.
    GenevisibleiQ9Z1Y9. MM.

    Interactioni

    Subunit structurei

    Interacts with USP20 and USP33. Interacts with MARCH6 (By similarity).By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000081013.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z1Y9.
    SMRiQ9Z1Y9. Positions 69-257.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the iodothyronine deiodinase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG146259.
    GeneTreeiENSGT00730000110840.
    HOGENOMiHOG000007088.
    HOVERGENiHBG000099.
    InParanoidiQ9Z1Y9.
    KOiK17904.
    OMAiAFERVCI.
    OrthoDBiEOG7XPZ7H.
    PhylomeDBiQ9Z1Y9.
    TreeFamiTF329721.

    Family and domain databases

    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR000643. Iodothyronine_deiodinase.
    IPR008261. Iodothyronine_deiodinase_AS.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PANTHERiPTHR11781. PTHR11781. 1 hit.
    PfamiPF00837. T4_deiodinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001330. IOD. 1 hit.
    SUPFAMiSSF52833. SSF52833. 1 hit.
    PROSITEiPS01205. T4_DEIODINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z1Y9-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGLLSVDLLI TLQILPVFFS NCLFLALYDS VILLKHVALL LSRSKSTRGE
    60 70 80 90 100
    WRRMLTSEGL RCVWNSFLLD AYKQVKLGED APNSSVVHVS NPESGNNYAS
    110 120 130 140 150
    EKTADGAECH LLDFASAERP LVVNFGSATU PPFTRQLPAF RQLVEEFSSV
    160 170 180 190 200
    ADFLLVYIDE AHPSDGWAVP GDSSLSFEVK KHRNQEDRCA AAHQLLERFS
    210 220 230 240 250
    LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG KGPFSYNLQE
    260
    VRSWLEKNFS KRUILD
    Length:266
    Mass (Da):29,929
    Last modified:February 26, 2008 - v4
    Checksum:iB2FD28E171DC5486
    GO

    Non-standard residue

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-standard residuei130 – 1301Selenocysteine
    Non-standard residuei263 – 2631Selenocysteine

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF096875 mRNA. Translation: AAD11422.1.
    AF177196 mRNA. Translation: AAD53113.1.
    AF093137 mRNA. Translation: AAF00069.2.
    BC125383 mRNA. Translation: AAI25384.1.
    BC125385 mRNA. Translation: AAI25386.1.
    CCDSiCCDS36514.1.
    RefSeqiNP_034180.1. NM_010050.2.
    UniGeneiMm.21389.
    Mm.408815.

    Genome annotation databases

    EnsembliENSMUST00000082432; ENSMUSP00000081013; ENSMUSG00000007682.
    GeneIDi13371.
    KEGGimmu:13371.
    UCSCiuc007okf.1. mouse.

    Keywords - Coding sequence diversityi

    Selenocysteine

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF096875 mRNA. Translation: AAD11422.1.
    AF177196 mRNA. Translation: AAD53113.1.
    AF093137 mRNA. Translation: AAF00069.2.
    BC125383 mRNA. Translation: AAI25384.1.
    BC125385 mRNA. Translation: AAI25386.1.
    CCDSiCCDS36514.1.
    RefSeqiNP_034180.1. NM_010050.2.
    UniGeneiMm.21389.
    Mm.408815.

    3D structure databases

    ProteinModelPortaliQ9Z1Y9.
    SMRiQ9Z1Y9. Positions 69-257.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000081013.

    Proteomic databases

    PRIDEiQ9Z1Y9.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000082432; ENSMUSP00000081013; ENSMUSG00000007682.
    GeneIDi13371.
    KEGGimmu:13371.
    UCSCiuc007okf.1. mouse.

    Organism-specific databases

    CTDi1734.
    MGIiMGI:1338833. Dio2.

    Phylogenomic databases

    eggNOGiNOG146259.
    GeneTreeiENSGT00730000110840.
    HOGENOMiHOG000007088.
    HOVERGENiHBG000099.
    InParanoidiQ9Z1Y9.
    KOiK17904.
    OMAiAFERVCI.
    OrthoDBiEOG7XPZ7H.
    PhylomeDBiQ9Z1Y9.
    TreeFamiTF329721.

    Enzyme and pathway databases

    BRENDAi1.97.1.10. 3474.
    ReactomeiREACT_294831. Regulation of thyroid hormone activity.

    Miscellaneous databases

    ChiTaRSiDio2. mouse.
    NextBioi283720.
    PROiQ9Z1Y9.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ9Z1Y9.
    CleanExiMM_DIO2.
    ExpressionAtlasiQ9Z1Y9. baseline and differential.
    GenevisibleiQ9Z1Y9. MM.

    Family and domain databases

    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR000643. Iodothyronine_deiodinase.
    IPR008261. Iodothyronine_deiodinase_AS.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PANTHERiPTHR11781. PTHR11781. 1 hit.
    PfamiPF00837. T4_deiodinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001330. IOD. 1 hit.
    SUPFAMiSSF52833. SSF52833. 1 hit.
    PROSITEiPS01205. T4_DEIODINASE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Cloning of a 5.8 kb cDNA for a mouse type 2 deiodinase."
      Davey J.C., Schneider M.J., Becker K.B., Galton V.A.
      Endocrinology 140:1022-1025(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Biochemical and molecular biological evidence for the presence of type II iodothyronine deiodinase in mouse mammary gland."
      Song S., Sorimachi K., Adachi K., Oka T.
      Mol. Cell. Endocrinol. 160:173-181(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
      Strain: C3H/HeN.
      Tissue: Brain.
    3. "Type 2 iodothyronine deiodinase expression in the cochlea before the onset of hearing."
      Campos-Barros A., Amma L.L., Faris J.S., Shailam R., Kelley M.W., Forrest D.
      Proc. Natl. Acad. Sci. U.S.A. 97:1287-1292(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Cochlea.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.

    Entry informationi

    Entry nameiIOD2_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z1Y9
    Secondary accession number(s): Q05A70, Q9JHH1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: February 26, 2008
    Last modified: June 24, 2015
    This is version 120 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.