Q9Z1Y9 (IOD2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type II iodothyronine deiodinase
EC=1.97.1.10

Alternative name(s):
5DII
DIOII
Type 2 DI
Type-II 5'-deiodinase

Gene names

Name:

Dio2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development By similarity.
Catalytic activity	3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.
Subunit structure	Interacts with USP20 and USP33. Interacts with MARCH6 By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Tissue specificity	Expressed in mammary gland and in brain. Ref.2
Post-translational modification	Ubiquitinated by MARCH6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33 By similarity.
Sequence similarities	Belongs to the iodothyronine deiodinase family.
Biophysicochemical properties	Kinetic parameters: KM=1.1 nM for T4 Ref.2

Ontologies

Keywords
Biological process	Thyroid hormones biosynthesis
Cellular component	Membrane
Coding sequence diversity	Selenocysteine
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW thyroid hormone catabolic process Inferred from mutant phenotype PubMed 11731615. Source: MGI thyroid-stimulating hormone secretion Traceable author statement PubMed 12586753. Source: UniProtKB
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	thyroxine 5'-deiodinase activity Inferred from direct assay PubMed 12586753. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Type II iodothyronine deiodinase		PRO_0000154318
Regions
	Transmembrane	10 – 34	25	Helical; Potential
Sites
	Active site	130	1
Amino acid modifications
	Non-standard residue	130	1	Selenocysteine
	Non-standard residue	263	1	Selenocysteine

Sequences

	Sequence		Length	Mass (Da)
	Q9Z1Y9 [UniParc]. Last modified February 26, 2008. Version 4. Checksum: B2FD28E171DC5486	FASTA	266	29,929
	10 20 30 40 50 60 MGLLSVDLLI TLQILPVFFS NCLFLALYDS VILLKHVALL LSRSKSTRGE WRRMLTSEGL 70 80 90 100 110 120 RCVWNSFLLD AYKQVKLGED APNSSVVHVS NPESGNNYAS EKTADGAECH LLDFASAERP 130 140 150 160 170 180 LVVNFGSATU PPFTRQLPAF RQLVEEFSSV ADFLLVYIDE AHPSDGWAVP GDSSLSFEVK 190 200 210 220 230 240 KHRNQEDRCA AAHQLLERFS LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG 250 260 KGPFSYNLQE VRSWLEKNFS KRUILD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a 5.8 kb cDNA for a mouse type 2 deiodinase." Davey J.C., Schneider M.J., Becker K.B., Galton V.A. Endocrinology 140:1022-1025(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Biochemical and molecular biological evidence for the presence of type II iodothyronine deiodinase in mouse mammary gland." Song S., Sorimachi K., Adachi K., Oka T. Mol. Cell. Endocrinol. 160:173-181(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY. Strain: C3H/HeN. Tissue: Brain.
[3]	"Type 2 iodothyronine deiodinase expression in the cochlea before the onset of hearing." Campos-Barros A., Amma L.L., Faris J.S., Shailam R., Kelley M.W., Forrest D. Proc. Natl. Acad. Sci. U.S.A. 97:1287-1292(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cochlea.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF096875 mRNA. Translation: AAD11422.1. AF177196 mRNA. Translation: AAD53113.1. AF093137 mRNA. Translation: AAF00069.2. BC125383 mRNA. Translation: AAI25384.1. BC125385 mRNA. Translation: AAI25386.1.
IPI	IPI00466494.
RefSeq	NP_034180.1. NM_010050.2.
UniGene	Mm.21389. Mm.408815.
3D structure databases
ProteinModelPortal	Q9Z1Y9.
ModBase	Search...
Proteomic databases
PRIDE	Q9Z1Y9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000082432; ENSMUSP00000081013; ENSMUSG00000007682.
GeneID	13371.
KEGG	mmu:13371.
UCSC	uc007okf.1. mouse.
Organism-specific databases
CTD	1734.
MGI	MGI:1338833. Dio2.
Phylogenomic databases
eggNOG	NOG146259.
GeneTree	ENSGT00700000104606.
HOGENOM	HOG000007088.
HOVERGEN	HBG000099.
InParanoid	Q9Z1Y9.
KO	K01562.
OMA	AFERVCI.
OrthoDB	EOG4X97HS.
Enzyme and pathway databases
BRENDA	1.97.1.10. 3474.
Gene expression databases
ArrayExpress	Q9Z1Y9.
Bgee	Q9Z1Y9.
CleanEx	MM_DIO2.
Genevestigator	Q9Z1Y9.
GermOnline	ENSMUSG00000007682. Mus musculus.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR000643. Iodothyronine_deiodinase. IPR008261. Iodothyronine_deiodinase_AS. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR11781. PTHR11781. 1 hit.
Pfam	PF00837. T4_deiodinase. 1 hit. [Graphical view]
PROSITE	PS01205. T4_DEIODINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	DIO2. mouse.
NextBio	283720.
SOURCE	Search...

Entry information

Entry name

IOD2_MOUSE

Accession

Primary (citable) accession number: Q9Z1Y9
Secondary accession number(s): Q05A70, Q9JHH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 26, 2008
Last modified:	April 3, 2013
This is version 104 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program