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Protein

Cadherin-2

Gene

Cdh2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi262 – 2621Calcium 1By similarity
Metal bindingi262 – 2621Calcium 2By similarity
Metal bindingi293 – 2931Calcium 3By similarity

GO - Molecular functioni

  • beta-catenin binding Source: RGD
  • calcium ion binding Source: InterPro
  • nitric-oxide synthase binding Source: RGD
  • protein complex binding Source: RGD
  • protein kinase binding Source: RGD

GO - Biological processi

  • calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules Source: RGD
  • cell-cell adhesion mediated by cadherin Source: UniProtKB
  • glial cell differentiation Source: UniProtKB
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: InterPro
  • neuronal stem cell population maintenance Source: UniProtKB
  • protein heterooligomerization Source: RGD
  • regulation of axonogenesis Source: RGD
  • regulation of myelination Source: RGD
  • regulation of protein localization Source: RGD
  • regulation of Rho protein signal transduction Source: RGD
  • single organismal cell-cell adhesion Source: RGD
  • synapse assembly Source: RGD
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cadherin-2
Alternative name(s):
Neural cadherin
Short name:
N-cadherin
CD_antigen: CD325
Gene namesi
Name:Cdh2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69280. Cdh2.

Subcellular locationi

  • Cell membrane By similarity; Single-pass type I membrane protein Sequence analysis

  • Note: Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini160 – 724565ExtracellularSequence analysisAdd
BLAST
Transmembranei725 – 74521HelicalSequence analysisAdd
BLAST
Topological domaini746 – 906161CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell-cell adherens junction Source: RGD
  • fascia adherens Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • intercalated disc Source: BHF-UCL
  • membrane Source: MGI
  • plasma membrane Source: BHF-UCL
  • protein complex Source: RGD
  • synapse Source: BHF-UCL
  • T-tubule Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi740 – 7401L → P: Decrease in PCDH8-binding; alone or when associated with G-741. 1 Publication
Mutagenesisi741 – 7411M → G: Decrease in PCDH8-binding; when associated with P-740. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Propeptidei26 – 159134Sequence analysisPRO_0000003735Add
BLAST
Chaini160 – 906747Cadherin-2PRO_0000003736Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei96 – 961PhosphoserineBy similarity
Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence analysis
Glycosylationi273 – 2731N-linked (GlcNAc...)Sequence analysis
Glycosylationi325 – 3251N-linked (GlcNAc...)Sequence analysis
Glycosylationi402 – 4021N-linked (GlcNAc...)Sequence analysis
Glycosylationi572 – 5721N-linked (GlcNAc...)Sequence analysis
Glycosylationi622 – 6221N-linked (GlcNAc...)Sequence analysis
Glycosylationi651 – 6511N-linked (GlcNAc...)Sequence analysis
Glycosylationi692 – 6921N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9Z1Y3.
PRIDEiQ9Z1Y3.

PTM databases

iPTMnetiQ9Z1Y3.
PhosphoSiteiQ9Z1Y3.
SwissPalmiQ9Z1Y3.
UniCarbKBiQ9Z1Y3.

Expressioni

Tissue specificityi

In testis, expressed in Sertoli and germ cells.1 Publication

Interactioni

Subunit structurei

Interacts with CDCP1. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN (By similarity). Interacts with PCDH8; this complex may also include TAOK2. The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway.By similarity1 Publication

GO - Molecular functioni

  • beta-catenin binding Source: RGD
  • nitric-oxide synthase binding Source: RGD
  • protein complex binding Source: RGD
  • protein kinase binding Source: RGD

Protein-protein interaction databases

BioGridi249720. 1 interaction.
DIPiDIP-48902N.
STRINGi10116.ENSRNOP00000021170.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1Y3.
SMRiQ9Z1Y3. Positions 24-374, 836-901.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini160 – 267108Cadherin 1PROSITE-ProRule annotationAdd
BLAST
Domaini268 – 382115Cadherin 2PROSITE-ProRule annotationAdd
BLAST
Domaini383 – 497115Cadherin 3PROSITE-ProRule annotationAdd
BLAST
Domaini498 – 603106Cadherin 4PROSITE-ProRule annotationAdd
BLAST
Domaini604 – 717114Cadherin 5PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi863 – 87816Ser-richAdd
BLAST

Domaini

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. Calcium-binding sites are occupied sequentially in the order of site 3, then site 2 and site 1 (By similarity).By similarity

Sequence similaritiesi

Contains 5 cadherin domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3594. Eukaryota.
ENOG410XQHI. LUCA.
HOGENOMiHOG000231254.
HOVERGENiHBG106438.
InParanoidiQ9Z1Y3.
KOiK06736.
PhylomeDBiQ9Z1Y3.

Family and domain databases

Gene3Di2.60.40.60. 6 hits.
4.10.900.10. 1 hit.
InterProiIPR002126. Cadherin.
IPR015919. Cadherin-like.
IPR020894. Cadherin_CS.
IPR000233. Cadherin_cytoplasmic-dom.
IPR014868. Cadherin_pro_dom.
IPR027397. Catenin_binding_dom.
IPR030051. CDH2.
[Graphical view]
PANTHERiPTHR24027:SF79. PTHR24027:SF79. 1 hit.
PfamiPF00028. Cadherin. 5 hits.
PF01049. Cadherin_C. 1 hit.
PF08758. Cadherin_pro. 1 hit.
[Graphical view]
PRINTSiPR00205. CADHERIN.
SMARTiSM00112. CA. 5 hits.
SM01055. Cadherin_pro. 1 hit.
[Graphical view]
SUPFAMiSSF49313. SSF49313. 6 hits.
PROSITEiPS00232. CADHERIN_1. 3 hits.
PS50268. CADHERIN_2. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z1Y3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCRIAGGPRT LLPLLAALLQ ASLEASGELA LCKTGFPEDV YSAVLPKTVH
60 70 80 90 100
EGQPLLNVKF SNCNRKRKVQ YESSEPADFK VDEDGTVYAV RSFPLSAEQA
110 120 130 140 150
KFLIYAQDKE TQEKWQVAVN LSLEPSLTEE PMKEPHEIEE IVFPRQLAKH
160 170 180 190 200
SGALQRQKRD WVIPPINLPE NSRGPFPQEL VRIRSDRDKN LSLRYSVTGP
210 220 230 240 250
GADQPPTGIF IINPISGQLS VTKPLDRELI ARFHLRAHAV DINGNQVENP
260 270 280 290 300
IDIVINVIDM NDNRPEFLHQ VWNGSVPEGS KPGTYVMTVT AIDADDPNAL
310 320 330 340 350
NGMLRYRILS QAPSTPSPNM FTINNETGDI ITVAAGLDRE KVQQYTLIIQ
360 370 380 390 400
ATDMEGNPTY GLSNTATAVI TVTDVNDNPP EFTAMTFYGE VPENRVDVIV
410 420 430 440 450
ANLTVTDKDQ PHTPAWNAAY RISGGDPTGR FAILTDPNSN DGLVTVVKPI
460 470 480 490 500
DFETNRMFVL TVAAENQVPL AKGIQHPPQS TATVSVTVID VNENPYFAPN
510 520 530 540 550
PKIIRQEEGL HAGTMLTTLT AQDPDRYMQQ NIRYTKLSDP ANWLKIDPVN
560 570 580 590 600
GQITTIAVLD RESPNVKNNI YNATFLASDN GIPPMSGTGT LQIYLLDIND
610 620 630 640 650
NAPQVLPQEA ETCETPEPNS INITALDYDI DPNAGPFAFD LPLSPATIKR
660 670 680 690 700
NWTITRLNGD FAQLNLKIKF LEAGIYEVPI VITDSGNPPK SNISILRVKV
710 720 730 740 750
CQCDSNGDCT DVDRIVGAGL GTGTIIAILL CIIILLILVL MFVVWMKRRD
760 770 780 790 800
KERQAKQLLI DPEDDVRDNI LKYDEEGGGE EDQDYDLSQL QQPDTVEPDA
810 820 830 840 850
IKPVGIRRLD ERPIHAEPQY PVRSAAPHPG DIGDFINEGL KAADNDPTAP
860 870 880 890 900
PYDSLLVFDY EGSGSTAGSL SSLNSSSSGG DQDYDYLNDW GPRFKKLADM

YGGGDD
Length:906
Mass (Da):99,686
Last modified:May 1, 1999 - v1
Checksum:i97D9937FD8D1F8B5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71G → A in BAA84919 (Ref. 2) Curated
Sequence conflicti48 – 481T → D in BAA84919 (Ref. 2) Curated
Sequence conflicti153 – 1531A → R in BAA84919 (Ref. 2) Curated
Sequence conflicti646 – 6461A → V in BAA84919 (Ref. 2) Curated
Sequence conflicti658 – 6581N → K in BAA84919 (Ref. 2) Curated
Sequence conflicti724 – 7241T → A in BAA84919 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF097593 mRNA. Translation: AAC83818.1.
AB017695 mRNA. Translation: BAA84919.1.
RefSeqiNP_112623.1. NM_031333.1.
UniGeneiRn.23200.

Genome annotation databases

GeneIDi83501.
KEGGirno:83501.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF097593 mRNA. Translation: AAC83818.1.
AB017695 mRNA. Translation: BAA84919.1.
RefSeqiNP_112623.1. NM_031333.1.
UniGeneiRn.23200.

3D structure databases

ProteinModelPortaliQ9Z1Y3.
SMRiQ9Z1Y3. Positions 24-374, 836-901.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249720. 1 interaction.
DIPiDIP-48902N.
STRINGi10116.ENSRNOP00000021170.

PTM databases

iPTMnetiQ9Z1Y3.
PhosphoSiteiQ9Z1Y3.
SwissPalmiQ9Z1Y3.
UniCarbKBiQ9Z1Y3.

Proteomic databases

PaxDbiQ9Z1Y3.
PRIDEiQ9Z1Y3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83501.
KEGGirno:83501.

Organism-specific databases

CTDi1000.
RGDi69280. Cdh2.

Phylogenomic databases

eggNOGiKOG3594. Eukaryota.
ENOG410XQHI. LUCA.
HOGENOMiHOG000231254.
HOVERGENiHBG106438.
InParanoidiQ9Z1Y3.
KOiK06736.
PhylomeDBiQ9Z1Y3.

Miscellaneous databases

PROiQ9Z1Y3.

Family and domain databases

Gene3Di2.60.40.60. 6 hits.
4.10.900.10. 1 hit.
InterProiIPR002126. Cadherin.
IPR015919. Cadherin-like.
IPR020894. Cadherin_CS.
IPR000233. Cadherin_cytoplasmic-dom.
IPR014868. Cadherin_pro_dom.
IPR027397. Catenin_binding_dom.
IPR030051. CDH2.
[Graphical view]
PANTHERiPTHR24027:SF79. PTHR24027:SF79. 1 hit.
PfamiPF00028. Cadherin. 5 hits.
PF01049. Cadherin_C. 1 hit.
PF08758. Cadherin_pro. 1 hit.
[Graphical view]
PRINTSiPR00205. CADHERIN.
SMARTiSM00112. CA. 5 hits.
SM01055. Cadherin_pro. 1 hit.
[Graphical view]
SUPFAMiSSF49313. SSF49313. 6 hits.
PROSITEiPS00232. CADHERIN_1. 3 hits.
PS50268. CADHERIN_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Rat testicular N-cadherin: its complementary deoxyribonucleic acid cloning and regulation."
    Chung S.S., Mo M.Y., Silvestrini B., Lee W.M., Cheng C.Y.
    Endocrinology 139:1853-1862(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. "Rat N-cadherin cDNA."
    Asai K., Tada T., Yamamoto M., Tada A., Mizuno M., Eimoto T., Kato T.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. "Activity-induced protocadherin arcadlin regulates dendritic spine number by triggering N-cadherin endocytosis via TAO2beta and p38 MAP kinases."
    Yasuda S., Tanaka H., Sugiura H., Okamura K., Sakaguchi T., Tran U., Takemiya T., Mizoguchi A., Yagita Y., Sakurai T., De Robertis E.M., Yamagata K.
    Neuron 56:456-471(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PCDH8, MUTAGENESIS OF LEU-740 AND MET-741.

Entry informationi

Entry nameiCADH2_RAT
AccessioniPrimary (citable) accession number: Q9Z1Y3
Secondary accession number(s): Q9R0T5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 1999
Last modified: June 8, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.