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Protein

Cadherin-2

Gene

Cdh2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi262Calcium 1By similarity1
Metal bindingi262Calcium 2By similarity1
Metal bindingi293Calcium 3By similarity1

GO - Molecular functioni

  • beta-catenin binding Source: RGD
  • calcium ion binding Source: InterPro
  • nitric-oxide synthase binding Source: RGD
  • protein complex binding Source: RGD
  • protein kinase binding Source: RGD

GO - Biological processi

  • calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules Source: RGD
  • cell-cell adhesion mediated by cadherin Source: UniProtKB
  • glial cell differentiation Source: UniProtKB
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: InterPro
  • neuronal stem cell population maintenance Source: UniProtKB
  • protein heterooligomerization Source: RGD
  • regulation of axonogenesis Source: RGD
  • regulation of myelination Source: RGD
  • regulation of protein localization Source: RGD
  • regulation of Rho protein signal transduction Source: RGD
  • single organismal cell-cell adhesion Source: RGD
  • synapse assembly Source: RGD
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cadherin-2
Alternative name(s):
Neural cadherin
Short name:
N-cadherin
CD_antigen: CD325
Gene namesi
Name:Cdh2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69280. Cdh2.

Subcellular locationi

  • Cell membrane By similarity; Single-pass type I membrane protein Sequence analysis

  • Note: Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini160 – 724ExtracellularSequence analysisAdd BLAST565
Transmembranei725 – 745HelicalSequence analysisAdd BLAST21
Topological domaini746 – 906CytoplasmicSequence analysisAdd BLAST161

GO - Cellular componenti

  • cell-cell adherens junction Source: RGD
  • fascia adherens Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • intercalated disc Source: BHF-UCL
  • membrane Source: MGI
  • plasma membrane Source: BHF-UCL
  • protein complex Source: RGD
  • synapse Source: BHF-UCL
  • T-tubule Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi740L → P: Decrease in PCDH8-binding; alone or when associated with G-741. 1 Publication1
Mutagenesisi741M → G: Decrease in PCDH8-binding; when associated with P-740. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
PropeptideiPRO_000000373526 – 159Sequence analysisAdd BLAST134
ChainiPRO_0000003736160 – 906Cadherin-2Add BLAST747

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei96PhosphoserineBy similarity1
Glycosylationi190N-linked (GlcNAc...)Sequence analysis1
Glycosylationi273N-linked (GlcNAc...)Sequence analysis1
Glycosylationi325N-linked (GlcNAc...)Sequence analysis1
Glycosylationi402N-linked (GlcNAc...)Sequence analysis1
Glycosylationi572N-linked (GlcNAc...)Sequence analysis1
Glycosylationi622N-linked (GlcNAc...)Sequence analysis1
Glycosylationi651N-linked (GlcNAc...)Sequence analysis1
Glycosylationi692N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9Z1Y3.
PRIDEiQ9Z1Y3.

PTM databases

iPTMnetiQ9Z1Y3.
PhosphoSitePlusiQ9Z1Y3.
SwissPalmiQ9Z1Y3.
UniCarbKBiQ9Z1Y3.

Expressioni

Tissue specificityi

In testis, expressed in Sertoli and germ cells.1 Publication

Interactioni

Subunit structurei

Interacts with CDCP1. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN (By similarity). Interacts with PCDH8; this complex may also include TAOK2. The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway.By similarity1 Publication

GO - Molecular functioni

  • beta-catenin binding Source: RGD
  • nitric-oxide synthase binding Source: RGD
  • protein complex binding Source: RGD
  • protein kinase binding Source: RGD

Protein-protein interaction databases

BioGridi249720. 1 interactor.
DIPiDIP-48902N.
STRINGi10116.ENSRNOP00000021170.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1Y3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini160 – 267Cadherin 1PROSITE-ProRule annotationAdd BLAST108
Domaini268 – 382Cadherin 2PROSITE-ProRule annotationAdd BLAST115
Domaini383 – 497Cadherin 3PROSITE-ProRule annotationAdd BLAST115
Domaini498 – 603Cadherin 4PROSITE-ProRule annotationAdd BLAST106
Domaini604 – 717Cadherin 5PROSITE-ProRule annotationAdd BLAST114

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi863 – 878Ser-richAdd BLAST16

Domaini

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. Calcium-binding sites are occupied sequentially in the order of site 3, then site 2 and site 1 (By similarity).By similarity

Sequence similaritiesi

Contains 5 cadherin domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3594. Eukaryota.
ENOG410XQHI. LUCA.
HOGENOMiHOG000231254.
HOVERGENiHBG106438.
InParanoidiQ9Z1Y3.
KOiK06736.
PhylomeDBiQ9Z1Y3.

Family and domain databases

Gene3Di2.60.40.60. 6 hits.
4.10.900.10. 1 hit.
InterProiIPR002126. Cadherin.
IPR015919. Cadherin-like.
IPR020894. Cadherin_CS.
IPR000233. Cadherin_cytoplasmic-dom.
IPR014868. Cadherin_pro_dom.
IPR027397. Catenin_binding_dom.
IPR030051. CDH2.
[Graphical view]
PANTHERiPTHR24027:SF79. PTHR24027:SF79. 1 hit.
PfamiPF00028. Cadherin. 5 hits.
PF01049. Cadherin_C. 1 hit.
PF08758. Cadherin_pro. 1 hit.
[Graphical view]
PRINTSiPR00205. CADHERIN.
SMARTiSM00112. CA. 5 hits.
SM01055. Cadherin_pro. 1 hit.
[Graphical view]
SUPFAMiSSF49313. SSF49313. 6 hits.
PROSITEiPS00232. CADHERIN_1. 3 hits.
PS50268. CADHERIN_2. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z1Y3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCRIAGGPRT LLPLLAALLQ ASLEASGELA LCKTGFPEDV YSAVLPKTVH
60 70 80 90 100
EGQPLLNVKF SNCNRKRKVQ YESSEPADFK VDEDGTVYAV RSFPLSAEQA
110 120 130 140 150
KFLIYAQDKE TQEKWQVAVN LSLEPSLTEE PMKEPHEIEE IVFPRQLAKH
160 170 180 190 200
SGALQRQKRD WVIPPINLPE NSRGPFPQEL VRIRSDRDKN LSLRYSVTGP
210 220 230 240 250
GADQPPTGIF IINPISGQLS VTKPLDRELI ARFHLRAHAV DINGNQVENP
260 270 280 290 300
IDIVINVIDM NDNRPEFLHQ VWNGSVPEGS KPGTYVMTVT AIDADDPNAL
310 320 330 340 350
NGMLRYRILS QAPSTPSPNM FTINNETGDI ITVAAGLDRE KVQQYTLIIQ
360 370 380 390 400
ATDMEGNPTY GLSNTATAVI TVTDVNDNPP EFTAMTFYGE VPENRVDVIV
410 420 430 440 450
ANLTVTDKDQ PHTPAWNAAY RISGGDPTGR FAILTDPNSN DGLVTVVKPI
460 470 480 490 500
DFETNRMFVL TVAAENQVPL AKGIQHPPQS TATVSVTVID VNENPYFAPN
510 520 530 540 550
PKIIRQEEGL HAGTMLTTLT AQDPDRYMQQ NIRYTKLSDP ANWLKIDPVN
560 570 580 590 600
GQITTIAVLD RESPNVKNNI YNATFLASDN GIPPMSGTGT LQIYLLDIND
610 620 630 640 650
NAPQVLPQEA ETCETPEPNS INITALDYDI DPNAGPFAFD LPLSPATIKR
660 670 680 690 700
NWTITRLNGD FAQLNLKIKF LEAGIYEVPI VITDSGNPPK SNISILRVKV
710 720 730 740 750
CQCDSNGDCT DVDRIVGAGL GTGTIIAILL CIIILLILVL MFVVWMKRRD
760 770 780 790 800
KERQAKQLLI DPEDDVRDNI LKYDEEGGGE EDQDYDLSQL QQPDTVEPDA
810 820 830 840 850
IKPVGIRRLD ERPIHAEPQY PVRSAAPHPG DIGDFINEGL KAADNDPTAP
860 870 880 890 900
PYDSLLVFDY EGSGSTAGSL SSLNSSSSGG DQDYDYLNDW GPRFKKLADM

YGGGDD
Length:906
Mass (Da):99,686
Last modified:May 1, 1999 - v1
Checksum:i97D9937FD8D1F8B5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7G → A in BAA84919 (Ref. 2) Curated1
Sequence conflicti48T → D in BAA84919 (Ref. 2) Curated1
Sequence conflicti153A → R in BAA84919 (Ref. 2) Curated1
Sequence conflicti646A → V in BAA84919 (Ref. 2) Curated1
Sequence conflicti658N → K in BAA84919 (Ref. 2) Curated1
Sequence conflicti724T → A in BAA84919 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF097593 mRNA. Translation: AAC83818.1.
AB017695 mRNA. Translation: BAA84919.1.
RefSeqiNP_112623.1. NM_031333.1.
UniGeneiRn.23200.

Genome annotation databases

GeneIDi83501.
KEGGirno:83501.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF097593 mRNA. Translation: AAC83818.1.
AB017695 mRNA. Translation: BAA84919.1.
RefSeqiNP_112623.1. NM_031333.1.
UniGeneiRn.23200.

3D structure databases

ProteinModelPortaliQ9Z1Y3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249720. 1 interactor.
DIPiDIP-48902N.
STRINGi10116.ENSRNOP00000021170.

PTM databases

iPTMnetiQ9Z1Y3.
PhosphoSitePlusiQ9Z1Y3.
SwissPalmiQ9Z1Y3.
UniCarbKBiQ9Z1Y3.

Proteomic databases

PaxDbiQ9Z1Y3.
PRIDEiQ9Z1Y3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83501.
KEGGirno:83501.

Organism-specific databases

CTDi1000.
RGDi69280. Cdh2.

Phylogenomic databases

eggNOGiKOG3594. Eukaryota.
ENOG410XQHI. LUCA.
HOGENOMiHOG000231254.
HOVERGENiHBG106438.
InParanoidiQ9Z1Y3.
KOiK06736.
PhylomeDBiQ9Z1Y3.

Miscellaneous databases

PROiQ9Z1Y3.

Family and domain databases

Gene3Di2.60.40.60. 6 hits.
4.10.900.10. 1 hit.
InterProiIPR002126. Cadherin.
IPR015919. Cadherin-like.
IPR020894. Cadherin_CS.
IPR000233. Cadherin_cytoplasmic-dom.
IPR014868. Cadherin_pro_dom.
IPR027397. Catenin_binding_dom.
IPR030051. CDH2.
[Graphical view]
PANTHERiPTHR24027:SF79. PTHR24027:SF79. 1 hit.
PfamiPF00028. Cadherin. 5 hits.
PF01049. Cadherin_C. 1 hit.
PF08758. Cadherin_pro. 1 hit.
[Graphical view]
PRINTSiPR00205. CADHERIN.
SMARTiSM00112. CA. 5 hits.
SM01055. Cadherin_pro. 1 hit.
[Graphical view]
SUPFAMiSSF49313. SSF49313. 6 hits.
PROSITEiPS00232. CADHERIN_1. 3 hits.
PS50268. CADHERIN_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCADH2_RAT
AccessioniPrimary (citable) accession number: Q9Z1Y3
Secondary accession number(s): Q9R0T5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.