Q9Z1Y0 (RHOV_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rho-related GTP-binding protein RhoV

Alternative name(s):
Rho family GTPase Chp

Gene names

Name:

Rhov

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the control of the actin cytoskeleton via activation of the JNK pathway. Ref.1
Cofactor	Magnesium By similarity. UniProtKB Q7L0Q8
Subunit structure	Interacts with PAK2. Ref.1
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side. Endosome membrane; Lipid-anchor; Cytoplasmic side. Note: Treatment with TNF activates endosomal but not plasma membrane RHOV. Ref.3 Ref.4
Tissue specificity	Highly expressed in brain and testis and at lower levels in spleen and lung. Ref.1
Sequence similarities	Belongs to the small GTPase superfamily. Rho family. Ref.1

Ontologies

Keywords
Cellular component	Cell membrane Endosome Membrane
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Stk17b	Q91XS8	2	EBI-77480,EBI-77460

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	Rho-related GTP-binding protein RhoV		PRO_0000326440
Regions
	Nucleotide binding	38 – 45	8	GTP Ref.3
	Nucleotide binding	85 – 89	5	GTP By similarity UniProtKB Q7L0Q8
	Nucleotide binding	143 – 146	4	GTP By similarity UniProtKB P61586
Amino acid modifications
	Lipidation	234	1	S-palmitoyl cysteine Ref.3
Experimental info
	Mutagenesis	40	1	G → V: Constitutively GTP-bound and constitutively active. Ref.3
	Mutagenesis	56	1	Y → L: Increase in GDP/GTP cycling. Ref.3
	Mutagenesis	226 – 228	3	RCR → QCQ: Loss of subcellular location to plasma membrane, decreased association with endomembranes. Ref.4
	Mutagenesis	226	1	R → Q: Loss of subcellular location to plasma membrane. Ref.4
	Mutagenesis	227	1	C → S: No effect on subcellular location. Ref.3
	Mutagenesis	228	1	R → Q: Loss of subcellular location to plasma membrane. Ref.4
	Mutagenesis	229	1	W → Y: Loss of subcellular location to plasma and endosomal membrane. Ref.4
	Mutagenesis	230 – 231	2	KK → QQ: Loss of subcellular location to plasma membrane, decreased association with endomembranes. Ref.4
	Mutagenesis	230	1	K → Q: Loss of subcellular location to plasma membrane, decreased association with endomembranes. Ref.4
	Mutagenesis	231	1	K → Q: Loss of subcellular location to plasma membrane, decreased association with endomembranes. Ref.4
	Mutagenesis	234	1	C → S: Loss of subcellular location to plasma and endosomal membranes. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9Z1Y0 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 034416FA8CEA2425	FASTA	236	26,165
	10 20 30 40 50 60 MPPRELSEAE PPPLPASTPP PRRRSAPPEL GIKCVLVGDG AVGKSSLIVS YTCNGYPSRY 70 80 90 100 110 120 RPTALDTFSV QVLVDGAPVR IELWDTAGQE DFDRLRSLCY PDTDVFLACF SVVQPSSFQN 130 140 150 160 170 180 ITEKWLPEIR THNPQAPVLL VGTQADLRDD VNVLIQLDQG GREGPVPEPQ AQGLAEKIRA 190 200 210 220 230 CCYLECSALT QKNLKEVFDS AILSAIEHKA RLEKKLNAKG VRTLSRCRWK KFFCFV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Chp, a homologue of the GTPase Cdc42Hs, activates the JNK pathway and is implicated in reorganizing the actin cytoskeleton." Aronheim A., Broder Y.C., Cohen A., Fritsch A., Belisle B., Abo A. Curr. Biol. 8:1125-1128(1998) [PubMed: 9778532] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GTP-BINDING, TISSUE SPECIFICITY, INTERACTION WITH PAK2. Tissue: Pituitary.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Brown Norway. Tissue: Testis.
[3]	"Critical and distinct roles of amino- and carboxyl-terminal sequences in regulation of the biological activity of the Chp atypical Rho GTPase." Chenette E.J., Abo A., Der C.J. J. Biol. Chem. 280:13784-13792(2005) [PubMed: 15664990] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-40; CYS-227 AND CYS-234, PALMITOYLATION AT CYS-227.
[4]	"Multiple sequence elements facilitate Chp Rho GTPase subcellular location, membrane association, and transforming activity." Chenette E.J., Mitin N.Y., Der C.J. Mol. Biol. Cell 17:3108-3121(2006) [PubMed: 16641371] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-226; ARG-228; TRP-229; LYS-230 AND LYS-231.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF097887 mRNA. Translation: AAC69198.1. BC086990 mRNA. Translation: AAH86990.1.
IPI	IPI00209315.
RefSeq	NP_612551.1. NM_138542.2.
UniGene	Rn.30047.
3D structure databases
HSSP	HSSP built from PDB template 2C2H based on UniProtKB P60763.
ProteinModelPortal	Q9Z1Y0.
SMR	Q9Z1Y0. Positions 31-208.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Z1Y0. 2 interactions.
STRING	Q9Z1Y0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000018277; ENSRNOP00000018277; ENSRNOG00000013380.
GeneID	171581.
KEGG	rno:171581.
UCSC	NM_138542. rat.
Organism-specific databases
CTD	171177.
RGD	628824. Rhov.
Phylogenomic databases
eggNOG	maNOG09005.
GeneTree	ENSGT00560000076971.
HOVERGEN	HBG009351.
InParanoid	Q9Z1Y0.
OMA	TFSVQVL.
OrthoDB	EOG4255TJ.
PhylomeDB	Q9Z1Y0.
Gene expression databases
ArrayExpress	Q9Z1Y0.
Genevestigator	Q9Z1Y0.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003578. Small_GTPase_Rho. [Graphical view]
KO	K07866.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. Small_GTP. 1 hit.
PROSITE	PS51420. RHO. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	622616.

Entry information

Entry name

RHOV_RAT

Accession

Primary (citable) accession number: Q9Z1Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	May 1, 1999
Last modified:	November 16, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program