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Protein

Extended synaptotagmin-1

Gene

Esyt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport (By similarity). Binds calcium (via the C2 domains) and translocates to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels. Helps tether the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi336Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi337Calcium 1By similarity1
Metal bindingi337Calcium 2By similarity1
Metal bindingi349Calcium 2By similarity1
Metal bindingi396Calcium 1By similarity1
Metal bindingi396Calcium 2By similarity1
Metal bindingi398Calcium 1By similarity1
Metal bindingi398Calcium 2By similarity1
Metal bindingi398Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi400Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi402Calcium 3By similarity1
Metal bindingi403Calcium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processLipid transport, Transport
LigandCalcium, Lipid-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Extended synaptotagmin-1
Short name:
E-Syt1
Alternative name(s):
Membrane-bound C2 domain-containing protein
vp115
Gene namesi
Name:Esyt1
Synonyms:Fam62a, Mbc2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3053 Esyt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30CytoplasmicSequence analysisAdd BLAST30
Transmembranei31 – 51HelicalSequence analysisAdd BLAST21
Topological domaini52 – 54LumenalSequence analysis3
Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
Topological domaini76 – 1088CytoplasmicSequence analysisAdd BLAST1013

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002343471 – 1088Extended synaptotagmin-1Add BLAST1088

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei316Phosphoserine; by CDK5By similarity1
Modified residuei806N6-acetyllysineBy similarity1
Modified residuei809PhosphoserineBy similarity1
Modified residuei933PhosphoserineBy similarity1
Modified residuei947PhosphoserineBy similarity1
Modified residuei993PhosphotyrosineCombined sources1

Post-translational modificationi

Phosphorylated on Ser residues in insulin-treated adipocytes (in vitro); this promotes interaction with SLC2A4.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9Z1X1
PRIDEiQ9Z1X1

PTM databases

iPTMnetiQ9Z1X1
PhosphoSitePlusiQ9Z1X1

Expressioni

Tissue specificityi

Ubiquitously expressed with a higher expression in spleen and white adipose tissue.1 Publication

Interactioni

Subunit structurei

Interacts with ESYT2 and ESYT3. Interacts (phosphorylated form) with SLC2A4 (By similarity).By similarity

Protein-protein interaction databases

BioGridi248212, 2 interactors
IntActiQ9Z1X1, 1 interactor
STRINGi10116.ENSRNOP00000006119

Structurei

3D structure databases

ProteinModelPortaliQ9Z1X1
SMRiQ9Z1X1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini127 – 305SMP-LTDPROSITE-ProRule annotationAdd BLAST179
Domaini308 – 409C2 1PROSITE-ProRule annotationAdd BLAST102
Domaini457 – 550C2 2PROSITE-ProRule annotationAdd BLAST94
Domaini623 – 724C2 3PROSITE-ProRule annotationAdd BLAST102
Domaini774 – 866C2 4PROSITE-ProRule annotationAdd BLAST93
Domaini956 – 1061C2 5PROSITE-ProRule annotationAdd BLAST106

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1002 – 1009Required for phosphatidylinositol 4,5-bisphosphate-dependent location at the cell membraneBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi912 – 917Poly-Ser6

Domaini

Anchored to the endoplasmic reticulum membrane by a transmembrane hairpin structure; both N-terminus and C-terminus are cytoplasmic.By similarity
The C2 domains mediate lipid and calcium binding. The N-terminal C2 domain binds calcium ions and is important for calcium-dependent lipid binding and interaction with membranes. Two calcium ions are bound at a high-affinity site and a third calcium ion is bound with lower affinity. May bind up to four calcium ions. In contrast, the second C2 domain apparently does not bind calcium. The third C2 domain mediates interaction with membranes enriched in phosphatidylinositol 4,5-bisphosphate and is required for translocation to the cell membrane in response to increased cytosolic calcium levels (By similarity).By similarity
The SMP-LTD domain is a barrel-like domain that binds glycerophospholipids in its interior (By similarity).By similarity

Sequence similaritiesi

Belongs to the extended synaptotagmin family.Curated

Keywords - Domaini

Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1012 Eukaryota
COG5038 LUCA
HOGENOMiHOG000043080
HOVERGENiHBG055795
InParanoidiQ9Z1X1
PhylomeDBiQ9Z1X1
TreeFamiTF324255

Family and domain databases

CDDicd08391 C2A_C2C_Synaptotagmin_like, 2 hits
cd04050 C2B_Synaptotagmin-like, 2 hits
cd04030 C2C_KIAA1228, 1 hit
Gene3Di2.60.40.150, 5 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR037752 C2C_KIAA1228
IPR037733 Ext_Synaptotagmin_C2A
IPR037749 Ext_Synaptotagmin_C2B
IPR031468 SMP_LBD
PfamiView protein in Pfam
PF00168 C2, 5 hits
PF17047 SMP_LBD, 1 hit
PRINTSiPR00360 C2DOMAIN
SMARTiView protein in SMART
SM00239 C2, 5 hits
PROSITEiView protein in PROSITE
PS50004 C2, 4 hits
PS51847 SMP, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Z1X1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERSPEEGAG PEPSGQSPAT DSTRERDGGS GVPPAGPGAA SEALAVLTSF
60 70 80 90 100
GRRLLVLVPV YLAGAAGLSV GFVLFGLALY LGWRRVRDGK ERSLRAARQL
110 120 130 140 150
LDDEERITAE TLYMSHRELP AWVSFPDVEK AEWLNKIVVQ VWPFLGQYME
160 170 180 190 200
KLLAETVAPA VRGANPHLQT FTFTRVELGE KPVRIIGVKV HPSQRKDQIL
210 220 230 240 250
LDLNVSYVGD LQIDVEVKKY FCKAGVKGMQ LHGVLRVILE PLIGDLPIVG
260 270 280 290 300
AVSMFFIKRP TLDINWTGMT NLLDIPGLSS LSDTMIMDSI AAFLVLPNRL
310 320 330 340 350
LVPLVPDLQD VAQLRSPLPR GIIRIHLLAA RGLSSKDKYV KGLIEGKSDP
360 370 380 390 400
YALVRVGTQT FCSRVIDEEL NPHWGETYEV IVHEVPGQEI EVEVFDKDPD
410 420 430 440 450
KDDFLGRMKL DVGKVLQAGV LDNWYPLQGG QGQVHLRLEW LSLLPDAEKL
460 470 480 490 500
DQVLQWNRGI TSRPEPPSAA ILVVYLDRAQ DLPLKKGNKE PNPMVQLSVQ
510 520 530 540 550
DVTQESKATY STNCPVWEEA FRFFLQDPRS QELDVQVKDD SRALTLGALT
560 570 580 590 600
LPLARLLTAS ELTLDQWFQL SSSGPNSRLY MKLVMRILYL DSSEMRLPTE
610 620 630 640 650
PGAQDWDSES PETGSSVDAP PRPYHTTPNS HFGTENVLRI HVLEAQDLIA
660 670 680 690 700
KDRFLGGLVK GKSDPYVKLK VAGRSLRTHV VREDLNPRWN EVFEVIVTSI
710 720 730 740 750
PGQELDIEVF DKDLDKDDFL GRYKVGLTTV LNSGFLDEWL TLEDVPSGRL
760 770 780 790 800
HLRLERLSPR PTAAELEEVL QVNSLIQTQK SSELAAALLS VYLERSEDLP
810 820 830 840 850
LRKGTKPPSP YAILTVGETS HKTKTVSQTS APIWEESASF LIRKPHAESL
860 870 880 890 900
ELQVRGEGTG TLGSISLPLS ELLQEEQLCL DRWFALSGQG QVLMRVQLGI
910 920 930 940 950
LVSQHSGVEA HSHSSSSLNE EPEVLGDPTH TASPVLEVRH RLTHGDSPSE
960 970 980 990 1000
ALIGPLGQVK LTVWYHSDEQ KLISIIHSCR ALRQNGRDLP DPYVSVLLLP
1010 1020 1030 1040 1050
DKNRGTKRKT SQKKRTLNPE FNERFEWDLP LDGTLRRKLD VSVKSNSSFM
1060 1070 1080
SRERELLGKV QLDLAEIDLS QGAAQWYDLI DDRDKGGS
Length:1,088
Mass (Da):121,159
Last modified:May 1, 1999 - v1
Checksum:iE7E12D9737FFB89D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti373H → R in AAI01858 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099138 mRNA Translation: AAD10051.1
BC101857 mRNA Translation: AAI01858.1
RefSeqiNP_058945.2, NM_017249.2
UniGeneiRn.11636

Genome annotation databases

GeneIDi29579
KEGGirno:29579
UCSCiRGD:3053 rat

Similar proteinsi

Entry informationi

Entry nameiESYT1_RAT
AccessioniPrimary (citable) accession number: Q9Z1X1
Secondary accession number(s): Q3T1L3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health