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Protein

STE20/SPS1-related proline-alanine-rich protein kinase

Gene

Stk39

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a mediator of stress-activated signals. Mediates the inhibiton of SLC4A4, SLC26A6 as well as CFTR activities by the WNK scaffolds, probably through phosphorylation (PubMed:21317537, PubMed:23542070).2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041ATPPROSITE-ProRule annotation
Active sitei204 – 2041Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi81 – 899ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: MGI
  • protein serine/threonine kinase activity Source: ParkinsonsUK-UCL
  • receptor signaling protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • cellular hypotonic response Source: ParkinsonsUK-UCL
  • intracellular signal transduction Source: ParkinsonsUK-UCL
  • negative regulation of pancreatic juice secretion Source: UniProtKB
  • negative regulation of potassium ion transmembrane transport Source: ParkinsonsUK-UCL
  • negative regulation of potassium ion transmembrane transporter activity Source: ParkinsonsUK-UCL
  • negative regulation of protein phosphorylation Source: MGI
  • negative regulation of rubidium ion transmembrane transporter activity Source: ParkinsonsUK-UCL
  • negative regulation of rubidium ion transport Source: ParkinsonsUK-UCL
  • peptidyl-serine phosphorylation Source: MGI
  • peptidyl-threonine phosphorylation Source: ParkinsonsUK-UCL
  • positive regulation of potassium ion transport Source: Ensembl
  • regulation of blood pressure Source: MGI
  • regulation of inflammatory response Source: MGI
  • regulation of ion homeostasis Source: MGI
  • signal transduction by protein phosphorylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
STE20/SPS1-related proline-alanine-rich protein kinase (EC:2.7.11.1)
Short name:
Ste-20-related kinase
Alternative name(s):
Serine/threonine-protein kinase 39
Gene namesi
Name:Stk39
Synonyms:Spak
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1858416. Stk39.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • basolateral plasma membrane Source: MGI
  • cytoplasm Source: GO_Central
  • cytoskeleton Source: Ensembl
  • membrane Source: MGI
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556STE20/SPS1-related proline-alanine-rich protein kinasePRO_0000086723Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei321 – 3211Phosphoserine; by PKC/PRKCQBy similarity
Modified residuei361 – 3611N6-acetyllysineBy similarity
Modified residuei366 – 3661Phosphothreonine1 Publication
Modified residuei382 – 3821PhosphoserineBy similarity
Modified residuei383 – 3831Phosphoserine1 Publication
Modified residuei397 – 3971PhosphoserineCombined sources
Modified residuei405 – 4051PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated at Ser-321 by PRKCQ.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Z1W9.
MaxQBiQ9Z1W9.
PaxDbiQ9Z1W9.
PRIDEiQ9Z1W9.

PTM databases

iPTMnetiQ9Z1W9.
PhosphoSiteiQ9Z1W9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027030.
ExpressionAtlasiQ9Z1W9. baseline and differential.
GenevisibleiQ9Z1W9. MM.

Interactioni

Subunit structurei

The phosphorylated form forms a complex with WNK2.

Binary interactionsi

WithEntry#Exp.IntActNotes
Mapk14P478112EBI-444764,EBI-298727
Slc12a2P550123EBI-444764,EBI-621078
Slc12a6Q924N4-14EBI-444764,EBI-620992

Protein-protein interaction databases

BioGridi207310. 2 interactions.
DIPiDIP-31911N.
IntActiQ9Z1W9. 10 interactions.
STRINGi10090.ENSMUSP00000099776.

Structurei

Secondary structure

1
556
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi72 – 743Combined sources
Beta strandi75 – 839Combined sources
Beta strandi88 – 947Combined sources
Turni95 – 984Combined sources
Beta strandi99 – 1079Combined sources
Helixi108 – 1103Combined sources
Helixi114 – 12310Combined sources
Turni124 – 1274Combined sources
Beta strandi137 – 1426Combined sources
Beta strandi145 – 1517Combined sources
Beta strandi155 – 1573Combined sources
Helixi158 – 16710Combined sources
Turni168 – 1736Combined sources
Helixi178 – 19720Combined sources
Helixi207 – 2093Combined sources
Beta strandi210 – 2123Combined sources
Beta strandi218 – 2203Combined sources
Helixi224 – 2285Combined sources
Turni230 – 2323Combined sources
Helixi233 – 2364Combined sources
Helixi248 – 2503Combined sources
Helixi253 – 2608Combined sources
Helixi265 – 28016Combined sources
Turni284 – 2874Combined sources
Helixi290 – 29910Combined sources
Turni305 – 3084Combined sources
Helixi312 – 3154Combined sources
Helixi320 – 32910Combined sources
Turni334 – 3363Combined sources
Helixi340 – 3434Combined sources
Helixi347 – 3515Combined sources
Helixi355 – 3617Combined sources
Turni362 – 3643Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5D9HX-ray3.10A/B63-403[»]
5DBXX-ray2.50A/B63-390[»]
ProteinModelPortaliQ9Z1W9.
SMRiQ9Z1W9. Positions 14-427, 459-556.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini75 – 349275Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi372 – 3787Nuclear localization signalSequence analysis
Motifi399 – 4035Caspase cleavage related site

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi17 – 6549Ala/Pro-richAdd
BLAST
Compositional biasi22 – 3110Poly-Ala

Domaini

PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0582. Eukaryota.
ENOG410XSWS. LUCA.
GeneTreeiENSGT00840000129768.
HOGENOMiHOG000234204.
HOVERGENiHBG108518.
InParanoidiQ9Z1W9.
KOiK08835.
OMAiREASSCA.
OrthoDBiEOG091G0AKO.
PhylomeDBiQ9Z1W9.
TreeFamiTF105339.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR024678. Kinase_OSR1/WNK_CCT.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF12202. OSR1_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z1W9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPSGSPVH VQLSQQAAPV TAAAATAPAA ATSAPAPAPA PAPAASAAPA
60 70 80 90 100
PAPAAAPAPA PAAQAVGWPI CRDAYELQEV IGSGATAVVQ AALCKPRQER
110 120 130 140 150
VAIKRINLEK CQTSMDELLK EIQAMSQCSH PNVVTYYTSF VVKDELWLVM
160 170 180 190 200
KLLSGGSMLD IIKYIVNRGE HKNGVLEEAI IATILKEVLE GLDYLHRNGQ
210 220 230 240 250
IHRDLKAGNI LLGEDGSVQI ADFGVSAFLA TGGDVTRNKV RKTFVGTPCW
260 270 280 290 300
MAPEVMEQVR GYDFKADMWS FGITAIELAT GAAPYHKYPP MKVLMLTLQN
310 320 330 340 350
DPPTLETGVE DKEMMKKYGK SFRKLLSLCL QKDPSKRPTA AELLKCKFFQ
360 370 380 390 400
KAKNREYLIE KLLTRTPDIA QRAKKVRRVP GSSGHLHKTE DGDWEWSDDE
410 420 430 440 450
MDEKSEEGKA AASQEKSRRV KEENSEISVN AGGIPEQIQS LSVHDSQAQP
460 470 480 490 500
NANEDYREGP CAVNLVLRLR NSRKELNDIR FEFTPGRDTA DGVSQELFSA
510 520 530 540 550
GLVDGHDVVI VAANLQKIVD DPKALKTLTF KLASGCDGSE IPDEVKLIGF

AQLSVS
Length:556
Mass (Da):60,320
Last modified:May 1, 1999 - v1
Checksum:i66085A90554311D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099988 mRNA. Translation: AAC72237.1.
BC051964 mRNA. Translation: AAH51964.2.
BC064443 mRNA. Translation: AAH64443.1.
CCDSiCCDS16084.1.
RefSeqiNP_058562.1. NM_016866.2.
UniGeneiMm.198414.

Genome annotation databases

EnsembliENSMUST00000102715; ENSMUSP00000099776; ENSMUSG00000027030.
GeneIDi53416.
KEGGimmu:53416.
UCSCiuc008jxo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099988 mRNA. Translation: AAC72237.1.
BC051964 mRNA. Translation: AAH51964.2.
BC064443 mRNA. Translation: AAH64443.1.
CCDSiCCDS16084.1.
RefSeqiNP_058562.1. NM_016866.2.
UniGeneiMm.198414.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5D9HX-ray3.10A/B63-403[»]
5DBXX-ray2.50A/B63-390[»]
ProteinModelPortaliQ9Z1W9.
SMRiQ9Z1W9. Positions 14-427, 459-556.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207310. 2 interactions.
DIPiDIP-31911N.
IntActiQ9Z1W9. 10 interactions.
STRINGi10090.ENSMUSP00000099776.

PTM databases

iPTMnetiQ9Z1W9.
PhosphoSiteiQ9Z1W9.

Proteomic databases

EPDiQ9Z1W9.
MaxQBiQ9Z1W9.
PaxDbiQ9Z1W9.
PRIDEiQ9Z1W9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102715; ENSMUSP00000099776; ENSMUSG00000027030.
GeneIDi53416.
KEGGimmu:53416.
UCSCiuc008jxo.1. mouse.

Organism-specific databases

CTDi27347.
MGIiMGI:1858416. Stk39.

Phylogenomic databases

eggNOGiKOG0582. Eukaryota.
ENOG410XSWS. LUCA.
GeneTreeiENSGT00840000129768.
HOGENOMiHOG000234204.
HOVERGENiHBG108518.
InParanoidiQ9Z1W9.
KOiK08835.
OMAiREASSCA.
OrthoDBiEOG091G0AKO.
PhylomeDBiQ9Z1W9.
TreeFamiTF105339.

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.

Miscellaneous databases

ChiTaRSiStk39. mouse.
PROiQ9Z1W9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027030.
ExpressionAtlasiQ9Z1W9. baseline and differential.
GenevisibleiQ9Z1W9. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR024678. Kinase_OSR1/WNK_CCT.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF12202. OSR1_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTK39_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1W9
Secondary accession number(s): Q80W13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: September 7, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.