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Protein

STE20/SPS1-related proline-alanine-rich protein kinase

Gene

Stk39

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a mediator of stress-activated signals. Mediates the inhibiton of SLC4A4, SLC26A6 as well as CFTR activities by the WNK scaffolds, probably through phosphorylation (PubMed:21317537, PubMed:23542070).2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104ATPPROSITE-ProRule annotation1
Active sitei204Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi81 – 89ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: MGI
  • protein serine/threonine kinase activity Source: ParkinsonsUK-UCL
  • receptor signaling protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • cellular hypotonic response Source: ParkinsonsUK-UCL
  • intracellular signal transduction Source: ParkinsonsUK-UCL
  • maintenance of lens transparency Source: ParkinsonsUK-UCL
  • negative regulation of creatine transmembrane transporter activity Source: MGI
  • negative regulation of pancreatic juice secretion Source: UniProtKB
  • negative regulation of potassium ion transmembrane transport Source: ParkinsonsUK-UCL
  • negative regulation of potassium ion transmembrane transporter activity Source: ParkinsonsUK-UCL
  • negative regulation of protein phosphorylation Source: MGI
  • negative regulation of rubidium ion transmembrane transporter activity Source: ParkinsonsUK-UCL
  • negative regulation of rubidium ion transport Source: ParkinsonsUK-UCL
  • negative regulation of sodium ion transmembrane transporter activity Source: MGI
  • peptidyl-serine phosphorylation Source: MGI
  • peptidyl-threonine phosphorylation Source: ParkinsonsUK-UCL
  • positive regulation of potassium ion transport Source: Ensembl
  • regulation of blood pressure Source: MGI
  • regulation of inflammatory response Source: MGI
  • regulation of ion homeostasis Source: MGI
  • signal transduction by protein phosphorylation Source: MGI
  • signal transduction by trans-phosphorylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
STE20/SPS1-related proline-alanine-rich protein kinase (EC:2.7.11.1)
Short name:
Ste-20-related kinase
Alternative name(s):
Serine/threonine-protein kinase 39
Gene namesi
Name:Stk39
Synonyms:Spak
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1858416. Stk39.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • basolateral plasma membrane Source: MGI
  • cytoplasm Source: GO_Central
  • cytoskeleton Source: Ensembl
  • membrane Source: MGI
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867231 – 556STE20/SPS1-related proline-alanine-rich protein kinaseAdd BLAST556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei321Phosphoserine; by PKC/PRKCQBy similarity1
Modified residuei361N6-acetyllysineBy similarity1
Modified residuei366Phosphothreonine1 Publication1
Modified residuei382PhosphoserineBy similarity1
Modified residuei383Phosphoserine1 Publication1
Modified residuei397PhosphoserineCombined sources1
Modified residuei405PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Ser-321 by PRKCQ.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Z1W9.
MaxQBiQ9Z1W9.
PaxDbiQ9Z1W9.
PRIDEiQ9Z1W9.

PTM databases

iPTMnetiQ9Z1W9.
PhosphoSitePlusiQ9Z1W9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027030.
ExpressionAtlasiQ9Z1W9. baseline and differential.
GenevisibleiQ9Z1W9. MM.

Interactioni

Subunit structurei

The phosphorylated form forms a complex with WNK2.

Binary interactionsi

WithEntry#Exp.IntActNotes
Mapk14P478112EBI-444764,EBI-298727
Slc12a2P550123EBI-444764,EBI-621078
Slc12a6Q924N4-14EBI-444764,EBI-620992

Protein-protein interaction databases

BioGridi207310. 2 interactors.
DIPiDIP-31911N.
IntActiQ9Z1W9. 10 interactors.
STRINGi10090.ENSMUSP00000099776.

Structurei

Secondary structure

1556
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi72 – 74Combined sources3
Beta strandi75 – 83Combined sources9
Beta strandi88 – 94Combined sources7
Turni95 – 98Combined sources4
Beta strandi99 – 107Combined sources9
Helixi108 – 110Combined sources3
Helixi114 – 123Combined sources10
Turni124 – 127Combined sources4
Beta strandi137 – 142Combined sources6
Beta strandi145 – 151Combined sources7
Beta strandi155 – 157Combined sources3
Helixi158 – 167Combined sources10
Turni168 – 173Combined sources6
Helixi178 – 197Combined sources20
Helixi207 – 209Combined sources3
Beta strandi210 – 212Combined sources3
Beta strandi218 – 220Combined sources3
Helixi224 – 228Combined sources5
Turni230 – 232Combined sources3
Helixi233 – 236Combined sources4
Helixi248 – 250Combined sources3
Helixi253 – 260Combined sources8
Helixi265 – 280Combined sources16
Turni284 – 287Combined sources4
Helixi290 – 299Combined sources10
Turni305 – 308Combined sources4
Helixi312 – 315Combined sources4
Helixi320 – 329Combined sources10
Turni334 – 336Combined sources3
Helixi340 – 343Combined sources4
Helixi347 – 351Combined sources5
Helixi355 – 361Combined sources7
Turni362 – 364Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5D9HX-ray3.10A/B63-403[»]
5DBXX-ray2.50A/B63-390[»]
ProteinModelPortaliQ9Z1W9.
SMRiQ9Z1W9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini75 – 349Protein kinasePROSITE-ProRule annotationAdd BLAST275

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi372 – 378Nuclear localization signalSequence analysis7
Motifi399 – 403Caspase cleavage related site5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi17 – 65Ala/Pro-richAdd BLAST49
Compositional biasi22 – 31Poly-Ala10

Domaini

PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0582. Eukaryota.
ENOG410XSWS. LUCA.
GeneTreeiENSGT00860000133680.
HOGENOMiHOG000234204.
HOVERGENiHBG108518.
InParanoidiQ9Z1W9.
KOiK08835.
OMAiREASSCA.
OrthoDBiEOG091G0AKO.
PhylomeDBiQ9Z1W9.
TreeFamiTF105339.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR024678. Kinase_OSR1/WNK_CCT.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF12202. OSR1_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z1W9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPSGSPVH VQLSQQAAPV TAAAATAPAA ATSAPAPAPA PAPAASAAPA
60 70 80 90 100
PAPAAAPAPA PAAQAVGWPI CRDAYELQEV IGSGATAVVQ AALCKPRQER
110 120 130 140 150
VAIKRINLEK CQTSMDELLK EIQAMSQCSH PNVVTYYTSF VVKDELWLVM
160 170 180 190 200
KLLSGGSMLD IIKYIVNRGE HKNGVLEEAI IATILKEVLE GLDYLHRNGQ
210 220 230 240 250
IHRDLKAGNI LLGEDGSVQI ADFGVSAFLA TGGDVTRNKV RKTFVGTPCW
260 270 280 290 300
MAPEVMEQVR GYDFKADMWS FGITAIELAT GAAPYHKYPP MKVLMLTLQN
310 320 330 340 350
DPPTLETGVE DKEMMKKYGK SFRKLLSLCL QKDPSKRPTA AELLKCKFFQ
360 370 380 390 400
KAKNREYLIE KLLTRTPDIA QRAKKVRRVP GSSGHLHKTE DGDWEWSDDE
410 420 430 440 450
MDEKSEEGKA AASQEKSRRV KEENSEISVN AGGIPEQIQS LSVHDSQAQP
460 470 480 490 500
NANEDYREGP CAVNLVLRLR NSRKELNDIR FEFTPGRDTA DGVSQELFSA
510 520 530 540 550
GLVDGHDVVI VAANLQKIVD DPKALKTLTF KLASGCDGSE IPDEVKLIGF

AQLSVS
Length:556
Mass (Da):60,320
Last modified:May 1, 1999 - v1
Checksum:i66085A90554311D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099988 mRNA. Translation: AAC72237.1.
BC051964 mRNA. Translation: AAH51964.2.
BC064443 mRNA. Translation: AAH64443.1.
CCDSiCCDS16084.1.
RefSeqiNP_058562.1. NM_016866.2.
UniGeneiMm.198414.

Genome annotation databases

EnsembliENSMUST00000102715; ENSMUSP00000099776; ENSMUSG00000027030.
GeneIDi53416.
KEGGimmu:53416.
UCSCiuc008jxo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099988 mRNA. Translation: AAC72237.1.
BC051964 mRNA. Translation: AAH51964.2.
BC064443 mRNA. Translation: AAH64443.1.
CCDSiCCDS16084.1.
RefSeqiNP_058562.1. NM_016866.2.
UniGeneiMm.198414.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5D9HX-ray3.10A/B63-403[»]
5DBXX-ray2.50A/B63-390[»]
ProteinModelPortaliQ9Z1W9.
SMRiQ9Z1W9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207310. 2 interactors.
DIPiDIP-31911N.
IntActiQ9Z1W9. 10 interactors.
STRINGi10090.ENSMUSP00000099776.

PTM databases

iPTMnetiQ9Z1W9.
PhosphoSitePlusiQ9Z1W9.

Proteomic databases

EPDiQ9Z1W9.
MaxQBiQ9Z1W9.
PaxDbiQ9Z1W9.
PRIDEiQ9Z1W9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102715; ENSMUSP00000099776; ENSMUSG00000027030.
GeneIDi53416.
KEGGimmu:53416.
UCSCiuc008jxo.1. mouse.

Organism-specific databases

CTDi27347.
MGIiMGI:1858416. Stk39.

Phylogenomic databases

eggNOGiKOG0582. Eukaryota.
ENOG410XSWS. LUCA.
GeneTreeiENSGT00860000133680.
HOGENOMiHOG000234204.
HOVERGENiHBG108518.
InParanoidiQ9Z1W9.
KOiK08835.
OMAiREASSCA.
OrthoDBiEOG091G0AKO.
PhylomeDBiQ9Z1W9.
TreeFamiTF105339.

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.

Miscellaneous databases

ChiTaRSiStk39. mouse.
PROiQ9Z1W9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027030.
ExpressionAtlasiQ9Z1W9. baseline and differential.
GenevisibleiQ9Z1W9. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR024678. Kinase_OSR1/WNK_CCT.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF12202. OSR1_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTK39_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1W9
Secondary accession number(s): Q80W13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.