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Q9Z1W8

- AT12A_MOUSE

UniProt

Q9Z1W8 - AT12A_MOUSE

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Protein
Potassium-transporting ATPase alpha chain 2
Gene
Atp12a, Atp1al1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for potassium absorption in various tissues.

Catalytic activityi

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei387 – 38714-aspartylphosphate intermediate By similarity
Metal bindingi728 – 7281Magnesium By similarity
Metal bindingi732 – 7321Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrogen:potassium-exchanging ATPase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. protein binding Source: MGI

GO - Biological processi

  1. ATP biosynthetic process Source: InterPro
  2. potassium ion homeostasis Source: MGI
  3. regulation of pH Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-transporting ATPase alpha chain 2 (EC:3.6.3.10)
Alternative name(s):
Non-gastric H(+)/K(+) ATPase subunit alpha
Proton pump
Gene namesi
Name:Atp12a
Synonyms:Atp1al1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:1926943. Atp12a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9999Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei100 – 12021Helical; Reviewed prediction
Add
BLAST
Topological domaini121 – 14222Lumenal Reviewed prediction
Add
BLAST
Transmembranei143 – 16321Helical; Reviewed prediction
Add
BLAST
Topological domaini164 – 299136Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei300 – 31920Helical; Reviewed prediction
Add
BLAST
Topological domaini320 – 33112Lumenal Reviewed prediction
Add
BLAST
Transmembranei332 – 34918Helical; Reviewed prediction
Add
BLAST
Topological domaini350 – 783434Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei784 – 80320Helical; Reviewed prediction
Add
BLAST
Topological domaini804 – 81310Lumenal Reviewed prediction
Transmembranei814 – 83421Helical; Reviewed prediction
Add
BLAST
Topological domaini835 – 85420Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei855 – 87723Helical; Reviewed prediction
Add
BLAST
Topological domaini878 – 92952Lumenal Reviewed prediction
Add
BLAST
Transmembranei930 – 94920Helical; Reviewed prediction
Add
BLAST
Topological domaini950 – 96314Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei964 – 98219Helical; Reviewed prediction
Add
BLAST
Topological domaini983 – 99715Lumenal Reviewed prediction
Add
BLAST
Transmembranei998 – 101821Helical; Reviewed prediction
Add
BLAST
Topological domaini1019 – 103517Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10351035Potassium-transporting ATPase alpha chain 2
PRO_0000046261Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei954 – 9541Phosphoserine; by PKA By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z1W8.
PaxDbiQ9Z1W8.
PRIDEiQ9Z1W8.

PTM databases

PhosphoSiteiQ9Z1W8.

Expressioni

Tissue specificityi

Found in skin, kidney and distal colon.1 Publication

Gene expression databases

BgeeiQ9Z1W8.
GenevestigatoriQ9Z1W8.

Interactioni

Subunit structurei

Composed of two subunits: alpha (catalytic) and beta.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1W8.
SMRiQ9Z1W8. Positions 45-1035.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00560000076866.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiQ32MR8.
KOiK01544.
OMAiNLRVEWE.
OrthoDBiEOG7327N0.
TreeFamiTF312838.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z1W8-1 [UniParc]FASTAAdd to Basket

« Hide

MRRKTEIYSV ELNGTKDVEL ADQKDDKKFK GGKNKDSEPN KSQEEELKKE     50
LDLDDHRLSN TDLEQKYGTN IIQGLSSIRA AELLARDGPN ALTPPKQTPE 100
IIKFLKQMVG GFSILLWIGA ALCWIAYVIQ YVSSTASLDN VYLGAILVLV 150
VILTGIFAYY QEAKSTNIMA SFSKMIPQQA LVIRDAEKKI IPAEQLVVGD 200
VVEIKGGDQI PADIRLVFSQ GCKVDNSSLT GESEPQARST EFTHENPLET 250
KNIGFYSTTC LEGTATGIVI NTGDRTIIGR IASLASGVGS EKTPIAIEIE 300
HFVHIVAAVA VSVGVIFFIT AVCMKYYVLD AIIFLISIIV ANVPEGLLAT 350
VTVTLSLTAK RMAKKNCLVK NLEAVETLGS TSIICSDKTG TLTQNRMTVA 400
HLWFDNQIFV ADTSENQTKQ AFDQSSGTWA SLSKIITLCN RAEFRPGQES 450
VPIMKRVVVG DASETALLKF SEVILGDVMD IRKRNHKVAE IPFNSTNKFQ 500
LSIHETEDPN DKRFLMVMKG APERILEKCS TIMINGQEQP LDKSSADAFH 550
TAYMELGGLG ERVLGFCHLY LPADKFPQSY TFDVDSINFP TSNLCFVGLL 600
SMIDPPRSTV PDAVSKCRSA GIKVIMVTGD HPITAKAIAK SVGIISANNE 650
TVEDIAKRRN IAVEQVNKRE AKAAVVTGME LKDMTPEQLD ELLINYQEIV 700
FARTSPQQKL IIVEGCQRQD AVVAVTGDGV NDSPALKKAD IGIAMGIAGS 750
DAAKNAADMV LLDDNFASIV TGVEEGRLIF DNLKKTIAYT LTKNIAELCP 800
FLIYIVAGLP LPIGTITILF IDLGTDIIPS IALAYEKAES DIMNRKPRHK 850
KKDRLVNKQL AIYSYLHIGL MQALGGFLVY FTVYAQQGFW PTSLINLRVS 900
WETDDINDLE DSYGQEWTRY QRKYLEWTGS TAFFVAIMVQ QIADLIIRKT 950
RRNSIFQQGL FRNKVIWVGI ISQIIVALVL SYGLGSVTAL SFTMLRAQYW 1000
FVAVPHAILI WVYDEMRKLF IRLYPGSWWD KNMYY 1035
Length:1,035
Mass (Da):114,727
Last modified:July 27, 2011 - v3
Checksum:iBFB2C26D90305206
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti464 – 4641E → K in AAL68709. 1 Publication
Sequence conflicti975 – 9751I → M in AAD03421. 1 Publication
Sequence conflicti981 – 9811S → F in AAD03421. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF350499 Genomic DNA. Translation: AAL68709.1.
BC109011 mRNA. Translation: AAI09012.1.
AF100169 mRNA. Translation: AAD03421.1.
CCDSiCCDS27148.1.
RefSeqiNP_619593.2. NM_138652.2.
UniGeneiMm.273271.

Genome annotation databases

EnsembliENSMUST00000007340; ENSMUSP00000007340; ENSMUSG00000022229.
GeneIDi192113.
KEGGimmu:192113.
UCSCiuc007ubw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF350499 Genomic DNA. Translation: AAL68709.1 .
BC109011 mRNA. Translation: AAI09012.1 .
AF100169 mRNA. Translation: AAD03421.1 .
CCDSi CCDS27148.1.
RefSeqi NP_619593.2. NM_138652.2.
UniGenei Mm.273271.

3D structure databases

ProteinModelPortali Q9Z1W8.
SMRi Q9Z1W8. Positions 45-1035.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q9Z1W8.

Proteomic databases

MaxQBi Q9Z1W8.
PaxDbi Q9Z1W8.
PRIDEi Q9Z1W8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000007340 ; ENSMUSP00000007340 ; ENSMUSG00000022229 .
GeneIDi 192113.
KEGGi mmu:192113.
UCSCi uc007ubw.2. mouse.

Organism-specific databases

CTDi 479.
MGIi MGI:1926943. Atp12a.

Phylogenomic databases

eggNOGi COG0474.
GeneTreei ENSGT00560000076866.
HOGENOMi HOG000265622.
HOVERGENi HBG004298.
InParanoidi Q32MR8.
KOi K01544.
OMAi NLRVEWE.
OrthoDBi EOG7327N0.
TreeFami TF312838.

Miscellaneous databases

NextBioi 371111.
PROi Q9Z1W8.
SOURCEi Search...

Gene expression databases

Bgeei Q9Z1W8.
Genevestigatori Q9Z1W8.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure, promoter analysis, and chromosomal localization of the murine H(+)/K(+)-ATPase alpha 2 subunit gene."
    Zhang W., Kuncewicz T., Higham S.C., Kone B.C.
    J. Am. Soc. Nephrol. 12:2554-2564(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 488-498; 624-636; 710-718 AND 755-785, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Ouabain-sensitive H,K-ATPase: tissue-specific expression of the mammalian genes encoding the catalytic alpha subunit."
    Pestov N.B., Romanova L.G., Korneenko T.V., Egorov M.V., Kostina M.B., Sverdlov V.E., Askari A., Shakhparonov M.I., Modyanov N.N.
    FEBS Lett. 440:320-324(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 868-981, TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Skin.

Entry informationi

Entry nameiAT12A_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1W8
Secondary accession number(s): Q32MR8, Q8VHY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi