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Protein

Potassium-transporting ATPase alpha chain 2

Gene

Atp12a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for potassium absorption in various tissues.

Catalytic activityi

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3874-aspartylphosphate intermediateBy similarity1
Metal bindingi728MagnesiumBy similarity1
Metal bindingi732MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • ATP hydrolysis coupled proton transport Source: GO_Central
  • cellular potassium ion homeostasis Source: GO_Central
  • cellular sodium ion homeostasis Source: GO_Central
  • potassium ion homeostasis Source: MGI
  • potassium ion import Source: GO_Central
  • regulation of pH Source: MGI
  • sodium ion export from cell Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

ReactomeiR-MMU-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-transporting ATPase alpha chain 2 (EC:3.6.3.10)
Alternative name(s):
Non-gastric H(+)/K(+) ATPase subunit alpha
Proton pump
Gene namesi
Name:Atp12a
Synonyms:Atp1al1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1926943. Atp12a.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 99CytoplasmicSequence analysisAdd BLAST98
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Topological domaini121 – 142LumenalSequence analysisAdd BLAST22
Transmembranei143 – 163HelicalSequence analysisAdd BLAST21
Topological domaini164 – 299CytoplasmicSequence analysisAdd BLAST136
Transmembranei300 – 319HelicalSequence analysisAdd BLAST20
Topological domaini320 – 331LumenalSequence analysisAdd BLAST12
Transmembranei332 – 349HelicalSequence analysisAdd BLAST18
Topological domaini350 – 783CytoplasmicSequence analysisAdd BLAST434
Transmembranei784 – 803HelicalSequence analysisAdd BLAST20
Topological domaini804 – 813LumenalSequence analysis10
Transmembranei814 – 834HelicalSequence analysisAdd BLAST21
Topological domaini835 – 854CytoplasmicSequence analysisAdd BLAST20
Transmembranei855 – 877HelicalSequence analysisAdd BLAST23
Topological domaini878 – 929LumenalSequence analysisAdd BLAST52
Transmembranei930 – 949HelicalSequence analysisAdd BLAST20
Topological domaini950 – 963CytoplasmicSequence analysisAdd BLAST14
Transmembranei964 – 982HelicalSequence analysisAdd BLAST19
Topological domaini983 – 997LumenalSequence analysisAdd BLAST15
Transmembranei998 – 1018HelicalSequence analysisAdd BLAST21
Topological domaini1019 – 1035CytoplasmicSequence analysisAdd BLAST17

GO - Cellular componenti

  • basolateral plasma membrane Source: MGI
  • integral component of plasma membrane Source: GO_Central
  • intracellular membrane-bounded organelle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000462612 – 1035Potassium-transporting ATPase alpha chain 2Add BLAST1034

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphotyrosineBy similarity1
Modified residuei9PhosphoserineBy similarity1
Modified residuei59PhosphoserineBy similarity1
Modified residuei239PhosphoserineBy similarity1
Modified residuei286PhosphoserineBy similarity1
Modified residuei463PhosphoserineBy similarity1
Modified residuei601PhosphoserineBy similarity1
Modified residuei840PhosphoserineBy similarity1
Modified residuei954Phosphoserine; by PKABy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Z1W8.
MaxQBiQ9Z1W8.
PaxDbiQ9Z1W8.
PeptideAtlasiQ9Z1W8.
PRIDEiQ9Z1W8.

PTM databases

iPTMnetiQ9Z1W8.
PhosphoSitePlusiQ9Z1W8.

Expressioni

Tissue specificityi

Found in skin, kidney and distal colon.1 Publication

Gene expression databases

BgeeiENSMUSG00000022229.
GenevisibleiQ9Z1W8. MM.

Interactioni

Subunit structurei

Composed of two subunits: alpha (catalytic) and beta.

Protein-protein interaction databases

IntActiQ9Z1W8. 1 interactor.
STRINGi10090.ENSMUSP00000007340.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1W8.
SMRiQ9Z1W8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0203. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00850000132256.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiQ9Z1W8.
KOiK01544.
OMAiNQVFDQS.
OrthoDBiEOG091G01BB.
TreeFamiTF312838.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030318. Atp12a.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF282. PTHR24093:SF282. 2 hits.
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z1W8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRKTEIYSV ELNGTKDVEL ADQKDDKKFK GGKNKDSEPN KSQEEELKKE
60 70 80 90 100
LDLDDHRLSN TDLEQKYGTN IIQGLSSIRA AELLARDGPN ALTPPKQTPE
110 120 130 140 150
IIKFLKQMVG GFSILLWIGA ALCWIAYVIQ YVSSTASLDN VYLGAILVLV
160 170 180 190 200
VILTGIFAYY QEAKSTNIMA SFSKMIPQQA LVIRDAEKKI IPAEQLVVGD
210 220 230 240 250
VVEIKGGDQI PADIRLVFSQ GCKVDNSSLT GESEPQARST EFTHENPLET
260 270 280 290 300
KNIGFYSTTC LEGTATGIVI NTGDRTIIGR IASLASGVGS EKTPIAIEIE
310 320 330 340 350
HFVHIVAAVA VSVGVIFFIT AVCMKYYVLD AIIFLISIIV ANVPEGLLAT
360 370 380 390 400
VTVTLSLTAK RMAKKNCLVK NLEAVETLGS TSIICSDKTG TLTQNRMTVA
410 420 430 440 450
HLWFDNQIFV ADTSENQTKQ AFDQSSGTWA SLSKIITLCN RAEFRPGQES
460 470 480 490 500
VPIMKRVVVG DASETALLKF SEVILGDVMD IRKRNHKVAE IPFNSTNKFQ
510 520 530 540 550
LSIHETEDPN DKRFLMVMKG APERILEKCS TIMINGQEQP LDKSSADAFH
560 570 580 590 600
TAYMELGGLG ERVLGFCHLY LPADKFPQSY TFDVDSINFP TSNLCFVGLL
610 620 630 640 650
SMIDPPRSTV PDAVSKCRSA GIKVIMVTGD HPITAKAIAK SVGIISANNE
660 670 680 690 700
TVEDIAKRRN IAVEQVNKRE AKAAVVTGME LKDMTPEQLD ELLINYQEIV
710 720 730 740 750
FARTSPQQKL IIVEGCQRQD AVVAVTGDGV NDSPALKKAD IGIAMGIAGS
760 770 780 790 800
DAAKNAADMV LLDDNFASIV TGVEEGRLIF DNLKKTIAYT LTKNIAELCP
810 820 830 840 850
FLIYIVAGLP LPIGTITILF IDLGTDIIPS IALAYEKAES DIMNRKPRHK
860 870 880 890 900
KKDRLVNKQL AIYSYLHIGL MQALGGFLVY FTVYAQQGFW PTSLINLRVS
910 920 930 940 950
WETDDINDLE DSYGQEWTRY QRKYLEWTGS TAFFVAIMVQ QIADLIIRKT
960 970 980 990 1000
RRNSIFQQGL FRNKVIWVGI ISQIIVALVL SYGLGSVTAL SFTMLRAQYW
1010 1020 1030
FVAVPHAILI WVYDEMRKLF IRLYPGSWWD KNMYY
Length:1,035
Mass (Da):114,727
Last modified:July 27, 2011 - v3
Checksum:iBFB2C26D90305206
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti464E → K in AAL68709 (PubMed:11729223).Curated1
Sequence conflicti975I → M in AAD03421 (PubMed:9872395).Curated1
Sequence conflicti981S → F in AAD03421 (PubMed:9872395).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF350499 Genomic DNA. Translation: AAL68709.1.
BC109011 mRNA. Translation: AAI09012.1.
AF100169 mRNA. Translation: AAD03421.1.
CCDSiCCDS27148.1.
RefSeqiNP_619593.2. NM_138652.2.
UniGeneiMm.273271.

Genome annotation databases

EnsembliENSMUST00000007340; ENSMUSP00000007340; ENSMUSG00000022229.
GeneIDi192113.
KEGGimmu:192113.
UCSCiuc007ubw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF350499 Genomic DNA. Translation: AAL68709.1.
BC109011 mRNA. Translation: AAI09012.1.
AF100169 mRNA. Translation: AAD03421.1.
CCDSiCCDS27148.1.
RefSeqiNP_619593.2. NM_138652.2.
UniGeneiMm.273271.

3D structure databases

ProteinModelPortaliQ9Z1W8.
SMRiQ9Z1W8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Z1W8. 1 interactor.
STRINGi10090.ENSMUSP00000007340.

PTM databases

iPTMnetiQ9Z1W8.
PhosphoSitePlusiQ9Z1W8.

Proteomic databases

EPDiQ9Z1W8.
MaxQBiQ9Z1W8.
PaxDbiQ9Z1W8.
PeptideAtlasiQ9Z1W8.
PRIDEiQ9Z1W8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007340; ENSMUSP00000007340; ENSMUSG00000022229.
GeneIDi192113.
KEGGimmu:192113.
UCSCiuc007ubw.2. mouse.

Organism-specific databases

CTDi479.
MGIiMGI:1926943. Atp12a.

Phylogenomic databases

eggNOGiKOG0203. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00850000132256.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiQ9Z1W8.
KOiK01544.
OMAiNQVFDQS.
OrthoDBiEOG091G01BB.
TreeFamiTF312838.

Enzyme and pathway databases

ReactomeiR-MMU-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiQ9Z1W8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022229.
GenevisibleiQ9Z1W8. MM.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030318. Atp12a.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF282. PTHR24093:SF282. 2 hits.
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT12A_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1W8
Secondary accession number(s): Q32MR8, Q8VHY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.