Potassium-transporting ATPase alpha chain 2

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Reviewed, UniProtKB/Swiss-Prot Q9Z1W8 (AT12A_MOUSE)

Last modified February 9, 2010. Version 78. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Potassium-transporting ATPase alpha chain 2
    EC=3.6.3.10
Alternative name(s):
    Proton pump
    Non-gastric H(+)/K(+) ATPase subunit alpha

Gene names

Name:	Atp12a
Synonyms:	Atp1al1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	1035 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Catalyzes the hydrolysis of ATP coupled with the exchange of H⁺ and K⁺ ions across the plasma membrane. Responsible for potassium absorption in various tissues.
Catalytic activity	ATP + H₂O + H⁺(In) + K⁺(Out) = ADP + phosphate + H⁺(Out) + K⁺(In).
Subunit structure	Composed of two subunits: alpha (catalytic) and beta.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Found in skin, kidney and distal colon. Ref.3
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.

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Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro potassium ion homeostasis Inferred from mutant phenotype. Source: MGI potassium ion transport Inferred from electronic annotation. Source: UniProtKB-KW proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydrogen:potassium-exchanging ATPase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1035

1035

Potassium-transporting ATPase alpha chain 2

PRO_0000046261

Regions

Topological domain

1 – 99

Cytoplasmic Potential

Transmembrane

100 – 120

Potential

Topological domain

121 – 142

Lumenal Potential

Transmembrane

143 – 163

Potential

Topological domain

164 – 299

136

Cytoplasmic Potential

Transmembrane

300 – 319

Potential

Topological domain

320 – 331

Lumenal Potential

Transmembrane

332 – 349

Potential

Topological domain

350 – 783

434

Cytoplasmic Potential

Transmembrane

784 – 803

Potential

Topological domain

804 – 813

Lumenal Potential

Transmembrane

814 – 834

Potential

Topological domain

835 – 854

Cytoplasmic Potential

Transmembrane

855 – 877

Potential

Topological domain

878 – 929

Lumenal Potential

Transmembrane

930 – 949

Potential

Topological domain

950 – 963

Cytoplasmic Potential

Transmembrane

964 – 982

Potential

Topological domain

983 – 997

Lumenal Potential

Transmembrane

998 – 1018

Potential

Topological domain

1019 – 1035

Cytoplasmic Potential

Sites

Active site

387

4-aspartylphosphate intermediate By similarity

Metal binding

728

Magnesium By similarity

Metal binding

732

Magnesium By similarity

Amino acid modifications

Modified residue

954

Phosphoserine; by PKA By similarity

Experimental info

Sequence conflict

975

I → M in AAD03421. Ref.3

Sequence conflict

981

S → F in AAD03421. Ref.3

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9Z1W8-1 [UniParc].

Last modified August 16, 2004. Version 2.
Checksum: 6FF6E26F52BE1EC4
Show »

FASTA

1,035

114,726

        10         20         30         40         50         60 
MRRKTEIYSV ELNGTKDVEL ADQKDDKKFK GGKNKDSEPN KSQEEELKKE LDLDDHRLSN 

        70         80         90        100        110        120 
TDLEQKYGTN IIQGLSSIRA AELLARDGPN ALTPPKQTPE IIKFLKQMVG GFSILLWIGA 

       130        140        150        160        170        180 
ALCWIAYVIQ YVSSTASLDN VYLGAILVLV VILTGIFAYY QEAKSTNIMA SFSKMIPQQA 

       190        200        210        220        230        240 
LVIRDAEKKI IPAEQLVVGD VVEIKGGDQI PADIRLVFSQ GCKVDNSSLT GESEPQARST 

       250        260        270        280        290        300 
EFTHENPLET KNIGFYSTTC LEGTATGIVI NTGDRTIIGR IASLASGVGS EKTPIAIEIE 

       310        320        330        340        350        360 
HFVHIVAAVA VSVGVIFFIT AVCMKYYVLD AIIFLISIIV ANVPEGLLAT VTVTLSLTAK 

       370        380        390        400        410        420 
RMAKKNCLVK NLEAVETLGS TSIICSDKTG TLTQNRMTVA HLWFDNQIFV ADTSENQTKQ 

       430        440        450        460        470        480 
AFDQSSGTWA SLSKIITLCN RAEFRPGQES VPIMKRVVVG DASKTALLKF SEVILGDVMD 

       490        500        510        520        530        540 
IRKRNHKVAE IPFNSTNKFQ LSIHETEDPN DKRFLMVMKG APERILEKCS TIMINGQEQP 

       550        560        570        580        590        600 
LDKSSADAFH TAYMELGGLG ERVLGFCHLY LPADKFPQSY TFDVDSINFP TSNLCFVGLL 

       610        620        630        640        650        660 
SMIDPPRSTV PDAVSKCRSA GIKVIMVTGD HPITAKAIAK SVGIISANNE TVEDIAKRRN 

       670        680        690        700        710        720 
IAVEQVNKRE AKAAVVTGME LKDMTPEQLD ELLINYQEIV FARTSPQQKL IIVEGCQRQD 

       730        740        750        760        770        780 
AVVAVTGDGV NDSPALKKAD IGIAMGIAGS DAAKNAADMV LLDDNFASIV TGVEEGRLIF 

       790        800        810        820        830        840 
DNLKKTIAYT LTKNIAELCP FLIYIVAGLP LPIGTITILF IDLGTDIIPS IALAYEKAES 

       850        860        870        880        890        900 
DIMNRKPRHK KKDRLVNKQL AIYSYLHIGL MQALGGFLVY FTVYAQQGFW PTSLINLRVS 

       910        920        930        940        950        960 
WETDDINDLE DSYGQEWTRY QRKYLEWTGS TAFFVAIMVQ QIADLIIRKT RRNSIFQQGL 

       970        980        990       1000       1010       1020 
FRNKVIWVGI ISQIIVALVL SYGLGSVTAL SFTMLRAQYW FVAVPHAILI WVYDEMRKLF 

      1030 
IRLYPGSWWD KNMYY

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References

[1]	"Structure, promoter analysis, and chromosomal localization of the murine H(+)/K(+)-ATPase alpha 2 subunit gene." Zhang W., Kuncewicz T., Higham S.C., Kone B.C. J. Am. Soc. Nephrol. 12:2554-2564(2001) [PubMed: 11729223] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[2]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 488-498; 624-636; 710-718 AND 755-785, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[3]	"Ouabain-sensitive H,K-ATPase: tissue-specific expression of the mammalian genes encoding the catalytic alpha subunit." Pestov N.B., Romanova L.G., Korneenko T.V., Egorov M.V., Kostina M.B., Sverdlov V.E., Askari A., Shakhparonov M.I., Modyanov N.N. FEBS Lett. 440:320-324(1998) [PubMed: 9872395] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 868-981, TISSUE SPECIFICITY. Strain: C57BL/6. Tissue: Skin.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AF350499 Genomic DNA. Translation: AAL68709.1. AF100169 mRNA. Translation: AAD03421.1.
IPI	IPI00268145.
UniGene	Mm.273271
3D structure databases
SMR	Q9Z1W8. Positions 45-1035.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Z1W8.
PTM databases
PhosphoSite	Q9Z1W8.
Proteomic databases
PRIDE	Q9Z1W8.
Genome annotation databases
Ensembl	ENSMUST00000007340; ENSMUSP00000007340; ENSMUSG00000022229; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:1926943. Atp12a.
Phylogenomic databases
HOGENOM	HBG456486.
HOVERGEN	Q9Z1W8.
InParanoid	Q9Z1W8.
Enzyme and pathway databases
BRENDA	3.6.3.10. 244.
Gene expression databases
ArrayExpress	Q9Z1W8.
Bgee	Q9Z1W8.
Genevestigator	Q9Z1W8.
GermOnline	ENSMUSG00000022229. Mus musculus.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR005775. ATPase_P-typ_cation-ex_asu_euk. IPR006069. ATPase_P-typ_cation-exchng_asu. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00121. NAKATPASE.
SMART	SM00831. Cation_ATPase_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01106. ATPase-IIC_X-K. 1 hit. TIGR01494. ATPase_P-type. 4 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

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Entry information

Entry name

AT12A_MOUSE

Accession

Primary (citable) accession number: Q9Z1W8
Secondary accession number(s): Q8VHY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	August 16, 2004
Last modified:	February 9, 2010
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents