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Q9Z1W8

- AT12A_MOUSE

UniProt

Q9Z1W8 - AT12A_MOUSE

Protein

Potassium-transporting ATPase alpha chain 2

Gene

Atp12a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for potassium absorption in various tissues.

    Catalytic activityi

    ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei387 – 38714-aspartylphosphate intermediateBy similarity
    Metal bindingi728 – 7281MagnesiumBy similarity
    Metal bindingi732 – 7321MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydrogen:potassium-exchanging ATPase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: MGI

    GO - Biological processi

    1. ATP biosynthetic process Source: InterPro
    2. potassium ion homeostasis Source: MGI
    3. regulation of pH Source: MGI

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Hydrogen ion transport, Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium-transporting ATPase alpha chain 2 (EC:3.6.3.10)
    Alternative name(s):
    Non-gastric H(+)/K(+) ATPase subunit alpha
    Proton pump
    Gene namesi
    Name:Atp12a
    Synonyms:Atp1al1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:1926943. Atp12a.

    Subcellular locationi

    GO - Cellular componenti

    1. basolateral plasma membrane Source: MGI
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10351035Potassium-transporting ATPase alpha chain 2PRO_0000046261Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei954 – 9541Phosphoserine; by PKABy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9Z1W8.
    PaxDbiQ9Z1W8.
    PRIDEiQ9Z1W8.

    PTM databases

    PhosphoSiteiQ9Z1W8.

    Expressioni

    Tissue specificityi

    Found in skin, kidney and distal colon.1 Publication

    Gene expression databases

    BgeeiQ9Z1W8.
    GenevestigatoriQ9Z1W8.

    Interactioni

    Subunit structurei

    Composed of two subunits: alpha (catalytic) and beta.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z1W8.
    SMRiQ9Z1W8. Positions 45-1035.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9999CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini121 – 14222LumenalSequence AnalysisAdd
    BLAST
    Topological domaini164 – 299136CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini320 – 33112LumenalSequence AnalysisAdd
    BLAST
    Topological domaini350 – 783434CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini804 – 81310LumenalSequence Analysis
    Topological domaini835 – 85420CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini878 – 92952LumenalSequence AnalysisAdd
    BLAST
    Topological domaini950 – 96314CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini983 – 99715LumenalSequence AnalysisAdd
    BLAST
    Topological domaini1019 – 103517CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei100 – 12021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei143 – 16321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei300 – 31920HelicalSequence AnalysisAdd
    BLAST
    Transmembranei332 – 34918HelicalSequence AnalysisAdd
    BLAST
    Transmembranei784 – 80320HelicalSequence AnalysisAdd
    BLAST
    Transmembranei814 – 83421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei855 – 87723HelicalSequence AnalysisAdd
    BLAST
    Transmembranei930 – 94920HelicalSequence AnalysisAdd
    BLAST
    Transmembranei964 – 98219HelicalSequence AnalysisAdd
    BLAST
    Transmembranei998 – 101821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    GeneTreeiENSGT00560000076866.
    HOGENOMiHOG000265622.
    HOVERGENiHBG004298.
    InParanoidiQ32MR8.
    KOiK01544.
    OMAiNLRVEWE.
    OrthoDBiEOG7327N0.
    TreeFamiTF312838.

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z1W8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRRKTEIYSV ELNGTKDVEL ADQKDDKKFK GGKNKDSEPN KSQEEELKKE     50
    LDLDDHRLSN TDLEQKYGTN IIQGLSSIRA AELLARDGPN ALTPPKQTPE 100
    IIKFLKQMVG GFSILLWIGA ALCWIAYVIQ YVSSTASLDN VYLGAILVLV 150
    VILTGIFAYY QEAKSTNIMA SFSKMIPQQA LVIRDAEKKI IPAEQLVVGD 200
    VVEIKGGDQI PADIRLVFSQ GCKVDNSSLT GESEPQARST EFTHENPLET 250
    KNIGFYSTTC LEGTATGIVI NTGDRTIIGR IASLASGVGS EKTPIAIEIE 300
    HFVHIVAAVA VSVGVIFFIT AVCMKYYVLD AIIFLISIIV ANVPEGLLAT 350
    VTVTLSLTAK RMAKKNCLVK NLEAVETLGS TSIICSDKTG TLTQNRMTVA 400
    HLWFDNQIFV ADTSENQTKQ AFDQSSGTWA SLSKIITLCN RAEFRPGQES 450
    VPIMKRVVVG DASETALLKF SEVILGDVMD IRKRNHKVAE IPFNSTNKFQ 500
    LSIHETEDPN DKRFLMVMKG APERILEKCS TIMINGQEQP LDKSSADAFH 550
    TAYMELGGLG ERVLGFCHLY LPADKFPQSY TFDVDSINFP TSNLCFVGLL 600
    SMIDPPRSTV PDAVSKCRSA GIKVIMVTGD HPITAKAIAK SVGIISANNE 650
    TVEDIAKRRN IAVEQVNKRE AKAAVVTGME LKDMTPEQLD ELLINYQEIV 700
    FARTSPQQKL IIVEGCQRQD AVVAVTGDGV NDSPALKKAD IGIAMGIAGS 750
    DAAKNAADMV LLDDNFASIV TGVEEGRLIF DNLKKTIAYT LTKNIAELCP 800
    FLIYIVAGLP LPIGTITILF IDLGTDIIPS IALAYEKAES DIMNRKPRHK 850
    KKDRLVNKQL AIYSYLHIGL MQALGGFLVY FTVYAQQGFW PTSLINLRVS 900
    WETDDINDLE DSYGQEWTRY QRKYLEWTGS TAFFVAIMVQ QIADLIIRKT 950
    RRNSIFQQGL FRNKVIWVGI ISQIIVALVL SYGLGSVTAL SFTMLRAQYW 1000
    FVAVPHAILI WVYDEMRKLF IRLYPGSWWD KNMYY 1035
    Length:1,035
    Mass (Da):114,727
    Last modified:July 27, 2011 - v3
    Checksum:iBFB2C26D90305206
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti464 – 4641E → K in AAL68709. (PubMed:11729223)Curated
    Sequence conflicti975 – 9751I → M in AAD03421. (PubMed:9872395)Curated
    Sequence conflicti981 – 9811S → F in AAD03421. (PubMed:9872395)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF350499 Genomic DNA. Translation: AAL68709.1.
    BC109011 mRNA. Translation: AAI09012.1.
    AF100169 mRNA. Translation: AAD03421.1.
    CCDSiCCDS27148.1.
    RefSeqiNP_619593.2. NM_138652.2.
    UniGeneiMm.273271.

    Genome annotation databases

    EnsembliENSMUST00000007340; ENSMUSP00000007340; ENSMUSG00000022229.
    GeneIDi192113.
    KEGGimmu:192113.
    UCSCiuc007ubw.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF350499 Genomic DNA. Translation: AAL68709.1 .
    BC109011 mRNA. Translation: AAI09012.1 .
    AF100169 mRNA. Translation: AAD03421.1 .
    CCDSi CCDS27148.1.
    RefSeqi NP_619593.2. NM_138652.2.
    UniGenei Mm.273271.

    3D structure databases

    ProteinModelPortali Q9Z1W8.
    SMRi Q9Z1W8. Positions 45-1035.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9Z1W8.

    Proteomic databases

    MaxQBi Q9Z1W8.
    PaxDbi Q9Z1W8.
    PRIDEi Q9Z1W8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000007340 ; ENSMUSP00000007340 ; ENSMUSG00000022229 .
    GeneIDi 192113.
    KEGGi mmu:192113.
    UCSCi uc007ubw.2. mouse.

    Organism-specific databases

    CTDi 479.
    MGIi MGI:1926943. Atp12a.

    Phylogenomic databases

    eggNOGi COG0474.
    GeneTreei ENSGT00560000076866.
    HOGENOMi HOG000265622.
    HOVERGENi HBG004298.
    InParanoidi Q32MR8.
    KOi K01544.
    OMAi NLRVEWE.
    OrthoDBi EOG7327N0.
    TreeFami TF312838.

    Miscellaneous databases

    NextBioi 371111.
    PROi Q9Z1W8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9Z1W8.
    Genevestigatori Q9Z1W8.

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 1 hit.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure, promoter analysis, and chromosomal localization of the murine H(+)/K(+)-ATPase alpha 2 subunit gene."
      Zhang W., Kuncewicz T., Higham S.C., Kone B.C.
      J. Am. Soc. Nephrol. 12:2554-2564(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvJ.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 488-498; 624-636; 710-718 AND 755-785, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Ouabain-sensitive H,K-ATPase: tissue-specific expression of the mammalian genes encoding the catalytic alpha subunit."
      Pestov N.B., Romanova L.G., Korneenko T.V., Egorov M.V., Kostina M.B., Sverdlov V.E., Askari A., Shakhparonov M.I., Modyanov N.N.
      FEBS Lett. 440:320-324(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 868-981, TISSUE SPECIFICITY.
      Strain: C57BL/6.
      Tissue: Skin.

    Entry informationi

    Entry nameiAT12A_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z1W8
    Secondary accession number(s): Q32MR8, Q8VHY2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 116 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3