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Protein

Protein LYRIC

Gene

Mtdh

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Protein LYRIC
Alternative name(s):
Lysine-rich CEACAM1 co-isolated protein
Metadherin
Metastasis adhesion protein
Gene namesi
Name:Mtdh
Synonyms:Lyric
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi620992. Mtdh.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4949LumenalSequence analysisAdd
BLAST
Transmembranei50 – 7021HelicalSequence analysisAdd
BLAST
Topological domaini71 – 581511CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • bicellular tight junction Source: RGD
  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • intercellular canaliculus Source: RGD
  • nuclear body Source: Ensembl
  • nuclear membrane Source: UniProtKB-SubCell
  • nucleolus Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 581581Protein LYRICPRO_0000084535Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei142 – 1421PhosphothreonineBy similarity
Modified residuei179 – 1791PhosphoserineBy similarity
Modified residuei215 – 2151PhosphoserineBy similarity
Modified residuei250 – 2501PhosphoserineBy similarity
Modified residuei263 – 2631N6-acetyllysineBy similarity
Modified residuei297 – 2971PhosphoserineCombined sources
Modified residuei305 – 3051PhosphoserineBy similarity
Modified residuei310 – 3101PhosphoserineBy similarity
Modified residuei343 – 3431PhosphoserineBy similarity
Modified residuei368 – 3681PhosphoserineBy similarity
Modified residuei414 – 4141PhosphoserineBy similarity
Modified residuei425 – 4251PhosphoserineCombined sources
Modified residuei456 – 4561PhosphoserineCombined sources
Modified residuei477 – 4771PhosphoserineBy similarity
Modified residuei493 – 4931PhosphoserineBy similarity
Modified residuei495 – 4951PhosphoserineBy similarity
Modified residuei567 – 5671PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9Z1W6.
PeptideAtlasiQ9Z1W6.
PRIDEiQ9Z1W6.

PTM databases

iPTMnetiQ9Z1W6.
PhosphoSiteiQ9Z1W6.
SwissPalmiQ9Z1W6.

Expressioni

Tissue specificityi

Widely expressed, with highest levels in liver, kidney, prostate and small intestine. Not detected in endothelial cells.1 Publication

Gene expression databases

GenevisibleiQ9Z1W6. RN.

Interactioni

Subunit structurei

Interacts with BCCIP, CREBBP/CBP and RELA/p65.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9Z1W6. 1 interaction.
MINTiMINT-4577608.
STRINGi10116.ENSRNOP00000009989.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7171Activation of NF-kappa-BBy similarityAdd
BLAST
Regioni72 – 16897Interaction with BCCIPBy similarityAdd
BLAST
Regioni100 – 204105Interaction with RELABy similarityAdd
BLAST
Regioni380 – 44263Lung-homing for mammary tumorsBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi59 – 679Poly-Leu
Compositional biasi440 – 45011Poly-LysAdd
BLAST
Compositional biasi572 – 5765Poly-Lys

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIV9. Eukaryota.
ENOG410YF4D. LUCA.
GeneTreeiENSGT00390000004343.
HOGENOMiHOG000113417.
HOVERGENiHBG052379.
InParanoidiQ9Z1W6.
OMAiDWGRSWS.
OrthoDBiEOG7XWPPV.
PhylomeDBiQ9Z1W6.
TreeFamiTF331350.

Family and domain databases

InterProiIPR031402. LYRIC.
[Graphical view]
PfamiPF15686. LYRIC. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z1W6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAARSWQDEL AQQAEEGSAR LRELLSVGLG FLRTELGLDL GLEPKRYPSW
60 70 80 90 100
VILVGTGALG LLLLFLLGYG WAAACAGARK KRRSPPRKRE EVTPPTPAPE
110 120 130 140 150
DPAQLKNLRS EEQKKKNRKK LPEKPKPNGR TVEIPEDEVV RTPRSITAKQ
160 170 180 190 200
PPETDKKNEK SKKNKKKSKS DAKAVQNSSR HDGKEVDEGA WETKISHREK
210 220 230 240 250
RQQRKRDKVL TDSGSLDSTI PGIENTITVT TEQLTTASFP VGSKKNKGDS
260 270 280 290 300
HLNVQVSNFK SGKGDSTLQV SSGLNENITV NGGGWSEKSV KLSSQLSAGE
310 320 330 340 350
EKWNSVPPAS AGKRKTEQSA WTQDPGDTNA NGKDWGRNWS DRSIFSGIGS
360 370 380 390 400
TAEPVSQSTT SDYQWDGSRN QPHIDDEWSG LNGLSSADPS SDWNAPAEEW
410 420 430 440 450
GNWVDEDRAS LLKSQEPISN DQKDSDDDKE KGEGALPTGK SKKKKKKKKK
460 470 480 490 500
QGEDNSITQD TEDLEKDTRE ELPVNTSKAR PKQEKACSLK TMSTSDPVEV
510 520 530 540 550
LIKNSQPIKT LPPAISAEPS VTLSKGDSDK SSSQVPPMLQ DTDKPKSNAK
560 570 580
QNSVPPSQTK SETNWESPKQ IKKKKKARRE T
Length:581
Mass (Da):63,969
Last modified:October 1, 2001 - v2
Checksum:i9F86534238C3513C
GO
Isoform 2 (identifier: Q9Z1W6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     248-269: Missing.

Note: No experimental confirmation available.
Show »
Length:559
Mass (Da):61,669
Checksum:i6A2D604D37556D39
GO
Isoform 3 (identifier: Q9Z1W6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     349-382: Missing.

Note: No experimental confirmation available.
Show »
Length:547
Mass (Da):60,221
Checksum:i85792C6633BBDB9D
GO
Isoform 4 (identifier: Q9Z1W6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     248-269: Missing.
     349-382: Missing.

Note: No experimental confirmation available.
Show »
Length:525
Mass (Da):57,921
Checksum:iF8CB16CB4878988C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei248 – 26922Missing in isoform 2 and isoform 4. CuratedVSP_012227Add
BLAST
Alternative sequencei349 – 38234Missing in isoform 3 and isoform 4. CuratedVSP_012228Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100421 mRNA. Translation: AAC72405.2.
RefSeqiNP_596889.1. NM_133398.1. [Q9Z1W6-1]
UniGeneiRn.6864.

Genome annotation databases

EnsembliENSRNOT00000009989; ENSRNOP00000009989; ENSRNOG00000006870. [Q9Z1W6-1]
GeneIDi170910.
KEGGirno:170910.
UCSCiRGD:620992. rat. [Q9Z1W6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100421 mRNA. Translation: AAC72405.2.
RefSeqiNP_596889.1. NM_133398.1. [Q9Z1W6-1]
UniGeneiRn.6864.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Z1W6. 1 interaction.
MINTiMINT-4577608.
STRINGi10116.ENSRNOP00000009989.

PTM databases

iPTMnetiQ9Z1W6.
PhosphoSiteiQ9Z1W6.
SwissPalmiQ9Z1W6.

Proteomic databases

PaxDbiQ9Z1W6.
PeptideAtlasiQ9Z1W6.
PRIDEiQ9Z1W6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009989; ENSRNOP00000009989; ENSRNOG00000006870. [Q9Z1W6-1]
GeneIDi170910.
KEGGirno:170910.
UCSCiRGD:620992. rat. [Q9Z1W6-1]

Organism-specific databases

CTDi92140.
RGDi620992. Mtdh.

Phylogenomic databases

eggNOGiENOG410IIV9. Eukaryota.
ENOG410YF4D. LUCA.
GeneTreeiENSGT00390000004343.
HOGENOMiHOG000113417.
HOVERGENiHBG052379.
InParanoidiQ9Z1W6.
OMAiDWGRSWS.
OrthoDBiEOG7XWPPV.
PhylomeDBiQ9Z1W6.
TreeFamiTF331350.

Miscellaneous databases

PROiQ9Z1W6.

Gene expression databases

GenevisibleiQ9Z1W6. RN.

Family and domain databases

InterProiIPR031402. LYRIC.
[Graphical view]
PfamiPF15686. LYRIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel protein, LYRIC, localized to tight junctions of polarized epithelial cells."
    Britt D.E., Yang D.-F., Yang D.-Q., Flanagan D.L., Callanan H., Lim Y.-P., Lin S.-H., Hixson D.C.
    Exp. Cell Res. 300:134-148(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: Fischer.
    Tissue: Bile duct.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-297; SER-425; SER-456 AND SER-567, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLYRIC_RAT
AccessioniPrimary (citable) accession number: Q9Z1W6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: October 1, 2001
Last modified: July 6, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.