Reviewed,
UniProtKB/Swiss-Prot Q9Z1T6 (FYV1_MOUSE)
Last modified
February 9, 2010.
Version 88.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 1-phosphatidylinositol-3-phosphate 5-kinase Short name=Phosphatidylinositol-3-phosphate 5-kinase EC=2.7.1.150 Alternative name(s): FYVE finger-containing phosphoinositide kinase PIKfyve Phosphatidylinositol-3-phosphate 5-kinase type III Short name=Type III PIP kinase Short name=PIPkin-III p235 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 2097 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol-3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol-3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. The product of the reaction it catalyzes functions as an important regulator of vacuole homeostasis perhaps by controlling membrane flux to and/or from the vacuole. Ref.7 |
| Catalytic activity | ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate. Ref.4 |
| Subunit structure | Component of the PI(3,5)P2 regulatory complex/PAS complex, at least composed of PIKFYVE, FIG4 and VAC14. VAC14 nucleates the assembly of the complex and serves as a scaffold. |
| Subcellular location | Cytoplasmic vesicle membrane; Peripheral membrane protein. Endosome membrane By similarity. Note: Associated with vesicle structures. Displays a peripheral vesicular punctate pattern. Mainly associated with membranes of the late endocytic pathway By similarity. Ref.7 |
| Tissue specificity | Ubiquitous. Ref.6 |
| Sequence similarities | Contains 1 DEP domain. Contains 1 FYVE-type zinc finger. Contains 1 PIPK domain. |
| Sequence caution | The sequence AAD10191.1 differs from that shown. Reason: Frameshift at several positions. |
Ontologies
Alternative products
| This entry describes 3 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q9Z1T6-2) Also known as: p235S; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q9Z1T6-1) Also known as: p235L; The sequence of this isoform differs from the canonical sequence as follows: 107-107: L → LENTLPHPQEST 490-545: Missing. | ||||||
| Isoform 3 (identifier: Q9Z1T6-3) The sequence of this isoform differs from the canonical sequence as follows: 490-497: NSASPSKR → SKFGFLML 498-2097: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 2097 | 2096 | 1-phosphatidylinositol-3-phosphate 5-kinase | PRO_0000185453 | |||||
Regions | |||||||||
| Domain | 365 – 440 | 76 | DEP | ||||||
| Domain | 1757 – 2083 | 327 | PIPK | ||||||
| Zinc finger | 158 – 218 | 61 | FYVE-type | ||||||
| Region | 1841 – 2097 | 257 | Catalytic By similarity | ||||||
| Compositional bias | 1222 – 1225 | 4 | Poly-Ser | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylalanine By similarity | ||||||
| Modified residue | 8 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 20 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 34 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 46 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 73 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 76 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 88 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 219 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 223 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 299 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 307 | 1 | Phosphoserine Ref.5 | ||||||
| Modified residue | 318 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 323 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 329 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 352 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 449 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 452 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 454 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 457 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 475 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 477 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 491 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 493 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1154 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 1525 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1543 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1548 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1713 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1753 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1771 | 1 | Phosphotyrosine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 107 | 1 | L → LENTLPHPQEST in isoform 2. | VSP_034953 | |||||
| Alternative sequence | 490 – 545 | 56 | Missing in isoform 2. | VSP_034954 | |||||
| Alternative sequence | 490 – 497 | 8 | NSASPSKR → SKFGFLML in isoform 3. | VSP_034955 | |||||
| Alternative sequence | 498 – 2097 | 1600 | Missing in isoform 3. | VSP_034956 | |||||
Experimental info | |||||||||
| Sequence conflict | 546 | 1 | E → K in AAD10191. Ref.1 | ||||||
| Sequence conflict | 944 | 1 | Q → K in AAD10191. Ref.1 | ||||||
| Sequence conflict | 994 – 995 | 2 | QP → HR in AAD10191. Ref.1 | ||||||
| Sequence conflict | 1107 | 1 | R → T in BAB30626. Ref.3 | ||||||
| Sequence conflict | 1141 | 1 | L → V in AAD10191. Ref.1 | ||||||
| Sequence conflict | 1264 | 1 | S → Y in AAD10191. Ref.1 | ||||||
| Sequence conflict | 1996 | 1 | V → E in AAD10191. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning, characterization, and expression of a novel Zn2+-binding FYVE finger-containing phosphoinositide kinase in insulin-sensitive cells." Shisheva A., Sbrissa D., Ikonomov O. Mol. Cell. Biol. 19:623-634(1999) [PubMed: 9858586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), CHARACTERIZATION. Tissue: Adipose tissue. |
| [2] | "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed: 15368895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Pancreatic islet. |
| [3] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: C57BL/6J. Tissue: Cerebellum, Ovary, Spleen and Uterus. |
| [4] | "Complementation analysis in PtdInsP kinase-deficient yeast mutants demonstrates that Schizosaccharomyces pombe and murine Fab1p homologues are phosphatidylinositol 3-phosphate 5-kinases." McEwen R.K., Dove S.K., Cooke F.T., Painter G.F., Holmes A.B., Shisheva A., Ohya Y., Parker P.J., Michell R.H. J. Biol. Chem. 274:33905-33912(1999) [PubMed: 10567352] [Abstract] Cited for: CATALYTIC ACTIVITY. |
| [5] | "Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-307, MASS SPECTROMETRY. Tissue: Liver. |
| [6] | "Loss of Vac14, a regulator of the signaling lipid phosphatidylinositol 3,5-bisphosphate, results in neurodegeneration in mice." Zhang Y., Zolov S.N., Chow C.Y., Slutsky S.G., Richardson S.C., Piper R.C., Yang B., Nau J.J., Westrick R.J., Morrison S.J., Meisler M.H., Weisman L.S. Proc. Natl. Acad. Sci. U.S.A. 104:17518-17523(2007) [PubMed: 17956977] [Abstract] Cited for: TISSUE SPECIFICITY. |
| [7] | "VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse." Jin N., Chow C.Y., Liu L., Zolov S.N., Bronson R., Davisson M., Petersen J.L., Zhang Y., Park S., Duex J.E., Goldowitz D., Meisler M.H., Weisman L.S. EMBO J. 27:3221-3234(2008) [PubMed: 19037259] [Abstract] Cited for: IDENTIFICATION IN THE PI(3,5)P2 REGULATORY COMPLEX, FUNCTION, SUBCELLULAR LOCATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF102777 mRNA. Translation: AAD10191.1. Frameshift. AK173077 mRNA. Translation: BAD32355.1. Different initiation. AK017186 mRNA. Translation: BAB30626.3. Different initiation. AK139116 mRNA. Translation: BAE23894.1. AK165350 mRNA. Translation: BAE38145.1. |
| IPI | IPI00130449. IPI00230618. IPI00874822. |
| PIR | T18290. |
| RefSeq | NP_035216.2. |
| UniGene | Mm.38370 |
3D structure databases | |
| SMR | Q9Z1T6. Positions 145-216, 355-437, 629-863, 1819-2083. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q9Z1T6. |
PTM databases | |
| PhosphoSite | Q9Z1T6. |
2-D gel databases | |
| SWISS-2DPAGE | Q9Z1T6. |
Proteomic databases | |
| PRIDE | Q9Z1T6. |
Genome annotation databases | |
| Ensembl | ENSMUST00000097707; ENSMUSP00000095314; ENSMUSG00000025949; Mus musculus. [Genome view] ENSMUST00000114042; ENSMUSP00000109676; ENSMUSG00000025949; Mus musculus. [Genome view] |
| GeneID | 18711. |
| KEGG | mmu:18711. |
Organism-specific databases | |
| CTD | 18711. |
| MGI | MGI:1335106. Pikfyve. |
| Rouge | Search... |
Phylogenomic databases | |
| eggNOG | roNOG08866. |
| HOVERGEN | Q9Z1T6. |
Enzyme and pathway databases | |
| BRENDA | 2.7.1.68. 244. |
Gene expression databases | |
| Genevestigator | Q9Z1T6. |
| GermOnline | ENSMUSG00000025949. Mus musculus. |
Family and domain databases | |
| InterPro | IPR002423. Cpn60/TCP-1. IPR000591. DEP_dom. IPR002498. PInositol-4-P-5-kinase_core. IPR016034. PInositol-4P-5-kinase_core_sub. IPR011991. WHTH_trsnscrt_rep_DNA-bd. IPR000306. Znf_FYVE. IPR017455. Znf_FYVE-rel. IPR011011. Znf_FYVE_PHD. [Graphical view] |
| Gene3D | G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit. |
| PANTHER | PTHR11353. Cpn60/TCP-1. 1 hit. |
| Pfam | PF00118. Cpn60_TCP1. 1 hit. PF00610. DEP. 1 hit. PF01363. FYVE. 1 hit. PF01504. PIP5K. 1 hit. [Graphical view] |
| SMART | SM00049. DEP. 1 hit. SM00064. FYVE. 1 hit. SM00330. PIPKc. 1 hit. [Graphical view] |
| PROSITE | PS50186. DEP. 1 hit. PS51455. PIPK. 1 hit. PS50178. ZF_FYVE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 294793. |
| SOURCE | Search... |
Entry information
| Entry name | FYV1_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q9Z1T6 Secondary accession number(s): Q3TNE4 Q9CU94 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


