Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

1-phosphatidylinositol 3-phosphate 5-kinase

Gene

Pikfyve

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. The product of the reaction it catalyzes functions as an important regulator of vacuole homeostasis perhaps by controlling membrane flux to and/or from the vacuole.1 Publication

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri158 – 218FYVE-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

GO - Biological processi

  • intracellular signal transduction Source: UniProtKB
  • myelin assembly Source: MGI
  • protein localization to nucleus Source: MGI
  • regulation of autophagosome assembly Source: MGI
  • retrograde transport, endosome to Golgi Source: MGI

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.150 3474
ReactomeiR-MMU-1660514 Synthesis of PIPs at the Golgi membrane
R-MMU-1660516 Synthesis of PIPs at the early endosome membrane
R-MMU-1660517 Synthesis of PIPs at the late endosome membrane

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 3-phosphate 5-kinase (EC:2.7.1.150)
Short name:
Phosphatidylinositol 3-phosphate 5-kinase
Alternative name(s):
FYVE finger-containing phosphoinositide kinase
PIKfyve
Phosphatidylinositol 3-phosphate 5-kinase type III
Short name:
PIPkin-III
Short name:
Type III PIP kinase
p235
Gene namesi
Name:Pikfyve
Synonyms:Kiaa0981, Pip5k3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1335106 Pikfyve

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176842

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001854532 – 20971-phosphatidylinositol 3-phosphate 5-kinaseAdd BLAST2096

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei48PhosphoserineBy similarity1
Modified residuei88PhosphoserineBy similarity1
Modified residuei299PhosphoserineCombined sources1
Modified residuei307PhosphoserineBy similarity1
Modified residuei312PhosphoserineCombined sources1
Modified residuei318Phosphoserine; by PKB/AKT1 or PKB/AKT2By similarity1
Modified residuei329PhosphoserineBy similarity1
Modified residuei475PhosphoserineCombined sources1
Modified residuei1543PhosphoserineBy similarity1
Modified residuei1548PhosphoserineBy similarity1
Modified residuei1753PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated in response to insulin at Ser-318 in a protein kinase B (PKB)-dependent manner.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9Z1T6
PaxDbiQ9Z1T6
PeptideAtlasiQ9Z1T6
PRIDEiQ9Z1T6

2D gel databases

SWISS-2DPAGEiQ9Z1T6

PTM databases

iPTMnetiQ9Z1T6
PhosphoSitePlusiQ9Z1T6

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSMUSG00000025949
ExpressionAtlasiQ9Z1T6 baseline and differential
GenevisibleiQ9Z1T6 MM

Interactioni

Subunit structurei

Component of the PI(3,5)P2 regulatory complex/PAS complex, at least composed of PIKFYVE, FIG4 and VAC14. VAC14 nucleates the assembly of the complex and serves as a scaffold.1 Publication

Protein-protein interaction databases

BioGridi202165, 2 interactors
CORUMiQ9Z1T6
IntActiQ9Z1T6, 1 interactor
STRINGi10090.ENSMUSP00000079926

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini365 – 440DEPPROSITE-ProRule annotationAdd BLAST76
Domaini1757 – 2083PIPKPROSITE-ProRule annotationAdd BLAST327

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1841 – 2097CatalyticBy similarityAdd BLAST257

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1222 – 1225Poly-Ser4

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri158 – 218FYVE-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IMP3 Eukaryota
KOG0230 Eukaryota
COG0459 LUCA
COG5253 LUCA
GeneTreeiENSGT00840000129741
HOGENOMiHOG000059273
HOVERGENiHBG005775
InParanoidiQ9Z1T6
KOiK00921
OMAiWKKICHH
OrthoDBiEOG091G00JB
TreeFamiTF321717

Family and domain databases

CDDicd04448 DEP_PIKfyve, 1 hit
Gene3Di1.10.10.10, 1 hit
3.30.40.10, 1 hit
3.30.800.10, 1 hit
3.50.7.10, 1 hit
InterProiView protein in InterPro
IPR002423 Cpn60/TCP-1
IPR000591 DEP_dom
IPR027409 GroEL-like_apical_dom_sf
IPR037378 PIKfyve_DEP
IPR002498 PInositol-4-P-5-kinase_core
IPR027484 PInositol-4-P-5-kinase_N
IPR027410 TCP-1-like_intermed_sf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
IPR000306 Znf_FYVE
IPR017455 Znf_FYVE-rel
IPR011011 Znf_FYVE_PHD
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF00118 Cpn60_TCP1, 1 hit
PF00610 DEP, 1 hit
PF01363 FYVE, 1 hit
PF01504 PIP5K, 2 hits
SMARTiView protein in SMART
SM00049 DEP, 1 hit
SM00064 FYVE, 1 hit
SM00330 PIPKc, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF52029 SSF52029, 1 hit
SSF54849 SSF54849, 2 hits
SSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS50186 DEP, 1 hit
PS51455 PIPK, 1 hit
PS50178 ZF_FYVE, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z1T6-2) [UniParc]FASTAAdd to basket
Also known as: p235S

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATDDKSSPT LDSANDLPRS PASPSHLTHF KPLTPDQDEP PFKSAYSSFV
60 70 80 90 100
NLFRFNKERG EGGQGEQQSP SSSWASPQIP SRTQSVRSPV PYKKQLNEEL
110 120 130 140 150
HRRSSVLDSR RKAEPACGGH DPRTAVQLRS LSTVLKRLKE IMEGKSQDSD
160 170 180 190 200
LKQYWMPDSQ CKECYDCSEK FTTFRRRHHC RLCGQIFCSR CCNQEIPGKF
210 220 230 240 250
MGYTGDLRAC TYCRKIALSY AHSTDSNSIG EDLNALSDST CSVSILDPSE
260 270 280 290 300
PRTPVGSRKA SRNIFLEDDL AWQSLIHPDS SNSALSTRLV SVQEDAGKSP
310 320 330 340 350
ARNRSASITN LSLDRSGSPM VPSYETSVSP QANRNYIRTE TTEDERKILL
360 370 380 390 400
DSAQLKDLWK KICHHTSGME FQDHRYWLRT HPNCIVGKEL VNWLIRNGHI
410 420 430 440 450
ATRAQAIAIG QAMVDGRWLD CVSHHDQLFR DEYALYRPLQ STEFSETPSP
460 470 480 490 500
DSDSVNSVEG HSEPSWFKDI KFDDSDTEQI AEEGDDNLAN SASPSKRTSV
510 520 530 540 550
SSFQSTVDSD SAASISLNVE LDNVNFHIKK PSKYPHVPPH PADQKEYLVS
560 570 580 590 600
DTGGQQLSIS DAFIKESLFN RRVEEKSKEL PFTPLGWHHN NLELLREENE
610 620 630 640 650
EKQAMERLLS ANHNHMMALL QQLLQNESLS SSWRDIIVSL VCQVVQTVRP
660 670 680 690 700
DVKHQDDDMD IRQFVHIKKI PGGKKFDSVV VNGFVCTKNI AHKKMNSCIK
710 720 730 740 750
NPKILLLKCS IEYLYREETK FTCIDPIVLQ EREFLKNYVQ RIVDVRPTLV
760 770 780 790 800
LVEKTVSRIA QDMLLEHGIT LVINVKSQVL ERISRMTQGD LVVSMDQLLT
810 820 830 840 850
KPHLGTCHKF YMQIFQLPNE QTKTLMFFEG CPQHLGCTIK LRGGSDYELA
860 870 880 890 900
RVKEILIFMI CVAYHSQLEI SFLMDEFAMP PTLMQSPSFH LLTEGRGEEG
910 920 930 940 950
ASQEQVSGSS LPQDPECPRE ALSSEDSTLL ESRTVLEKGE LDNKSIPQAV
960 970 980 990 1000
ASLKHQDYTT PTCPAGIPCA LFALVPESLL PLHMDQQDAV GNEQPETSQQ
1010 1020 1030 1040 1050
TDEQQDPKSQ MKAFRDPLQD DTGMYVTEEV TSSEDQRKTY ALTFKQELKD
1060 1070 1080 1090 1100
VILCISPVIT FREPFLLTEK GMRCSTRDYF PEQIYWSPLL NKEVKEMESR
1110 1120 1130 1140 1150
RKKQLLRDLS GLQGMNGSVQ AKSIQVLPSH ELVSTRIAEH LGDSQTLGRM
1160 1170 1180 1190 1200
LADYRARGGR IQSKHLDPFV HSKDASCTSG GKSGNKTESD EERGLIPSDV
1210 1220 1230 1240 1250
IWPTKVDCLN PANHQRLCVL FSSSSAQSSN APSACVSPWI VTMEFYGKND
1260 1270 1280 1290 1300
LTLGIFLERY CFRSSYQCPS MFCDTPMVHH IRRFVHGQGC VQIILKELDS
1310 1320 1330 1340 1350
PVPGYQHTIL TYSWCRICKQ VTPVVALSNE SWSMSFAKYL ELRFYGHQYT
1360 1370 1380 1390 1400
RRANAEPCGH SIHHDYHQYF SYNQMVASFS YSPIRLLEVC VPLPKIFIKR
1410 1420 1430 1440 1450
QAPLKVSLLQ DLKDFFQKVS QVYLAVDERL ASLKTDTFSK TREEKMEDIF
1460 1470 1480 1490 1500
AQKEMEEGEF KNWTEKMQAR LMSSSVDTPQ QLQSIFESLI AKKQSLCEVL
1510 1520 1530 1540 1550
QAWNSRLQDL FQQEKGRKRP SVPPSPGRLR QGEESKINAM DTSPRNISPG
1560 1570 1580 1590 1600
LHNGEKEDRF LTTLSSQSST SSTHLQLPTP PEALAEQVVG GPTDLDSASG
1610 1620 1630 1640 1650
SEDVFDGHLL GSTDSQVKEK STMKAIFANL LPGNSYNPIP FPFDPDKHYL
1660 1670 1680 1690 1700
MYEHERVPIA VCEKEPSSII AFALSCKEYR NALEELSKAT LRNSAEEGLP
1710 1720 1730 1740 1750
ANSALDNRPK SSSPIRLPEI SGGQTNRTVE AEPQPTKKAS GMLSFFRGTA
1760 1770 1780 1790 1800
GKSPDLSSQK RETLRGADSA YYQVGQAGKE GLESQGLEPQ DEVDGGDTQK
1810 1820 1830 1840 1850
KQLTNPHVEL QFSDANAKFY CRLYYAGEFH KMREVILGSS EEEFIRSLSH
1860 1870 1880 1890 1900
SSPWQARGGK SGAAFYATED DRFILKQMPR LEVQSFLDFA PHYFNYITNA
1910 1920 1930 1940 1950
VQQKRPTALA KILGVYRIGY KNSQNNTEKK LDLLVMENLF YGRKMAQVFD
1960 1970 1980 1990 2000
LKGSLRNRNV KTDTGKESCD VVLLDENLLK MVRDNPLYIR SHSKSVLRTS
2010 2020 2030 2040 2050
IHSDAHFLSS HLIIDYSLLV GRDDTSNELV VGIIDYIRTF TWDKKLEMVV
2060 2070 2080 2090
KSTGILGGQG KMPTVVSPEL YRTRFCEAMD KYFLMVPDHW TGLDLNC
Length:2,097
Mass (Da):236,877
Last modified:July 27, 2011 - v3
Checksum:i8C529E86FBEED8E6
GO
Isoform 2 (identifier: Q9Z1T6-1) [UniParc]FASTAAdd to basket
Also known as: p235L

The sequence of this isoform differs from the canonical sequence as follows:
     107-107: L → LENTLPHPQEST
     490-545: Missing.

Show »
Length:2,052
Mass (Da):232,081
Checksum:iC7FA5897A3319DA0
GO
Isoform 3 (identifier: Q9Z1T6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     490-497: NSASPSKR → SKFGFLML
     498-2097: Missing.

Show »
Length:497
Mass (Da):55,999
Checksum:i5BB1E9BE48047A7A
GO

Sequence cautioni

The sequence AAD10191 differs from that shown. Reason: Frameshift at several positions.Curated
The sequence BAB30626 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAD32355 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti546E → K in AAD10191 (PubMed:9858586).Curated1
Sequence conflicti944K → Q in BAD32355 (PubMed:15368895).Curated1
Sequence conflicti994 – 995QP → HR in AAD10191 (PubMed:9858586).Curated2
Sequence conflicti1107R → T in BAB30626 (PubMed:16141072).Curated1
Sequence conflicti1141L → V in AAD10191 (PubMed:9858586).Curated1
Sequence conflicti1264S → Y in AAD10191 (PubMed:9858586).Curated1
Sequence conflicti1996V → E in AAD10191 (PubMed:9858586).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_034953107L → LENTLPHPQEST in isoform 2. 1 Publication1
Alternative sequenceiVSP_034954490 – 545Missing in isoform 2. 1 PublicationAdd BLAST56
Alternative sequenceiVSP_034955490 – 497NSASPSKR → SKFGFLML in isoform 3. 1 Publication8
Alternative sequenceiVSP_034956498 – 2097Missing in isoform 3. 1 PublicationAdd BLAST1600

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF102777 mRNA Translation: AAD10191.1 Frameshift.
AK173077 mRNA Translation: BAD32355.1 Different initiation.
AK017186 mRNA Translation: BAB30626.3 Different initiation.
AK139116 mRNA Translation: BAE23894.1
AK165350 mRNA Translation: BAE38145.1
AC164079 Genomic DNA No translation available.
CCDSiCCDS35601.1 [Q9Z1T6-1]
CCDS78600.1 [Q9Z1T6-2]
PIRiT18290
RefSeqiNP_001297553.1, NM_001310624.1 [Q9Z1T6-2]
NP_035216.2, NM_011086.2
XP_006495844.1, XM_006495781.3 [Q9Z1T6-2]
UniGeneiMm.38370

Genome annotation databases

EnsembliENSMUST00000097707; ENSMUSP00000095314; ENSMUSG00000025949 [Q9Z1T6-2]
GeneIDi18711
KEGGimmu:18711
UCSCiuc007bho.1 mouse [Q9Z1T6-3]
uc007bht.1 mouse [Q9Z1T6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiFYV1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1T6
Secondary accession number(s): E9QL40
, Q3TNE4, Q3UTT6, Q69ZU1, Q9CU94
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 169 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health