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Protein

Connector enhancer of kinase suppressor of ras 2

Gene

Cnksr2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as an adapter protein or regulator of Ras signaling pathways, in synaptic junctions.

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Connector enhancer of kinase suppressor of ras 2
Short name:
Connector enhancer of KSR 2
Alternative name(s):
CNK homolog protein 2
Short name:
CNK2
Membrane-associated guanylate kinase-interacting protein
Short name:
Maguin
Gene namesi
Name:Cnksr2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi708454. Cnksr2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: Ensembl
  • Golgi apparatus Source: Ensembl
  • neuronal cell body Source: RGD
  • neuron projection Source: RGD
  • postsynaptic density Source: Ensembl
  • postsynaptic membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000899721 – 1032Connector enhancer of kinase suppressor of ras 2Add BLAST1032

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineBy similarity1
Modified residuei338PhosphoserineBy similarity1
Modified residuei390PhosphoserineCombined sources1
Modified residuei683PhosphotyrosineBy similarity1
Modified residuei685PhosphoserineCombined sources1
Modified residuei687PhosphoserineCombined sources1
Modified residuei756PhosphoserineCombined sources1
Modified residuei767PhosphoserineCombined sources1
Modified residuei906PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on tyrosine.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9Z1T4.
PRIDEiQ9Z1T4.

PTM databases

iPTMnetiQ9Z1T4.
PhosphoSitePlusiQ9Z1T4.

Expressioni

Tissue specificityi

Expressed in neurons and localized in the cell body and neurites.1 Publication

Gene expression databases

BgeeiENSRNOG00000007014.
GenevisibleiQ9Z1T4. RN.

Interactioni

Subunit structurei

Interacts with RAF1, RAB2L and RAL GTPase proteins (By similarity). Interacts with DLG4 and AIP1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-8548356,EBI-8548356
Dlg4P310164EBI-8548356,EBI-375655
LRRC7Q96NW72EBI-8548356,EBI-524275From a different organism.
Lrrc7P705873EBI-8548356,EBI-7798464

Protein-protein interaction databases

BioGridi248765. 1 interactor.
IntActiQ9Z1T4. 3 interactors.
MINTiMINT-1784567.
STRINGi10116.ENSRNOP00000009802.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1T4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 76SAMPROSITE-ProRule annotationAdd BLAST66
Domaini84 – 178CRICPROSITE-ProRule annotationAdd BLAST95
Domaini215 – 297PDZPROSITE-ProRule annotationAdd BLAST83
Domaini332 – 515DUF1170Add BLAST184
Domaini570 – 669PHPROSITE-ProRule annotationAdd BLAST100

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili874 – 917Sequence analysisAdd BLAST44

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi354 – 357Poly-Pro4
Compositional biasi703 – 706Poly-Pro4
Compositional biasi875 – 886Poly-GluAdd BLAST12

Sequence similaritiesi

Belongs to the CNKSR family.Curated
Contains 1 CRIC domain.PROSITE-ProRule annotation
Contains 1 DUF1170 domain.Curated
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1738. Eukaryota.
ENOG4110T89. LUCA.
GeneTreeiENSGT00390000017199.
HOGENOMiHOG000231501.
HOVERGENiHBG051040.
InParanoidiQ9Z1T4.
KOiK17536.
OMAiSTKLEYK.
OrthoDBiEOG091G037J.
PhylomeDBiQ9Z1T4.
TreeFamiTF326495.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR010599. CNKSR2.
IPR017874. CRIC_domain.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF10534. CRIC_ras_sig. 1 hit.
PF06663. DUF1170. 1 hit.
PF00595. PDZ. 1 hit.
PF00169. PH. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00233. PH. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS51290. CRIC. 1 hit.
PS50106. PDZ. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z1T4-1) [UniParc]FASTAAdd to basket
Also known as: Maguin-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALIMEPVSK WSPSQVVDWM KGLDDCLQQY IKNFEREKIS GDQLLRITHQ
60 70 80 90 100
ELEDLGVSRI GHQELILEAV DLLCALNYGL ETENLKTLSH KLNASAKNLQ
110 120 130 140 150
NFITGRRRSG HYDGRTSRKL PNDFLTSVVD LIGAAKSLLA WLDRSPFAAV
160 170 180 190 200
TDYSVTRNNV IQLCLELTTI VQQDCTVYET ENKILHVCKT LSGVCDHIIS
210 220 230 240 250
LSSDPLVSQS AHLEVIQLAN IKPSEGLGMY IKSTYDGLHV ITGTTENSPA
260 270 280 290 300
DRCKKIHAGD EVIQVNHQTV VGWQLKNLVN ALREDPSGVI LTLKKRPQSM
310 320 330 340 350
LTSAPALLKN MRWKPLALQP LIPRSPTSSV ATPSSTISTP TKRDSSALQD
360 370 380 390 400
LYIPPPPAEP YIPRDEKGNL PCEDLRGHMV GKPVHKGSES PNSFLDQEYR
410 420 430 440 450
KRFNIVEEDT VLYCYEYEKG RSSSQGRRES TPTYGKLRPI SMPVEYNWVG
460 470 480 490 500
DYEDPNKMKR DSRRENSLLR YMSNEKIAQE EYMFQRNSKK DTGKKSKKKG
510 520 530 540 550
DKSTSPTHYS LLPSLQMDAL RQDIMGTPVP ETTLYHTFQQ SSLQHKSKKK
560 570 580 590 600
NKGAIAGKSK RRISCKDLGR GDCEGWLWKK KDAKSYFSQK WKKYWFVLKD
610 620 630 640 650
ASLYWYINEE DEKAEGFISL PEFKIDRASE CRKKYAFKAC HPKIKSFYFA
660 670 680 690 700
AEHLDDMNRW LNRINMLTAG YAERERIKQE QDYWSESDKE EADTPSTPKQ
710 720 730 740 750
DSPPPPYDTY PRPPSMSCAS PYVEAKHSRL SSTETSQSQS SHEEFRQEVT
760 770 780 790 800
GSSAVSPIRK TASQRRSWQD LIETPLTSSG LHYLQTLPLE DSVFSDSAAI
810 820 830 840 850
SPEHRRQSTL PTQKCHLQDH YGPYPLAESE RMQVLNGNGG KPRSFTLPRD
860 870 880 890 900
SGFNHCCLNA PVSACDPQDD IQPPEVEEEE EEEEEEAAGE NIGEKNENRE
910 920 930 940 950
EKLGDSLQDL YRALEEASLS PLGEHRISTK IEYKLSFIKR CNDPVMNEKL
960 970 980 990 1000
HRLRILKSTL KAREGEVAII DKVLDNPDLT SKEFQQWKQM YLDLFLDICQ
1010 1020 1030
NTTSNDPLSI SSEVDVITSS LTHTHSYIET HV
Length:1,032
Mass (Da):117,390
Last modified:May 1, 1999 - v1
Checksum:i02BD762461F8D7EC
GO
Isoform 2 (identifier: Q9Z1T4-2) [UniParc]FASTAAdd to basket
Also known as: Maguin-2

The sequence of this isoform differs from the canonical sequence as follows:
     896-896: N → S
     897-1032: Missing.

Show »
Length:896
Mass (Da):101,663
Checksum:iD8F73127405D1AA7
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010891896N → S in isoform 2. 1 Publication1
Alternative sequenceiVSP_010892897 – 1032Missing in isoform 2. 1 PublicationAdd BLAST136

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF102853 mRNA. Translation: AAD04567.1.
AF102854 mRNA. Translation: AAD04568.1.
PIRiT18293.
RefSeqiNP_001106837.1. NM_001113366.1. [Q9Z1T4-1]
NP_067718.1. NM_021686.3. [Q9Z1T4-2]
UniGeneiRn.42893.

Genome annotation databases

EnsembliENSRNOT00000009802; ENSRNOP00000009802; ENSRNOG00000007014. [Q9Z1T4-1]
ENSRNOT00000080512; ENSRNOP00000071915; ENSRNOG00000007014. [Q9Z1T4-2]
GeneIDi59322.
KEGGirno:59322.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF102853 mRNA. Translation: AAD04567.1.
AF102854 mRNA. Translation: AAD04568.1.
PIRiT18293.
RefSeqiNP_001106837.1. NM_001113366.1. [Q9Z1T4-1]
NP_067718.1. NM_021686.3. [Q9Z1T4-2]
UniGeneiRn.42893.

3D structure databases

ProteinModelPortaliQ9Z1T4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248765. 1 interactor.
IntActiQ9Z1T4. 3 interactors.
MINTiMINT-1784567.
STRINGi10116.ENSRNOP00000009802.

PTM databases

iPTMnetiQ9Z1T4.
PhosphoSitePlusiQ9Z1T4.

Proteomic databases

PaxDbiQ9Z1T4.
PRIDEiQ9Z1T4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009802; ENSRNOP00000009802; ENSRNOG00000007014. [Q9Z1T4-1]
ENSRNOT00000080512; ENSRNOP00000071915; ENSRNOG00000007014. [Q9Z1T4-2]
GeneIDi59322.
KEGGirno:59322.

Organism-specific databases

CTDi22866.
RGDi708454. Cnksr2.

Phylogenomic databases

eggNOGiKOG1738. Eukaryota.
ENOG4110T89. LUCA.
GeneTreeiENSGT00390000017199.
HOGENOMiHOG000231501.
HOVERGENiHBG051040.
InParanoidiQ9Z1T4.
KOiK17536.
OMAiSTKLEYK.
OrthoDBiEOG091G037J.
PhylomeDBiQ9Z1T4.
TreeFamiTF326495.

Miscellaneous databases

PROiQ9Z1T4.

Gene expression databases

BgeeiENSRNOG00000007014.
GenevisibleiQ9Z1T4. RN.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR010599. CNKSR2.
IPR017874. CRIC_domain.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF10534. CRIC_ras_sig. 1 hit.
PF06663. DUF1170. 1 hit.
PF00595. PDZ. 1 hit.
PF00169. PH. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00233. PH. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS51290. CRIC. 1 hit.
PS50106. PDZ. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCNKR2_RAT
AccessioniPrimary (citable) accession number: Q9Z1T4
Secondary accession number(s): Q9R093
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.