Thrombospondin-4 precursor - Mus musculus (Mouse)

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Q9Z1T2 (TSP4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 95. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Thrombospondin-4

Gene names

Name:	Thbs4
Synonyms:	Tsp4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	963 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Acts as an extracellular mitogen and stimulates early erythroid progenitors proliferation in presence of EPO. Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen By similarity.

Subunit structure

Homopentamer; disulfide-linked. Interacts with PTBP3 By similarity.

Sequence similarities

Belongs to the thrombospondin family.

Contains 4 EGF-like domains.

Contains 1 laminin G-like domain.

Contains 1 TSP C-terminal (TSPC) domain.

Contains 8 TSP type-3 repeats.

Ontologies

Keywords
Biological process	Cell adhesion
Domain	EGF-like domain Repeat Signal
Ligand	Calcium
Molecular function	Growth factor Mitogen
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endothelial cell-cell adhesion Inferred from electronic annotation. Source: Compara myoblast migration Inferred from electronic annotation. Source: Compara negative regulation of angiogenesis Inferred from electronic annotation. Source: Compara positive regulation of cell division Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of endothelial cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of neutrophil chemotaxis Inferred from electronic annotation. Source: Compara positive regulation of peptidyl-tyrosine phosphorylation Inferred from electronic annotation. Source: Compara
Cellular_component	basement membrane Inferred from electronic annotation. Source: Compara extracellular space Inferred from electronic annotation. Source: Compara
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro heparin binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Atf6	B2RU98	3	EBI-6171531,EBI-6171558

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

Potential

Chain

27 – 963

937

Thrombospondin-4

PRO_0000035853

Regions

Domain

29 – 194

166

Laminin G-like

Domain

288 – 327

EGF-like 1

Domain

328 – 365

EGF-like 2; calcium-binding Potential

Domain

381 – 418

EGF-like 3; calcium-binding Potential

Domain

422 – 464

EGF-like 4

Repeat

465 – 497

TSP type-3 1

Repeat

498 – 533

TSP type-3 2

Repeat

534 – 556

TSP type-3 3

Repeat

557 – 592

TSP type-3 4

Repeat

593 – 615

TSP type-3 5

Repeat

616 – 653

TSP type-3 6

Repeat

654 – 693

TSP type-3 7

Repeat

694 – 729

TSP type-3 8

Domain

733 – 947

215

TSP C-terminal

Motif

138 – 140

Cell attachment site Potential

Motif

564 – 566

Cell attachment site Potential

Amino acid modifications

Glycosylation

614

N-linked (GlcNAc...) Potential

Glycosylation

650

N-linked (GlcNAc...) Potential

Glycosylation

943

N-linked (GlcNAc...) Potential

Disulfide bond

260

Interchain By similarity

Disulfide bond

263

Interchain By similarity

Disulfide bond

292 ↔ 303

By similarity

Disulfide bond

297 ↔ 312

By similarity

Disulfide bond

315 ↔ 326

By similarity

Disulfide bond

332 ↔ 343

By similarity

Disulfide bond

337 ↔ 352

By similarity

Disulfide bond

355 ↔ 379

By similarity

Disulfide bond

385 ↔ 396

By similarity

Disulfide bond

390 ↔ 405

By similarity

Disulfide bond

408 ↔ 420

By similarity

Disulfide bond

426 ↔ 440

By similarity

Disulfide bond

434 ↔ 450

By similarity

Disulfide bond

452 ↔ 463

By similarity

Disulfide bond

479 ↔ 484

By similarity

Disulfide bond

489 ↔ 509

By similarity

Disulfide bond

525 ↔ 545

By similarity

Disulfide bond

548 ↔ 568

By similarity

Disulfide bond

584 ↔ 604

By similarity

Disulfide bond

607 ↔ 627

By similarity

Disulfide bond

645 ↔ 665

By similarity

Disulfide bond

685 ↔ 705

By similarity

Disulfide bond

721 ↔ 942

By similarity

Experimental info

Sequence conflict

S → P in AAD27642. Ref.1

Sequence conflict

290

R → H in AAD32714. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q9Z1T2 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: B8BA83B84F489FB1
Show »

FASTA

963

106,366

        10         20         30         40         50         60 
MPAPRAAAAA FLLLHLVLQP WQRTSAQATP QVFDLLPSSS QRLNPSALQP VLTDPTLHEV 

        70         80         90        100        110        120 
YLISTFKLQS KSSATIFGLY SSSDNSKYFE FTVMGRLNKA ILRYLKNDGK IHLVVFNNLQ 

       130        140        150        160        170        180 
LADGRRHRVL LRLSNLQRGD GSVELYLDCA QADSVRNLPR AFSGLTQNPE SIELRTFQRK 

       190        200        210        220        230        240 
PQDFLEELKL VVRGSLFQVA SLQDCFLQQS EPLAATSTGD FNRQFLGQMT QLNQLLGEVK 

       250        260        270        280        290        300 
DLLRQQVKET SFLRNTIAEC QACGPLSFQS PTPNTLVPIA PPAPPTRPTR HCDSSPCFRG 

       310        320        330        340        350        360 
VRCTDTRDGF QCGPCPDGYT GNGITCSDVD ECKYHPCYPG VRCVNLAPGF RCDACPVGFT 

       370        380        390        400        410        420 
GPMVQGVGIN FAKTNKQVCT DVDECQNGAC VLNSICINTL GSYRCGPCKP GYTGDQTRGC 

       430        440        450        460        470        480 
KTERSCRNPE QNPCSVHAQC IEERQGDVTC VCGVGWAGDG YVCGKDVDID SYPDEELPCS 

       490        500        510        520        530        540 
ARNCKKDNCK YVPNSGQEDA DRDGIGDACD EDADGDGILN EQDNCVLTHN IDQRNSDKDI 

       550        560        570        580        590        600 
FGDACDNCRM VLNNDQKDTD GDGRGDACDD DMDGDGIKNI LDNCPRVPNR DQQDRDGDDV 

       610        620        630        640        650        660 
GDACDSCPDV SNPNQSDVDN DLVGDSCDTN QDSDGDGHQD STDNCPTVIN SSQLDTDKDG 

       670        680        690        700        710        720 
IGDECDDDDD NDGIPDLVPP GPDNCRLVPN PAQEDSNNDG VGDICEADFD QDQVIDHIDV 

       730        740        750        760        770        780 
CPENAEITLT DFRAYQTVVL DPEGDAQIDP NWVVLNQGME IVQTMNSDPG LAVGYTAFNG 

       790        800        810        820        830        840 
VDFEGTFHVN TQTDDDYAGF IFGYQDSSSF YVVMWKQTEQ TYWQATPFRA VAEPGIQLKA 

       850        860        870        880        890        900 
VKSKTGPGEH LRNSLWHTGD TSDQVRLLWK DSRNVGWKDK VSYRWFLQHR PQVGYIRVRF 

       910        920        930        940        950        960 
YEGSELVADS GVTIDTTMRG GRLGVFCFSQ ENIIWSNLKY RCNDTIPEDF QEFQTQSFDR 


LDN

« Hide

References

[1]	"The thrombospondin-4 gene." Newton G., Weremowicz S., Morton C.C., Jenkins N.A., Gilbert D.J., Copeland N.G., Lawler J. Mamm. Genome 10:1010-1016(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF102887 mRNA. Translation: AAC73003.1. AF130461 Genomic DNA. Translation: AAD27642.1. AH007739 Genomic DNA. Translation: AAD32714.1.
IPI	IPI00130445.
RefSeq	NP_035712.1. NM_011582.3.
UniGene	Mm.20865.
3D structure databases
ProteinModelPortal	Q9Z1T2.
SMR	Q9Z1T2. Positions 220-264, 292-954.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Z1T2. 1 interaction.
PTM databases
PhosphoSite	Q9Z1T2.
Proteomic databases
PaxDb	Q9Z1T2.
PRIDE	Q9Z1T2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022213; ENSMUSP00000022213; ENSMUSG00000021702.
GeneID	21828.
KEGG	mmu:21828.
Organism-specific databases
CTD	7060.
MGI	MGI:1101779. Thbs4.
Phylogenomic databases
eggNOG	NOG12793.
GeneTree	ENSGT00550000074507.
HOGENOM	HOG000007542.
HOVERGEN	HBG000636.
InParanoid	Q9Z1T2.
KO	K04659.
OMA	DSNPCFR.
OrthoDB	EOG4X0MRM.
Gene expression databases
ArrayExpress	Q9Z1T2.
Bgee	Q9Z1T2.
CleanEx	MM_THBS4.
Genevestigator	Q9Z1T2.
GermOnline	ENSMUSG00000021702. Mus musculus.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000742. EG-like_dom. IPR001881. EGF-like_Ca-bd. IPR013032. EGF-like_CS. IPR018097. EGF_Ca-bd_CS. IPR001791. Laminin_G. IPR024665. Thbs/COMP_coiled-coil. IPR003367. Thrombospondin_3-like_rpt. IPR017897. Thrombospondin_3_rpt. IPR008859. Thrombospondin_C. [Graphical view]
Pfam	PF11598. COMP. 1 hit. PF00008. EGF. 1 hit. PF07645. EGF_CA. 2 hits. PF02412. TSP_3. 5 hits. PF05735. TSP_C. 1 hit. [Graphical view]
SMART	SM00181. EGF. 2 hits. SM00179. EGF_CA. 2 hits. SM00210. TSPN. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 2 hits.
PROSITE	PS00022. EGF_1. False negative. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 4 hits. PS01187. EGF_CA. 2 hits. PS50025. LAM_G_DOMAIN. False negative. PS51234. TSP3. 8 hits. PS51236. TSP_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	301264.
SOURCE	Search...

Entry information

Entry name

TSP4_MOUSE

Accession

Primary (citable) accession number: Q9Z1T2
Secondary accession number(s): Q9QYS3, Q9WUE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents