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Q9Z1T2

- TSP4_MOUSE

UniProt

Q9Z1T2 - TSP4_MOUSE

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Protein

Thrombospondin-4

Gene

Thbs4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner.6 Publications

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. heparin binding Source: Ensembl

GO - Biological processi

  1. behavioral response to pain Source: UniProtKB
  2. endothelial cell-cell adhesion Source: Ensembl
  3. myoblast migration Source: Ensembl
  4. negative regulation of angiogenesis Source: Ensembl
  5. positive regulation of cell division Source: UniProtKB-KW
  6. positive regulation of endothelial cell proliferation Source: Ensembl
  7. positive regulation of neutrophil chemotaxis Source: Ensembl
  8. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  9. regulation of tissue remodeling Source: UniProtKB
  10. response to endoplasmic reticulum stress Source: UniProtKB
  11. response to unfolded protein Source: UniProtKB-KW
  12. tissue remodeling Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Mitogen

Keywords - Biological processi

Cell adhesion, Tissue remodeling, Unfolded protein response

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_263353. Signaling by PDGF.

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-4
Gene namesi
Name:Thbs4
Synonyms:Tsp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:1101779. Thbs4.

Subcellular locationi

GO - Cellular componenti

  1. basement membrane Source: Ensembl
  2. endoplasmic reticulum Source: UniProtKB
  3. extracellular matrix Source: UniProtKB
  4. extracellular space Source: UniProtKB
  5. sarcoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Extracellular matrix, Sarcoplasmic reticulum, Secreted

Pathology & Biotechi

Disruption phenotypei

On exposure to acute pressure overload, mice exhibit a marked increase in: heart weight and fibrosis, cardiomyocyte size and number of apoptotic cells in the myocardium, deposition of extracellular matrix (ECM) and levels of interstitial collagens. The increased ECM deposition is accompanied by changes in functional parameters of the heart and decreased vessel density. Mice also show defective induction of the ER stress response in the heart. Do not exhibit peripheral nerve injury-induced behavioral hypersensitivities such as thermal/mechanical hyperalgesia and tactile allodynia but show severe defects in cortical-injury-induced subventricular zone astrogenesis.4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 963937Thrombospondin-4PRO_0000035853Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi260 – 260InterchainPROSITE-ProRule annotation
Disulfide bondi263 – 263InterchainPROSITE-ProRule annotation
Disulfide bondi292 ↔ 303PROSITE-ProRule annotation
Disulfide bondi297 ↔ 312PROSITE-ProRule annotation
Disulfide bondi315 ↔ 326PROSITE-ProRule annotation
Disulfide bondi332 ↔ 343PROSITE-ProRule annotation
Disulfide bondi337 ↔ 352PROSITE-ProRule annotation
Disulfide bondi355 ↔ 379PROSITE-ProRule annotation
Disulfide bondi385 ↔ 396PROSITE-ProRule annotation
Disulfide bondi390 ↔ 405PROSITE-ProRule annotation
Disulfide bondi408 ↔ 420PROSITE-ProRule annotation
Disulfide bondi426 ↔ 440PROSITE-ProRule annotation
Disulfide bondi434 ↔ 450PROSITE-ProRule annotation
Disulfide bondi452 ↔ 463PROSITE-ProRule annotation
Disulfide bondi479 ↔ 484PROSITE-ProRule annotation
Disulfide bondi489 ↔ 509PROSITE-ProRule annotation
Disulfide bondi525 ↔ 545PROSITE-ProRule annotation
Disulfide bondi548 ↔ 568PROSITE-ProRule annotation
Disulfide bondi584 ↔ 604PROSITE-ProRule annotation
Disulfide bondi607 ↔ 627PROSITE-ProRule annotation
Glycosylationi614 – 6141N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi645 ↔ 665PROSITE-ProRule annotation
Glycosylationi650 – 6501N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi685 ↔ 705PROSITE-ProRule annotation
Disulfide bondi721 ↔ 942PROSITE-ProRule annotation
Glycosylationi943 – 9431N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9Z1T2.
PaxDbiQ9Z1T2.
PRIDEiQ9Z1T2.

PTM databases

PhosphoSiteiQ9Z1T2.

Expressioni

Tissue specificityi

Heart. Up-regulated in the heart in response to ischemic injury and pathology (at protein level). Astrocytes; expressed at high levels in subventricular zone (SVZ)-derived astrocytes and at low levels in cortical astrocytes. In response to peripheral nerve injury, significantly up-regulated in the dorsal spinal cord (at protein level).3 Publications

Gene expression databases

BgeeiQ9Z1T2.
CleanExiMM_THBS4.
ExpressionAtlasiQ9Z1T2. baseline and differential.
GenevestigatoriQ9Z1T2.

Interactioni

Subunit structurei

Homopentamer; disulfide-linked. Interacts with PTBP3 (By similarity). Interacts (via EGF-like 3; calcium-binding domain) with ATF6 and facilitates its processing, activation and nuclear translocation. Interacts with NOTCH1.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Atf6F6VAN03EBI-6171531,EBI-6171558

Protein-protein interaction databases

IntActiQ9Z1T2. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1T2.
SMRiQ9Z1T2. Positions 220-264, 292-954.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 194166Laminin G-likeAdd
BLAST
Domaini288 – 32740EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini328 – 36538EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini381 – 41838EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini422 – 46443EGF-like 4PROSITE-ProRule annotationAdd
BLAST
Repeati465 – 49733TSP type-3 1Add
BLAST
Repeati498 – 53336TSP type-3 2Add
BLAST
Repeati534 – 55623TSP type-3 3Add
BLAST
Repeati557 – 59236TSP type-3 4Add
BLAST
Repeati593 – 61523TSP type-3 5Add
BLAST
Repeati616 – 65338TSP type-3 6Add
BLAST
Repeati654 – 69340TSP type-3 7Add
BLAST
Repeati694 – 72936TSP type-3 8Add
BLAST
Domaini733 – 947215TSP C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi138 – 1403Cell attachment siteSequence Analysis
Motifi564 – 5663Cell attachment siteSequence Analysis

Sequence similaritiesi

Belongs to the thrombospondin family.Curated
Contains 4 EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin G-like domain.Curated
Contains 1 TSP C-terminal (TSPC) domain.PROSITE-ProRule annotation
Contains 8 TSP type-3 repeats.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00550000074507.
HOGENOMiHOG000007542.
HOVERGENiHBG000636.
InParanoidiQ9Z1T2.
KOiK04659.
OMAiAECQACG.
OrthoDBiEOG76QFGD.
PhylomeDBiQ9Z1T2.
TreeFamiTF324917.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PfamiPF11598. COMP. 1 hit.
PF00008. EGF. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 5 hits.
PF05735. TSP_C. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z1T2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPAPRAAAAA FLLLHLVLQP WQRTSAQATP QVFDLLPSSS QRLNPSALQP
60 70 80 90 100
VLTDPTLHEV YLISTFKLQS KSSATIFGLY SSSDNSKYFE FTVMGRLNKA
110 120 130 140 150
ILRYLKNDGK IHLVVFNNLQ LADGRRHRVL LRLSNLQRGD GSVELYLDCA
160 170 180 190 200
QADSVRNLPR AFSGLTQNPE SIELRTFQRK PQDFLEELKL VVRGSLFQVA
210 220 230 240 250
SLQDCFLQQS EPLAATSTGD FNRQFLGQMT QLNQLLGEVK DLLRQQVKET
260 270 280 290 300
SFLRNTIAEC QACGPLSFQS PTPNTLVPIA PPAPPTRPTR HCDSSPCFRG
310 320 330 340 350
VRCTDTRDGF QCGPCPDGYT GNGITCSDVD ECKYHPCYPG VRCVNLAPGF
360 370 380 390 400
RCDACPVGFT GPMVQGVGIN FAKTNKQVCT DVDECQNGAC VLNSICINTL
410 420 430 440 450
GSYRCGPCKP GYTGDQTRGC KTERSCRNPE QNPCSVHAQC IEERQGDVTC
460 470 480 490 500
VCGVGWAGDG YVCGKDVDID SYPDEELPCS ARNCKKDNCK YVPNSGQEDA
510 520 530 540 550
DRDGIGDACD EDADGDGILN EQDNCVLTHN IDQRNSDKDI FGDACDNCRM
560 570 580 590 600
VLNNDQKDTD GDGRGDACDD DMDGDGIKNI LDNCPRVPNR DQQDRDGDDV
610 620 630 640 650
GDACDSCPDV SNPNQSDVDN DLVGDSCDTN QDSDGDGHQD STDNCPTVIN
660 670 680 690 700
SSQLDTDKDG IGDECDDDDD NDGIPDLVPP GPDNCRLVPN PAQEDSNNDG
710 720 730 740 750
VGDICEADFD QDQVIDHIDV CPENAEITLT DFRAYQTVVL DPEGDAQIDP
760 770 780 790 800
NWVVLNQGME IVQTMNSDPG LAVGYTAFNG VDFEGTFHVN TQTDDDYAGF
810 820 830 840 850
IFGYQDSSSF YVVMWKQTEQ TYWQATPFRA VAEPGIQLKA VKSKTGPGEH
860 870 880 890 900
LRNSLWHTGD TSDQVRLLWK DSRNVGWKDK VSYRWFLQHR PQVGYIRVRF
910 920 930 940 950
YEGSELVADS GVTIDTTMRG GRLGVFCFSQ ENIIWSNLKY RCNDTIPEDF
960
QEFQTQSFDR LDN
Length:963
Mass (Da):106,366
Last modified:May 1, 1999 - v1
Checksum:iB8BA83B84F489FB1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401S → P in AAD27642. (PubMed:10501972)Curated
Sequence conflicti290 – 2901R → H in AAD32714. (PubMed:10501972)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF102887 mRNA. Translation: AAC73003.1.
AF130461 Genomic DNA. Translation: AAD27642.1.
AH007739 Genomic DNA. Translation: AAD32714.1.
CCDSiCCDS26684.1.
RefSeqiNP_035712.1. NM_011582.3.
UniGeneiMm.20865.

Genome annotation databases

EnsembliENSMUST00000022213; ENSMUSP00000022213; ENSMUSG00000021702.
GeneIDi21828.
KEGGimmu:21828.
UCSCiuc007rku.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF102887 mRNA. Translation: AAC73003.1 .
AF130461 Genomic DNA. Translation: AAD27642.1 .
AH007739 Genomic DNA. Translation: AAD32714.1 .
CCDSi CCDS26684.1.
RefSeqi NP_035712.1. NM_011582.3.
UniGenei Mm.20865.

3D structure databases

ProteinModelPortali Q9Z1T2.
SMRi Q9Z1T2. Positions 220-264, 292-954.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9Z1T2. 1 interaction.

PTM databases

PhosphoSitei Q9Z1T2.

Proteomic databases

MaxQBi Q9Z1T2.
PaxDbi Q9Z1T2.
PRIDEi Q9Z1T2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022213 ; ENSMUSP00000022213 ; ENSMUSG00000021702 .
GeneIDi 21828.
KEGGi mmu:21828.
UCSCi uc007rku.2. mouse.

Organism-specific databases

CTDi 7060.
MGIi MGI:1101779. Thbs4.

Phylogenomic databases

eggNOGi NOG12793.
GeneTreei ENSGT00550000074507.
HOGENOMi HOG000007542.
HOVERGENi HBG000636.
InParanoidi Q9Z1T2.
KOi K04659.
OMAi AECQACG.
OrthoDBi EOG76QFGD.
PhylomeDBi Q9Z1T2.
TreeFami TF324917.

Enzyme and pathway databases

Reactomei REACT_263353. Signaling by PDGF.

Miscellaneous databases

NextBioi 301264.
PROi Q9Z1T2.
SOURCEi Search...

Gene expression databases

Bgeei Q9Z1T2.
CleanExi MM_THBS4.
ExpressionAtlasi Q9Z1T2. baseline and differential.
Genevestigatori Q9Z1T2.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProi IPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view ]
Pfami PF11598. COMP. 1 hit.
PF00008. EGF. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 5 hits.
PF05735. TSP_C. 1 hit.
[Graphical view ]
SMARTi SM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00210. TSPN. 1 hit.
[Graphical view ]
SUPFAMi SSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEi PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. Cited for: FUNCTION.
  3. "Thrombospondin-4 is required for stretch-mediated contractility augmentation in cardiac muscle."
    Cingolani O.H., Kirk J.A., Seo K., Koitabashi N., Lee D.I., Ramirez-Correa G., Bedja D., Barth A.S., Moens A.L., Kass D.A.
    Circ. Res. 109:1410-1414(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  4. Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATF6.
  5. "Thrombospondin-4 regulates fibrosis and remodeling of the myocardium in response to pressure overload."
    Frolova E.G., Sopko N., Blech L., Popovic Z.B., Li J., Vasanji A., Drumm C., Krukovets I., Jain M.K., Penn M.S., Plow E.F., Stenina O.I.
    FASEB J. 26:2363-2373(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  6. Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  7. "Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4."
    Benner E.J., Luciano D., Jo R., Abdi K., Paez-Gonzalez P., Sheng H., Warner D.S., Liu C., Eroglu C., Kuo C.T.
    Nature 497:369-373(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION WITH NOTCH1.

Entry informationi

Entry nameiTSP4_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1T2
Secondary accession number(s): Q9QYS3, Q9WUE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 1, 1999
Last modified: November 26, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3