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Q9Z1T1 (AP3B1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-3 complex subunit beta-1
Alternative name(s):
Adapter-related protein complex 3 subunit beta-1
Adaptor protein complex AP-3 subunit beta-1
Beta-3A-adaptin
Clathrin assembly protein complex 3 beta-1 large chain
Gene names
Name:Ap3b1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1105 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. Ref.7

Subunit structure

Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2). AP-3 associates with the BLOC-1 complex.

Subcellular location

Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Golgi apparatus By similarity. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex By similarity.

Tissue specificity

Ubiquitously expressed.

Involvement in disease

Defects in Ap3b1 are the cause of the autosomal recessive phenotype 'pearl' (pe). Pearl mice exhibit hypopigmentation, lysosomal secretion abnormalities, and platelet-dense granules with reduced levels of adenine nucleotides and serotonin. The changes in platelets lead to prolonged bleeding. Additionally, pearl mice exhibit reduced sensitivity in the dark-adapted state. Ref.1

Sequence similarities

Belongs to the adaptor complexes large subunit family.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCytoplasmic vesicle
Golgi apparatus
Membrane
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanterograde axon cargo transport

Inferred from mutant phenotype Ref.7. Source: UniProtKB

anterograde synaptic vesicle transport

Inferred from mutant phenotype Ref.7. Source: UniProtKB

antigen processing and presentation

Inferred from mutant phenotype PubMed 14557411. Source: MGI

antigen processing and presentation, exogenous lipid antigen via MHC class Ib

Inferred from mutant phenotype PubMed 14557411. Source: MGI

blood coagulation

Inferred from mutant phenotype PubMed 6696991. Source: MGI

intracellular protein transport

Traceable author statement PubMed 9714600. Source: MGI

melanosome organization

Inferred from electronic annotation. Source: Ensembl

positive regulation of NK T cell differentiation

Inferred from mutant phenotype PubMed 14557411. Source: MGI

protein targeting to lysosome

Inferred from mutant phenotype PubMed 14557411. Source: MGI

vesicle-mediated transport

Traceable author statement PubMed 9714600. Source: MGI

zinc ion transport

Traceable author statement PubMed 17349999. Source: BHF-UCL

   Cellular_componentAP-3 adaptor complex

Inferred from electronic annotation. Source: InterPro

Golgi apparatus

Inferred from electronic annotation. Source: UniProtKB-SubCell

clathrin-coated vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

trans-Golgi network

Traceable author statement PubMed 9714600. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11051105AP-3 complex subunit beta-1
PRO_0000193747

Regions

Compositional bias678 – 802125Glu/Ser-rich

Amino acid modifications

Modified residue2761Phosphoserine Ref.5 Ref.6
Modified residue6101Phosphoserine By similarity
Modified residue7611Phosphoserine By similarity
Modified residue7631Phosphoserine By similarity

Experimental info

Sequence conflict275 – 2773ESE → DS in AAH15068. Ref.4
Sequence conflict439 – 4446VTDTCL → FTETCF in AAC78338. Ref.1
Sequence conflict439 – 4446VTDTCL → FTETCF in AAG23622. Ref.2
Sequence conflict4871L → F in AAC78338. Ref.1
Sequence conflict4871L → F in AAG23622. Ref.2
Sequence conflict6911D → DEE in AAH15068. Ref.4
Sequence conflict7141G → E in AAH15068. Ref.4
Sequence conflict7241T → TSN in AAH15068. Ref.4
Sequence conflict7371S → N in AAH15068. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9Z1T1 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 59EB4A722B40E233

FASTA1,105122,740
        10         20         30         40         50         60 
MSSNSFAYNE QSGGGEAAEL GQEATSTISP SGAFGLFSSD WKKNEDLKQM LESNKDSAKL 

        70         80         90        100        110        120 
DAMKRIVGMI AKGKNASELF PAVVKNVASK NIEIKKLVYV YLVRYAEEQQ DLALLSISTF 

       130        140        150        160        170        180 
QRALKDPNQL IRASALRVLS SIRVPIIVPV MMLAIKEASA DLSPYVRKNA AHAIQKLYSL 

       190        200        210        220        230        240 
DPEQKEMLIE VIEKLLKDKS TLVAGSVVMA FEEVCPDRID LIHRNYRKLC NLLVDVEEWG 

       250        260        270        280        290        300 
QVVIIHMLTR YARTQFVSPW REDGGLEDNE KNFYESEEEE EEKEKSSRKK SYAMDPDHRL 

       310        320        330        340        350        360 
LIRNTKPLLQ SRNAAVVMAV AQLYWHISPK SEAGVISKSL VRLLRSNREV QYIVLQNIAT 

       370        380        390        400        410        420 
MSIERKGMFE PYLKSFYVRS TDPTMIKTLK LEILTNLANE ANISTLLREF QTYVRSQDKQ 

       430        440        450        460        470        480 
FAAATIQTIG RCATSISEVT DTCLNGLVCL LSNRDEIVVA ESVVVIKKLL QMQPAQHGEI 

       490        500        510        520        530        540 
IRHMAKLLDS ITVPVARASI LWLIGENCER VPKIAPDVLR KMAKSFTSED DLVKLQILNL 

       550        560        570        580        590        600 
AAKLYLTNSK QTKLLTQYIL NLGKYDQNYD IRDRTRFIRQ LIVPNEKSGA LSKYAKKIFL 

       610        620        630        640        650        660 
APKPAPLLES PFKDRDRFQL GTLSHTLNIK ASGYLELSNW PEVAPDPSVR NVEVIESAKE 

       670        680        690        700        710        720 
WTPLGKTKKE KPMKKFYSES EEEEDEDEDE DEEEEEKEDE DENPSDSSSD SESGSGSESG 

       730        740        750        760        770        780 
DTGTEDSSED SSSGQDSETG SQAEAERQKV AKRNSKTKRK SDSENREKKN ENSKASESSS 

       790        800        810        820        830        840 
EESSSMEDSS SESESESGSD SEPAPRNVAP AKERKPQQER HPPSKDVFLL DLDDFNPVST 

       850        860        870        880        890        900 
PVALPTPALS PSLIADLEGL NLSTSSSVIN VSTPVFVPTK THELLHRMHG KGLAAHYCFP 

       910        920        930        940        950        960 
RQPCIFSDKM VSVQITLTNT SDRKIENIHI GGKGLPVGMQ MHAFHPIDSL EPKGSVTVSV 

       970        980        990       1000       1010       1020 
GIDFCDSTQT ASFQLCTKDD CFNVTLQPPV GELLSPVAMS EKDFKKEQGT LTGMNETSAT 

      1030       1040       1050       1060       1070       1080 
LIAAPQNFTP SMILQKVVNV ANLGAVPSSQ DNVHRFAART VHSGSLMLVT VELKEGSTAQ 

      1090       1100 
LIINTEKTVI GSVLLRELKP VLSQG 

« Hide

References

« Hide 'large scale' references
[1]"The beta-3A subunit gene (Ap3b1) of the AP-3 adaptor complex is altered in the mouse hypopigmentation mutant pearl, a model for Hermansky-Pudlak syndrome and night blindness."
Feng L., Seymour A.B., Jiang S.Y., To A., Peden A.A., Novak E.K., Zhen L., Rusiniak M.E., Eicher E.M., Robinson M.S., Gorin M.B., Swank R.T.
Hum. Mol. Genet. 8:323-330(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISEASE.
Strain: C3H/HeJ.
[2]"Genomic structure of the mouse ap3b1 gene in normal and pearl mice."
Feng L., Rigatti B.W., Novak E.K., Gorin M.B., Swank R.T.
Genomics 69:370-379(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse."
Larimore J., Tornieri K., Ryder P.V., Gokhale A., Zlatic S.A., Craige B., Lee J.D., Talbot K., Pare J.F., Smith Y., Faundez V.
Mol. Biol. Cell 22:4854-4867(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ASSOCIATION WITH THE BLOC-1 COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF103809 mRNA. Translation: AAC78338.1.
AF255589 expand/collapse EMBL AC list , AF255566, AF255567, AF255568, AF255569, AF255570, AF255571, AF255572, AF255573, AF255574, AF255575, AF255576, AF255577, AF255578, AF255579, AF255580, AF255581, AF255582, AF255583, AF255584, AF255585, AF255586, AF255587, AF255588 Genomic DNA. Translation: AAG23622.1.
AC122373 Genomic DNA. No translation available.
AC123043 Genomic DNA. No translation available.
BC015068 mRNA. Translation: AAH15068.1.
PIRT18295.
RefSeqNP_033810.2. NM_009680.3.
UniGeneMm.21185.

3D structure databases

ProteinModelPortalQ9Z1T1.
SMRQ9Z1T1. Positions 42-604.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198132. 3 interactions.
DIPDIP-49018N.
IntActQ9Z1T1. 5 interactions.
MINTMINT-1549674.

PTM databases

PhosphoSiteQ9Z1T1.

Proteomic databases

PaxDbQ9Z1T1.
PRIDEQ9Z1T1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022196; ENSMUSP00000022196; ENSMUSG00000021686.
GeneID11774.
KEGGmmu:11774.
UCSCuc007rlv.2. mouse.

Organism-specific databases

CTD8546.
MGIMGI:1333879. Ap3b1.

Phylogenomic databases

eggNOGCOG5096.
GeneTreeENSGT00530000063546.
HOGENOMHOG000033978.
HOVERGENHBG050519.
InParanoidQ9Z1T1.
KOK12397.
OMASGQDNIH.
OrthoDBEOG74R1QG.
TreeFamTF314605.

Gene expression databases

ArrayExpressQ9Z1T1.
BgeeQ9Z1T1.
CleanExMM_AP3B1.
GenevestigatorQ9Z1T1.

Family and domain databases

Gene3D1.25.10.10. 2 hits.
InterProIPR026740. AP3_beta.
IPR026739. AP_beta.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view]
PANTHERPTHR11134. PTHR11134. 1 hit.
PTHR11134:SF1. PTHR11134:SF1. 1 hit.
PfamPF01602. Adaptin_N. 1 hit.
[Graphical view]
PIRSFPIRSF037096. AP3_complex_beta. 1 hit.
SUPFAMSSF48371. SSF48371. 3 hits.
ProtoNetSearch...

Other

NextBio279563.
PROQ9Z1T1.
SOURCESearch...

Entry information

Entry nameAP3B1_MOUSE
AccessionPrimary (citable) accession number: Q9Z1T1
Secondary accession number(s): E9QQ08, Q91YR4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot