Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9Z1T1

- AP3B1_MOUSE

UniProt

Q9Z1T1 - AP3B1_MOUSE

Protein

AP-3 complex subunit beta-1

Gene

Ap3b1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.1 Publication

    GO - Biological processi

    1. anterograde axon cargo transport Source: UniProtKB
    2. anterograde synaptic vesicle transport Source: UniProtKB
    3. antigen processing and presentation Source: MGI
    4. antigen processing and presentation, exogenous lipid antigen via MHC class Ib Source: MGI
    5. blood coagulation Source: MGI
    6. intracellular protein transport Source: MGI
    7. melanosome organization Source: Ensembl
    8. positive regulation of NK T cell differentiation Source: MGI
    9. protein targeting to lysosome Source: MGI
    10. vesicle-mediated transport Source: MGI
    11. zinc ion transport Source: BHF-UCL

    Keywords - Biological processi

    Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AP-3 complex subunit beta-1
    Alternative name(s):
    Adaptor protein complex AP-3 subunit beta-1
    Adaptor-related protein complex 3 subunit beta-1
    Beta-3A-adaptin
    Clathrin assembly protein complex 3 beta-1 large chain
    Gene namesi
    Name:Ap3b1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:1333879. Ap3b1.

    Subcellular locationi

    Cytoplasmic vesicleclathrin-coated vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Golgi apparatus By similarity
    Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.By similarity

    GO - Cellular componenti

    1. AP-3 adaptor complex Source: InterPro
    2. clathrin-coated vesicle membrane Source: UniProtKB-SubCell
    3. lysosomal membrane Source: Ensembl
    4. trans-Golgi network Source: MGI

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Golgi apparatus, Membrane

    Pathology & Biotechi

    Involvement in diseasei

    Defects in Ap3b1 are the cause of the autosomal recessive phenotype 'pearl' (pe). Pearl mice exhibit hypopigmentation, lysosomal secretion abnormalities, and platelet-dense granules with reduced levels of adenine nucleotides and serotonin. The changes in platelets lead to prolonged bleeding. Additionally, pearl mice exhibit reduced sensitivity in the dark-adapted state (PubMed:9931340).1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11051105AP-3 complex subunit beta-1PRO_0000193747Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei276 – 2761Phosphoserine2 Publications
    Modified residuei610 – 6101PhosphoserineBy similarity
    Modified residuei761 – 7611PhosphoserineBy similarity
    Modified residuei763 – 7631PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9Z1T1.
    PaxDbiQ9Z1T1.
    PRIDEiQ9Z1T1.

    PTM databases

    PhosphoSiteiQ9Z1T1.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.

    Gene expression databases

    ArrayExpressiQ9Z1T1.
    BgeeiQ9Z1T1.
    CleanExiMM_AP3B1.
    GenevestigatoriQ9Z1T1.

    Interactioni

    Subunit structurei

    Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2). AP-3 associates with the BLOC-1 complex.

    Protein-protein interaction databases

    BioGridi198132. 4 interactions.
    DIPiDIP-49018N.
    IntActiQ9Z1T1. 6 interactions.
    MINTiMINT-1549674.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z1T1.
    SMRiQ9Z1T1. Positions 42-604.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi678 – 802125Glu/Ser-richAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG5096.
    GeneTreeiENSGT00530000063546.
    HOGENOMiHOG000033978.
    HOVERGENiHBG050519.
    InParanoidiQ9Z1T1.
    KOiK12397.
    OMAiFVPMKTH.
    OrthoDBiEOG74R1QG.
    TreeFamiTF314605.

    Family and domain databases

    Gene3Di1.25.10.10. 2 hits.
    InterProiIPR026740. AP3_beta.
    IPR029394. AP3B1_Ser.
    IPR029390. AP3B_C.
    IPR026739. AP_beta.
    IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR002553. Clathrin/coatomer_adapt-like_N.
    [Graphical view]
    PANTHERiPTHR11134. PTHR11134. 1 hit.
    PfamiPF01602. Adaptin_N. 1 hit.
    PF14796. AP3B1_C. 1 hit.
    PF14797. SEEEED. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037096. AP3_complex_beta. 1 hit.
    SUPFAMiSSF48371. SSF48371. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    Q9Z1T1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSNSFAYNE QSGGGEAAEL GQEATSTISP SGAFGLFSSD WKKNEDLKQM     50
    LESNKDSAKL DAMKRIVGMI AKGKNASELF PAVVKNVASK NIEIKKLVYV 100
    YLVRYAEEQQ DLALLSISTF QRALKDPNQL IRASALRVLS SIRVPIIVPV 150
    MMLAIKEASA DLSPYVRKNA AHAIQKLYSL DPEQKEMLIE VIEKLLKDKS 200
    TLVAGSVVMA FEEVCPDRID LIHRNYRKLC NLLVDVEEWG QVVIIHMLTR 250
    YARTQFVSPW REDGGLEDNE KNFYESEEEE EEKEKSSRKK SYAMDPDHRL 300
    LIRNTKPLLQ SRNAAVVMAV AQLYWHISPK SEAGVISKSL VRLLRSNREV 350
    QYIVLQNIAT MSIERKGMFE PYLKSFYVRS TDPTMIKTLK LEILTNLANE 400
    ANISTLLREF QTYVRSQDKQ FAAATIQTIG RCATSISEVT DTCLNGLVCL 450
    LSNRDEIVVA ESVVVIKKLL QMQPAQHGEI IRHMAKLLDS ITVPVARASI 500
    LWLIGENCER VPKIAPDVLR KMAKSFTSED DLVKLQILNL AAKLYLTNSK 550
    QTKLLTQYIL NLGKYDQNYD IRDRTRFIRQ LIVPNEKSGA LSKYAKKIFL 600
    APKPAPLLES PFKDRDRFQL GTLSHTLNIK ASGYLELSNW PEVAPDPSVR 650
    NVEVIESAKE WTPLGKTKKE KPMKKFYSES EEEEDEDEDE DEEEEEKEDE 700
    DENPSDSSSD SESGSGSESG DTGTEDSSED SSSGQDSETG SQAEAERQKV 750
    AKRNSKTKRK SDSENREKKN ENSKASESSS EESSSMEDSS SESESESGSD 800
    SEPAPRNVAP AKERKPQQER HPPSKDVFLL DLDDFNPVST PVALPTPALS 850
    PSLIADLEGL NLSTSSSVIN VSTPVFVPTK THELLHRMHG KGLAAHYCFP 900
    RQPCIFSDKM VSVQITLTNT SDRKIENIHI GGKGLPVGMQ MHAFHPIDSL 950
    EPKGSVTVSV GIDFCDSTQT ASFQLCTKDD CFNVTLQPPV GELLSPVAMS 1000
    EKDFKKEQGT LTGMNETSAT LIAAPQNFTP SMILQKVVNV ANLGAVPSSQ 1050
    DNVHRFAART VHSGSLMLVT VELKEGSTAQ LIINTEKTVI GSVLLRELKP 1100
    VLSQG 1105
    Length:1,105
    Mass (Da):122,740
    Last modified:July 27, 2011 - v2
    Checksum:i59EB4A722B40E233
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti275 – 2773ESE → DS in AAH15068. (PubMed:15489334)Curated
    Sequence conflicti439 – 4446VTDTCL → FTETCF in AAC78338. (PubMed:9931340)Curated
    Sequence conflicti439 – 4446VTDTCL → FTETCF in AAG23622. (PubMed:11056055)Curated
    Sequence conflicti487 – 4871L → F in AAC78338. (PubMed:9931340)Curated
    Sequence conflicti487 – 4871L → F in AAG23622. (PubMed:11056055)Curated
    Sequence conflicti691 – 6911D → DEE in AAH15068. (PubMed:15489334)Curated
    Sequence conflicti714 – 7141G → E in AAH15068. (PubMed:15489334)Curated
    Sequence conflicti724 – 7241T → TSN in AAH15068. (PubMed:15489334)Curated
    Sequence conflicti737 – 7371S → N in AAH15068. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF103809 mRNA. Translation: AAC78338.1.
    AF255589
    , AF255566, AF255567, AF255568, AF255569, AF255570, AF255571, AF255572, AF255573, AF255574, AF255575, AF255576, AF255577, AF255578, AF255579, AF255580, AF255581, AF255582, AF255583, AF255584, AF255585, AF255586, AF255587, AF255588 Genomic DNA. Translation: AAG23622.1.
    AC122373 Genomic DNA. No translation available.
    AC123043 Genomic DNA. No translation available.
    BC015068 mRNA. Translation: AAH15068.1.
    CCDSiCCDS26693.1.
    PIRiT18295.
    RefSeqiNP_033810.2. NM_009680.3.
    UniGeneiMm.21185.

    Genome annotation databases

    EnsembliENSMUST00000022196; ENSMUSP00000022196; ENSMUSG00000021686.
    GeneIDi11774.
    KEGGimmu:11774.
    UCSCiuc007rlv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF103809 mRNA. Translation: AAC78338.1 .
    AF255589
    , AF255566 , AF255567 , AF255568 , AF255569 , AF255570 , AF255571 , AF255572 , AF255573 , AF255574 , AF255575 , AF255576 , AF255577 , AF255578 , AF255579 , AF255580 , AF255581 , AF255582 , AF255583 , AF255584 , AF255585 , AF255586 , AF255587 , AF255588 Genomic DNA. Translation: AAG23622.1 .
    AC122373 Genomic DNA. No translation available.
    AC123043 Genomic DNA. No translation available.
    BC015068 mRNA. Translation: AAH15068.1 .
    CCDSi CCDS26693.1.
    PIRi T18295.
    RefSeqi NP_033810.2. NM_009680.3.
    UniGenei Mm.21185.

    3D structure databases

    ProteinModelPortali Q9Z1T1.
    SMRi Q9Z1T1. Positions 42-604.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198132. 4 interactions.
    DIPi DIP-49018N.
    IntActi Q9Z1T1. 6 interactions.
    MINTi MINT-1549674.

    PTM databases

    PhosphoSitei Q9Z1T1.

    Proteomic databases

    MaxQBi Q9Z1T1.
    PaxDbi Q9Z1T1.
    PRIDEi Q9Z1T1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022196 ; ENSMUSP00000022196 ; ENSMUSG00000021686 .
    GeneIDi 11774.
    KEGGi mmu:11774.
    UCSCi uc007rlv.2. mouse.

    Organism-specific databases

    CTDi 8546.
    MGIi MGI:1333879. Ap3b1.

    Phylogenomic databases

    eggNOGi COG5096.
    GeneTreei ENSGT00530000063546.
    HOGENOMi HOG000033978.
    HOVERGENi HBG050519.
    InParanoidi Q9Z1T1.
    KOi K12397.
    OMAi FVPMKTH.
    OrthoDBi EOG74R1QG.
    TreeFami TF314605.

    Miscellaneous databases

    NextBioi 279563.
    PROi Q9Z1T1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Z1T1.
    Bgeei Q9Z1T1.
    CleanExi MM_AP3B1.
    Genevestigatori Q9Z1T1.

    Family and domain databases

    Gene3Di 1.25.10.10. 2 hits.
    InterProi IPR026740. AP3_beta.
    IPR029394. AP3B1_Ser.
    IPR029390. AP3B_C.
    IPR026739. AP_beta.
    IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR002553. Clathrin/coatomer_adapt-like_N.
    [Graphical view ]
    PANTHERi PTHR11134. PTHR11134. 1 hit.
    Pfami PF01602. Adaptin_N. 1 hit.
    PF14796. AP3B1_C. 1 hit.
    PF14797. SEEEED. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037096. AP3_complex_beta. 1 hit.
    SUPFAMi SSF48371. SSF48371. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The beta-3A subunit gene (Ap3b1) of the AP-3 adaptor complex is altered in the mouse hypopigmentation mutant pearl, a model for Hermansky-Pudlak syndrome and night blindness."
      Feng L., Seymour A.B., Jiang S.Y., To A., Peden A.A., Novak E.K., Zhen L., Rusiniak M.E., Eicher E.M., Robinson M.S., Gorin M.B., Swank R.T.
      Hum. Mol. Genet. 8:323-330(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISEASE.
      Strain: C3H/HeJ.
    2. "Genomic structure of the mouse ap3b1 gene in normal and pearl mice."
      Feng L., Rigatti B.W., Novak E.K., Gorin M.B., Swank R.T.
      Genomics 69:370-379(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse."
      Larimore J., Tornieri K., Ryder P.V., Gokhale A., Zlatic S.A., Craige B., Lee J.D., Talbot K., Pare J.F., Smith Y., Faundez V.
      Mol. Biol. Cell 22:4854-4867(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ASSOCIATION WITH THE BLOC-1 COMPLEX.

    Entry informationi

    Entry nameiAP3B1_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z1T1
    Secondary accession number(s): E9QQ08, Q91YR4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3