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Q9Z1T1

- AP3B1_MOUSE

UniProt

Q9Z1T1 - AP3B1_MOUSE

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Protein

AP-3 complex subunit beta-1

Gene

Ap3b1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.1 Publication

GO - Biological processi

  1. anterograde axon cargo transport Source: UniProtKB
  2. anterograde synaptic vesicle transport Source: UniProtKB
  3. antigen processing and presentation Source: MGI
  4. antigen processing and presentation, exogenous lipid antigen via MHC class Ib Source: MGI
  5. blood coagulation Source: MGI
  6. intracellular protein transport Source: MGI
  7. melanosome organization Source: Ensembl
  8. positive regulation of NK T cell differentiation Source: MGI
  9. protein targeting to lysosome Source: MGI
  10. vesicle-mediated transport Source: MGI
  11. zinc ion transport Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_232671. Golgi Associated Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-3 complex subunit beta-1
Alternative name(s):
Adaptor protein complex AP-3 subunit beta-1
Adaptor-related protein complex 3 subunit beta-1
Beta-3A-adaptin
Clathrin assembly protein complex 3 beta-1 large chain
Gene namesi
Name:Ap3b1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:1333879. Ap3b1.

Subcellular locationi

Cytoplasmic vesicleclathrin-coated vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Golgi apparatus By similarity
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.By similarity

GO - Cellular componenti

  1. AP-3 adaptor complex Source: InterPro
  2. cytoplasmic vesicle Source: UniProtKB-KW
  3. lysosomal membrane Source: Ensembl
  4. trans-Golgi network Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Ap3b1 are the cause of the autosomal recessive phenotype 'pearl' (pe). Pearl mice exhibit hypopigmentation, lysosomal secretion abnormalities, and platelet-dense granules with reduced levels of adenine nucleotides and serotonin. The changes in platelets lead to prolonged bleeding. Additionally, pearl mice exhibit reduced sensitivity in the dark-adapted state (PubMed:9931340).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11051105AP-3 complex subunit beta-1PRO_0000193747Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei276 – 2761Phosphoserine2 Publications
Modified residuei610 – 6101PhosphoserineBy similarity
Modified residuei761 – 7611PhosphoserineBy similarity
Modified residuei763 – 7631PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z1T1.
PaxDbiQ9Z1T1.
PRIDEiQ9Z1T1.

PTM databases

PhosphoSiteiQ9Z1T1.

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiQ9Z1T1.
CleanExiMM_AP3B1.
ExpressionAtlasiQ9Z1T1. baseline and differential.
GenevestigatoriQ9Z1T1.

Interactioni

Subunit structurei

Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2). AP-3 associates with the BLOC-1 complex.

Protein-protein interaction databases

BioGridi198132. 4 interactions.
DIPiDIP-49018N.
IntActiQ9Z1T1. 6 interactions.
MINTiMINT-1549674.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1T1.
SMRiQ9Z1T1. Positions 42-636.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi678 – 802125Glu/Ser-richAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5096.
GeneTreeiENSGT00530000063546.
HOGENOMiHOG000033978.
HOVERGENiHBG050519.
InParanoidiQ9Z1T1.
KOiK12397.
OMAiFVPMKTH.
OrthoDBiEOG74R1QG.
TreeFamiTF314605.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR026740. AP3_beta.
IPR029394. AP3B1_Ser.
IPR029390. AP3B_C.
IPR026739. AP_beta.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view]
PANTHERiPTHR11134. PTHR11134. 1 hit.
PfamiPF01602. Adaptin_N. 1 hit.
PF14796. AP3B1_C. 1 hit.
PF14797. SEEEED. 1 hit.
[Graphical view]
PIRSFiPIRSF037096. AP3_complex_beta. 1 hit.
SUPFAMiSSF48371. SSF48371. 3 hits.

Sequencei

Sequence statusi: Complete.

Q9Z1T1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSNSFAYNE QSGGGEAAEL GQEATSTISP SGAFGLFSSD WKKNEDLKQM
60 70 80 90 100
LESNKDSAKL DAMKRIVGMI AKGKNASELF PAVVKNVASK NIEIKKLVYV
110 120 130 140 150
YLVRYAEEQQ DLALLSISTF QRALKDPNQL IRASALRVLS SIRVPIIVPV
160 170 180 190 200
MMLAIKEASA DLSPYVRKNA AHAIQKLYSL DPEQKEMLIE VIEKLLKDKS
210 220 230 240 250
TLVAGSVVMA FEEVCPDRID LIHRNYRKLC NLLVDVEEWG QVVIIHMLTR
260 270 280 290 300
YARTQFVSPW REDGGLEDNE KNFYESEEEE EEKEKSSRKK SYAMDPDHRL
310 320 330 340 350
LIRNTKPLLQ SRNAAVVMAV AQLYWHISPK SEAGVISKSL VRLLRSNREV
360 370 380 390 400
QYIVLQNIAT MSIERKGMFE PYLKSFYVRS TDPTMIKTLK LEILTNLANE
410 420 430 440 450
ANISTLLREF QTYVRSQDKQ FAAATIQTIG RCATSISEVT DTCLNGLVCL
460 470 480 490 500
LSNRDEIVVA ESVVVIKKLL QMQPAQHGEI IRHMAKLLDS ITVPVARASI
510 520 530 540 550
LWLIGENCER VPKIAPDVLR KMAKSFTSED DLVKLQILNL AAKLYLTNSK
560 570 580 590 600
QTKLLTQYIL NLGKYDQNYD IRDRTRFIRQ LIVPNEKSGA LSKYAKKIFL
610 620 630 640 650
APKPAPLLES PFKDRDRFQL GTLSHTLNIK ASGYLELSNW PEVAPDPSVR
660 670 680 690 700
NVEVIESAKE WTPLGKTKKE KPMKKFYSES EEEEDEDEDE DEEEEEKEDE
710 720 730 740 750
DENPSDSSSD SESGSGSESG DTGTEDSSED SSSGQDSETG SQAEAERQKV
760 770 780 790 800
AKRNSKTKRK SDSENREKKN ENSKASESSS EESSSMEDSS SESESESGSD
810 820 830 840 850
SEPAPRNVAP AKERKPQQER HPPSKDVFLL DLDDFNPVST PVALPTPALS
860 870 880 890 900
PSLIADLEGL NLSTSSSVIN VSTPVFVPTK THELLHRMHG KGLAAHYCFP
910 920 930 940 950
RQPCIFSDKM VSVQITLTNT SDRKIENIHI GGKGLPVGMQ MHAFHPIDSL
960 970 980 990 1000
EPKGSVTVSV GIDFCDSTQT ASFQLCTKDD CFNVTLQPPV GELLSPVAMS
1010 1020 1030 1040 1050
EKDFKKEQGT LTGMNETSAT LIAAPQNFTP SMILQKVVNV ANLGAVPSSQ
1060 1070 1080 1090 1100
DNVHRFAART VHSGSLMLVT VELKEGSTAQ LIINTEKTVI GSVLLRELKP

VLSQG
Length:1,105
Mass (Da):122,740
Last modified:July 27, 2011 - v2
Checksum:i59EB4A722B40E233
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti275 – 2773ESE → DS in AAH15068. (PubMed:15489334)Curated
Sequence conflicti439 – 4446VTDTCL → FTETCF in AAC78338. (PubMed:9931340)Curated
Sequence conflicti439 – 4446VTDTCL → FTETCF in AAG23622. (PubMed:11056055)Curated
Sequence conflicti487 – 4871L → F in AAC78338. (PubMed:9931340)Curated
Sequence conflicti487 – 4871L → F in AAG23622. (PubMed:11056055)Curated
Sequence conflicti691 – 6911D → DEE in AAH15068. (PubMed:15489334)Curated
Sequence conflicti714 – 7141G → E in AAH15068. (PubMed:15489334)Curated
Sequence conflicti724 – 7241T → TSN in AAH15068. (PubMed:15489334)Curated
Sequence conflicti737 – 7371S → N in AAH15068. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF103809 mRNA. Translation: AAC78338.1.
AF255589
, AF255566, AF255567, AF255568, AF255569, AF255570, AF255571, AF255572, AF255573, AF255574, AF255575, AF255576, AF255577, AF255578, AF255579, AF255580, AF255581, AF255582, AF255583, AF255584, AF255585, AF255586, AF255587, AF255588 Genomic DNA. Translation: AAG23622.1.
AC122373 Genomic DNA. No translation available.
AC123043 Genomic DNA. No translation available.
BC015068 mRNA. Translation: AAH15068.1.
CCDSiCCDS26693.1.
PIRiT18295.
RefSeqiNP_033810.2. NM_009680.3.
UniGeneiMm.21185.

Genome annotation databases

EnsembliENSMUST00000022196; ENSMUSP00000022196; ENSMUSG00000021686.
GeneIDi11774.
KEGGimmu:11774.
UCSCiuc007rlv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF103809 mRNA. Translation: AAC78338.1 .
AF255589
, AF255566 , AF255567 , AF255568 , AF255569 , AF255570 , AF255571 , AF255572 , AF255573 , AF255574 , AF255575 , AF255576 , AF255577 , AF255578 , AF255579 , AF255580 , AF255581 , AF255582 , AF255583 , AF255584 , AF255585 , AF255586 , AF255587 , AF255588 Genomic DNA. Translation: AAG23622.1 .
AC122373 Genomic DNA. No translation available.
AC123043 Genomic DNA. No translation available.
BC015068 mRNA. Translation: AAH15068.1 .
CCDSi CCDS26693.1.
PIRi T18295.
RefSeqi NP_033810.2. NM_009680.3.
UniGenei Mm.21185.

3D structure databases

ProteinModelPortali Q9Z1T1.
SMRi Q9Z1T1. Positions 42-636.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198132. 4 interactions.
DIPi DIP-49018N.
IntActi Q9Z1T1. 6 interactions.
MINTi MINT-1549674.

PTM databases

PhosphoSitei Q9Z1T1.

Proteomic databases

MaxQBi Q9Z1T1.
PaxDbi Q9Z1T1.
PRIDEi Q9Z1T1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022196 ; ENSMUSP00000022196 ; ENSMUSG00000021686 .
GeneIDi 11774.
KEGGi mmu:11774.
UCSCi uc007rlv.2. mouse.

Organism-specific databases

CTDi 8546.
MGIi MGI:1333879. Ap3b1.

Phylogenomic databases

eggNOGi COG5096.
GeneTreei ENSGT00530000063546.
HOGENOMi HOG000033978.
HOVERGENi HBG050519.
InParanoidi Q9Z1T1.
KOi K12397.
OMAi FVPMKTH.
OrthoDBi EOG74R1QG.
TreeFami TF314605.

Enzyme and pathway databases

Reactomei REACT_232671. Golgi Associated Vesicle Biogenesis.

Miscellaneous databases

NextBioi 279563.
PROi Q9Z1T1.
SOURCEi Search...

Gene expression databases

Bgeei Q9Z1T1.
CleanExi MM_AP3B1.
ExpressionAtlasi Q9Z1T1. baseline and differential.
Genevestigatori Q9Z1T1.

Family and domain databases

Gene3Di 1.25.10.10. 2 hits.
InterProi IPR026740. AP3_beta.
IPR029394. AP3B1_Ser.
IPR029390. AP3B_C.
IPR026739. AP_beta.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view ]
PANTHERi PTHR11134. PTHR11134. 1 hit.
Pfami PF01602. Adaptin_N. 1 hit.
PF14796. AP3B1_C. 1 hit.
PF14797. SEEEED. 1 hit.
[Graphical view ]
PIRSFi PIRSF037096. AP3_complex_beta. 1 hit.
SUPFAMi SSF48371. SSF48371. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The beta-3A subunit gene (Ap3b1) of the AP-3 adaptor complex is altered in the mouse hypopigmentation mutant pearl, a model for Hermansky-Pudlak syndrome and night blindness."
    Feng L., Seymour A.B., Jiang S.Y., To A., Peden A.A., Novak E.K., Zhen L., Rusiniak M.E., Eicher E.M., Robinson M.S., Gorin M.B., Swank R.T.
    Hum. Mol. Genet. 8:323-330(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISEASE.
    Strain: C3H/HeJ.
  2. "Genomic structure of the mouse ap3b1 gene in normal and pearl mice."
    Feng L., Rigatti B.W., Novak E.K., Gorin M.B., Swank R.T.
    Genomics 69:370-379(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse."
    Larimore J., Tornieri K., Ryder P.V., Gokhale A., Zlatic S.A., Craige B., Lee J.D., Talbot K., Pare J.F., Smith Y., Faundez V.
    Mol. Biol. Cell 22:4854-4867(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ASSOCIATION WITH THE BLOC-1 COMPLEX.

Entry informationi

Entry nameiAP3B1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1T1
Secondary accession number(s): E9QQ08, Q91YR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3