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Q9Z1S0 (BUB1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta

EC=2.7.11.1
Alternative name(s):
MAD3/BUB1-related protein kinase
Short name=BubR1
Mitotic checkpoint kinase MAD3L
Gene names
Name:Bub1b
Synonyms:Mad3l
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1052 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential component of the mitotic checkpoint. Required for normal mitosis progression and tumor suppression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. Essential for tumor suppression. May play a role in regulating aging and fertility By similarity. Ref.4 Ref.5

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Kinase activity stimulated by CENPE. Ref.3

Subunit structure

Interacts with CENPE, CENPF, mitosin, PLK1 and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase-promoting complex/cyclosome (APC/C). Interacts with CASC5 By similarity. Ref.3

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Chromosomecentromerekinetochore. Note: Cytoplasmic in interphase cells By similarity. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5 By similarity. Ref.3 Ref.6

Tissue specificity

Highly expressed in thymus followed by spleen.

Domain

The D-box targets the protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase Potential.

The BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.

Post-translational modification

Proteolytically cleaved by caspase-3 in a cell cycle specific manner By similarity. The cleavage might be involved in the durability of the cell cycle delay By similarity.

Acetylation at Lys-243 regulates its degradation and timing in anaphase entry By similarity.

Ubiquitinated. Degraded by the proteasome By similarity.

Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore By similarity.

Autophosphorylated in vitro By similarity. Intramolecular autophosphorylation stimulated by CENPE By similarity. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells By similarity. Phosphorylation at Ser-659 and Ser-1033 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase By similarity.

Involvement in disease

Defects in Bub1b are involved in the development of lung and intestinal adenocarcinomas after exposure to a carcinogen.

Disruption phenotype

Mice die in utero. Ref.5

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.

Contains 1 BUB1 N-terminal domain.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10521052Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
PRO_0000085674

Regions

Domain56 – 219164BUB1 N-terminal
Domain756 – 1040285Protein kinase
Nucleotide binding762 – 7709ATP By similarity
Region146 – 17934Necessary for interaction with CASC5 By similarity
Motif105 – 1128Nuclear localization signal Potential
Motif217 – 2259D-box
Compositional bias205 – 2084Poly-Glu
Compositional bias675 – 6806Poly-Ser

Sites

Active site8711Proton acceptor By similarity
Binding site7841ATP By similarity
Site572 – 5732Cleavage; by caspase-3 By similarity
Site603 – 6042Cleavage; by caspase-3 By similarity

Amino acid modifications

Modified residue2431N6-acetyllysine; by PCAF By similarity
Modified residue4281Phosphoserine By similarity
Modified residue5351Phosphoserine By similarity
Modified residue6591Phosphoserine By similarity
Modified residue6651Phosphoserine; by PLK1 By similarity
Modified residue7811Phosphothreonine; by PLK1 By similarity
Modified residue9981Phosphothreonine; by PLK1 By similarity
Modified residue10331Phosphoserine By similarity

Experimental info

Sequence conflict2751A → T in AAD11940. Ref.1
Sequence conflict2781T → P in AAD11940. Ref.1
Sequence conflict6641E → D in AAD11940. Ref.1
Sequence conflict7051D → N in AAD11940. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9Z1S0 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 9EF1ECF3735DD1F4

FASTA1,052118,392
        10         20         30         40         50         60 
MAEASEAMCL EGAEWELSKE NIQPLRHGRV MSTLQGALAK QESAGHTALQ QQKRAFESEI 

        70         80         90        100        110        120 
RFYSGDDPLD VWDRYINWTE QNYPQGGKES NMSALVERAI EALQGETRYY NDPRFLSLWI 

       130        140        150        160        170        180 
KLGHLCNEPL DMYSYLQSQG IGVSLAQFYI SWAEEYEARE NFKKADIIFQ EGIERKAEPL 

       190        200        210        220        230        240 
DRLQSQHRQF QSRVSRQAFL ALGNEEEEAL EPSEPQRSSL AELKSRGKKM ARAPISRVGG 

       250        260        270        280        290        300 
ALKAPGQSRG FLNAVPQPVH GNRRITVFDE NADTASRTEL SKPVAQPWMA PPVPRAKENE 

       310        320        330        340        350        360 
LQPGPWSTDR PAGRRPHDNP ASVTSIPSVL PSFTPYVEES AQQTVMTPCK IEPSINHVLS 

       370        380        390        400        410        420 
TRKPGREEGD PLQRVQSHQQ GCEEKKEKMM YCKEKIYAGV GEFSFEEIRA EVFRKKLKER 

       430        440        450        460        470        480 
REAELLTSAK KREEMQKQIE EMERRLKAMQ AVQQEGAGGQ QEEKMPTEDP ARLQIASGPQ 

       490        500        510        520        530        540 
EMSGVPLSCS ICPLSSNPRE ISPAENILQE QPDSKGSSMP FSIFDESLSD KKDKSPATGG 

       550        560        570        580        590        600 
PQVLNAQRRP LSVLKTTEVG TTNEDVSPDI CDELTELEPL SEDAIITGFR NVTLCPNPED 

       610        620        630        640        650        660 
TCDFARAARL ASTPFHEILS SKGIAADPEG LLQEEDLDGK AAEAHHTVHH QALIIKKLSP 

       670        680        690        700        710        720 
IIEESREATH SSGFSRSSSS APSTSSIKGF QLLEKLELTN DGAEDAIQSP WCSQYRLQLL 

       730        740        750        760        770        780 
KSLLELSAFA EFSVEDRPMP VLEIGKEIEL GPEDYVIKQE HLTCDDYRLF WVAPRSSAEL 

       790        800        810        820        830        840 
TMIKASSQPI PWDFYINLKL KERLNEDYDQ LCSCCQYQDG HVVWYQYINC STLQNLLQHS 

       850        860        870        880        890        900 
EFVTHEIIVL IIYNLLTIVE KLHRAEIVHG DLSPRSLILR NRIHDPYDYV NKDDHAVRIM 

       910        920        930        940        950        960 
DFSYSVDLRV QLDAFAYSGF RTAQILEGQK ILANCSSPYH VDLLGIADLA HLLLFKEHLH 

       970        980        990       1000       1010       1020 
VFWDGLLWKL SQSTSELKDS ELWNKFFVRI LNASDKSTVS VLGELAAEMG GAFDATFHSH 

      1030       1040       1050 
LNRALWKLGK TISPEALLTQ QDKQPGGSQS PA 

« Hide

References

« Hide 'large scale' references
[1]"The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner."
Davenport J.W., Fernandes E.R., Harris L.D., Neale G.A.M., Goorha R.
Genomics 55:113-117(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: NIH Swiss.
Tissue: Embryo.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Centromere-associated protein-E is essential for the mammalian mitotic checkpoint to prevent aneuploidy due to single chromosome loss."
Weaver B.A., Bonday Z.Q., Putkey F.R., Kops G.J., Silk A.D., Cleveland D.W.
J. Cell Biol. 162:551-563(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION, INTERACTION WITH CENPE, SUBCELLULAR LOCATION.
[4]"Slippage of mitotic arrest and enhanced tumor development in mice with BubR1 haploinsufficiency."
Dai W., Wang Q., Liu T., Swamy M., Fang Y., Xie S., Mahmood R., Yang Y.M., Xu M., Rao C.V.
Cancer Res. 64:440-445(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"BubR1 insufficiency causes early onset of aging-associated phenotypes and infertility in mice."
Baker D.J., Jeganathan K.B., Cameron J.D., Thompson M., Juneja S., Kopecka A., Kumar R., Jenkins R.B., de Groen P.C., Roche P., van Deursen J.M.
Nat. Genet. 36:744-749(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]"Bub1 maintains centromeric cohesion by activation of the spindle checkpoint."
Perera D., Tilston V., Hopwood J.A., Barchi M., Boot-Handford R.P., Taylor S.S.
Dev. Cell 13:566-579(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF107296 mRNA. Translation: AAD11940.1.
AL845470, AL929381 Genomic DNA. Translation: CAM17418.1.
AL929381, AL845470 Genomic DNA. Translation: CAM19078.1.
CCDSCCDS16579.1.
RefSeqNP_033903.2. NM_009773.3.
UniGeneMm.29133.

3D structure databases

ProteinModelPortalQ9Z1S0.
SMRQ9Z1S0. Positions 16-197, 707-1025.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198405. 33 interactions.
IntActQ9Z1S0. 29 interactions.

PTM databases

PhosphoSiteQ9Z1S0.

Proteomic databases

MaxQBQ9Z1S0.
PaxDbQ9Z1S0.
PRIDEQ9Z1S0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000038341; ENSMUSP00000037126; ENSMUSG00000040084.
GeneID12236.
KEGGmmu:12236.
UCSCuc008lse.2. mouse.

Organism-specific databases

CTD701.
MGIMGI:1333889. Bub1b.

Phylogenomic databases

eggNOGNOG317001.
GeneTreeENSGT00520000055622.
HOGENOMHOG000231849.
HOVERGENHBG050748.
InParanoidA2ARS1.
KOK06637.
OMAATHSSGF.
OrthoDBEOG7DFXCB.
TreeFamTF105456.

Enzyme and pathway databases

BRENDA2.7.11.1. 3474.

Gene expression databases

BgeeQ9Z1S0.
GenevestigatorQ9Z1S0.

Family and domain databases

InterProIPR015661. Bub1/Mad3.
IPR011009. Kinase-like_dom.
IPR013212. Mad3_BUB1_I.
IPR000719. Prot_kinase_dom.
[Graphical view]
PANTHERPTHR14030. PTHR14030. 1 hit.
PfamPF08311. Mad3_BUB1_I. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00777. Mad3_BUB1_I. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS51489. BUB1_N. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio280666.
PROQ9Z1S0.
SOURCESearch...

Entry information

Entry nameBUB1B_MOUSE
AccessionPrimary (citable) accession number: Q9Z1S0
Secondary accession number(s): A2ARS1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot