Q9Z1R3 (APOM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Apolipoprotein M
Short name=Apo-M
Short name=ApoM

Gene names

Name:	Apom
Synonyms:	Ng20

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid By similarity.
Subcellular location	Secreted Potential.
Tissue specificity	Expressed by the liver; secreted in plasma.
Sequence similarities	Belongs to the calycin superfamily. Lipocalin family. Highly divergent.

Ontologies

Keywords
Biological process	Lipid transport Transport
Cellular component	HDL Secreted
Domain	Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cholesterol efflux Inferred from mutant phenotype PubMed 15793583. Source: BHF-UCL high-density lipoprotein particle assembly Inferred from mutant phenotype PubMed 15793583. Source: BHF-UCL high-density lipoprotein particle clearance Inferred from mutant phenotype PubMed 15793583. Source: BHF-UCL high-density lipoprotein particle remodeling Inferred from mutant phenotype PubMed 15793583. Source: BHF-UCL lipoprotein metabolic process Inferred from mutant phenotype PubMed 15793583. Source: MGI negative regulation of plasma lipoprotein particle oxidation Inferred from direct assay PubMed 22204862. Source: BHF-UCL response to glucose stimulus Inferred from electronic annotation. Source: Compara reverse cholesterol transport Inferred from mutant phenotype PubMed 15793583. Source: MGI
Cellular_component	discoidal high-density lipoprotein particle Inferred from direct assay PubMed 15793583. Source: BHF-UCL low-density lipoprotein particle Inferred from electronic annotation. Source: Compara spherical high-density lipoprotein particle Inferred from electronic annotation. Source: Compara very-low-density lipoprotein particle Inferred from electronic annotation. Source: Compara
Molecular_function	antioxidant activity Inferred from direct assay PubMed 22204862. Source: BHF-UCL lipid transporter activity Inferred from electronic annotation. Source: Compara phospholipid binding Inferred from sequence or structural similarity PubMed 22204862. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 190

190

Apolipoprotein M

PRO_0000223279

Signal peptide

1 – ?22

22

Not cleaved Potential

Sites

Binding site

138

1

Fatty acid By similarity

Binding site

145

1

Fatty acid By similarity

Amino acid modifications

Disulfide bond

23 ↔ 169

By similarity

Disulfide bond

95 ↔ 185

Ref.4

Disulfide bond

130 ↔ 159

Ref.4

Secondary structure

1

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190

Helix Strand Turn

Details...

Beta strand

28 – 34

7

Beta strand

47 – 56

10

Helix

57 – 59

3

Helix

61 – 63

3

Beta strand

66 – 75

10

Beta strand

81 – 90

10

Beta strand

95 – 104

10

Helix

125 – 129

5

Beta strand

133 – 140

8

Beta strand

143 – 154

12

Helix

157 – 169

13

Beta strand

174 – 177

4

Sequences

	Sequence		Length	Mass (Da)
	Q9Z1R3 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 3D5614DADF2B77C4	FASTA	190	21,273
	10 20 30 40 50 60 MFHQVWAALL SLYGLLFNSM NQCPEHSQLT ALGMDDTETP EPHLGLWYFI AGAASTTEEL 70 80 90 100 110 120 ATFDPVDNIV FNMAAGSAPR QLQLRATIRT KSGVCVPRKW TYRLTEGKGN MELRTEGRPD 130 140 150 160 170 180 MKTDLFSSSC PGGIMLKETG QGYQRFLLYN RSPHPPEKCV EEFQSLTSCL DFKAFLVTPR 190 NQEACPLSSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Xu N., Dahlbaeck B. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse." Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L. Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 129.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo.
[4]	"Mouse ApoM displays an unprecedented seven-stranded lipocalin fold: folding decoy or alternative native fold?" Sevvana M., Kassler K., Ahnstrom J., Weiler S., Dahlback B., Sticht H., Muller Y.A. J. Mol. Biol. 404:363-371(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 20-90, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF207820 mRNA. Translation: AAF23407.1.
AF109719 Genomic DNA. Translation: AAC82478.1.
AK004530 mRNA. Translation: BAB23349.1.

IPI

IPI00130382.

RefSeq

NP_061286.1. NM_018816.1.

UniGene

Mm.2161.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2XKL	X-ray	2.50	A	20-190	[»]

ProteinModelPortal

Q9Z1R3.

SMR

Q9Z1R3. Positions 24-189.

ModBase

Search...

Proteomic databases

PaxDb

Q9Z1R3.

PRIDE

Q9Z1R3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000025249; ENSMUSP00000025249; ENSMUSG00000024391.

GeneID

55938.

KEGG

mmu:55938.

UCSC

uc008cga.1. mouse.

Organism-specific databases

CTD

55937.

MGI

MGI:1930124. Apom.

Phylogenomic databases

eggNOG

NOG40194.

GeneTree

ENSGT00390000001026.

HOGENOM

HOG000034009.

HOVERGEN

HBG018735.

InParanoid

Q9Z1R3.

OMA

NSIYQCP.

OrthoDB

EOG4TMR34.

Gene expression databases

Bgee

Q9Z1R3.

CleanEx

MM_APOM.

Genevestigator

Q9Z1R3.

GermOnline

ENSMUSG00000024391. Mus musculus.

Family and domain databases

Gene3D

2.40.128.20. 1 hit.

InterPro

IPR022734. ApoM.
IPR012674. Calycin.
IPR011038. Calycin-like.
[Graphical view]

Pfam

PF11032. ApoM. 1 hit.
[Graphical view]

SUPFAM

SSF50814. Calycin. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q9Z1R3.

NextBio

311616.

SOURCE

Search...

Entry information

Entry name

APOM_MOUSE

Accession

Primary (citable) accession number: Q9Z1R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	May 1, 1999
Last modified:	April 3, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program