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Q9Z1R2

- BAG6_MOUSE

UniProt

Q9Z1R2 - BAG6_MOUSE

Protein

Large proline-rich protein BAG6

Gene

Bag6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane By similarity. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response.By similarity
    Involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2).
    Can be released from tumor and dendritic cells in membrane vesicles or exosomes, and engage NCR3 thereby promoting natural killer cell (NK) activation and cytotoxicity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei1023 – 10242Cleavage; by caspase-3By similarity

    GO - Molecular functioni

    1. Hsp70 protein binding Source: UniProtKB
    2. polyubiquitin binding Source: UniProtKB
    3. proteasome binding Source: UniProtKB
    4. protein binding Source: UniProtKB
    5. ribosome binding Source: UniProtKB

    GO - Biological processi

    1. antigen processing and presentation of peptide antigen via MHC class I Source: UniProtKB
    2. brain development Source: UniProtKB
    3. cell differentiation Source: UniProtKB-KW
    4. chromatin modification Source: UniProtKB-KW
    5. embryo development Source: UniProtKB
    6. internal peptidyl-lysine acetylation Source: UniProtKB
    7. intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: UniProtKB
    8. intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
    9. kidney development Source: UniProtKB
    10. lung development Source: UniProtKB
    11. negative regulation of apoptotic process Source: MGI
    12. negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
    13. negative regulation of proteolysis Source: UniProtKB
    14. protein stabilization Source: UniProtKB
    15. regulation of apoptotic process Source: UniProtKB
    16. regulation of cell proliferation Source: Ensembl
    17. spermatogenesis Source: UniProtKB
    18. synaptonemal complex assembly Source: UniProtKB
    19. tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
    20. transport Source: UniProtKB-KW
    21. ubiquitin-dependent protein catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Chaperone, Chromatin regulator

    Keywords - Biological processi

    Apoptosis, Differentiation, Immunity, Spermatogenesis, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Large proline-rich protein BAG6
    Alternative name(s):
    BAG family molecular chaperone regulator 6
    BCL2-associated athanogene 6
    Short name:
    BAG-6
    Short name:
    BAG6
    HLA-B-associated transcript 3
    Protein Scythe
    Gene namesi
    Name:Bag6
    Synonyms:Bat3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1919439. Bag6.

    Subcellular locationi

    Cytoplasmcytosol 1 Publication. Nucleus By similarity
    Note: The C-terminal fragment generated by caspase-3 is cytoplasmic. Also found in extracellular vesicular exosomes in some tumor cells By similarity.By similarity

    GO - Cellular componenti

    1. BAT3 complex Source: UniProtKB
    2. cytoplasm Source: MGI
    3. cytosol Source: UniProtKB
    4. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Lethality associated with pronounced developmental defects in the lung, kidney and brain. Lethality is either embryonic consecutive to abnormal brain development or perinatal associated with pronounced developmental defects in the lung and kidney. These developmental defects were associated with widespread aberrant apoptosis and proliferation. Lethality can be partially rescued in an ICR genetic background: mice are slightly smaller in size than their wild-type counterparts and show impaired genotoxic stress responses.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11541154Large proline-rich protein BAG6PRO_0000114898Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei986 – 9861PhosphoserineBy similarity
    Modified residuei995 – 9951Phosphoserine1 Publication
    Modified residuei1103 – 11031PhosphoserineBy similarity
    Modified residuei1139 – 11391PhosphoserineBy similarity

    Post-translational modificationi

    Cleavage by caspase-3 releases a C-terminal peptide that plays a role in ricin-induced apoptosis.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9Z1R2.
    PaxDbiQ9Z1R2.
    PRIDEiQ9Z1R2.

    PTM databases

    PhosphoSiteiQ9Z1R2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9Z1R2.
    BgeeiQ9Z1R2.
    CleanExiMM_BAT3.
    GenevestigatoriQ9Z1R2.

    Interactioni

    Subunit structurei

    Component of the BAT3 complex, at least composed of BAG6/BAT3, UBL4A and GET3/TRC35. Interacts with CTCFL and p300/EP300. Interacts with ricin A chain By similarity. Interacts with AIFM1 and HSPA2.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Camk2aP11798-23EBI-644645,EBI-400402
    Tnfrsf11aO353054EBI-644645,EBI-647362

    Protein-protein interaction databases

    BioGridi230309. 4 interactions.
    IntActiQ9Z1R2. 6 interactions.
    MINTiMINT-1563095.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z1R2.
    SMRiQ9Z1R2. Positions 13-87.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini17 – 9276Ubiquitin-likePROSITE-ProRule annotationAdd
    BLAST
    Repeati237 – 271351Add
    BLAST
    Repeati416 – 444292Add
    BLAST
    Repeati597 – 624283Add
    BLAST
    Repeati630 – 658294Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni237 – 6584224 X 29 AA approximate repeatsAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi196 – 27479Pro-richAdd
    BLAST
    Compositional biasi395 – 720326Pro-richAdd
    BLAST
    Compositional biasi564 – 61047Ala-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG297129.
    GeneTreeiENSGT00390000016199.
    HOVERGENiHBG002193.
    InParanoidiQ9Z1R2.
    OMAiQEPTPGN.
    PhylomeDBiQ9Z1R2.
    TreeFamiTF328437.

    Family and domain databases

    InterProiIPR021925. DUF3538.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    [Graphical view]
    PfamiPF12057. DUF3538. 1 hit.
    PF00240. ubiquitin. 1 hit.
    [Graphical view]
    SMARTiSM00213. UBQ. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS00299. UBIQUITIN_1. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z1R2-1 [UniParc]FASTAAdd to Basket

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    MEPSDSASTA MEEPDSLEVL VKTLDSQTRT FIVGAQMNVK EFKEHIAASV     50
    SIPSEKQRLI YQGRVLQDDK KLQEYNVGGK VIHLVERAPP QTQLPSGASS 100
    GTGSASATHG GAPLPGTRGP GASVHDRNAN SYVMVGTFNL PSDGSAVDVH 150
    INMEQAPIQS EPRVRLVMAQ HMIRDIQTLL SRMECRGGTQ AQASQPPPQT 200
    PQTVASETVA LNSQTSEPVE SEAPPREPME SEEMEERPPT QTPELAPSGP 250
    APAGPAPAGP APAPETNAPN HPSPAEHVEV LQELQRLQRR LQPFLQRYCE 300
    VLGAAATTDY NNNHEGREED QRLINLVGES LRLLGNTFVA LSDLRCNLAC 350
    APPRHLHVVR PMSHYTTPMV LQQAAIPIQI NVGTTVTMTG NGARPPPAPG 400
    AEAATPGSAQ ATSLPPSSTT VDSSTEGAPP PGPAPPPASS HPRVIRISHQ 450
    SVEPVVMMHM NIQDSGAQPG GVPSAPTGPL GPPGHGQTLG QQVPGFPTAP 500
    TRVVIARPTP PQARPSHPGG PPVSGALQGA GLGTNTSLAQ MVSGLVGQLL 550
    MQPVLVAQGT PGMAQAQAQA QAQAQAQAQA PAPAPAPAPA PATASASAGT 600
    TNTATTAGPA PGGPAQPPPP QPSAADLQFS QLLGNLLGPA GPGAGGPGMA 650
    SPTITVAMPG VPAFLQGMTD FLQASQTAPP PPPPPPPPPP APEQQSTPPP 700
    GSPSGGTASP GGLGPESLPP EFFTSVVQGV LSSLLGSLGA RAGSSESIAA 750
    FIQRLSGSSN IFEPGADGAL GFFGALLSLL CQNFSMVDVV MLLHGHFQPL 800
    QRLQPQLRSF FHQHYLGGQE PTPSNIRMAT HTLITGLEEY VRESFSLVQV 850
    QPGVDIIRTN LEFLQEQFNS IAAHVLHCTD SGFGARLLEL CNQGLFECLA 900
    LNLHCLGGQQ MELAAVINGR IRRMSRGVNP SLVSWLTTMM GLRLQVVLEH 950
    MPVGPDAILR YVRRVGDPPQ TLPEEPMEVQ GAERTSPEPQ RENASPAPGT 1000
    TAEEAMSRGP PPAPEGGSRD EQDGASADAE PWAAAVPPEW VPIIQQDIQS 1050
    QRKVKPQPPL SDAYLSGMPA KRRKTMQGEG PQLLLSEAVS RAAKAAGARP 1100
    LTSPESLSRD LEAPEVQESY RQQLRSDIQK RLQEDPNYSP QRFPNAHRAF 1150
    ADDP 1154
    Length:1,154
    Mass (Da):121,037
    Last modified:May 1, 1999 - v1
    Checksum:i7F3FD14DF5AC1211
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti528 – 5281Missing in AAH26647. (PubMed:15489334)Curated
    Sequence conflicti1012 – 10121P → S in AAH26647. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF109719 Genomic DNA. Translation: AAC82479.1.
    CR974444 Genomic DNA. No translation available.
    BC026647 mRNA. Translation: AAH26647.1.
    CCDSiCCDS28688.1.
    RefSeqiNP_476512.1. NM_057171.2.
    UniGeneiMm.203962.

    Genome annotation databases

    EnsembliENSMUST00000025250; ENSMUSP00000025250; ENSMUSG00000024392.
    GeneIDi224727.
    KEGGimmu:224727.
    UCSCiuc008cgb.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF109719 Genomic DNA. Translation: AAC82479.1 .
    CR974444 Genomic DNA. No translation available.
    BC026647 mRNA. Translation: AAH26647.1 .
    CCDSi CCDS28688.1.
    RefSeqi NP_476512.1. NM_057171.2.
    UniGenei Mm.203962.

    3D structure databases

    ProteinModelPortali Q9Z1R2.
    SMRi Q9Z1R2. Positions 13-87.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 230309. 4 interactions.
    IntActi Q9Z1R2. 6 interactions.
    MINTi MINT-1563095.

    PTM databases

    PhosphoSitei Q9Z1R2.

    Proteomic databases

    MaxQBi Q9Z1R2.
    PaxDbi Q9Z1R2.
    PRIDEi Q9Z1R2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025250 ; ENSMUSP00000025250 ; ENSMUSG00000024392 .
    GeneIDi 224727.
    KEGGi mmu:224727.
    UCSCi uc008cgb.2. mouse.

    Organism-specific databases

    CTDi 7917.
    MGIi MGI:1919439. Bag6.

    Phylogenomic databases

    eggNOGi NOG297129.
    GeneTreei ENSGT00390000016199.
    HOVERGENi HBG002193.
    InParanoidi Q9Z1R2.
    OMAi QEPTPGN.
    PhylomeDBi Q9Z1R2.
    TreeFami TF328437.

    Miscellaneous databases

    NextBioi 377324.
    PROi Q9Z1R2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Z1R2.
    Bgeei Q9Z1R2.
    CleanExi MM_BAT3.
    Genevestigatori Q9Z1R2.

    Family and domain databases

    InterProi IPR021925. DUF3538.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    [Graphical view ]
    Pfami PF12057. DUF3538. 1 hit.
    PF00240. ubiquitin. 1 hit.
    [Graphical view ]
    SMARTi SM00213. UBQ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS00299. UBIQUITIN_1. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
      Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
      Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 129.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 318-1154.
      Strain: FVB/N.
      Tissue: Colon.
    4. "The reaper-binding protein scythe modulates apoptosis and proliferation during mammalian development."
      Desmots F., Russell H.R., Lee Y., Boyd K., McKinnon P.J.
      Mol. Cell. Biol. 25:10329-10337(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    5. "HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53."
      Sasaki T., Gan E.C., Wakeham A., Kornbluth S., Mak T.W., Okada H.
      Genes Dev. 21:848-861(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-995, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    7. "Scythe regulates apoptosis-inducing factor stability during endoplasmic reticulum stress-induced apoptosis."
      Desmots F., Russell H.R., Michel D., McKinnon P.J.
      J. Biol. Chem. 283:3264-3271(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH AIFM1, SUBCELLULAR LOCATION.
    8. "Bat3 deficiency accelerates the degradation of Hsp70-2/HspA2 during spermatogenesis."
      Sasaki T., Marcon E., McQuire T., Arai Y., Moens P.B., Okada H.
      J. Cell Biol. 182:449-458(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HSPA2.
    9. "BAG-6 is essential for selective elimination of defective proteasomal substrates."
      Minami R., Hayakawa A., Kagawa H., Yanagi Y., Yokosawa H., Kawahara H.
      J. Cell Biol. 190:637-650(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiBAG6_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z1R2
    Secondary accession number(s): Q8SNA3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2005
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3