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Protein

Valine--tRNA ligase

Gene

Vars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).

Enzyme regulationi

Can be regulated by protein kinase C-dependent phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei864ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Valine--tRNA ligase (EC:6.1.1.9)
Alternative name(s):
Protein G7a
Valyl-tRNA synthetase
Short name:
ValRS
Gene namesi
Name:Vars
Synonyms:Bat6, G7a, Vars2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:90675. Vars.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001062542 – 1263Valine--tRNA ligaseAdd BLAST1262

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei436PhosphoserineBy similarity1
Modified residuei526PhosphoserineBy similarity1
Modified residuei644N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Z1Q9.
MaxQBiQ9Z1Q9.
PaxDbiQ9Z1Q9.
PRIDEiQ9Z1Q9.

PTM databases

iPTMnetiQ9Z1Q9.
PhosphoSitePlusiQ9Z1Q9.
SwissPalmiQ9Z1Q9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000007029.
CleanExiMM_VARS.
MM_VARS2.
ExpressionAtlasiQ9Z1Q9. baseline and differential.
GenevisibleiQ9Z1Q9. MM.

Interactioni

Subunit structurei

Forms high-molecular-mass aggregates with elongation factor 1.By similarity

Protein-protein interaction databases

BioGridi204498. 2 interactors.
IntActiQ9Z1Q9. 3 interactors.
MINTiMINT-1869969.
STRINGi10090.ENSMUSP00000084572.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1Q9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini89 – 219GST C-terminalAdd BLAST131

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi343 – 353"HIGH" regionAdd BLAST11
Motifi861 – 865"KMSKS" region5

Sequence similaritiesi

Contains 1 GST C-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0432. Eukaryota.
KOG0867. Eukaryota.
COG0525. LUCA.
GeneTreeiENSGT00550000074727.
HOGENOMiHOG000020094.
HOVERGENiHBG017878.
InParanoidiQ9Z1Q9.
KOiK01873.
PhylomeDBiQ9Z1Q9.
TreeFamiTF300648.

Family and domain databases

CDDicd07962. Anticodon_Ia_Val. 1 hit.
Gene3Di1.10.730.10. 1 hit.
1.20.1050.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033705. Anticodon_Ia_Val.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00043. GST_C. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF47616. SSF47616. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z1Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSILYVSPHP DAFPSLRALI AARYGEAGDG PGWGGPHPRI CLQPPPSSRT
60 70 80 90 100
PFPPPRLPAL EQGPGGLWVW GAPAVAQLLW PAGLGGPGGS RAAVLVQQWV
110 120 130 140 150
SYADTELIPA ACGATLPALG LRGPGQDPQA ALGALGKALN PLEDWLRLHT
160 170 180 190 200
YLAGDAPTLA DLAAVTALLL PFRYVLDPSA RRIWGNVTRW FNTCVRQPEF
210 220 230 240 250
RAVLGEVALY SGARSVTQQP GSEVIAPQKT PAQLKKEAKK REKLEKFQQK
260 270 280 290 300
QKTQQQPPHG EKKPKPEKKE KRDPGVITYD LPTPPGEKKD VSGAMPDSYS
310 320 330 340 350
PQYVEAAWYP WWERQGFFKP EYGRPSVSAP NPRGVFMMCI PPPNVTGSLH
360 370 380 390 400
LGHALTNAIQ DSLTRWHRMR GETTLWNPGC DHAGIATQVV VEKKLWKERG
410 420 430 440 450
LNRHQLGREA FLEEVWKWKA EKGDRIYHQL KKLGSSLDWD RACFTMDPKL
460 470 480 490 500
SATVTEAFVR LHEEGVIYRS TRLVNWSCTL NSAISDIEVD KKELTGRTLL
510 520 530 540 550
PVPGYKEKVE FGVLVSFAYK VQGSDSDEEV VVATTRIETM LGDVAVAVHP
560 570 580 590 600
KDPRYQHLKG KCVVHPFLSR SLPIVFDDFV DMEFGTGAVK ITPAHDQNDY
610 620 630 640 650
EVGQRHRLEA ISIMDSKGAL INVPPPFLGL PRFEARKAVL AALKERGLFR
660 670 680 690 700
GVKDNPMVVP LCNRSKDVVE PLLRPQWYVR CGEMAQAASA AVTRGDLRIL
710 720 730 740 750
PEAHQRTWHS WMDNIRDWCI SRQLWWGHRI PAYFITVHDP AVPPGEDPDG
760 770 780 790 800
RYWVSGRTEA EAREKAAREF GVSPDKISLQ QDEDVLDTWF SSGLFPFSIF
810 820 830 840 850
GWPNQSEDLS VFYPGTLLET GHDILFFWVA RMVMLGLKLT GKLPFREVYL
860 870 880 890 900
HAIVRDAHGR KMSKSLGNVI DPLDVIHGVS LQGLYDQLLN SNLDPSEVEK
910 920 930 940 950
AKEGQKADFP AGIPECGTDA LRFGLCAYTS QGRDINLDVN RILGYRHFCN
960 970 980 990 1000
KLWNATKFAL RGLGKGFVPS ATSKPEGHES LVDRWIRSRL TEAVRLSNEG
1010 1020 1030 1040 1050
FQAYDFPAIT TAQYSFWLYE LCDVYLECLK PVLNGVDQVA AECARQTLYT
1060 1070 1080 1090 1100
CLDVGLRLLS PFMPFVTEEL FQRLPRRTPK APASLCVTPY PEPSECSWKD
1110 1120 1130 1140 1150
PEAEAALELA LSITRAVRSL RADYNLTRTR PDCFLEVADE ATGALASAVS
1160 1170 1180 1190 1200
GYVQALASAG VVAVLALGAP APQGCAVAVA SDRCSIHLQL QGLVDPAREL
1210 1220 1230 1240 1250
GKLQAKRSEA QRQAQRLQER RAASSYSAKV PLEVQEADEA KLQQTEAELR
1260
KVDEAIALFQ KML
Length:1,263
Mass (Da):140,215
Last modified:May 1, 1999 - v1
Checksum:iB510E73284FCE26D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti959A → R in AAD26532 (PubMed:10199925).Curated1
Sequence conflicti959A → R in AAD26531 (PubMed:10199925).Curated1
Sequence conflicti1219E → K in AAD26532 (PubMed:10199925).Curated1
Sequence conflicti1219E → K in AAD26531 (PubMed:10199925).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087680 mRNA. Translation: AAD26532.1.
AF087141 Genomic DNA. Translation: AAD26531.1.
AF109905 Genomic DNA. Translation: AAC84151.1.
AF109906 Genomic DNA. Translation: AAC84172.1.
CCDSiCCDS37593.1.
RefSeqiNP_035820.3. NM_011690.3.
XP_017172884.1. XM_017317395.1.
UniGeneiMm.28420.

Genome annotation databases

EnsembliENSMUST00000087315; ENSMUSP00000084572; ENSMUSG00000007029.
GeneIDi22321.
KEGGimmu:22321.
UCSCiuc008cez.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087680 mRNA. Translation: AAD26532.1.
AF087141 Genomic DNA. Translation: AAD26531.1.
AF109905 Genomic DNA. Translation: AAC84151.1.
AF109906 Genomic DNA. Translation: AAC84172.1.
CCDSiCCDS37593.1.
RefSeqiNP_035820.3. NM_011690.3.
XP_017172884.1. XM_017317395.1.
UniGeneiMm.28420.

3D structure databases

ProteinModelPortaliQ9Z1Q9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204498. 2 interactors.
IntActiQ9Z1Q9. 3 interactors.
MINTiMINT-1869969.
STRINGi10090.ENSMUSP00000084572.

PTM databases

iPTMnetiQ9Z1Q9.
PhosphoSitePlusiQ9Z1Q9.
SwissPalmiQ9Z1Q9.

Proteomic databases

EPDiQ9Z1Q9.
MaxQBiQ9Z1Q9.
PaxDbiQ9Z1Q9.
PRIDEiQ9Z1Q9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087315; ENSMUSP00000084572; ENSMUSG00000007029.
GeneIDi22321.
KEGGimmu:22321.
UCSCiuc008cez.2. mouse.

Organism-specific databases

CTDi7407.
MGIiMGI:90675. Vars.

Phylogenomic databases

eggNOGiKOG0432. Eukaryota.
KOG0867. Eukaryota.
COG0525. LUCA.
GeneTreeiENSGT00550000074727.
HOGENOMiHOG000020094.
HOVERGENiHBG017878.
InParanoidiQ9Z1Q9.
KOiK01873.
PhylomeDBiQ9Z1Q9.
TreeFamiTF300648.

Miscellaneous databases

ChiTaRSiVars. mouse.
PROiQ9Z1Q9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000007029.
CleanExiMM_VARS.
MM_VARS2.
ExpressionAtlasiQ9Z1Q9. baseline and differential.
GenevisibleiQ9Z1Q9. MM.

Family and domain databases

CDDicd07962. Anticodon_Ia_Val. 1 hit.
Gene3Di1.10.730.10. 1 hit.
1.20.1050.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033705. Anticodon_Ia_Val.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00043. GST_C. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF47616. SSF47616. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYVC_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1Q9
Secondary accession number(s): Q9QUN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.