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Protein

Chloride intracellular channel protein 1

Gene

Clic1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride intracellular channel protein 1
Alternative name(s):
Nuclear chloride ion channel 27
Short name:
NCC27
Gene namesi
Name:Clic1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2148924. Clic1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei26 – 4621Helical; Note=After insertion into the membraneSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 241240Chloride intracellular channel protein 1PRO_0000144202Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei13 – 131N6-acetyllysineBy similarity
Disulfide bondi24 ↔ 59By similarity
Modified residuei119 – 1191N6-acetyllysineBy similarity
Modified residuei131 – 1311N6-acetyllysineCombined sources
Modified residuei233 – 2331PhosphotyrosineCombined sources

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

EPDiQ9Z1Q5.
MaxQBiQ9Z1Q5.
PaxDbiQ9Z1Q5.
PRIDEiQ9Z1Q5.
TopDownProteomicsiQ9Z1Q5.

2D gel databases

REPRODUCTION-2DPAGEQ9Z1Q5.

PTM databases

iPTMnetiQ9Z1Q5.
PhosphoSiteiQ9Z1Q5.
SwissPalmiQ9Z1Q5.

Expressioni

Gene expression databases

BgeeiQ9Z1Q5.
CleanExiMM_CLIC1.
ExpressionAtlasiQ9Z1Q5. baseline and differential.
GenevisibleiQ9Z1Q5. MM.

Interactioni

Subunit structurei

Monomer. Homodimer (in vitro). Interacts with TRAPPC2. Dimerization requires a conformation change that leads to the exposure of a large hydrophobic surface. In vivo, this may lead to membrane insertion. Interacts with AKAP9 (By similarity).By similarity

Protein-protein interaction databases

BioGridi227756. 1 interaction.
IntActiQ9Z1Q5. 3 interactions.
MINTiMINT-1652031.
STRINGi10090.ENSMUSP00000007257.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1Q5.
SMRiQ9Z1Q5. Positions 22-234.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini93 – 233141GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 9089Required for insertion into the membraneBy similarityAdd
BLAST

Domaini

Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion (By similarity).By similarity

Sequence similaritiesi

Belongs to the chloride channel CLIC family.Curated
Contains 1 GST C-terminal domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410INGZ. Eukaryota.
ENOG410ZZZX. LUCA.
GeneTreeiENSGT00550000074477.
HOGENOMiHOG000231548.
HOVERGENiHBG050994.
InParanoidiQ9Z1Q5.
KOiK05021.
OMAiGFSIPEA.
OrthoDBiEOG7X3QR3.
PhylomeDBiQ9Z1Q5.
TreeFamiTF315438.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR002946. CLIC.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF13409. GST_N_2. 1 hit.
[Graphical view]
PRINTSiPR01263. INTCLCHANNEL.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00862. O-ClC. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z1Q5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEEQPQVEL FVKAGSDGAK IGNCPFSQRL FMVLWLKGVT FNVTTVDTKR
60 70 80 90 100
RTETVQKLCP GGQLPFLLYG TEVHTDTNKI EEFLEAMLCP PRYPKLAALN
110 120 130 140 150
PESNTSGLDI FAKFSAYIKN SNPALNDNLE KGLLKALKVL DNYLTSPLPE
160 170 180 190 200
EVDETSAEDE GISQRKFLDG NELTLADCNL LPKLHIVQVV CKKYRGFTIP
210 220 230 240
EAFRGVHRYL SNAYAREEFA STCPDDEEIE LAYEQVARAL K
Length:241
Mass (Da):27,013
Last modified:January 23, 2007 - v3
Checksum:i0260A9ECEDA51B1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109905 Genomic DNA. Translation: AAC84155.1.
BC004658 mRNA. Translation: AAH04658.1.
CCDSiCCDS28675.1.
RefSeqiNP_254279.1. NM_033444.2.
UniGeneiMm.29524.

Genome annotation databases

EnsembliENSMUST00000007257; ENSMUSP00000007257; ENSMUSG00000007041.
GeneIDi114584.
KEGGimmu:114584.
UCSCiuc008cfg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109905 Genomic DNA. Translation: AAC84155.1.
BC004658 mRNA. Translation: AAH04658.1.
CCDSiCCDS28675.1.
RefSeqiNP_254279.1. NM_033444.2.
UniGeneiMm.29524.

3D structure databases

ProteinModelPortaliQ9Z1Q5.
SMRiQ9Z1Q5. Positions 22-234.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi227756. 1 interaction.
IntActiQ9Z1Q5. 3 interactions.
MINTiMINT-1652031.
STRINGi10090.ENSMUSP00000007257.

PTM databases

iPTMnetiQ9Z1Q5.
PhosphoSiteiQ9Z1Q5.
SwissPalmiQ9Z1Q5.

2D gel databases

REPRODUCTION-2DPAGEQ9Z1Q5.

Proteomic databases

EPDiQ9Z1Q5.
MaxQBiQ9Z1Q5.
PaxDbiQ9Z1Q5.
PRIDEiQ9Z1Q5.
TopDownProteomicsiQ9Z1Q5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007257; ENSMUSP00000007257; ENSMUSG00000007041.
GeneIDi114584.
KEGGimmu:114584.
UCSCiuc008cfg.1. mouse.

Organism-specific databases

CTDi1192.
MGIiMGI:2148924. Clic1.

Phylogenomic databases

eggNOGiENOG410INGZ. Eukaryota.
ENOG410ZZZX. LUCA.
GeneTreeiENSGT00550000074477.
HOGENOMiHOG000231548.
HOVERGENiHBG050994.
InParanoidiQ9Z1Q5.
KOiK05021.
OMAiGFSIPEA.
OrthoDBiEOG7X3QR3.
PhylomeDBiQ9Z1Q5.
TreeFamiTF315438.

Miscellaneous databases

ChiTaRSiClic1. mouse.
NextBioi368531.
PROiQ9Z1Q5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z1Q5.
CleanExiMM_CLIC1.
ExpressionAtlasiQ9Z1Q5. baseline and differential.
GenevisibleiQ9Z1Q5. MM.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR002946. CLIC.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF13409. GST_N_2. 1 hit.
[Graphical view]
PRINTSiPR01263. INTCLCHANNEL.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00862. O-ClC. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
    Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
    Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
    Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
    J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-233, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Mast cell.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-131, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiCLIC1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1Q5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.