Reviewed, UniProtKB/Swiss-Prot Q9Z1Q5 (CLIC1_MOUSE)

Names and origin

Protein names

Recommended name:
    Chloride intracellular channel protein 1
Alternative name(s):
    Nuclear chloride ion channel 27
      Short name=NCC27

Gene names

Name:

Clic1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions By similarity.
Subunit structure	Monomer. Homodimer (in vitro). Interacts with TRAPPC2. Dimerization requires a conformation change that leads to the exposure of a large hydrophobic surface. In vivo, this may lead to membrane insertion By similarity.
Subcellular location	Nucleus By similarity. Nucleus membrane; Single-pass membrane protein By similarity. Cytoplasm By similarity. Cell membrane; Single-pass membrane protein By similarity. Note: Mostly in the nucleus including in the nuclear membrane. Small amount in the cytoplasm and the plasma membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain By similarity.
Domain	Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion By similarity.
Sequence similarities	Belongs to the chloride channel CLIC family. Contains 1 GST C-terminal domain.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Cytoplasm Membrane Nucleus
Domain	Transmembrane
Ligand	Chloride
Molecular function	Chloride channel Ionic channel Voltage-gated channel
PTM	Acetylation Disulfide bond Phosphoprotein
Gene Ontology (GO)
Cellular component	chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB membrane fraction Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW soluble fraction Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	chloride ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct voltage-gated chloride channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Pip5k1b	P70181	2	EBI-645184,EBI-645167

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 241	240	Chloride intracellular channel protein 1		PRO_0000144202
Regions
	Transmembrane	26 – 46	21	After insertion into the membrane Potential
	Domain	93 – 233	141	GST C-terminal
	Region	2 – 90	89	Required for insertion into the membrane By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	13	1	N6-acetyllysine By similarity
	Modified residue	119	1	N6-acetyllysine By similarity
	Modified residue	131	1	N6-acetyllysine By similarity
	Modified residue	135	1	N6-acetyllysine By similarity
	Modified residue	233	1	Phosphotyrosine Ref.3
	Disulfide bond	24 ↔ 59		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z1Q5-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 0260A9ECEDA51B1C	FASTA	241	27,013
	10 20 30 40 50 60 MAEEQPQVEL FVKAGSDGAK IGNCPFSQRL FMVLWLKGVT FNVTTVDTKR RTETVQKLCP 70 80 90 100 110 120 GGQLPFLLYG TEVHTDTNKI EEFLEAMLCP PRYPKLAALN PESNTSGLDI FAKFSAYIKN 130 140 150 160 170 180 SNPALNDNLE KGLLKALKVL DNYLTSPLPE EVDETSAEDE GISQRKFLDG NELTLADCNL 190 200 210 220 230 240 LPKLHIVQVV CKKYRGFTIP EAFRGVHRYL SNAYAREEFA STCPDDEEIE LAYEQVARAL K

References

[1]	"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse." Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L. Genome Res. 13:2621-2636(2003) [PubMed: 14656967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 129.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[3]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed: 17947660] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-233, MASS SPECTROMETRY. Tissue: Mast cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF109905 Genomic DNA. Translation: AAC84155.1. BC004658 mRNA. Translation: AAH04658.1.
IPI	IPI00130344.
RefSeq	NP_254279.1.
UniGene	Mm.29524
3D structure databases
SMR	Q9Z1Q5. Positions 5-240, 6-241.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Z1Q5. 1 interaction.
STRING	Q9Z1Q5.
PTM databases
PhosphoSite	Q9Z1Q5.
2-D gel databases
REPRODUCTION-2DPAGE	Q9Z1Q5.
Proteomic databases
PRIDE	Q9Z1Q5.
Genome annotation databases
Ensembl	ENSMUST00000007257; ENSMUSP00000007257; ENSMUSG00000007041; Mus musculus. [Genome view]
GeneID	114584.
KEGG	mmu:114584.
UCSC	uc008cfg.1. mouse.
Organism-specific databases
CTD	114584.
MGI	MGI:2148924. Clic1.
Phylogenomic databases
HOGENOM	Q9Z1Q5.
HOVERGEN	Q9Z1Q5.
OMA	TEYDCEL
OrthoDB	EOG9QNQFW
Gene expression databases
ArrayExpress	Q9Z1Q5.
Bgee	Q9Z1Q5.
CleanEx	MM_CLIC1.
Genevestigator	Q9Z1Q5.
GermOnline	ENSMUSG00000007041. Mus musculus.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR002946. Int_Cl_channel. IPR012336. Thioredoxin-like_fold. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PRINTS	PR01263. INTCLCHANNEL.
TIGRFAMs	TIGR00862. O-ClC. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	368531.
SOURCE	Search...

Entry information

Entry name

CLIC1_MOUSE

Accession

Primary (citable) accession number: Q9Z1Q5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)