Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Angiopoietin-related protein 4

Gene

Angptl4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity (By similarity). In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.By similarity3 Publications

GO - Molecular functioni

  • enzyme inhibitor activity Source: UniProtKB

GO - Biological processi

  • angiogenesis Source: InterPro
  • cellular response to starvation Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of endothelial cell apoptotic process Source: MGI
  • negative regulation of lipoprotein lipase activity Source: UniProtKB
  • positive regulation of lipid metabolic process Source: UniProtKB
  • protein homooligomerization Source: Ensembl
  • response to hypoxia Source: Ensembl
  • triglyceride homeostasis Source: BHF-UCL
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Angiopoietin-related protein 4
Alternative name(s):
425O18-1
Angiopoietin-like protein 4
Fasting-induced adipose factor
Hepatic fibrinogen/angiopoietin-related protein
Short name:
HFARP
Secreted protein Bk89
Gene namesi
Name:Angptl4
Synonyms:Farp, Fiaf, Ng27
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1888999. Angptl4.

Subcellular locationi

GO - Cellular componenti

  • blood microparticle Source: MGI
  • extracellular region Source: UniProtKB
  • extracellular space Source: MGI
  • proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 410387Angiopoietin-related protein 4PRO_0000009125Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence analysis
Disulfide bondi192 ↔ 220PROSITE-ProRule annotation
Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence analysis
Glycosylationi242 – 2421N-linked (GlcNAc...)Sequence analysis
Disulfide bondi345 ↔ 358PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9Z1P8.
PRIDEiQ9Z1P8.

PTM databases

PhosphoSiteiQ9Z1P8.

Expressioni

Tissue specificityi

Predominantly expressed in adipose tissue and is strongly up-regulated by fasting in white adipose tissue and liver.1 Publication

Developmental stagei

Expressed at low levels in most organs and connective tissue at E13.5. Between E15.5 and E18.5, strongest expression in brown fat.1 Publication

Inductioni

Alterations in nutrition and leptin administration are found to modulate the expression in vivo.1 Publication

Gene expression databases

BgeeiQ9Z1P8.
CleanExiMM_ANGPTL4.
ExpressionAtlasiQ9Z1P8. baseline and differential.
GenevisibleiQ9Z1P8. MM.

Interactioni

Subunit structurei

Homooligomer. The homooligomer undergoes proteolytic processing to release its carboxyl fibrinogen-like domain, which circulates as a monomer (By similarity). The homooligomer unprocessed form is able to interact with the extracellular matrix, this interaction is found to be heparin/heparan sulfate proteoglycan dependent (in competition and direct binding assays).By similarity

Protein-protein interaction databases

DIPiDIP-61311N.
STRINGi10090.ENSMUSP00000002360.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1P8.
SMRiQ9Z1P8. Positions 188-405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini183 – 405223Fibrinogen C-terminalPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili104 – 15249Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 fibrinogen C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiKOG2579. Eukaryota.
ENOG410ZYS4. LUCA.
GeneTreeiENSGT00830000128240.
HOGENOMiHOG000015386.
HOVERGENiHBG001644.
InParanoidiQ9Z1P8.
KOiK08767.
OMAiVDFNRPW.
OrthoDBiEOG7X9G60.
PhylomeDBiQ9Z1P8.
TreeFamiTF329953.

Family and domain databases

Gene3Di3.90.215.10. 2 hits.
4.10.530.10. 1 hit.
InterProiIPR028793. ANGPTL4.
IPR014716. Fibrinogen_a/b/g_C_1.
IPR014715. Fibrinogen_a/b/g_C_2.
IPR002181. Fibrinogen_a/b/g_C_dom.
IPR020837. Fibrinogen_CS.
[Graphical view]
PANTHERiPTHR19143:SF256. PTHR19143:SF256. 1 hit.
PfamiPF00147. Fibrinogen_C. 1 hit.
[Graphical view]
SMARTiSM00186. FBG. 1 hit.
[Graphical view]
SUPFAMiSSF56496. SSF56496. 1 hit.
PROSITEiPS00514. FIBRINOGEN_C_1. 1 hit.
PS51406. FIBRINOGEN_C_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z1P8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRCAPTAGAA LVLCAATAGL LSAQGRPAQP EPPRFASWDE MNLLAHGLLQ
60 70 80 90 100
LGHGLREHVE RTRGQLGALE RRMAACGNAC QGPKGKDAPF KDSEDRVPEG
110 120 130 140 150
QTPETLQSLQ TQLKAQNSKI QQLFQKVAQQ QRYLSKQNLR IQNLQSQIDL
160 170 180 190 200
LAPTHLDNGV DKTSRGKRLP KMTQLIGLTP NATHLHRPPR DCQELFQEGE
210 220 230 240 250
RHSGLFQIQP LGSPPFLVNC EMTSDGGWTV IQRRLNGSVD FNQSWEAYKD
260 270 280 290 300
GFGDPQGEFW LGLEKMHSIT GNRGSQLAVQ LQDWDGNAKL LQFPIHLGGE
310 320 330 340 350
DTAYSLQLTE PTANELGATN VSPNGLSLPF STWDQDHDLR GDLNCAKSLS
360 370 380 390 400
GGWWFGTCSH SNLNGQYFHS IPRQRQERKK GIFWKTWKGR YYPLQATTLL
410
IQPMEATAAS
Length:410
Mass (Da):45,538
Last modified:May 1, 1999 - v1
Checksum:iBCEE2259921D6D81
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti168 – 1703RLP → KLS in AAF42969 (PubMed:10866690).Curated
Sequence conflicti180 – 1801P → S in AAF42969 (PubMed:10866690).Curated
Sequence conflicti189 – 1891P → A in AAF42969 (PubMed:10866690).Curated
Sequence conflicti272 – 2721N → D in AAF86342 (PubMed:10862772).Curated
Sequence conflicti272 – 2721N → D in AAF42969 (PubMed:10866690).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF169313 mRNA. Translation: AAF62869.1.
AF278699 mRNA. Translation: AAF86342.1.
AB054540 mRNA. Translation: BAB83079.1.
AF123261 mRNA. Translation: AAF42969.1.
AK014564 mRNA. Translation: BAB29431.1.
AK132761 mRNA. Translation: BAE21342.1.
AF528162 Genomic DNA. Translation: AAO17378.1.
AF110520 Genomic DNA. Translation: AAC97965.1.
BC006611 mRNA. Translation: AAH06611.1.
BC021343 mRNA. Translation: AAH21343.1.
BC025797 mRNA. Translation: AAH25797.1.
CCDSiCCDS28629.1.
RefSeqiNP_065606.2. NM_020581.2.
UniGeneiMm.196189.

Genome annotation databases

EnsembliENSMUST00000002360; ENSMUSP00000002360; ENSMUSG00000002289.
GeneIDi57875.
KEGGimmu:57875.
UCSCiuc008bzp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF169313 mRNA. Translation: AAF62869.1.
AF278699 mRNA. Translation: AAF86342.1.
AB054540 mRNA. Translation: BAB83079.1.
AF123261 mRNA. Translation: AAF42969.1.
AK014564 mRNA. Translation: BAB29431.1.
AK132761 mRNA. Translation: BAE21342.1.
AF528162 Genomic DNA. Translation: AAO17378.1.
AF110520 Genomic DNA. Translation: AAC97965.1.
BC006611 mRNA. Translation: AAH06611.1.
BC021343 mRNA. Translation: AAH21343.1.
BC025797 mRNA. Translation: AAH25797.1.
CCDSiCCDS28629.1.
RefSeqiNP_065606.2. NM_020581.2.
UniGeneiMm.196189.

3D structure databases

ProteinModelPortaliQ9Z1P8.
SMRiQ9Z1P8. Positions 188-405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61311N.
STRINGi10090.ENSMUSP00000002360.

PTM databases

PhosphoSiteiQ9Z1P8.

Proteomic databases

PaxDbiQ9Z1P8.
PRIDEiQ9Z1P8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002360; ENSMUSP00000002360; ENSMUSG00000002289.
GeneIDi57875.
KEGGimmu:57875.
UCSCiuc008bzp.2. mouse.

Organism-specific databases

CTDi51129.
MGIiMGI:1888999. Angptl4.

Phylogenomic databases

eggNOGiKOG2579. Eukaryota.
ENOG410ZYS4. LUCA.
GeneTreeiENSGT00830000128240.
HOGENOMiHOG000015386.
HOVERGENiHBG001644.
InParanoidiQ9Z1P8.
KOiK08767.
OMAiVDFNRPW.
OrthoDBiEOG7X9G60.
PhylomeDBiQ9Z1P8.
TreeFamiTF329953.

Miscellaneous databases

NextBioi314033.
PROiQ9Z1P8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z1P8.
CleanExiMM_ANGPTL4.
ExpressionAtlasiQ9Z1P8. baseline and differential.
GenevisibleiQ9Z1P8. MM.

Family and domain databases

Gene3Di3.90.215.10. 2 hits.
4.10.530.10. 1 hit.
InterProiIPR028793. ANGPTL4.
IPR014716. Fibrinogen_a/b/g_C_1.
IPR014715. Fibrinogen_a/b/g_C_2.
IPR002181. Fibrinogen_a/b/g_C_dom.
IPR020837. Fibrinogen_CS.
[Graphical view]
PANTHERiPTHR19143:SF256. PTHR19143:SF256. 1 hit.
PfamiPF00147. Fibrinogen_C. 1 hit.
[Graphical view]
SMARTiSM00186. FBG. 1 hit.
[Graphical view]
SUPFAMiSSF56496. SSF56496. 1 hit.
PROSITEiPS00514. FIBRINOGEN_C_1. 1 hit.
PS51406. FIBRINOGEN_C_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Hepatic expression, synthesis and secretion of a novel fibrinogen/angiopoietin-related protein that prevents endothelial-cell apoptosis."
    Kim I., Kim H.-G., Kim H., Kim H.-H., Park S.K., Uhm C.-S., Lee Z.H., Koh G.Y.
    Biochem. J. 346:603-610(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Characterization of the fasting-induced adipose factor FIAF, a novel peroxisome proliferator-activated receptor target gene."
    Kersten S., Mandard S., Tan N.S., Escher P., Metzger D., Chambon P., Gonzalez F.J., Desvergne B., Wahli W.
    J. Biol. Chem. 275:28488-28493(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: White adipose tissue.
  3. "Inhibition of angiogenesis and vascular leakiness by angiopoietin-related protein 4."
    Ito Y., Oike Y., Yasunaga K., Hamada K., Miyata K., Matsumoto S., Sugano S., Tanihara H., Masuho Y., Suda T.
    Cancer Res. 63:6651-6657(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TRANSGENIC MICE.
  4. "Peroxisome proliferator-activated receptor gamma target gene encoding a novel angiopoietin-related protein associated with adipose differentiation."
    Yoon J.C., Chickering T.W., Rosen E.D., Dussault B., Qin Y., Soukas A., Friedman J.M., Holmes W.E., Spiegelman B.M.
    Mol. Cell. Biol. 20:5343-5349(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, INDUCTION, POSSIBLE FUNCTION.
    Tissue: Adipocyte.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Skin and Testis.
  6. "Gene content of the 750-kb critical region for mouse embryonic ectoderm lethal tcl-w5."
    Abe K., Yuzuriha M., Sugimoto M., Ko M.S., Brathwaite M.E., Waeltz P., Nagaraja R.
    Mamm. Genome 15:265-276(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129/Sv.
  7. "Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
    Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
    Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129.
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  9. "Angiopoietin-like protein 4 decreases blood glucose and improves glucose tolerance but induces hyperlipidemia and hepatic steatosis in mice."
    Xu A., Lam M.C., Chan K.W., Wang Y., Zhang J., Hoo R.L., Xu J.Y., Chen B., Chow W.S., Tso A.W., Lam K.S.
    Proc. Natl. Acad. Sci. U.S.A. 102:6086-6091(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Extracellular matrix-bound angiopoietin-like 4 inhibits endothelial cell adhesion, migration, and sprouting and alters actin cytoskeleton."
    Cazes A., Galaup A., Chomel C., Bignon M., Brechot N., Le Jan S., Weber H., Corvol P., Muller L., Germain S., Monnot C.
    Circ. Res. 99:1207-1215(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  11. "Angiopoietin-like 4 prevents metastasis through inhibition of vascular permeability and tumor cell motility and invasiveness."
    Galaup A., Cazes A., Le Jan S., Philippe J., Connault E., Le Coz E., Mekid H., Mir L.M., Opolon P., Corvol P., Monnot C., Germain S.
    Proc. Natl. Acad. Sci. U.S.A. 103:18721-18726(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, XENOGRAFT MODELS.

Entry informationi

Entry nameiANGL4_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1P8
Secondary accession number(s): Q78ZJ9, Q9JHX7, Q9JLX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: May 1, 1999
Last modified: February 17, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Transgenic mice that express ARP4 in the skin, driven by the keratinocyte promoter, show remarkable suppression of tumor growth within the dermal layer and decreased numbers of invading blood vessels.
In xenograft models, it inhibits both intra- and extravasation of tumor cells as well as vascular permeability leading to inhibition of metastases. Expression by tumor cells induces reorganization of the actin cytoskeleton through inhibition of actin stress fiber formation and vinculin localization at focal contacts. It might prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.