Q9Z1P3 (MCL1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Induced myeloid leukemia cell differentiation protein Mcl-1 homolog

Gene names

Name:

Mcl1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis.
Subunit structure	Interacts with BFM, BBC3 and BCL2L11 and PMAIP1 By similarity. Interacts with BAX, BOK and BIK. Interacts with unphosphorylated BAD, but not with phosphorylated BAD. Interacts with BOP By similarity. Ref.1
Subcellular location	Membrane; Single-pass membrane protein By similarity. Cytoplasm By similarity. Mitochondrion By similarity. Nucleus › nucleoplasm By similarity. Note: Cytoplasmic, associated with mitochondria.
Tissue specificity	Ubiquitous. Highly expressed in heart, spleen, lung, liver, skeletal muscle and kidney. Detected at lower levels in brain, ovary, oviduct and testis. Ref.1
Developmental stage	Detected in neonate ovaries and expressed at constant levels in 3 to 6 day old animals. Levels are increased in at day 12 and thereafter.
Post-translational modification	Cleaved by CASP3 during apoptosis, yielding a pro-apoptotic C-terminal fragment By similarity. Rapidly degraded in the absence of phosphorylation in the PEST region By similarity. Phosphorylated on several Ser and Thr residues By similarity.
Sequence similarities	Belongs to the Bcl-2 family.

Ontologies

Keywords
Biological process	Apoptosis Differentiation
Cellular component	Cytoplasm Membrane Mitochondrion Nucleus
Domain	Transmembrane Transmembrane helix
Molecular function	Developmental protein
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of apoptotic process Inferred from direct assay PubMed 9356461. Source: RGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell nucleoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Induced myeloid leukemia cell differentiation protein Mcl-1 homolog		PRO_0000143082
Regions
	Transmembrane	307 – 329	23	Helical; Potential
	Region	85 – 155	71	PEST-like By similarity
	Motif	189 – 203	15	BH3
	Motif	232 – 252	21	BH1
	Motif	284 – 299	16	BH2
Sites
	Site	107 – 108	2	Cleavage; by caspase-3 By similarity
	Site	137 – 138	2	Cleavage; by caspase-3 By similarity
Amino acid modifications
	Modified residue	101	1	Phosphoserine By similarity
	Modified residue	142	1	Phosphoserine By similarity
	Modified residue	143	1	Phosphothreonine; by MAPK By similarity
	Cross-link	116		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	174		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	177		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Experimental info
	Sequence conflict	156	1	R → H in AAD13295. Ref.1
	Sequence conflict	231	1	T → I in AAD31519. Ref.3
	Sequence conflict	316	1	A → V in AAD31519. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9Z1P3 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: C4A9DEB41897258E	FASTA	330	35,214
	10 20 30 40 50 60 MFGLRRNAVI GLNLYCGGAS LGAGGGSPAG TRLAAEEAKA RREGGGEAAL LPGARVVARP 70 80 90 100 110 120 PPVGAEDPDV TASAERRLLK SPGLLAVPPE EMAASAAAIM SPEEELDGCE PEVLSKRPAV 130 140 150 160 170 180 LPLLERVSEA AKSSGADGSL PSTPPPPEEE DDELYRQSLE IISRYLREQA TGSKDAKPLG 190 200 210 220 230 240 EAGAAGRRAL ETLRRVGDGV QRNHETAFQG MLRKLDIKNE DDVKSFSRVM THVFKDGVTN 250 260 270 280 290 300 WGRIVTLISF GAFVAKHLKS INQESCIEPL AESITDVLVR TKRDWLVKQR GWDGFVEFFH 310 320 330 VQDLEGGIRN VLLAFAGVAG VGAGLAYLIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the antiapoptotic Bcl-2 family member myeloid cell leukemia-1 (Mcl-1) and the stimulation of its message by gonadotropins in the rat ovary." Leo C.P., Hsu S.Y., Chun S.-Y., Bae H.-W., Hsueh A.J.W. Endocrinology 140:5469-5477(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH BAD; BAX; BOK; BIK AND BCL2L11, TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Ovary.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[3]	"Mcl-1 in the rat ovary." Murray J.F., Dong Y.B., Leigh A.J., Scaramuzzi R.J., Carter N.D. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 227-330. Strain: Wistar. Tissue: Ovary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF115380 mRNA. Translation: AAD13295.1. BC078835 mRNA. Translation: AAH78835.1. AF144096 mRNA. Translation: AAD31519.1.
IPI	IPI00209084.
RefSeq	NP_068618.2. NM_021846.2.
UniGene	Rn.129914. Rn.161889.
3D structure databases
ProteinModelPortal	Q9Z1P3.
SMR	Q9Z1P3. Positions 147-307.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Z1P3. 2 interactions.
PTM databases
PhosphoSite	Q9Z1P3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	60430.
KEGG	rno:60430.
Organism-specific databases
CTD	4170.
RGD	620525. Mcl1.
Phylogenomic databases
HOVERGEN	HBG003527.
KO	K02539.
Gene expression databases
Genevestigator	Q9Z1P3.
Family and domain databases
InterPro	IPR013281. Apop_reg_Mc1. IPR002475. Bcl2-like. IPR020717. Bcl2_BH1_motif_CS. IPR020726. Bcl2_BH2_motif_CS. IPR020728. Bcl2_BH3_motif_CS. IPR026298. Blc2_fam. [Graphical view]
PANTHER	PTHR11256. PTHR11256. 1 hit. PTHR11256:SF6. PTHR11256:SF6. 1 hit.
Pfam	PF00452. Bcl-2. 1 hit. [Graphical view]
PRINTS	PR01866. APOPREGMCL1. PR01862. BCL2FAMILY.
PROSITE	PS50062. BCL2_FAMILY. 1 hit. PS01080. BH1. 1 hit. PS01258. BH2. 1 hit. PS01259. BH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	612150.

Entry information

Entry name

MCL1_RAT

Accession

Primary (citable) accession number: Q9Z1P3
Secondary accession number(s): Q6AYY5, Q9R289

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	April 3, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program