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Protein

Induced myeloid leukemia cell differentiation protein Mcl-1 homolog

Gene

Mcl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis.

GO - Molecular functioni

  • BH domain binding Source: RGD
  • protein dimerization activity Source: UniProtKB
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Apoptosis, Differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Induced myeloid leukemia cell differentiation protein Mcl-1 homolog
Gene namesi
Name:Mcl1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620525. Mcl1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei307 – 329HelicalSequence analysisAdd BLAST23

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001430821 – 330Induced myeloid leukemia cell differentiation protein Mcl-1 homologAdd BLAST330

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei101PhosphoserineBy similarity1
Cross-linki116Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei139Phosphoserine; by GSK3-alpha and GSK3-betaBy similarity1
Modified residuei142PhosphoserineBy similarity1
Modified residuei143Phosphothreonine; by MAPKBy similarity1
Cross-linki174Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki177Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Cleaved by CASP3 during apoptosis, yielding a pro-apoptotic C-terminal fragment.By similarity
Rapidly degraded in the absence of phosphorylation in the PEST region.By similarity
Phosphorylated on Ser-139, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-139 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites (By similarity).By similarity
Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-139 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei107 – 108Cleavage; by caspase-3By similarity2
Sitei137 – 138Cleavage; by caspase-3By similarity2

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ9Z1P3.

PTM databases

iPTMnetiQ9Z1P3.
PhosphoSitePlusiQ9Z1P3.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in heart, spleen, lung, liver, skeletal muscle and kidney. Detected at lower levels in brain, ovary, oviduct and testis.1 Publication

Developmental stagei

Detected in neonate ovaries and expressed at constant levels in 3 to 6 day old animals. Levels are increased in at day 12 and thereafter.

Interactioni

Subunit structurei

Interacts with HIF3A (via C-terminus domain) (By similarity). Interacts with BOK, BIK, BAX, BAK1, and TPT1. Interacts with unphosphorylated BAD (PubMed:10579309). Interacts with BMF, BBC3 and PMAIP1 (By similarity). Interacts with BOP (By similarity). Interacts with BCL2L11; may sequester BCL2L11 to prevent its pro-apoptotic activity (By similarity) (PubMed:10579309).By similarity1 Publication

GO - Molecular functioni

  • BH domain binding Source: RGD
  • protein dimerization activity Source: UniProtKB
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: GO_Central

Protein-protein interaction databases

IntActiQ9Z1P3. 2 interactors.
MINTiMINT-206606.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1P3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni85 – 155PEST-likeBy similarityAdd BLAST71

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi189 – 203BH3Add BLAST15
Motifi232 – 252BH1Add BLAST21
Motifi284 – 299BH2Add BLAST16

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG003527.
InParanoidiQ9Z1P3.
KOiK02539.
PhylomeDBiQ9Z1P3.

Family and domain databases

InterProiIPR013281. Apop_reg_Mc1.
IPR002475. Bcl2-like.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
IPR020728. Bcl2_BH3_motif_CS.
IPR026298. Blc2_fam.
[Graphical view]
PANTHERiPTHR11256. PTHR11256. 1 hit.
PfamiPF00452. Bcl-2. 1 hit.
[Graphical view]
PRINTSiPR01866. APOPREGMCL1.
PR01862. BCL2FAMILY.
PROSITEiPS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
PS01259. BH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z1P3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGLRRNAVI GLNLYCGGAS LGAGGGSPAG TRLAAEEAKA RREGGGEAAL
60 70 80 90 100
LPGARVVARP PPVGAEDPDV TASAERRLLK SPGLLAVPPE EMAASAAAIM
110 120 130 140 150
SPEEELDGCE PEVLSKRPAV LPLLERVSEA AKSSGADGSL PSTPPPPEEE
160 170 180 190 200
DDELYRQSLE IISRYLREQA TGSKDAKPLG EAGAAGRRAL ETLRRVGDGV
210 220 230 240 250
QRNHETAFQG MLRKLDIKNE DDVKSFSRVM THVFKDGVTN WGRIVTLISF
260 270 280 290 300
GAFVAKHLKS INQESCIEPL AESITDVLVR TKRDWLVKQR GWDGFVEFFH
310 320 330
VQDLEGGIRN VLLAFAGVAG VGAGLAYLIR
Length:330
Mass (Da):35,214
Last modified:December 6, 2005 - v2
Checksum:iC4A9DEB41897258E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti156R → H in AAD13295 (PubMed:10579309).Curated1
Sequence conflicti231T → I in AAD31519 (Ref. 3) Curated1
Sequence conflicti316A → V in AAD31519 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF115380 mRNA. Translation: AAD13295.1.
BC078835 mRNA. Translation: AAH78835.1.
AF144096 mRNA. Translation: AAD31519.1.
RefSeqiNP_068618.2. NM_021846.2.
UniGeneiRn.129914.
Rn.161889.

Genome annotation databases

GeneIDi60430.
KEGGirno:60430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF115380 mRNA. Translation: AAD13295.1.
BC078835 mRNA. Translation: AAH78835.1.
AF144096 mRNA. Translation: AAD31519.1.
RefSeqiNP_068618.2. NM_021846.2.
UniGeneiRn.129914.
Rn.161889.

3D structure databases

ProteinModelPortaliQ9Z1P3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Z1P3. 2 interactors.
MINTiMINT-206606.

PTM databases

iPTMnetiQ9Z1P3.
PhosphoSitePlusiQ9Z1P3.

Proteomic databases

PRIDEiQ9Z1P3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi60430.
KEGGirno:60430.

Organism-specific databases

CTDi4170.
RGDi620525. Mcl1.

Phylogenomic databases

HOVERGENiHBG003527.
InParanoidiQ9Z1P3.
KOiK02539.
PhylomeDBiQ9Z1P3.

Miscellaneous databases

PROiQ9Z1P3.

Family and domain databases

InterProiIPR013281. Apop_reg_Mc1.
IPR002475. Bcl2-like.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
IPR020728. Bcl2_BH3_motif_CS.
IPR026298. Blc2_fam.
[Graphical view]
PANTHERiPTHR11256. PTHR11256. 1 hit.
PfamiPF00452. Bcl-2. 1 hit.
[Graphical view]
PRINTSiPR01866. APOPREGMCL1.
PR01862. BCL2FAMILY.
PROSITEiPS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
PS01259. BH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCL1_RAT
AccessioniPrimary (citable) accession number: Q9Z1P3
Secondary accession number(s): Q6AYY5, Q9R289
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.