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Q9Z1N2

- ORC1_MOUSE

UniProt

Q9Z1N2 - ORC1_MOUSE

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Protein

Origin recognition complex subunit 1

Gene

Orc1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931Histone H4K20me2

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi513 – 5208ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. chromatin binding Source: InterPro
  3. DNA binding Source: MGI

GO - Biological processi

  1. DNA replication Source: MGI
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_198608. G1/S-Specific Transcription.
REACT_204269. E2F-enabled inhibition of pre-replication complex formation.
REACT_206830. E2F mediated regulation of DNA replication.

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 1
Gene namesi
Name:Orc1
Synonyms:Orc1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1328337. Orc1.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nuclear origin of replication recognition complex Source: UniProtKB
  3. nucleolus Source: Ensembl
  4. nucleus Source: MGI
  5. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 840840Origin recognition complex subunit 1PRO_0000127068Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei276 – 2761PhosphoserineBy similarity
Modified residuei291 – 2911PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated during mitosis.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z1N2.
PaxDbiQ9Z1N2.
PRIDEiQ9Z1N2.

PTM databases

PhosphoSiteiQ9Z1N2.

Expressioni

Gene expression databases

BgeeiQ9Z1N2.
ExpressionAtlasiQ9Z1N2. baseline and differential.
GenevestigatoriQ9Z1N2.

Interactioni

Subunit structurei

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase (By similarity). Interacts with CDC6 and KAT7/HBO1 (By similarity). Interacts with LRWD1 predominantly during the G1 phase and with less affinity during mitosis, when phosphorylated (By similarity).By similarity

Protein-protein interaction databases

BioGridi201974. 9 interactions.
DIPiDIP-32115N.
IntActiQ9Z1N2. 7 interactions.

Structurei

Secondary structure

1
840
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi13 – 186
Beta strandi28 – 3710
Beta strandi43 – 475
Beta strandi51 – 544
Beta strandi57 – 604
Beta strandi63 – 7311
Beta strandi76 – 783
Beta strandi80 – 9011
Helixi91 – 933
Turni96 – 983
Helixi99 – 1024
Beta strandi110 – 1145
Beta strandi117 – 1193
Beta strandi122 – 1243
Helixi125 – 1273
Beta strandi128 – 1369
Beta strandi151 – 1599
Beta strandi164 – 1663

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DOVX-ray1.70A/C9-170[»]
4DOWX-ray1.95A/B9-170[»]
ProteinModelPortaliQ9Z1N2.
SMRiQ9Z1N2. Positions 13-169, 479-713.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 170127BAHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni480 – 840361Necessary and sufficient for ORC complex assemblyBy similarityAdd
BLAST

Domaini

The BAH domain mediates binding to dimethylated histone H4 'Lys-20' (H4K20me2), which is enriched at replication origins.

Sequence similaritiesi

Belongs to the ORC1 family.Curated
Contains 1 BAH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1474.
GeneTreeiENSGT00530000063498.
HOGENOMiHOG000231132.
HOVERGENiHBG007873.
InParanoidiQ9Z1N2.
KOiK02603.
OMAiIYSQHVA.
OrthoDBiEOG7QG44J.
TreeFamiTF313743.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR001025. BAH_dom.
IPR015163. Cdc6_C_dom.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF01426. BAH. 1 hit.
PF09079. Cdc6_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00439. BAH. 1 hit.
SM01074. Cdc6_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51038. BAH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z1N2 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPSYLTRQKT RQTFSWVGRP LPNRKQFQQM YREICMKIND GSEIHIKVGQ
60 70 80 90 100
FVLIQGEDNK KPYVAKLIEL FQNGAEVPPK KCARVQWFVR FLEIPVSKRH
110 120 130 140 150
LLGRSPPAQE IFWYDCSDWD NKINVETIIG PVQVVALAPE EVIPVDQKSE
160 170 180 190 200
ETLFVKLSWN KKDFAPLPPE VLAALREQED SPEWQKPLKA KIKNVKSPAR
210 220 230 240 250
NTTEQEVKGI KSNHSTSKFH QTPANIVIPN AKKSLELDGL GFTRKPNTRW
260 270 280 290 300
SKKSSCDSLD YQKTSKRRAA FSETTSPPKK PNKPREIKPS SALETRVKNG
310 320 330 340 350
QTQPFCAKSS VVLRARNPAM TTTKLGVDNT LSPIRNGLRS SVVPSGGLTP
360 370 380 390 400
VYIRRKAKEQ ETHKEPIRTS RVHRKSSLLT LKRIRQQLCL LDGDDRDQEE
410 420 430 440 450
EESVDSESEE EDEFISSLPT RNSLGQSRTR QTPSKSPQKN PKPRTPHRAT
460 470 480 490 500
PQIRDRNLAV QEPASALEEA RLRLHVSAVP DSLPCREQEF QDIYSFVESK
510 520 530 540 550
LLDGTGGCMY ISGVPGTGKT ATVHEVIRCL QQAAETDDVP PFQYVEVNGM
560 570 580 590 600
KLTEPHQVYV QILKKLTGQK ATANHAAELL AKQFCGQGSQ KETTVLLVDE
610 620 630 640 650
LDLLWTHKQD VMYNLFDWPT HKGAHLIVLT IANTMDLPER IMMNRVSSRL
660 670 680 690 700
GLTRMSFQPY SHSQLKQILV SRLRNLRAFE DDAIQLVARK VAALSGDARR
710 720 730 740 750
CLDICRRATE ICELSHLRGD SLSLVTVAHL MEAIDEMFSS SYITAIKNSS
760 770 780 790 800
VVEQSFLRAI IAEFRRSGLE EATFQQIYSQ HVALCRMEGL PYPTMSETMA
810 820 830 840
VCSRLGSCRL LLVEPSRNDL LLRVRLNVSQ NDVLFALKEE
Length:840
Mass (Da):95,103
Last modified:July 27, 2011 - v2
Checksum:i12769943A354CA8B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti230 – 2301N → S in CAA05890. (PubMed:9862484)Curated
Sequence conflicti230 – 2301N → S in AAH15073. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ003133 mRNA. Translation: CAA05890.1.
AL626783 Genomic DNA. Translation: CAM23501.1.
BC015073 mRNA. Translation: AAH15073.1.
CCDSiCCDS18453.1.
RefSeqiNP_035145.2. NM_011015.2.
UniGeneiMm.294154.

Genome annotation databases

EnsembliENSMUST00000102744; ENSMUSP00000099805; ENSMUSG00000028587.
GeneIDi18392.
KEGGimmu:18392.
UCSCiuc008ubh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ003133 mRNA. Translation: CAA05890.1 .
AL626783 Genomic DNA. Translation: CAM23501.1 .
BC015073 mRNA. Translation: AAH15073.1 .
CCDSi CCDS18453.1.
RefSeqi NP_035145.2. NM_011015.2.
UniGenei Mm.294154.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4DOV X-ray 1.70 A/C 9-170 [» ]
4DOW X-ray 1.95 A/B 9-170 [» ]
ProteinModelPortali Q9Z1N2.
SMRi Q9Z1N2. Positions 13-169, 479-713.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201974. 9 interactions.
DIPi DIP-32115N.
IntActi Q9Z1N2. 7 interactions.

PTM databases

PhosphoSitei Q9Z1N2.

Proteomic databases

MaxQBi Q9Z1N2.
PaxDbi Q9Z1N2.
PRIDEi Q9Z1N2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000102744 ; ENSMUSP00000099805 ; ENSMUSG00000028587 .
GeneIDi 18392.
KEGGi mmu:18392.
UCSCi uc008ubh.2. mouse.

Organism-specific databases

CTDi 4998.
MGIi MGI:1328337. Orc1.

Phylogenomic databases

eggNOGi COG1474.
GeneTreei ENSGT00530000063498.
HOGENOMi HOG000231132.
HOVERGENi HBG007873.
InParanoidi Q9Z1N2.
KOi K02603.
OMAi IYSQHVA.
OrthoDBi EOG7QG44J.
TreeFami TF313743.

Enzyme and pathway databases

Reactomei REACT_198608. G1/S-Specific Transcription.
REACT_204269. E2F-enabled inhibition of pre-replication complex formation.
REACT_206830. E2F mediated regulation of DNA replication.

Miscellaneous databases

NextBioi 293996.
PROi Q9Z1N2.
SOURCEi Search...

Gene expression databases

Bgeei Q9Z1N2.
ExpressionAtlasi Q9Z1N2. baseline and differential.
Genevestigatori Q9Z1N2.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR001025. BAH_dom.
IPR015163. Cdc6_C_dom.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00004. AAA. 1 hit.
PF01426. BAH. 1 hit.
PF09079. Cdc6_C. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
SM00439. BAH. 1 hit.
SM01074. Cdc6_C. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS51038. BAH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse homolog of the yeast origin recognition complex subunit ORC1 and chromosomal localization of the cognate mouse gene Orc1."
    Zisimopoulou P., Staib C., Nanda I., Schmid M., Grummt F.
    Mol. Gen. Genet. 260:295-299(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  4. "The BAH domain of ORC1 links H4K20me2 to DNA replication licensing and Meier-Gorlin syndrome."
    Kuo A.J., Song J., Cheung P., Ishibe-Murakami S., Yamazoe S., Chen J.K., Patel D.J., Gozani O.
    Nature 484:115-119(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 9-170, INTERACTION WITH H4K20ME2.

Entry informationi

Entry nameiORC1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1N2
Secondary accession number(s): A2A8R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3