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Q9Z1N2

- ORC1_MOUSE

UniProt

Q9Z1N2 - ORC1_MOUSE

Protein

Origin recognition complex subunit 1

Gene

Orc1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei93 – 931Histone H4K20me2

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi513 – 5208ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. chromatin binding Source: InterPro
    3. DNA binding Source: MGI
    4. nucleoside-triphosphatase activity Source: InterPro

    GO - Biological processi

    1. DNA replication Source: MGI

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_198608. G1/S-Specific Transcription.
    REACT_204269. E2F-enabled inhibition of pre-replication complex formation.
    REACT_206830. E2F mediated regulation of DNA replication.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Origin recognition complex subunit 1
    Gene namesi
    Name:Orc1
    Synonyms:Orc1l
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1328337. Orc1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nuclear origin of replication recognition complex Source: UniProtKB
    3. nucleolus Source: Ensembl
    4. nucleus Source: MGI
    5. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 840840Origin recognition complex subunit 1PRO_0000127068Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei276 – 2761PhosphoserineBy similarity
    Modified residuei291 – 2911PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated during mitosis.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9Z1N2.
    PaxDbiQ9Z1N2.
    PRIDEiQ9Z1N2.

    PTM databases

    PhosphoSiteiQ9Z1N2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9Z1N2.
    BgeeiQ9Z1N2.
    GenevestigatoriQ9Z1N2.

    Interactioni

    Subunit structurei

    Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase By similarity. Interacts with CDC6 and KAT7/HBO1 By similarity. Interacts with LRWD1 predominantly during the G1 phase and with less affinity during mitosis, when phosphorylated By similarity.By similarity

    Protein-protein interaction databases

    BioGridi201974. 9 interactions.
    DIPiDIP-32115N.
    IntActiQ9Z1N2. 7 interactions.

    Structurei

    Secondary structure

    1
    840
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi13 – 186
    Beta strandi28 – 3710
    Beta strandi43 – 475
    Beta strandi51 – 544
    Beta strandi57 – 604
    Beta strandi63 – 7311
    Beta strandi76 – 783
    Beta strandi80 – 9011
    Helixi91 – 933
    Turni96 – 983
    Helixi99 – 1024
    Beta strandi110 – 1145
    Beta strandi117 – 1193
    Beta strandi122 – 1243
    Helixi125 – 1273
    Beta strandi128 – 1369
    Beta strandi151 – 1599
    Beta strandi164 – 1663

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4DOVX-ray1.70A/C9-170[»]
    4DOWX-ray1.95A/B9-170[»]
    ProteinModelPortaliQ9Z1N2.
    SMRiQ9Z1N2. Positions 13-169, 479-713.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini44 – 170127BAHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni480 – 840361Necessary and sufficient for ORC complex assemblyBy similarityAdd
    BLAST

    Domaini

    The BAH domain mediates binding to dimethylated histone H4 'Lys-20' (H4K20me2), which is enriched at replication origins.

    Sequence similaritiesi

    Belongs to the ORC1 family.Curated
    Contains 1 BAH domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1474.
    GeneTreeiENSGT00530000063498.
    HOGENOMiHOG000231132.
    HOVERGENiHBG007873.
    InParanoidiA2A8R3.
    KOiK02603.
    OMAiIYSQHVA.
    OrthoDBiEOG7QG44J.
    TreeFamiTF313743.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR001025. BAH_dom.
    IPR015163. Cdc6_C_dom.
    IPR027417. P-loop_NTPase.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00004. AAA. 1 hit.
    PF01426. BAH. 1 hit.
    PF09079. Cdc6_C. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    SM00439. BAH. 1 hit.
    SM01074. Cdc6_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS51038. BAH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z1N2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSYLTRQKT RQTFSWVGRP LPNRKQFQQM YREICMKIND GSEIHIKVGQ    50
    FVLIQGEDNK KPYVAKLIEL FQNGAEVPPK KCARVQWFVR FLEIPVSKRH 100
    LLGRSPPAQE IFWYDCSDWD NKINVETIIG PVQVVALAPE EVIPVDQKSE 150
    ETLFVKLSWN KKDFAPLPPE VLAALREQED SPEWQKPLKA KIKNVKSPAR 200
    NTTEQEVKGI KSNHSTSKFH QTPANIVIPN AKKSLELDGL GFTRKPNTRW 250
    SKKSSCDSLD YQKTSKRRAA FSETTSPPKK PNKPREIKPS SALETRVKNG 300
    QTQPFCAKSS VVLRARNPAM TTTKLGVDNT LSPIRNGLRS SVVPSGGLTP 350
    VYIRRKAKEQ ETHKEPIRTS RVHRKSSLLT LKRIRQQLCL LDGDDRDQEE 400
    EESVDSESEE EDEFISSLPT RNSLGQSRTR QTPSKSPQKN PKPRTPHRAT 450
    PQIRDRNLAV QEPASALEEA RLRLHVSAVP DSLPCREQEF QDIYSFVESK 500
    LLDGTGGCMY ISGVPGTGKT ATVHEVIRCL QQAAETDDVP PFQYVEVNGM 550
    KLTEPHQVYV QILKKLTGQK ATANHAAELL AKQFCGQGSQ KETTVLLVDE 600
    LDLLWTHKQD VMYNLFDWPT HKGAHLIVLT IANTMDLPER IMMNRVSSRL 650
    GLTRMSFQPY SHSQLKQILV SRLRNLRAFE DDAIQLVARK VAALSGDARR 700
    CLDICRRATE ICELSHLRGD SLSLVTVAHL MEAIDEMFSS SYITAIKNSS 750
    VVEQSFLRAI IAEFRRSGLE EATFQQIYSQ HVALCRMEGL PYPTMSETMA 800
    VCSRLGSCRL LLVEPSRNDL LLRVRLNVSQ NDVLFALKEE 840
    Length:840
    Mass (Da):95,103
    Last modified:July 27, 2011 - v2
    Checksum:i12769943A354CA8B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti230 – 2301N → S in CAA05890. (PubMed:9862484)Curated
    Sequence conflicti230 – 2301N → S in AAH15073. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ003133 mRNA. Translation: CAA05890.1.
    AL626783 Genomic DNA. Translation: CAM23501.1.
    BC015073 mRNA. Translation: AAH15073.1.
    CCDSiCCDS18453.1.
    RefSeqiNP_035145.2. NM_011015.2.
    UniGeneiMm.294154.

    Genome annotation databases

    EnsembliENSMUST00000102744; ENSMUSP00000099805; ENSMUSG00000028587.
    GeneIDi18392.
    KEGGimmu:18392.
    UCSCiuc008ubh.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ003133 mRNA. Translation: CAA05890.1 .
    AL626783 Genomic DNA. Translation: CAM23501.1 .
    BC015073 mRNA. Translation: AAH15073.1 .
    CCDSi CCDS18453.1.
    RefSeqi NP_035145.2. NM_011015.2.
    UniGenei Mm.294154.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4DOV X-ray 1.70 A/C 9-170 [» ]
    4DOW X-ray 1.95 A/B 9-170 [» ]
    ProteinModelPortali Q9Z1N2.
    SMRi Q9Z1N2. Positions 13-169, 479-713.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201974. 9 interactions.
    DIPi DIP-32115N.
    IntActi Q9Z1N2. 7 interactions.

    PTM databases

    PhosphoSitei Q9Z1N2.

    Proteomic databases

    MaxQBi Q9Z1N2.
    PaxDbi Q9Z1N2.
    PRIDEi Q9Z1N2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102744 ; ENSMUSP00000099805 ; ENSMUSG00000028587 .
    GeneIDi 18392.
    KEGGi mmu:18392.
    UCSCi uc008ubh.2. mouse.

    Organism-specific databases

    CTDi 4998.
    MGIi MGI:1328337. Orc1.

    Phylogenomic databases

    eggNOGi COG1474.
    GeneTreei ENSGT00530000063498.
    HOGENOMi HOG000231132.
    HOVERGENi HBG007873.
    InParanoidi A2A8R3.
    KOi K02603.
    OMAi IYSQHVA.
    OrthoDBi EOG7QG44J.
    TreeFami TF313743.

    Enzyme and pathway databases

    Reactomei REACT_198608. G1/S-Specific Transcription.
    REACT_204269. E2F-enabled inhibition of pre-replication complex formation.
    REACT_206830. E2F mediated regulation of DNA replication.

    Miscellaneous databases

    NextBioi 293996.
    PROi Q9Z1N2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Z1N2.
    Bgeei Q9Z1N2.
    Genevestigatori Q9Z1N2.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR001025. BAH_dom.
    IPR015163. Cdc6_C_dom.
    IPR027417. P-loop_NTPase.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00004. AAA. 1 hit.
    PF01426. BAH. 1 hit.
    PF09079. Cdc6_C. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    SM00439. BAH. 1 hit.
    SM01074. Cdc6_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS51038. BAH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse homolog of the yeast origin recognition complex subunit ORC1 and chromosomal localization of the cognate mouse gene Orc1."
      Zisimopoulou P., Staib C., Nanda I., Schmid M., Grummt F.
      Mol. Gen. Genet. 260:295-299(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary gland.
    4. "The BAH domain of ORC1 links H4K20me2 to DNA replication licensing and Meier-Gorlin syndrome."
      Kuo A.J., Song J., Cheung P., Ishibe-Murakami S., Yamazoe S., Chen J.K., Patel D.J., Gozani O.
      Nature 484:115-119(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 9-170, INTERACTION WITH H4K20ME2.

    Entry informationi

    Entry nameiORC1_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z1N2
    Secondary accession number(s): A2A8R3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3