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Protein

Origin recognition complex subunit 1

Gene

Orc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93Histone H4K20me21

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi513 – 520ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chromatin binding Source: InterPro
  • DNA binding Source: MGI

GO - Biological processi

  • DNA replication Source: MGI
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-113510. E2F mediated regulation of DNA replication.
R-MMU-176187. Activation of ATR in response to replication stress.
R-MMU-68616. Assembly of the ORC complex at the origin of replication.
R-MMU-68689. CDC6 association with the ORC:origin complex.
R-MMU-68827. CDT1 association with the CDC6:ORC:origin complex.
R-MMU-68867. Assembly of the pre-replicative complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-68962. Activation of the pre-replicative complex.
R-MMU-69205. G1/S-Specific Transcription.
R-MMU-69298. Association of licensing factors with the pre-replicative complex.
R-MMU-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 1
Gene namesi
Name:Orc1
Synonyms:Orc1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1328337. Orc1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001270681 – 840Origin recognition complex subunit 1Add BLAST840

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei197PhosphoserineBy similarity1
Modified residuei255PhosphoserineCombined sources1
Modified residuei258PhosphoserineCombined sources1
Modified residuei276PhosphoserineBy similarity1
Modified residuei291PhosphoserineBy similarity1
Modified residuei332PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated during mitosis.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Z1N2.
MaxQBiQ9Z1N2.
PaxDbiQ9Z1N2.
PeptideAtlasiQ9Z1N2.
PRIDEiQ9Z1N2.

PTM databases

iPTMnetiQ9Z1N2.
PhosphoSitePlusiQ9Z1N2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028587.
GenevisibleiQ9Z1N2. MM.

Interactioni

Subunit structurei

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase (By similarity). Interacts with CDC6 and KAT7/HBO1 (By similarity). Interacts with LRWD1 predominantly during the G1 phase and with less affinity during mitosis, when phosphorylated (By similarity).By similarity

Protein-protein interaction databases

BioGridi201974. 10 interactors.
DIPiDIP-32115N.
IntActiQ9Z1N2. 7 interactors.
STRINGi10090.ENSMUSP00000099805.

Structurei

Secondary structure

1840
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 18Combined sources6
Beta strandi28 – 37Combined sources10
Beta strandi43 – 47Combined sources5
Beta strandi51 – 54Combined sources4
Beta strandi57 – 60Combined sources4
Beta strandi63 – 73Combined sources11
Beta strandi76 – 78Combined sources3
Beta strandi80 – 90Combined sources11
Helixi91 – 93Combined sources3
Turni96 – 98Combined sources3
Helixi99 – 102Combined sources4
Beta strandi110 – 114Combined sources5
Beta strandi117 – 119Combined sources3
Beta strandi122 – 124Combined sources3
Helixi125 – 127Combined sources3
Beta strandi128 – 136Combined sources9
Beta strandi151 – 159Combined sources9
Beta strandi164 – 166Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DOVX-ray1.70A/C9-170[»]
4DOWX-ray1.95A/B9-170[»]
ProteinModelPortaliQ9Z1N2.
SMRiQ9Z1N2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 170BAHPROSITE-ProRule annotationAdd BLAST127

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni480 – 840Necessary and sufficient for ORC complex assemblyBy similarityAdd BLAST361

Domaini

The BAH domain mediates binding to dimethylated histone H4 'Lys-20' (H4K20me2), which is enriched at replication origins.

Sequence similaritiesi

Belongs to the ORC1 family.Curated
Contains 1 BAH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1514. Eukaryota.
COG1474. LUCA.
GeneTreeiENSGT00530000063498.
HOGENOMiHOG000231132.
HOVERGENiHBG007873.
InParanoidiQ9Z1N2.
KOiK02603.
OMAiCTRGSPQ.
OrthoDBiEOG091G0BA1.
TreeFamiTF313743.

Family and domain databases

CDDicd08768. Cdc6_C. 1 hit.
Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR001025. BAH_dom.
IPR015163. Cdc6_C.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF01426. BAH. 1 hit.
PF09079. Cdc6_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00439. BAH. 1 hit.
SM01074. Cdc6_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51038. BAH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z1N2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSYLTRQKT RQTFSWVGRP LPNRKQFQQM YREICMKIND GSEIHIKVGQ
60 70 80 90 100
FVLIQGEDNK KPYVAKLIEL FQNGAEVPPK KCARVQWFVR FLEIPVSKRH
110 120 130 140 150
LLGRSPPAQE IFWYDCSDWD NKINVETIIG PVQVVALAPE EVIPVDQKSE
160 170 180 190 200
ETLFVKLSWN KKDFAPLPPE VLAALREQED SPEWQKPLKA KIKNVKSPAR
210 220 230 240 250
NTTEQEVKGI KSNHSTSKFH QTPANIVIPN AKKSLELDGL GFTRKPNTRW
260 270 280 290 300
SKKSSCDSLD YQKTSKRRAA FSETTSPPKK PNKPREIKPS SALETRVKNG
310 320 330 340 350
QTQPFCAKSS VVLRARNPAM TTTKLGVDNT LSPIRNGLRS SVVPSGGLTP
360 370 380 390 400
VYIRRKAKEQ ETHKEPIRTS RVHRKSSLLT LKRIRQQLCL LDGDDRDQEE
410 420 430 440 450
EESVDSESEE EDEFISSLPT RNSLGQSRTR QTPSKSPQKN PKPRTPHRAT
460 470 480 490 500
PQIRDRNLAV QEPASALEEA RLRLHVSAVP DSLPCREQEF QDIYSFVESK
510 520 530 540 550
LLDGTGGCMY ISGVPGTGKT ATVHEVIRCL QQAAETDDVP PFQYVEVNGM
560 570 580 590 600
KLTEPHQVYV QILKKLTGQK ATANHAAELL AKQFCGQGSQ KETTVLLVDE
610 620 630 640 650
LDLLWTHKQD VMYNLFDWPT HKGAHLIVLT IANTMDLPER IMMNRVSSRL
660 670 680 690 700
GLTRMSFQPY SHSQLKQILV SRLRNLRAFE DDAIQLVARK VAALSGDARR
710 720 730 740 750
CLDICRRATE ICELSHLRGD SLSLVTVAHL MEAIDEMFSS SYITAIKNSS
760 770 780 790 800
VVEQSFLRAI IAEFRRSGLE EATFQQIYSQ HVALCRMEGL PYPTMSETMA
810 820 830 840
VCSRLGSCRL LLVEPSRNDL LLRVRLNVSQ NDVLFALKEE
Length:840
Mass (Da):95,103
Last modified:July 27, 2011 - v2
Checksum:i12769943A354CA8B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti230N → S in CAA05890 (PubMed:9862484).Curated1
Sequence conflicti230N → S in AAH15073 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ003133 mRNA. Translation: CAA05890.1.
AL626783 Genomic DNA. Translation: CAM23501.1.
BC015073 mRNA. Translation: AAH15073.1.
CCDSiCCDS18453.1.
RefSeqiNP_035145.2. NM_011015.2.
UniGeneiMm.294154.

Genome annotation databases

EnsembliENSMUST00000102744; ENSMUSP00000099805; ENSMUSG00000028587.
GeneIDi18392.
KEGGimmu:18392.
UCSCiuc008ubh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ003133 mRNA. Translation: CAA05890.1.
AL626783 Genomic DNA. Translation: CAM23501.1.
BC015073 mRNA. Translation: AAH15073.1.
CCDSiCCDS18453.1.
RefSeqiNP_035145.2. NM_011015.2.
UniGeneiMm.294154.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DOVX-ray1.70A/C9-170[»]
4DOWX-ray1.95A/B9-170[»]
ProteinModelPortaliQ9Z1N2.
SMRiQ9Z1N2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201974. 10 interactors.
DIPiDIP-32115N.
IntActiQ9Z1N2. 7 interactors.
STRINGi10090.ENSMUSP00000099805.

PTM databases

iPTMnetiQ9Z1N2.
PhosphoSitePlusiQ9Z1N2.

Proteomic databases

EPDiQ9Z1N2.
MaxQBiQ9Z1N2.
PaxDbiQ9Z1N2.
PeptideAtlasiQ9Z1N2.
PRIDEiQ9Z1N2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102744; ENSMUSP00000099805; ENSMUSG00000028587.
GeneIDi18392.
KEGGimmu:18392.
UCSCiuc008ubh.2. mouse.

Organism-specific databases

CTDi4998.
MGIiMGI:1328337. Orc1.

Phylogenomic databases

eggNOGiKOG1514. Eukaryota.
COG1474. LUCA.
GeneTreeiENSGT00530000063498.
HOGENOMiHOG000231132.
HOVERGENiHBG007873.
InParanoidiQ9Z1N2.
KOiK02603.
OMAiCTRGSPQ.
OrthoDBiEOG091G0BA1.
TreeFamiTF313743.

Enzyme and pathway databases

ReactomeiR-MMU-113510. E2F mediated regulation of DNA replication.
R-MMU-176187. Activation of ATR in response to replication stress.
R-MMU-68616. Assembly of the ORC complex at the origin of replication.
R-MMU-68689. CDC6 association with the ORC:origin complex.
R-MMU-68827. CDT1 association with the CDC6:ORC:origin complex.
R-MMU-68867. Assembly of the pre-replicative complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-68962. Activation of the pre-replicative complex.
R-MMU-69205. G1/S-Specific Transcription.
R-MMU-69298. Association of licensing factors with the pre-replicative complex.
R-MMU-69300. Removal of licensing factors from origins.

Miscellaneous databases

PROiQ9Z1N2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028587.
GenevisibleiQ9Z1N2. MM.

Family and domain databases

CDDicd08768. Cdc6_C. 1 hit.
Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR001025. BAH_dom.
IPR015163. Cdc6_C.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF01426. BAH. 1 hit.
PF09079. Cdc6_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00439. BAH. 1 hit.
SM01074. Cdc6_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51038. BAH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiORC1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1N2
Secondary accession number(s): A2A8R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.