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Q9Z1N2 (ORC1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Origin recognition complex subunit 1
Gene names
Name:Orc1
Synonyms:Orc1l
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length840 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication By similarity.

Subunit structure

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase By similarity. Interacts with CDC6 and KAT7/HBO1 By similarity. Interacts with LRWD1 predominantly during the G1 phase and with less affinity during mitosis, when phosphorylated By similarity. Ref.4

Subcellular location

Nucleus By similarity.

Domain

The BAH domain mediates binding to dimethylated histone H4 'Lys-20' (H4K20me2), which is enriched at replication origins.

Post-translational modification

Phosphorylated during mitosis By similarity.

Sequence similarities

Belongs to the ORC1 family.

Contains 1 BAH domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 840840Origin recognition complex subunit 1
PRO_0000127068

Regions

Domain44 – 170127BAH
Nucleotide binding513 – 5208ATP Potential
Region480 – 840361Necessary and sufficient for ORC complex assembly By similarity

Sites

Binding site931Histone H4K20me2

Amino acid modifications

Modified residue2761Phosphoserine By similarity
Modified residue2911Phosphoserine By similarity

Experimental info

Sequence conflict2301N → S in CAA05890. Ref.1
Sequence conflict2301N → S in AAH15073. Ref.3

Secondary structure

................................. 840
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9Z1N2 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 12769943A354CA8B

FASTA84095,103
        10         20         30         40         50         60 
MPSYLTRQKT RQTFSWVGRP LPNRKQFQQM YREICMKIND GSEIHIKVGQ FVLIQGEDNK 

        70         80         90        100        110        120 
KPYVAKLIEL FQNGAEVPPK KCARVQWFVR FLEIPVSKRH LLGRSPPAQE IFWYDCSDWD 

       130        140        150        160        170        180 
NKINVETIIG PVQVVALAPE EVIPVDQKSE ETLFVKLSWN KKDFAPLPPE VLAALREQED 

       190        200        210        220        230        240 
SPEWQKPLKA KIKNVKSPAR NTTEQEVKGI KSNHSTSKFH QTPANIVIPN AKKSLELDGL 

       250        260        270        280        290        300 
GFTRKPNTRW SKKSSCDSLD YQKTSKRRAA FSETTSPPKK PNKPREIKPS SALETRVKNG 

       310        320        330        340        350        360 
QTQPFCAKSS VVLRARNPAM TTTKLGVDNT LSPIRNGLRS SVVPSGGLTP VYIRRKAKEQ 

       370        380        390        400        410        420 
ETHKEPIRTS RVHRKSSLLT LKRIRQQLCL LDGDDRDQEE EESVDSESEE EDEFISSLPT 

       430        440        450        460        470        480 
RNSLGQSRTR QTPSKSPQKN PKPRTPHRAT PQIRDRNLAV QEPASALEEA RLRLHVSAVP 

       490        500        510        520        530        540 
DSLPCREQEF QDIYSFVESK LLDGTGGCMY ISGVPGTGKT ATVHEVIRCL QQAAETDDVP 

       550        560        570        580        590        600 
PFQYVEVNGM KLTEPHQVYV QILKKLTGQK ATANHAAELL AKQFCGQGSQ KETTVLLVDE 

       610        620        630        640        650        660 
LDLLWTHKQD VMYNLFDWPT HKGAHLIVLT IANTMDLPER IMMNRVSSRL GLTRMSFQPY 

       670        680        690        700        710        720 
SHSQLKQILV SRLRNLRAFE DDAIQLVARK VAALSGDARR CLDICRRATE ICELSHLRGD 

       730        740        750        760        770        780 
SLSLVTVAHL MEAIDEMFSS SYITAIKNSS VVEQSFLRAI IAEFRRSGLE EATFQQIYSQ 

       790        800        810        820        830        840 
HVALCRMEGL PYPTMSETMA VCSRLGSCRL LLVEPSRNDL LLRVRLNVSQ NDVLFALKEE 

« Hide

References

« Hide 'large scale' references
[1]"Mouse homolog of the yeast origin recognition complex subunit ORC1 and chromosomal localization of the cognate mouse gene Orc1."
Zisimopoulou P., Staib C., Nanda I., Schmid M., Grummt F.
Mol. Gen. Genet. 260:295-299(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary gland.
[4]"The BAH domain of ORC1 links H4K20me2 to DNA replication licensing and Meier-Gorlin syndrome."
Kuo A.J., Song J., Cheung P., Ishibe-Murakami S., Yamazoe S., Chen J.K., Patel D.J., Gozani O.
Nature 484:115-119(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 9-170, INTERACTION WITH H4K20ME2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ003133 mRNA. Translation: CAA05890.1.
AL626783 Genomic DNA. Translation: CAM23501.1.
BC015073 mRNA. Translation: AAH15073.1.
RefSeqNP_035145.2. NM_011015.2.
UniGeneMm.294154.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4DOVX-ray1.70A/C9-170[»]
4DOWX-ray1.95A/B9-170[»]
ProteinModelPortalQ9Z1N2.
SMRQ9Z1N2. Positions 13-169, 471-840.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201974. 8 interactions.
DIPDIP-32115N.
IntActQ9Z1N2. 7 interactions.

PTM databases

PhosphoSiteQ9Z1N2.

Proteomic databases

PaxDbQ9Z1N2.
PRIDEQ9Z1N2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102744; ENSMUSP00000099805; ENSMUSG00000028587.
GeneID18392.
KEGGmmu:18392.
UCSCuc008ubh.2. mouse.

Organism-specific databases

CTD4998.
MGIMGI:1328337. Orc1.

Phylogenomic databases

eggNOGCOG1474.
GeneTreeENSGT00530000063498.
HOGENOMHOG000231132.
HOVERGENHBG007873.
InParanoidA2A8R3.
KOK02603.
OMAQANDVPP.
OrthoDBEOG7QG44J.
TreeFamTF313743.

Gene expression databases

ArrayExpressQ9Z1N2.
BgeeQ9Z1N2.
GenevestigatorQ9Z1N2.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR001025. BAH_dom.
IPR015163. Cdc6_C_dom.
IPR020793. ORC1.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERPTHR10763:SF6. PTHR10763:SF6. 1 hit.
PfamPF00004. AAA. 1 hit.
PF01426. BAH. 1 hit.
PF09079. Cdc6_C. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00439. BAH. 1 hit.
SM01074. Cdc6_C. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS51038. BAH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio293996.
PROQ9Z1N2.
SOURCESearch...

Entry information

Entry nameORC1_MOUSE
AccessionPrimary (citable) accession number: Q9Z1N2
Secondary accession number(s): A2A8R3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot