Reviewed, UniProtKB/Swiss-Prot Q9Z1N1 (F16P2_RAT)

Names and origin

Protein names

Recommended name:
    Fructose-1,6-bisphosphatase isozyme 2
      Short name=FBPase 2
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase 2

Gene names

Name:

Fbp2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Enzyme regulation	Subject to complex allosteric regulation. The enzyme can assume an active R-state, or an inactive T-state. Intermediate conformations may exist. AMP acts as allosteric inhibitor. Fructose-2,6-biphosphate acts as competitive inhibitor By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Gluconeogenesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from direct assay. Source: RGD
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from direct assay. Source: RGD magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Fructose-1,6-bisphosphatase isozyme 2		PRO_0000200507
Regions
	Nucleotide binding	18 – 22	5	AMP By similarity
	Nucleotide binding	28 – 32	5	AMP By similarity
	Nucleotide binding	113 – 114	2	AMP By similarity
	Region	122 – 125	4	Substrate binding By similarity
	Region	213 – 216	4	Substrate binding By similarity
	Region	244 – 249	6	Substrate binding By similarity
	Region	275 – 277	3	Substrate binding By similarity
Sites
	Metal binding	69	1	Magnesium 1 By similarity
	Metal binding	98	1	Magnesium 1 By similarity
	Metal binding	98	1	Magnesium 2 By similarity
	Metal binding	119	1	Magnesium 2 By similarity
	Metal binding	119	1	Magnesium 3 By similarity
	Metal binding	121	1	Magnesium 2; via carbonyl oxygen By similarity
	Metal binding	122	1	Magnesium 3 By similarity
	Metal binding	281	1	Magnesium 3 By similarity
	Binding site	141	1	AMP By similarity
	Binding site	265	1	Substrate By similarity
Amino acid modifications
	Modified residue	216	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z1N1-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 13096E40E96095D8	FASTA	339	36,887
	10 20 30 40 50 60 MTDRSPFETD MLTLTRYVME KGRQAKGTGE LTQLLNSMLT AIKAISSAVR KAGLANLYGI 70 80 90 100 110 120 AGSVNVTGDE VKKLDVLSNS LVINMLQSSY STCVLVSEEN KEAVITAKER RGKYVVCFDP 130 140 150 160 170 180 LDGSSNIDCL ASIGTIFAIY RKTTEDEPSE KDALQPGRNI VAAGYALYGS ATLVALSTGQ 190 200 210 220 230 240 GVDLFMLDPA LGEFVLVEKD IRIKKKGKIF SLNEGYAKYF DAATAEYVQK KKFPEDGSAP 250 260 270 280 290 300 YGARYVGSMV ADVHRTLVYG GIFMYPANQK SPNGKLRLLY ECNPVAYIIE QAGGMATTGT 310 320 330 QPVLDVKPES IHQRVPLILG SPEDVQEYLS CVQRNQAGR

References

[1]	"Cloning and expression of rat muscle fructose-1,6-bisphosphatase cDNA." Al-Robaiy S., Eschrich K. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ005046 mRNA. Translation: CAA06313.1.
IPI	IPI00209038.
RefSeq	NP_446168.1.
UniGene	Rn.15319
3D structure databases
SMR	Q9Z1N1. Positions 7-337.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Z1N1.
Genome annotation databases
Ensembl	ENSRNOT00000023865; ENSRNOP00000023865; ENSRNOG00000017637; Rattus norvegicus. [Genome view]
GeneID	114508.
KEGG	rno:114508.
UCSC	NM_053716. rat.
Organism-specific databases
CTD	114508.
RGD	620930. Fbp2.
Phylogenomic databases
eggNOG	roNOG08567.
HOVERGEN	Q9Z1N1.
InParanoid	Q9Z1N1.
PhylomeDB	Q9Z1N1.
Enzyme and pathway databases
BRENDA	3.1.3.11. 248.
Gene expression databases
ArrayExpress	Q9Z1N1.
Genevestigator	Q9Z1N1.
GermOnline	ENSRNOG00000017637. Rattus norvegicus.
Family and domain databases
InterPro	IPR000146. Fructose_bisphosphatase. IPR020548. Fructose_bisphosphatase_AS. [Graphical view]
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PRINTS	PR00115. F16BPHPHTASE.
PROSITE	PS00124. FBPASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	618609.

Entry information

Entry name

F16P2_RAT

Accession

Primary (citable) accession number: Q9Z1N1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	May 1, 1999
Last modified:	January 19, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)