Q9Z1M4 (KS6B2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal protein S6 kinase beta-2
Short name=S6K-beta-2
Short name=S6K2
EC=2.7.11.1

Alternative name(s):
70 kDa ribosomal protein S6 kinase 2
p70 ribosomal S6 kinase beta
Short name=p70 S6 kinase beta
Short name=p70 S6K-beta
Short name=p70 S6KB

Gene names

Name:

Rps6kb2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphorylates specifically ribosomal protein S6.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Post-translational modification	Phosphorylated and activated by MTOR. Phosphorylation by PKC within the NLS in response to mitogenic stimuli causes cytoplasmic retention.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of translational initiation Inferred from electronic annotation. Source: Compara protein kinase B signaling cascade Inferred from genetic interaction PubMed 15249583. Source: MGI
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW peptide binding Inferred from electronic annotation. Source: Compara protein kinase activity Inferred from direct assay PubMed 15249583. Source: MGI ribosomal protein S6 kinase activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	Ribosomal protein S6 kinase beta-2		PRO_0000086215
Regions
	Domain	67 – 328	262	Protein kinase
	Domain	329 – 399	71	AGC-kinase C-terminal
	Nucleotide binding	73 – 81	9	ATP By similarity
	Motif	474 – 480	7	Nuclear localization signal By similarity
	Compositional bias	411 – 485	75	Pro-rich
Sites
	Active site	194	1	Proton acceptor By similarity
	Binding site	99	1	ATP By similarity
Amino acid modifications
	Modified residue	15	1	Phosphoserine By similarity
	Modified residue	416	1	Phosphoserine Ref.3
	Modified residue	420	1	Phosphothreonine Ref.3
	Modified residue	423	1	Phosphoserine Ref.3
	Modified residue	476	1	Phosphoserine; by PKC By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z1M4 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 396929ADAB0F6CB6	FASTA	485	53,538
	10 20 30 40 50 60 MAAVFDLDLE TEEGSEGEGE PEFSPADVCP LGELRAAGLE TVGHYEEVEL TESSVNLGPE 70 80 90 100 110 120 RIGPHCFELL SVLGKGGYGK VFQVRKVQGT NLGKIYAMKV LRKAKIVCSA KDTAHTRAER 130 140 150 160 170 180 NILESVKHPF IVELAYAFQT GGKLYLILEC LSGGELFTHL EREGIFLEDT ACFYLAEITL 190 200 210 220 230 240 ALGHLHSHGI IYRDLKPENI MLSSQGHIKL TDFGLCKESI HEGAITHTFC GTIEYMAPEI 250 260 270 280 290 300 LVRTGHNRAV DWWSLGALMY DMLTGSPPFT AENRKKTMDK IIKGKLVLPP YLTPDARDLA 310 320 330 340 350 360 KKFLKRNPTQ RIGGGLGDAA DVQRHPFFRH INWDDLLARR VDPPFRPSLQ SEEDVSQFDA 370 380 390 400 410 420 RFTRQTPVDS PDDTALSESA NQAFLGFTYV APSVLDSIKE GFSFQPKLRS PRRLNSSPRT 430 440 450 460 470 480 PISPLKFSPF EGFRPSPGPP EPMEPSLPPL LPSPPSPPPT STAPLPIRPP SGTKKSKKGR GRSGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Disruption of the p70(s6k)/p85(s6k) gene reveals a small mouse phenotype and a new functional S6 kinase." Shima H., Pende M., Chen Y., Fumagalli S., Thomas G., Kozma S.C. EMBO J. 17:6649-6659(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Placenta.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-416; THR-420 AND SER-423, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ007938 mRNA. Translation: CAA07774.1. AK014412 mRNA. Translation: BAB29335.1.
IPI	IPI00463136.
RefSeq	NP_067460.1. NM_021485.2.
UniGene	Mm.271937.
3D structure databases
ProteinModelPortal	Q9Z1M4.
SMR	Q9Z1M4. Positions 61-393.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000025749.
PTM databases
PhosphoSite	Q9Z1M4.
Proteomic databases
PaxDb	Q9Z1M4.
PRIDE	Q9Z1M4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000025749; ENSMUSP00000025749; ENSMUSG00000024830. ENSMUST00000181245; ENSMUSP00000137802; ENSMUSG00000097721.
GeneID	58988.
KEGG	mmu:58988.
UCSC	uc008fzb.1. mouse.
Organism-specific databases
CTD	6199.
MGI	MGI:1927343. Rps6kb2.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00700000104132.
HOGENOM	HOG000233033.
HOVERGEN	HBG108317.
InParanoid	Q9Z1M4.
KO	K04688.
OMA	TETSVNP.
OrthoDB	EOG4Q2DFF.
Enzyme and pathway databases
BRENDA	2.7.11.1. 3474.
Gene expression databases
ArrayExpress	Q9Z1M4.
Bgee	Q9Z1M4.
Genevestigator	Q9Z1M4.
GermOnline	ENSMUSG00000024830. Mus musculus.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR011009. Kinase-like_dom. IPR017892. Pkinase_C. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR016238. Ribosomal_S6_kinase. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PIRSF	PIRSF000605. Ribsml_S6_kin_1. 1 hit.
SMART	SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	314486.
SOURCE	Search...

Entry information

Entry name

KS6B2_MOUSE

Accession

Primary (citable) accession number: Q9Z1M4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program