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Q9Z1M0

- P2RX7_MOUSE

UniProt

Q9Z1M0 - P2RX7_MOUSE

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Protein
P2X purinoceptor 7
Gene
P2rx7, P2x7
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.

GO - Molecular functioni

  1. ATP binding Source: MGI
  2. channel activity Source: MGI
  3. copper ion binding Source: Ensembl
  4. extracellular ATP-gated cation channel activity Source: MGI
  5. lipopolysaccharide binding Source: MGI
  6. magnesium ion binding Source: Ensembl
  7. protein binding Source: MGI
  8. purinergic nucleotide receptor activity Source: RefGenome
  9. receptor activity Source: MGI
  10. zinc ion binding Source: Ensembl
Complete GO annotation...

GO - Biological processi

  1. NAD transport Source: MGI
  2. T cell homeostasis Source: MGI
  3. T cell proliferation Source: MGI
  4. activation of MAPK activity Source: MGI
  5. bleb assembly Source: MGI
  6. calcium ion transport Source: MGI
  7. cation transport Source: MGI
  8. cell morphogenesis Source: MGI
  9. cell volume homeostasis Source: MGI
  10. cellular response to extracellular stimulus Source: MGI
  11. ceramide biosynthetic process Source: MGI
  12. collagen metabolic process Source: MGI
  13. cytolysis Source: MGI
  14. defense response to Gram-positive bacterium Source: MGI
  15. extrinsic apoptotic signaling pathway Source: MGI
  16. gene expression Source: MGI
  17. homeostasis of number of cells within a tissue Source: MGI
  18. inflammatory response Source: MGI
  19. membrane budding Source: MGI
  20. membrane depolarization Source: Ensembl
  21. membrane protein ectodomain proteolysis Source: MGI
  22. mitochondrion organization Source: MGI
  23. multicellular organismal protein catabolic process Source: MGI
  24. negative regulation of MAPK cascade Source: MGI
  25. negative regulation of bone resorption Source: MGI
  26. neuronal action potential Source: Ensembl
  27. phagolysosome assembly Source: MGI
  28. phospholipid transfer to membrane Source: MGI
  29. phospholipid translocation Source: MGI
  30. plasma membrane organization Source: MGI
  31. pore complex assembly Source: MGI
  32. positive regulation of MAPK cascade Source: MGI
  33. positive regulation of T cell mediated cytotoxicity Source: MGI
  34. positive regulation of apoptotic process Source: MGI
  35. positive regulation of bone mineralization Source: MGI
  36. positive regulation of catalytic activity Source: MGI
  37. positive regulation of cytokine secretion Source: MGI
  38. positive regulation of cytolysis Source: Ensembl
  39. positive regulation of cytoskeleton organization Source: Ensembl
  40. positive regulation of gamma-aminobutyric acid secretion Source: MGI
  41. positive regulation of glutamate secretion Source: MGI
  42. positive regulation of interleukin-1 alpha secretion Source: MGI
  43. positive regulation of interleukin-1 beta production Source: MGI
  44. positive regulation of interleukin-1 beta secretion Source: MGI
  45. positive regulation of interleukin-6 production Source: MGI
  46. positive regulation of lymphocyte apoptotic process Source: MGI
  47. positive regulation of mitochondrial depolarization Source: MGI
  48. positive regulation of ossification Source: MGI
  49. positive regulation of prostaglandin secretion Source: MGI
  50. positive regulation of protein phosphorylation Source: MGI
  51. positive regulation of protein secretion Source: MGI
  52. programmed cell death Source: MGI
  53. protein oligomerization Source: MGI
  54. protein phosphorylation Source: MGI
  55. protein processing Source: MGI
  56. reactive oxygen species metabolic process Source: MGI
  57. regulation of sodium ion transport Source: Ensembl
  58. release of sequestered calcium ion into cytosol Source: MGI
  59. response to ATP Source: MGI
  60. response to bacterium Source: MGI
  61. response to calcium ion Source: MGI
  62. response to drug Source: MGI
  63. response to electrical stimulus Source: MGI
  64. response to fluid shear stress Source: MGI
  65. response to lipopolysaccharide Source: MGI
  66. response to mechanical stimulus Source: MGI
  67. response to organic cyclic compound Source: MGI
  68. response to organic substance Source: MGI
  69. response to zinc ion Source: MGI
  70. sensory perception of pain Source: BHF-UCL
  71. skeletal system morphogenesis Source: MGI
  72. synaptic vesicle exocytosis Source: MGI
  73. transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiREACT_198644. The NLRP3 inflammasome.

Names & Taxonomyi

Protein namesi
Recommended name:
P2X purinoceptor 7
Short name:
P2X7
Alternative name(s):
ATP receptor
P2Z receptor
Purinergic receptor
Gene namesi
Name:P2rx7
Synonyms:P2x7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1339957. P2rx7.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2525Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei26 – 4621Helical; Name=1; Reviewed prediction
Add
BLAST
Topological domaini47 – 334288Extracellular Reviewed prediction
Add
BLAST
Transmembranei335 – 35521Helical; Name=2; Reviewed prediction
Add
BLAST
Topological domaini356 – 595240Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. bleb Source: Ensembl
  2. cell-cell junction Source: MGI
  3. cytoplasm Source: Ensembl
  4. external side of plasma membrane Source: MGI
  5. integral component of nuclear inner membrane Source: RefGenome
  6. integral component of plasma membrane Source: MGI
  7. membrane Source: MGI
  8. membrane raft Source: Ensembl
  9. neuromuscular junction Source: MGI
  10. neuronal cell body Source: MGI
  11. plasma membrane Source: MGI
  12. protein complex Source: Ensembl
  13. synapse Source: MGI
  14. terminal bouton Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi125 – 1251R → K: Abolishes calcium responses to NAD. 1 Publication
Mutagenesisi133 – 1331R → K: No effect on calcium responses to NAD. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 595595P2X purinoceptor 7
PRO_0000161561Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi74 – 741N-linked (GlcNAc...)1 Publication
Disulfide bondi119 ↔ 168 By similarity
Modified residuei125 – 1251ADP-ribosylarginine; by ART2B
Disulfide bondi129 ↔ 152 By similarity
Modified residuei133 – 1331ADP-ribosylarginine; by ART2B
Disulfide bondi135 ↔ 162 By similarity
Glycosylationi187 – 1871N-linked (GlcNAc...) Reviewed prediction
Glycosylationi202 – 2021N-linked (GlcNAc...) Reviewed prediction
Glycosylationi213 – 2131N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi216 ↔ 226 By similarity
Glycosylationi241 – 2411N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi260 ↔ 269 By similarity

Post-translational modificationi

Phosphorylation results in its inactivation By similarity.
ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.
Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface By similarity.

Keywords - PTMi

ADP-ribosylation, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9Z1M0.
PRIDEiQ9Z1M0.

PTM databases

PhosphoSiteiQ9Z1M0.

Expressioni

Gene expression databases

ArrayExpressiQ9Z1M0.
BgeeiQ9Z1M0.
GenevestigatoriQ9Z1M0.

Interactioni

Subunit structurei

Functional P2XRs are organized as homomeric and heteromeric trimers. Interacts with LAMA3, ITGB2, ACTB, ACTN4, SVIL, MPP3, HSPA1, HSPCB, HSPA8, PIK230 and PTPRB By similarity.

Protein-protein interaction databases

BioGridi202002. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1M0.
SMRiQ9Z1M0. Positions 35-355.

Family & Domainsi

Sequence similaritiesi

Belongs to the P2X receptor family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG38999.
GeneTreeiENSGT00390000016028.
HOVERGENiHBG053086.
InParanoidiQ9Z1M0.
KOiK05220.
OMAiLCPEYPT.
OrthoDBiEOG78PV92.
TreeFamiTF328633.

Family and domain databases

Gene3Di2.60.490.10. 1 hit.
InterProiIPR003050. P2X7_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]
PANTHERiPTHR10125. PTHR10125. 1 hit.
PTHR10125:SF13. PTHR10125:SF13. 1 hit.
PfamiPF00864. P2X_receptor. 1 hit.
[Graphical view]
PRINTSiPR01314. P2X7RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsiTIGR00863. P2X. 1 hit.
PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z1M0-1 [UniParc]FASTAAdd to Basket

« Hide

MPACCSWNDV LQYETNKVTR IQSTNYGTVK WVLHMIVFSY ISFALVSDKL    50
YQRKEPVISS VHTKVKGIAE VTENVTEGGV TKLGHSIFDT ADYTFPLQGN 100
SFFVMTNYVK SEGQVQTLCP EYPRRGAQCS SDRRCKKGWM DPQSKGIQTG 150
RCVPYDKTRK TCEVSAWCPT EEEKEAPRPA LLRSAENFTV LIKNNIHFPG 200
HNYTTRNILP TMNGSCTFHK TWDPQCSIFR LGDIFQEAGE NFTEVAVQGG 250
IMGIEIYWDC NLDSWSHHCR PRYSFRRLDD KNTDESFVPG YNFRYAKYYK 300
ENNVEKRTLI KAFGIRFDIL VFGTGGKFDI IQLVVYIGST LSYFGLATVC 350
IDLLINTYSS AFCRSGVYPY CKCCEPCTVN EYYYRKKCES IMEPKPTLKY 400
VSFVDEPHIR MVDQQLLGKS LQVVKGQEVP RPQMDFSDLS RLSLSLHDSP 450
LTPGQSEEIQ LLHEEVAPKS GDSPSWCQCG NCLPSRLPEQ RRALEELCCR 500
RKPGRCITTS KLFHKLVLSR DTLQLLLLYQ DPLLVLGEEA TNSRLRHRAY 550
RCYATWRFGS QDMADFAILP SCCRWRIRKE FPKTEGQYSG FKYPY 595
Length:595
Mass (Da):68,388
Last modified:July 27, 2011 - v2
Checksum:iBE007FE195AF50A4
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111L → F in CAA08853. 1 Publication
Sequence conflicti221 – 2211T → A in CAA08853. 1 Publication
Sequence conflicti283 – 2831T → M in CAA08853. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ009823 mRNA. Translation: CAA08853.1.
AC114632 Genomic DNA. No translation available.
CCDSiCCDS19652.1.
RefSeqiNP_035157.2. NM_011027.3.
UniGeneiMm.42026.

Genome annotation databases

EnsembliENSMUST00000100737; ENSMUSP00000098303; ENSMUSG00000029468.
GeneIDi18439.
KEGGimmu:18439.
UCSCiuc008zlq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ009823 mRNA. Translation: CAA08853.1 .
AC114632 Genomic DNA. No translation available.
CCDSi CCDS19652.1.
RefSeqi NP_035157.2. NM_011027.3.
UniGenei Mm.42026.

3D structure databases

ProteinModelPortali Q9Z1M0.
SMRi Q9Z1M0. Positions 35-355.
ModBasei Search...

Protein-protein interaction databases

BioGridi 202002. 1 interaction.

Chemistry

BindingDBi Q9Z1M0.
ChEMBLi CHEMBL5183.
GuidetoPHARMACOLOGYi 484.

PTM databases

PhosphoSitei Q9Z1M0.

Proteomic databases

PaxDbi Q9Z1M0.
PRIDEi Q9Z1M0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000100737 ; ENSMUSP00000098303 ; ENSMUSG00000029468 .
GeneIDi 18439.
KEGGi mmu:18439.
UCSCi uc008zlq.1. mouse.

Organism-specific databases

CTDi 5027.
MGIi MGI:1339957. P2rx7.

Phylogenomic databases

eggNOGi NOG38999.
GeneTreei ENSGT00390000016028.
HOVERGENi HBG053086.
InParanoidi Q9Z1M0.
KOi K05220.
OMAi LCPEYPT.
OrthoDBi EOG78PV92.
TreeFami TF328633.

Enzyme and pathway databases

Reactomei REACT_198644. The NLRP3 inflammasome.

Miscellaneous databases

NextBioi 294114.
PROi Q9Z1M0.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9Z1M0.
Bgeei Q9Z1M0.
Genevestigatori Q9Z1M0.

Family and domain databases

Gene3Di 2.60.490.10. 1 hit.
InterProi IPR003050. P2X7_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view ]
PANTHERi PTHR10125. PTHR10125. 1 hit.
PTHR10125:SF13. PTHR10125:SF13. 1 hit.
Pfami PF00864. P2X_receptor. 1 hit.
[Graphical view ]
PRINTSi PR01314. P2X7RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsi TIGR00863. P2X. 1 hit.
PROSITEi PS01212. P2X_RECEPTOR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and functional characterisation of the mouse P2X7 receptor."
    Chessell I.P., Simon J., Hibell A.D., Michel A.D., Barnard E.A., Humphrey P.P.
    FEBS Lett. 439:26-30(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "ADP-ribosylation at R125 gates the P2X7 ion channel by presenting a covalent ligand to its nucleotide binding site."
    Adriouch S., Bannas P., Schwarz N., Fliegert R., Guse A.H., Seman M., Haag F., Koch-Nolte F.
    FASEB J. 22:861-869(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: ADP-RIBOSYLATION AT ARG-125 AND ARG-133 BY ART2B, MUTAGENESIS OF ARG-125 AND ARG-133.
  4. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-74.

Entry informationi

Entry nameiP2RX7_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1M0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi