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Q9Z1M0

- P2RX7_MOUSE

UniProt

Q9Z1M0 - P2RX7_MOUSE

Protein

P2X purinoceptor 7

Gene

P2rx7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.

    GO - Molecular functioni

    1. ATP binding Source: MGI
    2. channel activity Source: MGI
    3. copper ion binding Source: Ensembl
    4. extracellular ATP-gated cation channel activity Source: MGI
    5. lipopolysaccharide binding Source: MGI
    6. magnesium ion binding Source: Ensembl
    7. protein binding Source: MGI
    8. purinergic nucleotide receptor activity Source: RefGenome
    9. receptor activity Source: MGI
    10. zinc ion binding Source: Ensembl

    GO - Biological processi

    1. activation of MAPK activity Source: MGI
    2. bleb assembly Source: MGI
    3. calcium ion transport Source: MGI
    4. cation transport Source: MGI
    5. cell morphogenesis Source: MGI
    6. cellular response to extracellular stimulus Source: MGI
    7. cell volume homeostasis Source: MGI
    8. ceramide biosynthetic process Source: MGI
    9. collagen metabolic process Source: MGI
    10. cytolysis Source: MGI
    11. defense response to Gram-positive bacterium Source: MGI
    12. extrinsic apoptotic signaling pathway Source: MGI
    13. gene expression Source: MGI
    14. homeostasis of number of cells within a tissue Source: MGI
    15. inflammatory response Source: MGI
    16. membrane budding Source: MGI
    17. membrane depolarization Source: Ensembl
    18. membrane protein ectodomain proteolysis Source: MGI
    19. mitochondrion organization Source: MGI
    20. multicellular organismal protein catabolic process Source: MGI
    21. NAD transport Source: MGI
    22. negative regulation of bone resorption Source: MGI
    23. negative regulation of MAPK cascade Source: MGI
    24. neuronal action potential Source: Ensembl
    25. phagolysosome assembly Source: MGI
    26. phospholipid transfer to membrane Source: MGI
    27. phospholipid translocation Source: MGI
    28. plasma membrane organization Source: MGI
    29. pore complex assembly Source: MGI
    30. positive regulation of apoptotic process Source: MGI
    31. positive regulation of bone mineralization Source: MGI
    32. positive regulation of catalytic activity Source: MGI
    33. positive regulation of cytokine secretion Source: MGI
    34. positive regulation of cytolysis Source: Ensembl
    35. positive regulation of cytoskeleton organization Source: Ensembl
    36. positive regulation of gamma-aminobutyric acid secretion Source: MGI
    37. positive regulation of glutamate secretion Source: MGI
    38. positive regulation of interleukin-1 alpha secretion Source: MGI
    39. positive regulation of interleukin-1 beta production Source: MGI
    40. positive regulation of interleukin-1 beta secretion Source: MGI
    41. positive regulation of interleukin-6 production Source: MGI
    42. positive regulation of lymphocyte apoptotic process Source: MGI
    43. positive regulation of MAPK cascade Source: MGI
    44. positive regulation of mitochondrial depolarization Source: MGI
    45. positive regulation of ossification Source: MGI
    46. positive regulation of prostaglandin secretion Source: MGI
    47. positive regulation of protein phosphorylation Source: MGI
    48. positive regulation of protein secretion Source: MGI
    49. positive regulation of T cell mediated cytotoxicity Source: MGI
    50. programmed cell death Source: MGI
    51. protein oligomerization Source: MGI
    52. protein phosphorylation Source: MGI
    53. protein processing Source: MGI
    54. reactive oxygen species metabolic process Source: MGI
    55. regulation of sodium ion transport Source: Ensembl
    56. release of sequestered calcium ion into cytosol Source: MGI
    57. response to ATP Source: MGI
    58. response to bacterium Source: MGI
    59. response to calcium ion Source: MGI
    60. response to drug Source: MGI
    61. response to electrical stimulus Source: MGI
    62. response to fluid shear stress Source: MGI
    63. response to lipopolysaccharide Source: MGI
    64. response to mechanical stimulus Source: MGI
    65. response to organic cyclic compound Source: MGI
    66. response to organic substance Source: MGI
    67. response to zinc ion Source: MGI
    68. sensory perception of pain Source: BHF-UCL
    69. skeletal system morphogenesis Source: MGI
    70. synaptic vesicle exocytosis Source: MGI
    71. T cell homeostasis Source: MGI
    72. T cell proliferation Source: MGI
    73. transmembrane transport Source: GOC

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_198644. The NLRP3 inflammasome.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    P2X purinoceptor 7
    Short name:
    P2X7
    Alternative name(s):
    ATP receptor
    P2Z receptor
    Purinergic receptor
    Gene namesi
    Name:P2rx7
    Synonyms:P2x7
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1339957. P2rx7.

    Subcellular locationi

    GO - Cellular componenti

    1. bleb Source: Ensembl
    2. cell-cell junction Source: MGI
    3. cytoplasm Source: Ensembl
    4. external side of plasma membrane Source: MGI
    5. integral component of nuclear inner membrane Source: RefGenome
    6. integral component of plasma membrane Source: MGI
    7. membrane Source: MGI
    8. membrane raft Source: Ensembl
    9. neuromuscular junction Source: MGI
    10. neuronal cell body Source: MGI
    11. plasma membrane Source: MGI
    12. protein complex Source: Ensembl
    13. synapse Source: MGI
    14. terminal bouton Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi125 – 1251R → K: Abolishes calcium responses to NAD. 1 Publication
    Mutagenesisi133 – 1331R → K: No effect on calcium responses to NAD. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 595595P2X purinoceptor 7PRO_0000161561Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi74 – 741N-linked (GlcNAc...)1 Publication
    Disulfide bondi119 ↔ 168By similarity
    Modified residuei125 – 1251ADP-ribosylarginine; by ART2B1 Publication
    Disulfide bondi129 ↔ 152By similarity
    Modified residuei133 – 1331ADP-ribosylarginine; by ART2B1 Publication
    Disulfide bondi135 ↔ 162By similarity
    Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi202 – 2021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi216 ↔ 226By similarity
    Glycosylationi241 – 2411N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi260 ↔ 269By similarity

    Post-translational modificationi

    Phosphorylation results in its inactivation.By similarity
    ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.
    Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.By similarity

    Keywords - PTMi

    ADP-ribosylation, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiQ9Z1M0.
    PRIDEiQ9Z1M0.

    PTM databases

    PhosphoSiteiQ9Z1M0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9Z1M0.
    BgeeiQ9Z1M0.
    GenevestigatoriQ9Z1M0.

    Interactioni

    Subunit structurei

    Functional P2XRs are organized as homomeric and heteromeric trimers. Interacts with LAMA3, ITGB2, ACTB, ACTN4, SVIL, MPP3, HSPA1, HSPCB, HSPA8, PIK230 and PTPRB By similarity.By similarity

    Protein-protein interaction databases

    BioGridi202002. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z1M0.
    SMRiQ9Z1M0. Positions 35-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2525CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini47 – 334288ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini356 – 595240CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei26 – 4621Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei335 – 35521Helical; Name=2Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the P2X receptor family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG38999.
    GeneTreeiENSGT00390000016028.
    HOVERGENiHBG053086.
    InParanoidiQ9Z1M0.
    KOiK05220.
    OMAiLCPEYPT.
    OrthoDBiEOG78PV92.
    TreeFamiTF328633.

    Family and domain databases

    Gene3Di2.60.490.10. 1 hit.
    InterProiIPR003050. P2X7_purnocptor.
    IPR027309. P2X_extracellular_dom.
    IPR001429. P2X_purnocptor.
    [Graphical view]
    PANTHERiPTHR10125. PTHR10125. 1 hit.
    PTHR10125:SF13. PTHR10125:SF13. 1 hit.
    PfamiPF00864. P2X_receptor. 1 hit.
    [Graphical view]
    PRINTSiPR01314. P2X7RECEPTOR.
    PR01307. P2XRECEPTOR.
    TIGRFAMsiTIGR00863. P2X. 1 hit.
    PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z1M0-1 [UniParc]FASTAAdd to Basket

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    MPACCSWNDV LQYETNKVTR IQSTNYGTVK WVLHMIVFSY ISFALVSDKL    50
    YQRKEPVISS VHTKVKGIAE VTENVTEGGV TKLGHSIFDT ADYTFPLQGN 100
    SFFVMTNYVK SEGQVQTLCP EYPRRGAQCS SDRRCKKGWM DPQSKGIQTG 150
    RCVPYDKTRK TCEVSAWCPT EEEKEAPRPA LLRSAENFTV LIKNNIHFPG 200
    HNYTTRNILP TMNGSCTFHK TWDPQCSIFR LGDIFQEAGE NFTEVAVQGG 250
    IMGIEIYWDC NLDSWSHHCR PRYSFRRLDD KNTDESFVPG YNFRYAKYYK 300
    ENNVEKRTLI KAFGIRFDIL VFGTGGKFDI IQLVVYIGST LSYFGLATVC 350
    IDLLINTYSS AFCRSGVYPY CKCCEPCTVN EYYYRKKCES IMEPKPTLKY 400
    VSFVDEPHIR MVDQQLLGKS LQVVKGQEVP RPQMDFSDLS RLSLSLHDSP 450
    LTPGQSEEIQ LLHEEVAPKS GDSPSWCQCG NCLPSRLPEQ RRALEELCCR 500
    RKPGRCITTS KLFHKLVLSR DTLQLLLLYQ DPLLVLGEEA TNSRLRHRAY 550
    RCYATWRFGS QDMADFAILP SCCRWRIRKE FPKTEGQYSG FKYPY 595
    Length:595
    Mass (Da):68,388
    Last modified:July 27, 2011 - v2
    Checksum:iBE007FE195AF50A4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti11 – 111L → F in CAA08853. (PubMed:9849870)Curated
    Sequence conflicti221 – 2211T → A in CAA08853. (PubMed:9849870)Curated
    Sequence conflicti283 – 2831T → M in CAA08853. (PubMed:9849870)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ009823 mRNA. Translation: CAA08853.1.
    AC114632 Genomic DNA. No translation available.
    CCDSiCCDS19652.1.
    RefSeqiNP_035157.2. NM_011027.3.
    UniGeneiMm.42026.

    Genome annotation databases

    EnsembliENSMUST00000100737; ENSMUSP00000098303; ENSMUSG00000029468.
    GeneIDi18439.
    KEGGimmu:18439.
    UCSCiuc008zlq.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ009823 mRNA. Translation: CAA08853.1 .
    AC114632 Genomic DNA. No translation available.
    CCDSi CCDS19652.1.
    RefSeqi NP_035157.2. NM_011027.3.
    UniGenei Mm.42026.

    3D structure databases

    ProteinModelPortali Q9Z1M0.
    SMRi Q9Z1M0. Positions 35-355.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202002. 1 interaction.

    Chemistry

    BindingDBi Q9Z1M0.
    ChEMBLi CHEMBL5183.
    GuidetoPHARMACOLOGYi 484.

    PTM databases

    PhosphoSitei Q9Z1M0.

    Proteomic databases

    PaxDbi Q9Z1M0.
    PRIDEi Q9Z1M0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000100737 ; ENSMUSP00000098303 ; ENSMUSG00000029468 .
    GeneIDi 18439.
    KEGGi mmu:18439.
    UCSCi uc008zlq.1. mouse.

    Organism-specific databases

    CTDi 5027.
    MGIi MGI:1339957. P2rx7.

    Phylogenomic databases

    eggNOGi NOG38999.
    GeneTreei ENSGT00390000016028.
    HOVERGENi HBG053086.
    InParanoidi Q9Z1M0.
    KOi K05220.
    OMAi LCPEYPT.
    OrthoDBi EOG78PV92.
    TreeFami TF328633.

    Enzyme and pathway databases

    Reactomei REACT_198644. The NLRP3 inflammasome.

    Miscellaneous databases

    NextBioi 294114.
    PROi Q9Z1M0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Z1M0.
    Bgeei Q9Z1M0.
    Genevestigatori Q9Z1M0.

    Family and domain databases

    Gene3Di 2.60.490.10. 1 hit.
    InterProi IPR003050. P2X7_purnocptor.
    IPR027309. P2X_extracellular_dom.
    IPR001429. P2X_purnocptor.
    [Graphical view ]
    PANTHERi PTHR10125. PTHR10125. 1 hit.
    PTHR10125:SF13. PTHR10125:SF13. 1 hit.
    Pfami PF00864. P2X_receptor. 1 hit.
    [Graphical view ]
    PRINTSi PR01314. P2X7RECEPTOR.
    PR01307. P2XRECEPTOR.
    TIGRFAMsi TIGR00863. P2X. 1 hit.
    PROSITEi PS01212. P2X_RECEPTOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and functional characterisation of the mouse P2X7 receptor."
      Chessell I.P., Simon J., Hibell A.D., Michel A.D., Barnard E.A., Humphrey P.P.
      FEBS Lett. 439:26-30(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "ADP-ribosylation at R125 gates the P2X7 ion channel by presenting a covalent ligand to its nucleotide binding site."
      Adriouch S., Bannas P., Schwarz N., Fliegert R., Guse A.H., Seman M., Haag F., Koch-Nolte F.
      FASEB J. 22:861-869(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: ADP-RIBOSYLATION AT ARG-125 AND ARG-133 BY ART2B, MUTAGENESIS OF ARG-125 AND ARG-133.
    4. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-74.

    Entry informationi

    Entry nameiP2RX7_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z1M0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3