Q9Z1M0 (P2RX7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
P2X purinoceptor 7
Short name=P2X7

Alternative name(s):
ATP receptor
P2Z receptor
Purinergic receptor

Gene names

Name:	P2rx7
Synonyms:	P2x7

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.
Subunit structure	Functional P2XRs are organized as homomeric and heteromeric trimers. Interacts with LAMA3, ITGB2, ACTB, ACTN4, SVIL, MPP3, HSPA1, HSPCB, HSPA8, PIK230 and PTPRB By similarity.
Subcellular location	Cell membrane By similarity; Multi-pass membrane protein.
Post-translational modification	Phosphorylation results in its inactivation By similarity. ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel. Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface By similarity.
Sequence similarities	Belongs to the P2X receptor family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ion channel Ligand-gated ion channel Receptor
PTM	ADP-ribosylation Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NAD transport Inferred from mutant phenotype PubMed 17803959. Source: MGI T cell homeostasis Inferred from mutant phenotype PubMed 16116196. Source: MGI T cell proliferation Inferred from mutant phenotype PubMed 15914561. Source: MGI activation of MAPK activity Inferred from mutant phenotype PubMed 17306762. Source: MGI bleb assembly Inferred from mutant phenotype PubMed 17135244. Source: MGI cell morphogenesis Inferred from mutant phenotype PubMed 12055263. Source: MGI cell volume homeostasis Inferred from direct assay PubMed 18097031. Source: MGI cellular response to extracellular stimulus Inferred from mutant phenotype PubMed 18082965. Source: MGI ceramide biosynthetic process Inferred from direct assay PubMed 16325464. Source: MGI collagen metabolic process Inferred from mutant phenotype PubMed 16969386. Source: MGI cytolysis Inferred from direct assay PubMed 16116196PubMed 18097031. Source: MGI defense response to Gram-positive bacterium Inferred from mutant phenotype PubMed 11544318. Source: MGI homeostasis of number of cells within a tissue Inferred from mutant phenotype PubMed 16969386. Source: MGI inflammatory response Inferred from mutant phenotype PubMed 12055263PubMed 16969386. Source: MGI membrane budding Inferred from mutant phenotype PubMed 15905573. Source: MGI membrane depolarization Inferred from electronic annotation. Source: Compara membrane protein ectodomain proteolysis Inferred from mutant phenotype PubMed 17286969. Source: MGI mitochondrion organization Inferred from direct assay PubMed 16325464. Source: MGI multicellular organismal protein catabolic process Inferred from direct assay PubMed 11489964. Source: MGI negative regulation of MAPK cascade Inferred from direct assay PubMed 11489964. Source: MGI negative regulation of bone resorption Inferred from mutant phenotype PubMed 12677010. Source: MGI phagolysosome assembly Inferred from mutant phenotype PubMed 11544318. Source: MGI phospholipid transfer to membrane Inferred from mutant phenotype PubMed 12370338. Source: MGI phospholipid translocation Inferred from direct assay PubMed 18097031. Source: MGI plasma membrane organization Inferred from direct assay PubMed 18097031. Source: MGI pore complex assembly Inferred from mutant phenotype PubMed 12370338PubMed 15937334PubMed 16269410. Source: MGI positive regulation of T cell mediated cytotoxicity Inferred from mutant phenotype PubMed 14761980. Source: MGI positive regulation of apoptotic process Inferred from mutant phenotype PubMed 16325464PubMed 16493071PubMed 16969386. Source: MGI positive regulation of bone mineralization Inferred from mutant phenotype PubMed 12677010PubMed 18519738. Source: MGI positive regulation of calcium ion transport into cytosol Inferred from electronic annotation. Source: Compara positive regulation of cytolysis Inferred from electronic annotation. Source: Compara positive regulation of cytoskeleton organization Inferred from electronic annotation. Source: Compara positive regulation of gamma-aminobutyric acid secretion Inferred from mutant phenotype PubMed 15640761. Source: MGI positive regulation of glutamate secretion Inferred from mutant phenotype PubMed 15640761PubMed 17306762. Source: MGI positive regulation of interleukin-1 alpha secretion Inferred from mutant phenotype PubMed 18490713. Source: MGI positive regulation of interleukin-1 beta secretion Inferred from mutant phenotype PubMed 11016935PubMed 15905573PubMed 18490713. Source: MGI positive regulation of interleukin-6 production Inferred from mutant phenotype PubMed 11016935. Source: MGI positive regulation of mitochondrial depolarization Inferred from mutant phenotype PubMed 16198349. Source: MGI positive regulation of prostaglandin secretion Inferred from mutant phenotype PubMed 16269410. Source: MGI protein oligomerization Inferred from direct assay PubMed 15713258. Source: MGI protein processing Inferred from mutant phenotype PubMed 11016935. Source: MGI reactive oxygen species metabolic process Inferred from mutant phenotype PubMed 17448897. Source: MGI regulation of action potential in neuron Inferred from electronic annotation. Source: Compara regulation of sodium ion transport Inferred from electronic annotation. Source: Compara release of sequestered calcium ion into cytosol Inferred from mutant phenotype PubMed 18082965. Source: MGI response to ATP Inferred from direct assay PubMed 15240711PubMed 15713258. Source: MGI response to calcium ion Inferred from direct assay PubMed 15240711. Source: MGI response to drug Inferred from mutant phenotype PubMed 18082965. Source: MGI response to electrical stimulus Inferred from mutant phenotype PubMed 15640761. Source: MGI response to fluid shear stress Inferred from mutant phenotype PubMed 16269410. Source: MGI response to lipopolysaccharide Inferred from direct assay PubMed 15240711. Source: MGI response to mechanical stimulus Inferred from mutant phenotype PubMed 16269410. Source: MGI response to zinc ion Inferred from mutant phenotype PubMed 15978588PubMed 18082965. Source: MGI sensory perception of pain Inferred from mutant phenotype PubMed 15777864. Source: BHF-UCL skeletal system morphogenesis Inferred from mutant phenotype PubMed 18519738. Source: MGI synaptic vesicle exocytosis Inferred from mutant phenotype PubMed 15713258. Source: MGI
Cellular_component	bleb Inferred from electronic annotation. Source: Compara cell-cell junction Inferred from direct assay PubMed 15331634. Source: MGI cytoplasm Inferred from electronic annotation. Source: Compara external side of plasma membrane Inferred from direct assay PubMed 17286969. Source: MGI integral to nuclear inner membrane Inferred from electronic annotation. Source: Compara integral to plasma membrane Inferred from mutant phenotype PubMed 12677010. Source: MGI membrane raft Inferred from electronic annotation. Source: Compara neuromuscular junction Inferred from direct assay PubMed 15713258. Source: MGI neuronal cell body Inferred from direct assay PubMed 15964665PubMed 15978588. Source: MGI protein complex Inferred from electronic annotation. Source: Compara terminal bouton Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from mutant phenotype PubMed 12677010. Source: MGI copper ion binding Inferred from electronic annotation. Source: Compara extracellular ATP-gated cation channel activity Inferred from mutant phenotype PubMed 12677010PubMed 15713258. Source: MGI lipopolysaccharide binding Inferred from direct assay PubMed 11489964. Source: MGI magnesium ion binding Inferred from electronic annotation. Source: Compara purinergic nucleotide receptor activity Inferred from Biological aspect of Ancestor. Source: RefGenome zinc ion binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 595	595	P2X purinoceptor 7		PRO_0000161561
Regions
	Topological domain	1 – 25	25	Cytoplasmic Potential
	Transmembrane	26 – 46	21	Helical; Name=1; Potential
	Topological domain	47 – 334	288	Extracellular Potential
	Transmembrane	335 – 355	21	Helical; Name=2; Potential
	Topological domain	356 – 595	240	Cytoplasmic Potential
Amino acid modifications
	Modified residue	125	1	ADP-ribosylarginine; by ART2B
	Modified residue	133	1	ADP-ribosylarginine; by ART2B
	Modified residue	343	1	Phosphotyrosine By similarity
	Glycosylation	74	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	187	1	N-linked (GlcNAc...) Potential
	Glycosylation	202	1	N-linked (GlcNAc...) Potential
	Glycosylation	213	1	N-linked (GlcNAc...) Potential
	Glycosylation	241	1	N-linked (GlcNAc...) Potential
	Disulfide bond	119 ↔ 168		By similarity
	Disulfide bond	129 ↔ 152		By similarity
	Disulfide bond	135 ↔ 162		By similarity
	Disulfide bond	216 ↔ 226		By similarity
	Disulfide bond	260 ↔ 269		By similarity
Experimental info
	Mutagenesis	125	1	R → K: Abolishes calcium responses to NAD. Ref.3
	Mutagenesis	133	1	R → K: No effect on calcium responses to NAD. Ref.3
	Sequence conflict	11	1	L → F in CAA08853. Ref.1
	Sequence conflict	221	1	T → A in CAA08853. Ref.1
	Sequence conflict	283	1	T → M in CAA08853. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9Z1M0 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: BE007FE195AF50A4	FASTA	595	68,388
	10 20 30 40 50 60 MPACCSWNDV LQYETNKVTR IQSTNYGTVK WVLHMIVFSY ISFALVSDKL YQRKEPVISS 70 80 90 100 110 120 VHTKVKGIAE VTENVTEGGV TKLGHSIFDT ADYTFPLQGN SFFVMTNYVK SEGQVQTLCP 130 140 150 160 170 180 EYPRRGAQCS SDRRCKKGWM DPQSKGIQTG RCVPYDKTRK TCEVSAWCPT EEEKEAPRPA 190 200 210 220 230 240 LLRSAENFTV LIKNNIHFPG HNYTTRNILP TMNGSCTFHK TWDPQCSIFR LGDIFQEAGE 250 260 270 280 290 300 NFTEVAVQGG IMGIEIYWDC NLDSWSHHCR PRYSFRRLDD KNTDESFVPG YNFRYAKYYK 310 320 330 340 350 360 ENNVEKRTLI KAFGIRFDIL VFGTGGKFDI IQLVVYIGST LSYFGLATVC IDLLINTYSS 370 380 390 400 410 420 AFCRSGVYPY CKCCEPCTVN EYYYRKKCES IMEPKPTLKY VSFVDEPHIR MVDQQLLGKS 430 440 450 460 470 480 LQVVKGQEVP RPQMDFSDLS RLSLSLHDSP LTPGQSEEIQ LLHEEVAPKS GDSPSWCQCG 490 500 510 520 530 540 NCLPSRLPEQ RRALEELCCR RKPGRCITTS KLFHKLVLSR DTLQLLLLYQ DPLLVLGEEA 550 560 570 580 590 TNSRLRHRAY RCYATWRFGS QDMADFAILP SCCRWRIRKE FPKTEGQYSG FKYPY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and functional characterisation of the mouse P2X7 receptor." Chessell I.P., Simon J., Hibell A.D., Michel A.D., Barnard E.A., Humphrey P.P. FEBS Lett. 439:26-30(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"ADP-ribosylation at R125 gates the P2X7 ion channel by presenting a covalent ligand to its nucleotide binding site." Adriouch S., Bannas P., Schwarz N., Fliegert R., Guse A.H., Seman M., Haag F., Koch-Nolte F. FASEB J. 22:861-869(2008) [PubMed] [Europe PMC] [Abstract] Cited for: ADP-RIBOSYLATION AT ARG-125 AND ARG-133 BY ART2B, MUTAGENESIS OF ARG-125 AND ARG-133.
[4]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-74, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ009823 mRNA. Translation: CAA08853.1. AC114632 Genomic DNA. No translation available.
IPI	IPI00130271.
RefSeq	NP_035157.2. NM_011027.2.
UniGene	Mm.42026.
3D structure databases
ProteinModelPortal	Q9Z1M0.
SMR	Q9Z1M0. Positions 35-355.
ModBase	Search...
PTM databases
PhosphoSite	Q9Z1M0.
Proteomic databases
PaxDb	Q9Z1M0.
PRIDE	Q9Z1M0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000100737; ENSMUSP00000098303; ENSMUSG00000029468.
GeneID	18439.
KEGG	mmu:18439.
Organism-specific databases
CTD	5027.
MGI	MGI:1339957. P2rx7.
Phylogenomic databases
eggNOG	NOG38999.
GeneTree	ENSGT00390000016028.
HOVERGEN	HBG053086.
InParanoid	Q9Z1M0.
KO	K05220.
OMA	LCPEYPT.
OrthoDB	EOG4DJJWD.
Gene expression databases
ArrayExpress	Q9Z1M0.
Bgee	Q9Z1M0.
Genevestigator	Q9Z1M0.
GermOnline	ENSMUSG00000029468. Mus musculus.
Family and domain databases
Gene3D	2.60.490.10. 1 hit.
InterPro	IPR003050. P2X7_purnocptor. IPR027309. P2X_extracellular_dom. IPR001429. P2X_purnocptor. [Graphical view]
PANTHER	PTHR10125. PTHR10125. 1 hit. PTHR10125:SF13. PTHR10125:SF13. 1 hit.
Pfam	PF00864. P2X_receptor. 1 hit. [Graphical view]
PRINTS	PR01314. P2X7RECEPTOR. PR01307. P2XRECEPTOR.
TIGRFAMs	TIGR00863. P2X. 1 hit.
PROSITE	PS01212. P2X_RECEPTOR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q9Z1M0.
ChEMBL	CHEMBL5183.
NextBio	294114.
SOURCE	Search...

Entry information

Entry name

P2RX7_MOUSE

Accession

Primary (citable) accession number: Q9Z1M0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program